WO1997002028A1

WO1997002028A1 - Inactivation of pathogens using hydroxymethylamines

Info

Publication number: WO1997002028A1
Application number: PCT/US1996/011152
Authority: WO
Inventors: Richard F. Rossi; Donald L. Heefner; Charles M. Zepp
Original assignee: Hemasure, Inc.
Priority date: 1995-06-29
Filing date: 1996-07-01
Publication date: 1997-01-23
Also published as: BR9606454A; CA2198085A1; JPH10506927A; EP0792146A1; AU7717396A

Abstract

A method of inactivating viruses that may be present in a whole blood or blood product intended for administration to an individual is disclosed. The whole blood or blood product sample is treated with an effective quantity of a hydroxymethylamine of formula (I). An exemplary hydroxymethylamine is N-hydroxymethylglycine and salts thereof.

Description

INACTIVATION OF PATHOGENS USING HYDROXYMETHYLAMINES

FIELD OF THE INVENTION

This invention relates to methods for inactivation of pathogens.

BACKGROUND OF THE INVENTION

Spread of infectious disease resulting from transfusion of contaminated blood, administration of contaminated blood products or handling or usage Of objects that have come into contact with contaminated blood and/or blood products-Aas been well documented and is recognized as a major public health^' concern. Most notably, transmission of viral hepatitis and/or Acquired Immune Deficiency Syndrome (AIDS) through contaminated blood and blood products has received widespread attention. However, viral hepatitis and AIDS are only two of the many diseases that can be spread through use of contaminated blood and blood produc-fes. Lesser known pathogens, such as T-cell lymphotropic viruses (Types I and II) , cytomegalovirus, Epstein-Barr virus, the parvoviruses and P2as-nodiu-τι (malaria-causing) protozoa, may also be spread through contaminated blood and blood products. In addition, still other microorganisms that have not yet even been identified or recognized as being pathogenic .may be transmitted through contaminated blood and blood products and, therefore, similarly pose a serious public health risk. The HIV virus is illustrative of a pathogen that, until recently, was not even recognized. Today, there are over 10 million people worldwide who have contracted AIDS, many of these people having contracted the disease through use of infected blood or blood products; however, less than two decades ago, AIDS was not even a recognized disease. Thus, it is clear that there is a great need for a method for effectively inactivating pathogens in blood and blood products.

In response to this need a number of techniques have been devised for inactivating pathogens, particularly infectious viral agents, in blood and/or blood products. A review of many of these techniques is presented in Suomela, "Inactivation of Viruses- in Blood and Plasma Products, " Transfusion Medicine

Reviews, Vol. VII, No. 1, pp. 42-57 (January 1993), which is incorporated herein by reference.

One such technique which has been used to inactivate viruses in blood and/or blood products is pasteurization. [See Burnouf-Radosevich et al. , "A Pasteurized Therapeutic Plasma, " Infusionstherapie. J 9:91-94 (1992)] The pasteurization of blood and/or blood products is most often effected by heating them in the liquid state for 10 hours at 60°C. A small amount of protein stabilizer, such as caprylate or tryptophanate, is often added to the preparation. After pasteurization has been completed, the stabilizer typically must be removed from the preparation prior to its clinical use. As is the case with many of the existing viral inactivation techniques discussed herein, pasteurization is more effective in inactivating enveloped viruses (i.e., viruses having a lipid envelope surrounding the viral capsid) than in inactivating non-enveloped viruses (i.e., viruses which lack a lipid envelope surrounding the viral capsid) . Another technique which has been used to inactivate viruses in blood and/or blo^d products is the solvent/detergent (S/D) method. [See, for example, Hellstern et al. , "Manufacture and in vitro Characterization of a Solvent/Detergent-Treated Human Plasma, " Vox Sang, 63:178-185 (1992); Horowitz et al . , "Solvent/Detergent-Treated Plasma: A Virus- Inactivated Substitute for Fresh Frozen Plasma, " Blood, 79:826-831 (1992) ; and Piquet^' et al. , "Virus Inactivation of Fresh Frozen Plasma by a Solvent

Detergent Procedure: Biological Results, Vox Sanσ. 63:251-256 (1992) .] The S/D method, which is limited to use in inactivating enveloped viruses, Involves treating a blood preparation with an organic mixture which disrupts the lipid envelope of enveloped viruses . The disruption of the lipid envelope leads either to complete structural disruption of the virus or to destruction of the cell receptor recognition site on the virus. In either case, the virus is rendered noninfectious. The solvent used in the S/D method is most often tri- (n-butyl)phosphate (TNBP) , and the detergent is either Tween 80°, Triton X-100^C or sodium deoxycholate. Temperature and time influence the efficacy of the S/D method, typical temperatures being in the range of 24° C to 37°C, and the typical duration of treatment being at least 6 hours.

Still another technique which has been used to inactivate viruses in blood and/or blood products is photochemical inactivation. [See Mohr et al. , "Virus Inactivated Single-Donor Fresh Plasma Preparations, " Infusiontherapie, 19:79-83 (1993) ; Wagner et al. , "Differential sensitivities of viruses in red cell suspensions to methylene blue photosensitization, " Transfusion, 34 (6) :521-526 (1994); Wagner et al. , "Red cell alterations associated with virucidal methylene blue phototreatment, " Transfusion, 33:3_*0-36 (1993); Mohr et al. , "No evidence for neoantigens in human plasma after photochemical virus inactivation, " Ann. Hematol., 65:224-228 (1992); Lambrecht et al. , Photoinactivation of Viruses in Human Fresh Plasma by Phenothiazine Dyes in Combination with Visible Light," Vox Sana, 60:207-213 (1991), Goodrich et al., "Selective inactivation of viruses in the presence of human platelets: UV sensitization with psoralen derivatives," Proc. Nat. Acad. Sci. USA, 91:5552-5556 (1994); Virus Inactivation in Plasma Products, J.-J Morgenthaler, ed. Karger, NY (1989) ; and BioWorld Today, Vol. 4, No. 229, pages 1 and 4 (November 24, 1993) .] The photochemical inactivation of a blood preparation typically involves treating the blood preparation with a photoactivatable chemical and then irradiating the preparation with light of a sufficient wavelength to activate the photoactivatable chemical. Examples of photoactivatable chemicals used in the photochemical inactivation of viruses present in blood preparations include psoralens, hypericin, methylene blue and toluidine blue. It is believed that psoralens, which have an affinity for nucleic acids, inactivate viruses by intercalating between viral nucleic acid base pairs and, in the presence of UVA light, forming a covalent bond with the viral nucleic acid, thereby preventing its transcription and/or replication. The manner in which hypericin, methylene blue and toluidine blue inactivate viruses is not as well- defined as that for psoralens. However, it is believed that these chemicals, when photoactivated, generate the highly reactive entity, singlet oxygen, which then attacks the cellular structure (e.g. viral envelope) of the virus.

Whereas photochemical inactivation has been largely successful in inactivating enveloped viruses, it has been largely unsuccessful in inactivating non- enveloped viruses. The failure of photochemical inactivation to inactivate non-enveloped viruses is significant since Poliovirus, Adenovirus, Hepatitis A and Parvovirus (Parvo B1 ) are among those non- enveloped viruses that are pathogenic to humans.

It should be noted that photochemical inactivation of the type described above has been most successful when applied to inactivating viruses in blood preparations lacking red blood cells (e.g., plasma) . This is because blood preparations that include red blood cells typically absorb light at the same wavelengths used to photoactivate the chemicals.

