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Research

Methods portfolio

IFN-γ ELISpot

ELISpot
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With the help of an ELISpot assay (Enzyme Linked Immuno Spot Assay), individual reactive T cells can be functionally detected in a PBMC sample. The method is ideal for screening and for monitoring of successful vaccination.

Fluorescence-activated cell scanning (FACS)

FACS
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The phenotypic composition of a sample can be determined by fluorescence-activated cell scanning (FACS) measurement. Within a sample a variety of cellular markers can be used.

Luminex

A luminex measurement offers the possibility to determine 27 cytokines / chemokines simultaneously within a sample and measurement. As a result, cytokine profiles and chemokine profiles can be analyzed.

ELISA

An ELISA (Enzyme-linked Immunosorbent Assay) can be used for a wide variety of approaches. In our laboratory we use the ELISA to detect specific antibody reactions in the blood serum and to determine relative antibody titers.

TCR-sequencing

Each T cell expresses only one T cell receptor (TCR). With the help of TCR sequencing, the TCR repertoire at the time of blood or tumor sampling can be analyzed and, if necessary, followed over time. This enables detection of clonal expansion of antigen-specific T cells.

Novel high-throughput technologies allow combined detection of TCR, gene expression profile and additional features for > 10.000 individual cells, allowing for unprecedented insight into therapie-induced changes and tumor-immune interactions.