Viral inactivation agents are substances that render viruses incapable of replication and proliferation. From the literature discussed above, one may conclude that viral inactivating compounds have been identified which are specifically toxic to blood borne viruses such that cells and proteins are not adversely affected. Still it is important to limit exposure of biological samples to viral inactivation agents, such as, for example, psoralens, hypericin, methylene blue, toluidine blue or a combination of tri- (n-butyl) phosphate and a detergent such as Tween 80, Triton X-100 or sodium deoxycholate to the minimum extent necessary to reduce potentially signification interactions that could lead to undesirable side effects . U.S. Patent 4,337,269 (Berke et al. ) disclosed a biocidal composition containing a compound, hydroxymethylaminoacetate (also referred to herein as hydroxymethylglycinate) , which is produced by the reaction of glycine or a salt of glycine with formaldehyde. In the aforementioned patent, hydroxymethylglycinate is said to be effective at inhibiting the growth of bacteria, yeasts and molds in a variety of substances susceptible to microbial contamination, such as cosmetics, foodstuffs, pharmaceuticals, paints, cutting oils or fluids, agricultural products, oil .drilling fluids, paper industry, embalming solutions, cold sterilization medical and dental equipment, cooling towers, fabric impregnation, latexes, swimming pools, inks, household disinfectants, waxes and polishes, toilet bowl cleaners, bathroom cleaners, laundry detergents, soaps, wood preservatives, hospital and medical antiseptics and adhesives.

Sodium hydroxymethylglycinate is the active ingredient in the preservative SUTTOCIDE™A, which is commercially available from Sutton Laboratories, Chatham, New Jersey. In certain promotional literature published by Sutton Laboratories, SUTTOCIDE™A is said to be active against Gram- negative and Gram-positive bacteria, yeast and mold and is suggested for use as a preservative in shampoos, hair conditioners and facial treatments.

U.S. Patent 4,980,176 (Berke et al . ) disclosed a composition containing one or more 3-isothiazolones and a compound which is a member selected from the group consisting of hydroxymethyl-aminoacetic acid, its salts and lower alkyl esters. The aforementioned composition is described in the patent as being effective against bacteria, yeasts and molds. Suggested applications in the patent for the above- described composition include use as a preservative in cosmetics, toiletries and household cleaning products, use as a biocide for synthetic latexes, emulsion paints and other coatings, adhesives, polishes, carpet backing compositions, surfactants, metalworking fluids, industrial and domestic water treatment including cooling tower systems and swimming pools, adhesive mats, drilling mud formulations, painting pastes, spin finish emulsions, polymer dispersions and fuels and as a slimicide for slime control in the manufacture of paper from wood pulp.

It is to be noted that nowhere in the foregoing patents or publications is it taught that the biocidal activity of hydroxymethylaminoacetic acid, its salts and/or lower alkyl esters can be extended beyond bacteria, yeasts and molds to include viruses. Moreover, because most biocidal agents that are effective against bacteria, yeasts and molds are not effective against viruses, the foregoing patents and publications do not provide any reasonable basis for one of ordinary skill in the art to expect that hydroxymethylaminoacetic acid and/or its derivatives would be effective in inactivating viruses.

It is also to be noted that nowhere in the foregoing patents or publications is it taught that hydroxymethylaminoacetic acid and/or its derivatives can be used in blood and/or blood products. Moreover, because most biocidal agents that are effective against bacteria, yeast and/or molds cannot be used to inactivate such pathogens in blood an/or blood products without adversely affecting the suitability of the treated blood and/or blood product for subsequent administration to a patient (due to their toxicity and/or their reactivity with plasma proteins and certain other blood constituents) , the foregoing patents and publications do not provide any reasonable basis for one of ordinary skill in the art to expect that hydroxymethylaminoacetic acid and/or ies derivatives could be used in blood and/or blood products without rendering the treated blood and/or blood product unsuitable for.- subsequent administration to a patient in need thereof.

Also of interest is Japanese Published Application No. 62-195304, which disclosed that paraform (84%, 25g) was added to 98% diethanolamine (332g) at 40 degrees, and then stirred at 50-60 degrees for 1 hour to yield hydroxymethyldi- -athanolamine. It is also disclosed that hydroxymethyl-diethanolamine, at 300-500ppm, controlled Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Proteus vulgaris and P. mirabilis on nutrient agar plates. It is to be noted, however, that nowhere in the foregoing Japanese application is it taught or suggested that hydroxymethyldiethanolamine has virucidal activity or that hydroxymethyldiethanol¬ amine could be used in blood and/or blood products without adversely affecting the suitability of the treated blood and/or blood product for subsequent administration to a patient in need thereof.

Therefore, a need exits for a method for treating blood which inactivates viruses without adversely affecting the suitability of the treated blood and/or blood product for subsequent administration to an individual in need thereof.

SUMMARY OF THE INVENTION The present invention is a method of inactivating a virus in a biological fluid, such as blood. In the method, the biological fluid is contacted with a hydroxymethylamine (HMA) in sufficient quantity to inactivate the virus (i.e.-, an effective amount) . The biological fluid can be of any type including, but not-_/limited to, whole blood and a wide variety of blood components, including, but not limited to, red blood cells, red blood cell concentrate, platelets, platelet concentrate, platelet rich plasma, platelet poor plasma, source plasma (plasmaphoresis plasma) , fresh frozen plasma, plasma proteins (e.g., clotting factors VIII, X, etc.) , and other body fluids, such as lymph, cerebrospinal fluid, semen, saliva, etc. While targeted at the inactivation of viruses, the method is effective against other microorganisms as well. These microorganisms can be pathogenic or nonpathogenic and include bacteria, yeasts, molds and protozoa.

In one embodiment, this invention provides a method for inactivating a microorganism contained in a biological fluid. The method comprises the step of contacting the microorganism with an effective amount of a hydroxymethylamine (HMA) . Suitable hydroxy- methylamines include compounds of Formula (I) R \

N-CH,-OH (I)

R¹ wherein:

R is chosen from the group consisting of hydrogen, alkyl, aryl, substituted alkyl, substituted aryl; and R¹ is chosen from the group consisting of acid-, amide-, hydroxy- or mercapto-functional alkyl; acid- , amide-, or hydroxy-functional aryl; acid-, amide-, or hydroxy-functional substituted alkyl; and acid-, amide-, or hydroxy-functional substituted aryl; or R and R¹ may be joined together to form an acid, amide or hydroxy-functional heterocyclic structure.

Preferred HMAs are those in which the functional group is an amide or an acid selected from the group consisting of carboxylate, phosphate, phosphonate, sulfate and sulfonate. Carboxylic acids are particularly preferred.

Preferred individual hydroxymethylamines having the amide functionality include hydroxymethylglycin- amide, hydroxymethylpenicillinamide, hydroxymethyl- leucinamide, hydroxymethylacrylamide and hydroxy- methylnicotinamide. Preferred hydroxymethylamines having the acid functionality include hydroxymethyl- glycine, hydroxymethylphosphonomethylglycine, hydroxymethyl-p-aminohippuric acid, hydroxy- methylpropargylglycine, hydroxymethyl-o- phosphothreonine, hydroxymethylaminoadipic acid, hydroxymethyl-o-phosphoserine, hydroxymethylamino- ethylphosphonic acid, hydroxymethylleucine, hydroxymethyl-β-alanine, hydroxymethylcysteine, hydroxymethylfolic acid, hydroxymethylamino- phosphonobutyric acid, hydroxymethylphenylalanine, hydroxymethylaminophenylacetic acid, hydroxymethyl-o- phosphorylethanolamine, hydroxymethylalanine, hydroxymethylserine, hydroxymethylvaline, hydroxymethylmethionine, hydroxymethylglutamic acid, hydroxymethylaspartic acid, hydroxymethyllysine, hydroxymethylproline, hydroxymethylmercaptopropionyl- glycine, hydroxymethylaminoethyl hydrogen sulfate, hydroxymethylpenicillamine, hydroxymethylornithine, and hydroxymethylcysteine. Preferred hydroxymethyl- amines having neither an acid nor an amide functionality include hydroxymethylmercaptoethyl- amine, hydroxymethylaminoethanol, hydroxymethyl- aminopropanol and hydroxymethyldiethanolamine.

A particularly preferred HMA is hydroxymethyl- glycine or a salt thereof.

According to the method of the invention, the hydroxymethylamine and biological fluid are preferably combined to produce a final concentration of hydroxymethylamine of approximately 0.05 % - 3.0 % by weight; the contact time is from 0.5 hours to 4 hours, and the temperature is maintained between about 4° C and about 30° C.

In another aspect, the invention relates to a method of processing a biological fluid intended for administration to an individual in need thereof. The method comprises the steps of: (a) treating the biological fluid with an effective amount of a pathogen-inactivating hydroxymethylamine, thereby producing a treated biological fluid; and (b) then removing free hydroxymethylamine from the treated biological fluid. In another aspect the invention relates to a method of treating an individual in need of a biological fluid. The method comprises the steps" of: (a) treating the biological fluid with an effective amount of a pathogen-inactivating hydroxymethylamine, thereby producing a treated biological fluid; and (b) administering the treated biological fluid to the individual in need thereof.

In another aspect, the invention relates to a method of treating a biological fluid. The method comprises combining an effective amount of a virus- inactivating hydroxymethylamine with the biological fluid, whereby at least about a 10-fold reduction in plaque forming units of virus is realized. Subsequently, the virus-inactivating compound can be removed from the biological fluid prior to its administration to an individual. If the biological fluid is blood, the treated blood can be returned to the individual from whom it was obtained. Alternatively, the treated blood can be stored and administered later in time to the same individual or another individual in need thereof.

The method of the invention can also be used to inactivate pathogens present in bodily fluids other than blood, and to disinfect medical instruments and analytical equipment that have come into contact with potentially contaminated blood. Similarly, the method of the invention can also be used to disinfect blood samples that are not intended for subsequent administration to an individual, but rather, are intended for subsequent chemical analysis. Other possible applications of the invention are apparent to those skilled in the art. Additional objects, as well as features and advantages, of the present invention will be set forth in part in the detailed description which follows, and in part will be obvious from the detailed description or may be learned by practice of the invention. Various embodiments of the inventions will be described in sufficient detail to enable those skilled in the art to practice the invention, and it is to be understood that other embodiments may be utilized and that changes may be made without departing from the scope of the invention. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is best defined by the appended claims.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to the method of use of HMA' s as pathogen-inactivating agents, particularly viral-inactivating agents, in biological samples, such as blood or blood products.

Use of HMA for treatment of pathogens

The present invention is based, in part, on the unexpected discovery that a HMA can be used to inactivate viruses in biological samples. The present invention is also based, in part, on the unexpected discovery that hydroxymethylamines such as those of Formula (I) , can be used to inactivate viruses in blood and/or blood products, without rendering the treated blood and/or blood products unsuitable for subsequent administration to an individual. Suitable pathogen-inactivating (e.g., virus- inactivating) HMA' s for use in the invention can be readily selected by those skilled in the art using art-recognized methods and the test methodology set forth in the accompanying Examples. For the purposes of the specification and claims, an HMA that reduces the number of plaque-forming units (PFU) in a biological fluid by a factor of at least 10 (i.e. one log reduction) is a pathogen-inactivating HMA. The ability to reduce the number of PFUs can be asse&sed using art-recognized methods, one example of which is described in Example 1. If -an HMA reduces the number of PFUs by at least one log unit, as assessed by the method described in Example 1, it is a pathogen- inactivating HMA. The term "hydroxymethyl" when used herein in connection with a particular amino compound, designates that the compound has a -CH₂OH substituent on the amino group.

Suitable.,alkyl groups in compounds of Formula (I) include straight chain, branched chain, and cyclic alkyl groups, containing one to about 22 carbon atoms, more preferably one to about 12 carbon atoms. When the alkyl group is a cyclic alkyl, 3-, 4- 5-, 6-, and 7- membered rings are preferred.

Suitable aryl groups in compounds of Formula (I) include hydrocarbon aryl groups containing a 6- membered aromatic ring, such as phenyl, fused bicyclic systems such as a- and 3-naphthyl, histidine, indenyl, tetralinyl, and the like, and monocyclic and polycyclic heteroaryl groups containing a 5- or 6- membered heteroaromatic ring, e.g., pyridyl, pyrimidinyl, quinolinyl, furanyl, thienyl, isothiazolyl, isoxazolyl, imidazolyl, 1H- pyrrolyl, indolyl, purinyl, and the like.

Suitable substituents in a substituted alkyl or substituted aryl group include halogen (e.g., fluoro, chloro, bromo), hydroxy, alkoxy (e.g., alkoxy containing one to 8 carbon atoms), alkylthio (e.g., alkylthio wherein the alkyl group contains one to 8 carbon atoms), lower alkyl (i.e., alkyl containing one to four carbon atoms), cycloalkyl (e.g., cydopropyl, cyclopentyl, cyclohexyl) , phenyl, benzyl, benzo, mercapto, or combinations thereof.

R and R. of Formula (I) can together form an N- heterocyclic structure (i.e., a cyclic structure wherein the hydroxymethylated nitrogen is an atom in the cyclic structure) , such as a proline, pyrrolidine, piperidine, 2-pyrroline, indoline, aziridine, azetidine, and the like.

In a preferred embodiment, the HMA contains a carboxylic acid portion as part of at least one of the substituted alkyl or substituted aryl groups, so that the HMA falls within the broad class of N- hydroxymethylated aminoacids. The aminoacid is not restricted to naturally occurring α-aminoacids, although they provide particularly convenient starting materials, and their residues are optimally biocompatible.

When the functional group is acidic, salts of the HMA, such as metal salts (e.g. sodium and potassium salts) , and ammonium salts may be used. Preferred HMAs include hydroxymethylglycine, hydroxymethylphosphonomethylglycine, hydroxymethyl-p- aminohippurate, hydroxymethylpropargylglycine, hydroxymethyl-o-phosphothreonine, hydroxymethyl- aminoadipate, hydroxymethyl-o-phosphoserine, hydroxymethylamino-ethylphosphonic acid, hydroxymethylleucine, hydroxymethyl-/-^*-alanine, hydroxymethylcysteine, hydroxymethylfolate, hydroxymethylaminophosphonobutyric acid, and hydro- xymethylphenylalanine, and their corresponding salts. Hydroxymethylglycine, hydroxymethylfolate, hydroxymethylaminophosphonobutyric acid, hydroxymethylpropargylglycine and hydroxymethyl-o- phosphothreonine are particularly preferred.

Other suitable HMAs include hydroxymethylamino- phenylacetic acid, hydroxymethyl-o-phosphorylethanol- amine, hydroxymethylalanine, hydroxymethylserine, hydroxymethylvaline, hydroxymethylmethionine, hydroxymethylglutamate, hydroxymethylaspartate, hydroxymethyllysine, hydroxymethylproline, hydroxymethylmercaptopropionylglycine, hydroxymethyl- mercapto-ethylamine, hydroxymethylaminoethyl hydrogen sulfate, hydroxymethylamino-ethanol, hydroxymethyl- penicillamine, hydroxymethylhydantion, hydroxymethyl- ornithine, hydroxymethylcysteine, hydroxymethylamino¬ propanol, hydroxymethyldiethanolamine, and their corresponding salts.

HMAs can be readily synthesized by those skilled in the art. For example, to synthesize a hydroxymethyl-aminoacid, one equivalent each of the corresponding amino acid, formalin and sodium hydroxide are combined. One application of this invention is a method of inactivating a pathogen present in a biological sample, such as blood or a blood product intended- for administration to an individual in need thereof, said method comprising the step of treating the biological sample, such as blood or a blood product with an effective amount of a pathogen-inactivating acid- or hydroxy-functional HMA.

In accordance with the teachings of the present invention, treatment of a biological fluid, such as blood and/or a blood product' comprises combining an appropriate quantity of a pathogen-inactivating HMA with the biological sample and then allowing the sample to incubate for an appropriate period of time at a suitable temperature. The final concentration of the HMA in the sample is preferably approximately 0.05%-3.0%, more preferably approximately 0.5%. The incubation period is sufficiently long to inactivate pathogen in the sample, commonly from about 0.5 hour to about 4 hours, conventiently about 1 hour, and the incubation temperature is about 18^*C to about 37^*C, more preferably about 20^*C to about 30^*C. In a specific embodiment, the treatment of a blood and/or blood product comprises combining sodium hydroxy- methylglycinate with the sample to give a final sodium hydroxymethylglycinate concentration of 0.5%, then allowing the sample to incubate for approximately 60 minutes at a temperature of about 30^*C.

The above-described method can be used to inactivate viruses, bacteria, molds, yeasts, protozoa and other pathogens in biological samples, such as whole blood and a wide variety of blood components, including, but not limited to, whole blood, red blood cell component, red blood cell concentrate, platelet component, platelet concentrate, platelet rich plasma, platelet poor plasma, source plasma, fresh frozen plasma and plasma proteins. As mentioned above, one advantageous aspect of the present method is that it does not render a biological sample unsuitable for subsequent administration (e.g., transfusion) to an individual.

It is believed that HMA' s inactivate viruses either by reacting with the/protein coat or with the component nucleic acids.

The method of the invention can also be used to inactivate pathogens present in bodily fluids other than blood, and to disinfect medical instruments and analytical equipment that have come into contact with potentially contaminated biological samples, such as blood. Similarly, the method of the invention can be used to disinfect blood samples that are not intended for subsequent administration to an individual, but rather, are intended for subsequent chemical analysis. Other possible applications of the invention are apparent to those skilled in the art.

The following examples are illustrative only and should in no way limit the scope of the present invention.

EXAMPLE 1

Five microliters of T4 (American Type Culture Collection No. 11303-B4) virus stock and 14 μL of blood plasma were added to each of four tubes. 1 μL of a 50% stock solution of sodium hydroxymethylglycinate (International Specialty Products, Bound Brook, New Jersey) was added to a first tube, 1 μL of a 10 mg/mL stock solution of - diazolidinyl urea (a bactericide) was added to a second tube, 1 μL of a 10 mg/mL stock solution of imidazolidinyl urea (a bactericide) was added to a third tube, and 1 μL of Phosphate Buffered Saline (PBS) was added to the fourth tube. The mixtures were incubated at room temperature for 1 hour before sampling, diluting, mixing with host cells in sof-t agar and pouring onto solid medium. After overnight incubation at 37^*C, the plagues were counted. The results are summarized below in TABLE I .

TABLE I

Sample Inhibitor Concentration PFUs (Plague in Mixture Forming Units)

1 Hydroxymethylglycinate 2.5% 22x10"

2 Diazolidinyl urea 0.5 mg/mL 14x10^s

3 Imidazolidinyl urea 0.5 mg/mL 42X10⁸

4 None 0 66x10^s

The above results show that, whereas the bactericides diazolidinyl urea and imidiazolidinyl urea were ineffective at the above-indicated concentrations at inactivating T4 virus, hydroxymethylglycinate exhibited strong viricidal activity.

EXAMPLE 2

Five microliters of T4 virus stock were added to each of fourteen tubes. Fourteen microliters of blood plasma were added to each of seven of the tubes, and 14 μL of whole blood were added to each of' the remaining seven tubes. One microliter of an appropriate hydroxymethylglycinate stock solution was added to each of six of the plasma-containing tubes and to each of six of the whole blood-containing tubes to give the below-listed concentrations. One microliter of PBS was added to each of the remaining tubes . After incubation at room temperature for 1 hour, samples from each tube were taken, diluted, mixed with host cells, and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE II.

TABLE II

Sample Hydroxymethylglycinate Plasma PFU Concentration or Whole

Blood

1 -έ_. . O"o Plasma 21xl0⁴

2 1.25% Plasma 71xl0⁴

3 0.625% Plasma 70xl0⁴

4 0.25% Plasma 58xl0⁵

5 0.125% __ Plasma 36xl0⁶

6 0.063% Plasma 18xl0⁷

7 0% Plasma 60xl0⁸

8 2.5% Whole 29xl0⁴ Blood

9 1.25% Whole 50xl0⁴ Blood

10 0.625% Whole 76x10" Blood

11 0.25% Whole 43x10^s Blood

12 0.125% Whole 74xl0⁶ Blood

13 0.063% Whole 21xl0⁸ Blood

14 0% Whole 67xl0⁸ Blood As can be seen from the results above, the inactivation of T4 was dependent upon the concentration of hydroxymethylglycinate. A concentration of 0.625% hydroxymethylglycinate led to a 4 log decrease in T4 over a period of 1 hour at room temperature.

EXAMPLE 3

Two hundred twenty-five microliters of T4 stock were added to each of twelve tubes. 4.75 μL of whole blood were added to three of the tubes, 4.75 μL of plasma were added to another^" three of the tubes and 4.75 μL of PBS were added to still another three of the tubes, the remaining three tubes serving as controls. 25 μL of a 50% solution of hydroxymethylglycinate were added to each of the tubes, except for the controls. The various mixtures were incubated at room temperature, and at the times indicated below samples were taken, diluted, mixed with hβst cells and overlaid onto solid medium. After overnight incubation at 37"C, plaques were counted. The results are summarized below in TABLE III.

TABLE III

Sample Hydroxymethylglycinate Incubation PFU's- Present Time

Whole Yes 15 minutes 27X10⁷ Blood

Plasma Yes 15 minutes 9X10⁷

PBS Yes 15 minutes 25x10^s

Control No 15 minutes ND

Whole Yes 30 minutes 9x10⁷ Blood

Plasma Yes 30 minutes 23X10⁶

PBS Yes 30 minutes 40xl0⁵

Control No 30 minutes ND

Whole Yes 60 minutes 14x10^s Blood

Plasma Yes 60 minutes 25X10⁵

PBS Yes 60 minutes 14X10⁴

Control No 60 minutes 50X10⁷

As can be seen from the results above, higher levels of hydroxymethylglycinate are required to inactivate T4 in blood or in plasma than in buffer solution. As can also be seen, the efficacy of hydroxymethylglycinate appears to increase as incubation time increases.

EXAMPLE 4 Thirty microliters of T4 stock and 465 μL of blood plasma were added to each of ten tubes. 5 μL of a 50% stock solution of hydroxymethylglycinate were added to each of eight of the ten tubes to give a final hydroxy-methylglycinate concentration of 0.5%. The mixtures were incubated at the temperatures indicated below. At the times indicated below, samples were taken from each tube, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation, plaques were counted. The results are summarized below in TABLE IV.

TABLE IV

Incubation Hydroxymethylglycinate Incubation PFU's Time Present Temperature

30 minutes Yes 5°C 11X10⁷

30 minutes Yes 21°C 10xl0⁶

30 minutes Yes 30°C 16X10⁴

30 minutes Yes 37°C 22X10³

30 minutes No 37°C ND

60 minutes Yes 5°C 12x10^s

60 minutes Yes 21°C 23X10⁴

60 minutes Yes 30°C < 10³

60 minutes Yes 37°C < IO³

60 minutes No 37°C 80X10⁷

As can be seen from the above results, the inactivation of T4 in blood plasma using 0.5% hydroxymethylglycinate is temperature dependent. Significantly greater viral toxicity was seen at 30^*C than at room temperature, and at 5^*C only a 1 log drop in viable virus was observed.

EXAMPLE 5

Thirty microliters of T4 stock and 465 μL of packed red blood cells were added to each of ten tubes. 5 μL of a 50% stock solution of hydroxymethylglycinate were added to each of eight of the ten tubes to give a final hydroxymethylglycinate concentration of 0.5%. The mixtures were incubated at the temperatures indicated below. At the cimes indicated below, samples were taken from each tube, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37°C, plaques were counted . The results are summarized below in TABLE V.

TABLE V

Incubation Hydroxymethylglycin te Incubation PFU'S Time Present Temperature

30 minutes Yes 5°C 15X10⁷

30 minutes Yes 21°C 20x10^s

30 minutes Yes 30°C 11x10^s

30 minutes Yes 37°C 21xl0⁴"

30 minutes No 37°C ND

60 minutes Yes 5°C 44x10^s

60 minutes Yes 21°C 10x10^s

60 minutes Yes 30°C 24x10*

60 minutes Yes ^•37°C < 10³

60 minutes No 37°C 11x10^s

As can be seen from the above results, the inactivation of T4 in packed whole blood is also temperature dependent. At 37°C, over a 5 log drop in viral viability was observed over the time of the experiment. At 5°C, the decrease in viral viability was only 2 logs.

EXAMPLE 6

Thirty microliters of T4 stock and 440 μL of blood plasma were added to each of twelve tubes. 30 μL of a 50% stock solution of hydroxymethylglycinate were added to each of ten of the twelve tubes to give a final hydroxymethylglycinate concentration of 3%.

The mixtures were incubated at the temperatures indicated below. At the times indicated below, samples were taken from each tube, diluted, mixed with host cells and overlaid onto solid medium.

After overnight incubation at 37°C, plaques were counted. The results are summarized below in Table VI.

TABLE VI

Incubation Hydroxymethylglycinate Incubation PFU's Time Present Temperature

30 minutes Yes 0°C 29X10⁷

30 minutes Yes 5°C 72x10^s

30 minutes Yes 21°C 69X10⁵

30 minutes Yes 30°C < IO^{3 *}

30 minutes Yes 37°C < IO³

30 minutes No 37°C ND

60 minutes Yes 0°C 28X10⁷

60 minutes Yes 5°C 35x10^s

60 minutes Yes 21°C 75X10³

60 minutes Yes 30°C < 10³

60 minutes Yes 37°C < 10³

60 minutes No 37°C 117X10⁷

As can be seen by comparing the above results to those obtained in Examples 4 and 5, 3% hydroxymethylglycinate possesses greater viricidal activity than does 0.5% hydroxymethylglycinate. The above results also indicate that viral inactivation by 3% hydroxymethylglycinate is temperature dependent. For example, a greater than 5 log decrease in viable virus was observed when the incubation temperature was increased from 0°C to 30°C.

EXAMPLE 7

Fifty microliters of an overnight culture of E. coli and 465 μL of blood plasma were added to each of ten tubes. 5 μL of a 50% stock solution of hydroxymethylglycinate were added to each of eight of the ten tubes to give a final hydroxymethylglycinate concentration of 0.5%. The mixtures were incubated at the temperatures indicated below. At the times indicated below, samples were taken from each tube, diluted, and plated. After overnight incubation at 37°C, colonies were counted. The results are summarized below in TABLE VII.

TABLE VII

Incubation Hydroxymethylglycinate Incubation Colonies Time Present Temperature

30 minutes Yes 5°C 7xl0⁷

30 minutes Yes 21°C 11x10^s

30 minutes Yes 30°C 15xl0⁵

30 minutes Yes 37°C < IO³

30 minutes No 37°C ND

60 minutes Yes 5°C 41x10^s

60 minutes Yes 21°C 7xl0³

60 minutes Yes 30°C < 10³

60 minutes Yes 37°C~ < 10³

60 minutes No 37°C 35xl0⁷

The above results indicate that hydroxymethylglycinate inactivates bacteria, as well as viruses, in blood plasma. The above results also indicate that the inactivation is temperature dependent.

EXAMPLE 8

Four hundred sixty-five microliters of blood plasma and 30 μL of T4 stock were added to each of eight tubes. 5 μL of 50% stock solution of hydroxymethylglycinate, pH 9.0, were added to each of two of the eight tubes. A sample of the aforementioned hydroxymethylglycinate stock solution was adjusted to pH 7.8 with solid sodium phosphate, and 5 μL of the pH-adjusted hydroxymethylglycinate solution were added to another two of the eight tubes. 5 μL of buffer, pH 9.0, were added to another two of the eight tubes, the remaining two tubes serving as controls . The mixtures were incubated at 30^*C. At the times indicated below, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37°C, plaques were counted. The results are summarized below in TABLE VIII.

TABLE VIII

EXAMPLE 9

Each of the hydroxymethyl derivatives listed below was synthesized along the lines described in

U.S. Patent No. 4,337,269 by mixing 10 mmoles of the corresponding L-amino acid with 10 mmoles of 50% aqueous sodium hydroxide and 10 mmoles of 37% formaldehyde. After overnight incubation at room temperature, no free formaldehyde could be detected in any of the reaction mixtures.

465 μL of blood plasma and 30 μL of T4 virus were added to each of nine tubes. In addition, to each tube was added 5 μL of one of the hydroxymethyl derivatives listed below, the remaining tube having no hydroxymethyl derivative and serving as a control The mixtures were incubated at 30^*C for 60 minutes. Samples were then taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE IX.

TABLE IX

INHIBITOR PFU's

Hydroxymethylglycine <10³

Hydroxymethylalanine 14xl0³

Hydroxymethylaspartate 7xl0⁴

Hydroxymethyllysine 28xl0⁴

Hydroxymethylomithine 33x10^s

Hydroxymethylproline 36x10"

Hydroxymethylserine 20xl0³

Hydroxymethylvaline 54X10³

None 6xl0⁷

EXAMPLE 10 10 μL of T4 virus stock and 89 μL of blood plasma were added to each of seven tubes. 1 μL of an appropriate hydroxymethylvaline stock solution was added to each of three of the plasma-containing tubes and 1 μL of an appropriate hydroxymethylaspartate stock solution was added to each of another three of the plasma-containing tubes to give the below-listed concentrations. 1 μL of a buffer solution was added to the remaining tube as a control . After incubation at 30 ^*C for 1 hour, samples from each tube were taken, diluted, mixed with host cells, and overlaid onto solid medium. After overnight incubation at 37°C, plaques were counted. The results are summarized below in TABLE X.

TABLE X

INHIBITOR CONCENTRATION PFU's

Hydroxymethy1valine 0.25% 35xl0⁵ rlydroxymethylaspartate 0.25% 12xl0⁵

Hydroxymethylvaline 0.1% 42xl0⁵ rlydroxymethylaspartate 0.1% 13xl0⁶

Hydroxymethy1valine 0.05% 57xl0⁶ hydroxymethylaspartate 0.05% 20xl0⁶

None 0% 29xl0⁷

EXAMPLE 11 T4 virus stock and blood plasma were added to each of six tubes in the manner described above. Hydroxymethyl-o-phosphorylethanolamine was added to each of two tubes, hydroxymethyl-trp-gly-gly was added to each of another two tubes, and a buffer containing no inhibitor was added to each of the remaining two tubes. The mixtures were incubated at 30^*C. At the times indicated below, samples were taken from each tube, diluted, and plated. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE XI. TABLE XI

Inhibitor Incubation PFU' s Period

Hydroxyme t hy 1 - o - 30 minutes 11x10" phosphorylethanolamine

Hydroxyme thyl - trp - gly- gly 30 minutes 17xl0⁹

None 30 minutes ND

Hydroxyme t hy 1 - o - 60 minutes 40X10³ phosphorylethanolamine

Hydroxymethyl -trp-gly-gly 60 minutes 18xl0⁹

None 60 minutes 25xl0⁹

EXAMPLE 12

465 μL of blood plasma, 30 μL of T4 stock solution were added to each of ten tubes. 5 μL of a 50% solution of hydroxymethylaminopropanol were added to each of .two tubes. 5 μL of a 50% solution of hydroxymethylpenicillinamine were added to each of another two tubes. 5 μL of a 50% solution of hydroxymethylcysteine ethyl ester were added to each of another two tubes. 5 μL of PBS were added to each of another two tubes. 5 μL of a solution containing equimolar amounts of NaOH and formaldehyde incubated overnight at room temperature were added to each of the two remaining tubes. The mixtures were incubated at 30°C. At the times indicated below, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37°C, plaques were counted. The results are summarized below in TABLE XII.

TABLE XII

Inhibitor Incubation PFU' s Period

Hydroxymethylaminopropanol 30 minutes 11x10^s

Hydroxyme thy lpenic i 11 inamine 30 minutes 8x10^s

Hydroxymethylcysteine ethyl 30 minutes 6xl0⁷ ester Inhibitor Incubation PFU's Period

None 30 minutes ND

Alkalinized formaldehyde 30 minutes ND

Hydroxymethylaminopropanol 60 minutes 5x10^s

Hydroxyme hylpenicillinamine 60 minutes 24x10^s

Hydroxymethylcysteine ethyl 60 minutes 6xl0⁷ ester

None 60 minutes 16xl0⁷

Alkalinized formaldehyde 60 minutes 14x10^s

EXAMPLE 13 465 μL of blood plasma, 30 μL of T4 stock solution were added to each of eight tubes. 5 μL of a 50% solution of hydroxymethylcysteine were added to each of two tubes. 5 μL of a 50% solution of hydro¬ xymethyl-aminophenyl acetic acid were added to each of another two tubes. 5 μL of a 50% solution of hydroxymethylaminoethanol were added to each of another two tubes. 5 μL of a buffer solution serving as a control were added to each of the two remaining tubes. The mixtures were incubated at 30^*C. At the times indicated below, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37'C, plaques were counted. The results are summarized below in TABLE XIII.

TABLE XIII

INHIBITOR INCUBATION PERIOD PFU's

Hydroxymethylcysteine 30 minutes 7X10⁷

Hydroxymethyl- 30 minutes 23xl0⁴ aminophenyl acetic acid

Hydroxymethylamino¬ 30 minutes 12x10^s ethanol

None 30 minutes ND

Hydroxymethylcysteine 60 minutes 34x10^s

Hydroxymethyl- 60 minutes 23X10³ aminophenyl acetic acid

Hydroxymeth 1amino- 60 minutes 11x10^s ethanol

None 60 minutes 29X10⁷

EXAMPLE 14

465 μL of blood plasma, 30 μL of T4 stock solution were added to each of eight tubes. 5 μL of a 50% solution of hydroxymethylmercaptopropionylglycine were added to each of two tubes. 5 μL of a 50% solution of hydroxymethylmercaptoethylamine were added to each of another two tubes. 5 μL of a 50% solution of hydroxymethylaminoethyl hydrogen sulfate were added to each of another two tubes. 5 μL of a buffer solution serving as a control were added to each of the two remaining tubes. The mixtures were incubated at 30"C. At the times indicated below, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE XIV. TABLE XIV

INHIBITOR INCUBATION PERIOD PFU's

Hydroxymethylmercapto 30 minutes 15x10^s propionylglycine

Hydroxymethyl- 30 minutes 20xl0⁶ mercaptoethylamine

Hydroxymethylamino- 30 minutes 23x10^s ethyl hydrogen sulfate

None 30 minutes ND

Hydroxymethylmercapto- 60 minutes 36xl0⁵ propionylglycine

Hydroxymethyl- 60 minutes 22x10^s mercaptoethylamine

Hydroxymethylamino¬ CO minutes 20x10^s ethyl hydrogen sulfate

None 60 minutes 17xl0⁷

EXAMPLE 15

The virucidal activities of hydroxymethyl-p- aminohippurate, hydroxymethylpropargyl-glycine, and hydroxymethyl-o-phosphothreonine, respectively, were tested in the manner described above. The results are summarized below in TABLE XV.

TABLE XV

INHIBITOR INCUBATION PERIOD PFU's

Hydroxymethyl-p-amino- 30 minutes <10³ hippurate

Hydroxymethylpropargyl- 30 minutes 10³ glycine

Hydroxymethyl-o- 30 minutes lxlO³ phospho-threonine

None 30 minutes ND

Hydroxymethyl-p-amino- 60 minutes <10³ hippurate

Hydroxymethylpropargyl- 60 minutes <10³ glycine

Hydroxymethyl-o- 60 minutes <10³ phospho-threonine INHIBITOR INCUBATION PERIOD PFU's

None 60 minutes 4xl0⁷

EXAMPLE 16

The virucidal activities of hydroxymethyl- aminoadipate, hydroxymethyl-o-phosphoserine, and hydroxymethylaminoethylphosphonic acid, respectively, were tested in the manner described above. The results are summarized below in TABLE XVI .

TABLE XVI

INHIBITOR INCUBATION PERIOD PFU's

Hydroxymethylamino- 30 minutes 5xl0³ adipate

Hydroxymethyl-o- 30 minutes <10³ phosphoserine

Hydroxymethylamino- 30 minutes 2xl0³ ethylphosphonic acid

None 30 minutes ND

Hydroxymethylamino- 60 minutes 10³ adipate

Hydroxymethyl-o- 60 minutes <10³ phosphoserine

Hydroxymethylamino- 60 minutes <10³ ethylphosphonic acid

None 60 minutes 24xl0⁷

EXAMPLE 17 465 μL of blood plasma and 30 μL of T4 were added to each of six tubes. 5 μL of hydroxymethyl- phosphonomethylglycine were added to each of two tubes, and 5 μL of hydroxymethylmethylhydantion were added to each of another two tubes. 5 μL of buffer were added to each of the remaining two tubes. The mixtures were incubated at 30^*C. At the times indicated below, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE XVII.

TABLE XVII

Inhibitor Incubation PFU's Period

Hydroxymethylphosphono- 30 minutes 28x10" methylglycine

Hydroxymethylmethylhydantion 30 minutes 13x10^s

None 30 minutes ND

Hydroxymethylphosphono- 60 minutes < 10³ methylglycine

Hydroxymethylmethylhydantion 60 minutes 37x10^s

None 60 minutes 25x10^s

EXAMPLE 18

The virucidal activities of hydroxymethylglycine- 7-amido-4-methylcoumarin, hydroxymethyl- vinylglycinate, hydroxymethylfolic acid, hydroxymethyltaurine and hydroxymethyl-aminoethyl trimethyl ammonium chloride, respectively, were tested. in the manner described above at the concentrations indicated below. The results are summarized below in TABLE XVIII. TABLE XVIII

Inhibitor Inhibitor PFU's_* Concentration

Hydroxymethylglycine-7-amido-4- 0.5% 12x10^s methylcoumarin

Hydroxymethyl inylglycinate 0.5% < 10³

Hydroxymethylfolic acid 0.5% < 10³

Hydroxymethyltaurine 0.5% 25X10"

Hydroxymethylaminoethyl trimethyl 0.5% 40x10^s ammonium chloride

Hydroxymethylglycine-7-amido-4- 0.25% 21x10^s methylcoumarin

Hydroxymethylvinylglycinate 0.25% 88X10³

Hydroxymethylfolic acid 0.25% < 10³

Hydroxymethyltaurine 0.25% 22x10^s

Hydroxymethylaminoethyl trimethyl 0.25% 56x10^s ammonium chloride

Hydroxymethylglycine-7-amido-4- 0.1% 19x10^s methylcoumarin

Hydroxymethyl inylglycinate 0.1% 67x10"

Hydroxymethylfolic acid 0.1% 10x10^s

Hydroxymethy11aurine 0.1% 30x10^s

Hydroxymethylaminoethyl trimethyl 0.1% 38x10^s ammonium chloride

None - 15X10⁷

EXAMPLE 19

The virucidal activities of hydroxymethylglycinate, hydroxymethylaminoadipate, hydroxymethylaminoethylphosphonic acid, and hydroxymethyl-o-phosphoserine, respectively, were tested in the manner described above at the con¬ centrations indicated below. The results are summarized below in TABLE XIX. TABLE XIX

Inhibitor Inhibitor PFU'S Concentration

Hydroxymethylglycinate 0.25% 9x10^s

Hydroxymethylaminoadipate 0.25% 14x10^s

Hydroxyme hylaminoethylphosphonic 0.25% 22x10^s acid

Hydroxymethyl-o-phosphoserine 0.25% ^• 32x10^s

Hydroxymethylglycinate 0.1% 16xlO^s_

Hydroxymethylaminoadipate 0.1% 20x10^s

Hydroxymethylaminoethylphosphonic 0.1% 7x10^s acid

Hydroxymethyl-o-phosphoserine 0.1% 14x10^s

Hydroxymethylglycinate 0.05% 2x10^s

Hydroxymethylaminoadipate 0.05% 7xl0⁷

Hydroxymethylaminoethylphosphonic 0.05% 31x10^s acid

Hydroxymethyl-o-phosphoserine 0.05% 20x10^s

None - 5xl0⁷

EXAMPLE 20 The virucidal activities of hydroxymethylphospho- nomethylglycinate and hydroxymethylglycinamide, respectively, were tested in the manner described above at the concentrations indicated below. The results are summarized below in TABLE XX. TABLE XX

Inhibitor Inhibitor PFU's Concentration

Hydroxymethylphosphono- 0.5% ND methylglycinate

Hydroxymethylglycinamide 0.5% 99x10"

Hydroxymethylphosphono- 0.25% 49x10^s methylglycinate

Hydroxymethylglycinamide 0.25% 15x10^s

Hydroxymethylphosphono- 0.1% 34x10^s methylglycinate

Hydroxymethylglycinamide 0.1% 72x10^s

Hydroxymethylphosphono- 0.05% 55x10^s methylglycinate

Hydroxymethylglycinamide 0.05% ND

None - 55xl0⁷

EXAMPLE 21

The virucidal activities of hydroxymethylglycinate, hydroxymethylaminohippurate, hydroxymethylpropargylglycine and hydroxymethyl-O- phosphothreonine, respectively, were tested in the manner described above at the concentrations indi¬ cated below. The results are summarized below in TABLE XXI.

TABLE XXI

Inhibitor Inhibitor PFU's Concentration

Hydroxymethylglycinate 0.25% 15x10"

Hydroxymethylaminohippurate 0.25% 54x10^s

Hydroxymethylpropargylglycine 0.25% 27xl0³

Hydroxymethyl-O-phosphothreonine 0.25% 10X10³

Hydroxymethylglycinate 0.1% 7x10^s

Hydroxymethylaminohippurate 0.1% 45x10^s

Hydroxymethylpropargylglycine 0.1% 41x10^s

Hydroxymethyl-O-phosphothreonine 0.1% 36x10"

Hydroxymethylglycinate 0.05% 43x10^s

Hydroxymethylaminohippurate 0.05% 44x10^s

Hydroxymethylpropargylglycine 0.05% 29x10^s

Hydroxymethyl-O-phosphothreonine 0.05% 15x10^s

None - 32X10⁷

EXAMPLE 22

The virucidal activities of hydroxymethylthreonine, hydroxymethylphos- phothreonine, hydroxymethylserine and hydro- xymethylphosphoserme, respectively, were tested in the manner described above at the concentrations indicated below. The results are summarized below in TABLE XXII.

TABLE XXII

Inhibitor Inhibitor PFU's Concentration

Hydroxymethy1threonine 0.25% 86x10"

Hydroxymethylphosphothreonine 0.25% 75x10"

Hydroxymethylserine 0.25% 14x10^s

Hydroxymethylphosphoserine 0.25% 42x10^s

Hydroxymethylthreonine 0.1% 45x10^s

Hydroxymethylphosphothreonine 0.1% 30X10⁵

Hydroxymethylserine 0.1% 22x10^s

Hydroxymethylphosphoserine 0.1% 21X10^S

Hydroxymethyl hreonine 0.05% 5x10^s

Hydroxymethylphosphothreonine 0.05% 43x10^s

Hydroxymethylserine 0.05% 17X10⁷

Hydroxymethylphosphoserine 0.05% 51x10^s

None - 42X10⁷

EXAMPLE 23

The virucidal activities of hydroxymethyl-MTH- glycine, hydroxymethyl-1-amino-1-cyclopropane carboxylic acid, hydroxymethyl-d, 1-2- aminophosphonopropionic acid, hydroxy-methyl-p- aminobenzoic acid, hydroxymethylamino-butyrolactone, hydroxymethyl-d, 1-aminophosphonobutyric acid, hydroxymethylaminopyrazole carboxylic acid, hydroxymethylazetidine carboxylate and hydroxymethyldiaminobutyric acid, respectively, were tested in the manner described above at the concentrations indicated below. The results are summarized below in TABLE XXIII. TABLE XXIII

Inhibitor Inhibitor PFU's Concentration

Hydroxymethyl-MTH-glycine 0.5% 13x10^s

Hydroxymethyl-1-amino-1- 0.5% 17xl0³ cyclopropane carboxylic acid

Hydroxymethyl-d, 1-2- 0.5% < 10³ aminophosphonopropionic acid

Hydroxymethyl-p-aminobenzoic 0.5% 5x10³ acid

Hydroxymethylaminobutyrolactone 0.5% 30X10³

Hydroxymethyl-d, 1- 0.5% < 10³ aminophosphonobutyric acid

Hydroxymethylaminopyrazole 0.5% 45x10" carboxylic acid

Hydroxymethyl azetidine 0.5% 7x10³ carboxylate

Hydroxymethyldiaminobutyric acid 0.5% < IO³

Hydroxymethyl-MTH-glycine 0.25% 15xl0⁷

Hydroxymethyl-1-amino-1- 0.25% 18x10" cyclopropane carboxylic acid

Hydroxymethyl-d, 1-2- 0.25% 11x10" aminopKosphonopropionic acid

Hydroxymethyl-p-aminobenzoic 0.25% 30x10" acid

Hydroxymethylaminobutyrolactone 0.25% ND

Hydroxymethyl-d, l- 0.25% < 10³ aminophosphonobutyric acid

Hydroxymethylaminopyrazole 0.25% 21x10" carboxylic acid

Hydroxymethyl azetidine 0.25% 16x10" carboxylate

Hydroxymethyldiaminobutyric acid 0.25% 40x10^s

Hydroxymethyl-MTH-glycine 0.1% 18X10⁷

Hydroxymethylglutamate 0.25% 60x10"

Hydroxymethylmethionine 0.25% 68x10^s

Hydroxymethylserine 0.25% 25X10³

Hydroxymethylglutamate 0.1% 12x10^s

Hydroxymethylmethionine 0.1% 28x10^s

Hydroxymethylserine 0.1% 27x10^s Inhibitor Inhibitor PFU's Concentration

Hydroxymethylglutamate 0.05% 9xl0⁷

Hydroxymethylmethionine 0.05% 7X10⁷

Hydroxymethylserine 0.05% 7x10^s

None - 9x10^β

EXAMPLE 24

89 μL of blood plasma and 10 μL of T4 stock solution were added to each of ten tubes. 1 μL of an appropriate hydroxymethylglutamate stock solution was added to each of three tubes to give the concentrations indicated below. 1 μL of an appropriate hydroxymethylmethionine stock solution was added to each of another three tubes to give the concentrations indicated below. 1 μL of an appropriate hydroxymethylserine stock solution was added to each of another three tubes to give the concentrations indicated below. 1 μL of buffer was added to the remaining tube, which served as a control. The mixtures were incubated at 30^*C. After 1 hour, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE XXIV.

TABLE XXIV

Inhibitor Inhibitor PFU's Concentration

Hydroxymethylglutamate 0.25% 60x10"

Hydroxymethylmethionine 0.25% 68x10^s

Hydroxymethylserine 0.25% 25X10³

Hydroxymethylglutamate 0.1% 12x10^s

Hydroxymethylmethionine 0.1% 28x10^s

Hydroxymethylserine 0.1% 27x10^s

Hydroxymethylglutamate 0.05% 9x10⁷

Hydroxymethylmethionine 0.05% 7x10^s

Hydroxymethylserine 0.05% 7x10^s

None - 9xl0⁸

EXAMPLE 25

89 μL of blood plasma and 10 μL of T4 stock solution were added to each of ten tubes. 1 μL of an appropriate hydroxymethylserine stock solution was added to each of three tubes to give the concentrations indicated below. 1 μL of an appropriate hydroxymethyl-0-alanine stock solution was added to each of another three tubes to give the concentrations indicated below. 1 μL of an appropriate hydroxymethylglycine stock solution was added to each of another three tubes to give the concentrations indicated below. 1 μL of buffer was added to the remaining tube, which served as a control. The mixtures were incubated at 30^*C. After 1 hour, samples were taken, diluted, mixed with host cells and overlaid onto solid medium. After overnight incubation at 37^*C, plaques were counted. The results are summarized below in TABLE XXV. TABLE XXV

Inhibitor Inhibitor PFU's Concentration

Hydroxymethylserine 0.25% 8x10"

Hydroxymethyl-β-alanine 0.25% 15x10"

Hydroxymethylglycine 0.25% 9X10³

Hydroxymethylserine 0.1% 14x10^s

Hydroxymethyl-β-alanine 0.1% 33x10^s

Hydroxymethylglycine 0.1% 8x10"

Hydroxymethylserine 0.05% 21X10^S

Hydroxymethyl-β-alanine 0.05% 36x10^s

Hydroxymethylglycine 0.05% 26x10^s

None - 12x10^s

The following is a listing of virucidal activities observed by the present inventors for a variety of hydroxymethyl derivatives (virucidal activity being expressed in terms of PFU's) :

<10³

Hydroxymethylglycine

Hydroxymethylphosphonomethylglycine

Hydroxymethyl-p-aminohippurate

Hydroxymethylpropargylglycine

Hydroxymethyl-o-phosphoserine

Hydroxymethylaminoethyl-phosphonic acid

Hydroxymethylleucine

Hydroxymethyl-β-alanine

Hydroxymethylcysteine

Hydroxymethylphenylalanine 1-2!

Hydroxymethylaminophenylacetic acid

Hydroxymethyl-o-phosphorylethanolamine

Hydroxymethylalanine Hydroxymethylserine

Hydroxymethylvaline

Hydroxymethy1methionine

Hydroxymethylglutamate

IO⁴ Hydroxymethylaspartate

Hydroxymethyllysine Hydroxymethylproline

10⁵ Hydroxymethylmercaptopropionylglycine Hydroxymethylmercaptoethylamine

Hydroxymethylaminoethyl hydrogen sulfate

Hydroxymethy1aminoethano1

Hydroxymethylpenicillamide

Hydroxymethylhydantion Hydroxymethylornithine

Hydroxymethylthreonine

IO⁶

Hydroxymethylcysteine

Hydroxymethy1aminopropano1 10⁷

Hydroxymethyluridine

Hydroxymethylphthalimide

Dimethylurea

5 Hydroxymethylcysteineethyl ester

Hydroxymethy11eucinamide Hydroxymethylarginine Hydroxymethyltyrosine

10. 10 Hydroxymethyldeoxyuridine

4-hydroxymethylimidazole

6-hydroxymethylpterin Hydroxymethylacryl mide Hydroxymethy1cytosine 15 Hydroxymethyl-6-methyluracil

Hydroxymethylnicotinamide

Hydroxymethyl-trp-gly-gly

Hydroxymethylglutamine

Diazolidinylurea

20 Imidazolidinylurea

Kinetic Studies

A kinetic model was developed to monitor the complexation between hydroxymethylglycinate (HMG) and a virus. In the kinetic model it was assumed that the reaction of one molecule of HMG with a virus was sufficient to inactivate the virus. Therefore, the overall reaction can be expressed by the simple rate law:

HMG + V > VHMG

where "V" is a virus. A large excess of HMG was present in the reaction conditions and therefore a pseudo first order equation could be used. Furthermore, it was assumed that in a blood bag, no mass transfer effects exist which would affect the reaction kinetics.

It was found that the rate of inactivation follows the model prediction and that the virus can be inactivated in plasma, whole blood and red blood cell concentrate (RBC) . The rate is also increased as the temperature of the reaction conditions is increased.

Inactivation of SV4-0, Reovirus, Porcine Parvo virus and Polio virus were demonstrated. For SV4-0, Reovirus and Polio virus, the number of viable cells was reduced below detectable limits. Plasma hemoglobin, potassium, sodium, 2,3-DPG, ATP and lactate were unchanged with reference to a control when treated with HMG at a- final concentration of 1600 ppm. The embodiments of the present invention recited herein are intended to be merely exemplary and those skilled in the art will be able to make numerous variations and modifications to it without departing from the spirit of the present invention. All such variations and modifications are intended to be within the scope of the present invention as defined by the claims appended hereto.

Claims

CLAIMSWHAT IS CLAIMED IS:

1. A method of inactivating a microorganism in a biological fluid, comprising contacting said biological fluid with an effective amount of a microorganism-inactivating hydroxymethylamine (HMA) .

2. The method of claim 1 wherein said microorganism is selected from the group consisting of bacteria, viruses, yeasts and molds.

3. The method of claim 2, wherein said microorganism is a bacterium.

4. The method of claim 2, wherein said microorganism is a virus.

5. The method of claim 1, wherein said HMA is a compound of Formula (I)

R

\ N-CH₂-OH (I)

/ R¹

wherein:

R is chosen from the group consisting of hydrogen, alkyl, aryl, substituted alkyl, substituted aryl; R¹ is chosen from the group consisting of acid-, amide-, hydroxy- or mercapto-functional alkyl; acid-, amide-, hydroxy- or mercapto-functional aryl; acid-, amide-, hydroxy- or mercapto- functional substituted alkyl; and acid-, amide-, hydroxy- or mercapto-functional substituted aryl;

or R and R¹ may be joined together to form an acid, amide or hydroxy-functional heterocyclic structure.

6. The method of claim 5, wherein said acid functional group is selected from the group consisting of carboxylate, phosphate, phosphonate, sulfate and sulfonate.

7. The method of claim 6, wherein said acid functional group is a carboxylate.

8. The method of claim 5, wherein said hydroxymethylamine is selected from the group consisting of hydroxymethylglycinamide, hydroxy- methylpenicillinamide, hydroxymethylleucinamide, hydroxymethylacrylamide and hydroxymethyl- nicotinamide.

9. The method of claim 5, wherein said hydroxymethylamine is selected from the group consisting of hydroxymethylglycine, hydroxymethyl- phosphonomethylglycme, hydroxymethyl-p-aminohippuric acid, hydroxymethylpropargylglycine, hydroxymethyl-o- phosphothreonine, hydroxymethylaminoadipic acid, hydroxymethyl-o-phosphoserine, hydroxymethylamino- ethylphosphonic acid, hydroxymethylleucine, hydroxymethyl-β-alanine, hydroxymethylcysteine, hydroxymethylfolic acid, hydroxymethylaminophospheno- butyric acid, hydroxymethylphenylalanine, hydroxymethylaminophenylacetic acid, hydroxymethyl-o- phosphorylethanolamine, hydroxymethylalanine, hydroxymethylserine, hydroxymethylvaline, hydroxymethylmethionine, hydroxymethylglutamic acid, hydroxymethylaspartic acid, hydroxymethyllysine, hydroxymethylproline, hydroxymethylmercaptopropioHyl- glycine, hydroxymethylmercaptoethylamine, hydroxymethylaminoethyl hydrogen sulfate, hydroxymethylaminoethanol, hydroxymethyl- penicillamine, hydroxymethyIhydantoin, hydroxymethylornithine, hydroxymethylcysteine, hydroxymethylaminopropanol, hydroxymethyldiethanol- amine and salts thereof.

10. The method of claim 9, wherein said hydroxymethylamine is hydroxymethylglycine or a salt thereof.

11. The method of claim 1, wherein said hydroxymethylamine and biological fluid are combined to produce a final concentration of hydroxymethylamine in said biological fluid of approximately 0.05 % - 3.0 % by weight.

12. The method of claim 1, wherein said biological fluid and hydroxymethylamine are contacted for a period of time from 0.5 hours to 4 hours.

13. The method of claim 1, wherein said biological fluid is contacted with a hydroxymethylamine at a temperature of between about 4° C and about 30° C.

14. The method of claim 1, wherein said biological fluid is whole blood or blood components.

15. The method of claim 14, wherein said blood components are selected from the group consisting of red blood cells, red blood c.ell concentrate, platelets, platelet concentrate, platelet rich plasma, platelet poor plasma, source plasma

(plasmaphoresis plasma) , fresh frozen plasma and plasma proteins.

16. The method of claim 1, wherein said biological fluid is selected from the group consisting of lymph, cerebrospinal fluid, semen and saliva.

17. A method of processing a biological fluid intended for administration to an individual in need thereof, said method comprising the steps of:

(a) treating the biological fluid with an effective amount of a pathogen-inactivating hydroxymethylamine, thereby producing a treated biological fluid; and

(b) after said treating step, removing free hydroxymethylamine from the treated biological fluid.

18. A method of treating an individual in need of a biological fluid, said method comprising the steps of:

(b) administering the treated biological fluid to the individual in need thereof.

19. A method of treating a biological fluid, said method comprising combining an effective amount of a virus-inactivating hydroxymethylamine with said biological fluid, whereby at least about a 10-fold reduction in plaque forming units of virus is realized.