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PSMF1

From Wikipedia, the free encyclopedia
PSMF1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesPSMF1, PI31, proteasome inhibitor subunit 1
External IDsOMIM: 617858; MGI: 1346072; HomoloGene: 38231; GeneCards: PSMF1; OMA:PSMF1 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_144889
NM_212446
NM_001305244

RefSeq (protein)

NP_001292173
NP_997611

Location (UCSC)Chr 20: 1.11 – 1.19 MbChr 2: 151.56 – 151.59 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Proteasome inhibitor PI31 subunit is a protein that in humans is encoded by the PSMF1 gene.[5][6]

Function

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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMF1 encodes a protein termed PI31 that binds to both 20S and 26S proteasomes. PI31 was originally identified by its ability to inhibit the hydrolysis of small synthetic peptides in a cell free assay, but it stimulates 26S proteasome activity both in vitro and in vivo.[7][8] PI31 can promote the assembly of 26S proteasomes from 19S and 20S sub-particles, and it also serves as an adapter to couple proteasomes with motor proteins to mediate fast axonal transport in neurons.[9] Inactivation of PMSF1 in yeast, plants, fruit flies and mice attenuates protein degradation and stimulates accumulation of ubiquitinated protein aggregates. Furthermore, loss of PSMF1 function in mice causes progressive neuronal dysfunction, synaptic degeneration and eventually neuronal cell death.[10] Alternative transcript variants have been identified for this gene.[6]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000125818Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032869Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Tanahashi N, Kawahara H, Murakami Y, Tanaka K (Apr 1999). "The proteasome-dependent proteolytic system". Molecular Biology Reports. 26 (1–2): 3–9. doi:10.1023/A:1006909522731. PMID 10363639. S2CID 38305744.
  6. ^ a b "Entrez Gene: PSMF1 proteasome (prosome, macropain) inhibitor subunit 1 (PI31)".
  7. ^ Chu-Ping, M (1992). "Purification and characterization of a protein inhibitor of the 20S proteasome (macropain)". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1119 (3): 303–11. doi:10.1016/0167-4838(92)90218-3. PMID 1312359.
  8. ^ Bader, Maya (2011). "A conserved F box regulatory complex controls proteasome activity in Drosophila". Cell. 145 (3): 371–382. doi:10.1016/j.cell.2011.03.021. PMC 3108249. PMID 21529711.
  9. ^ Liu, Kai (2019). "PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development". Dev Cell. 50 (4): 509–524. doi:10.1016/j.devcel.2019.06.009. PMC 6702053. PMID 31327739.
  10. ^ Minis, Adi (2019). "The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance, and neuronal survival in mice". Proc Natl Acad Sci U S A. 116 (49): 24639–24650. Bibcode:2019PNAS..11624639M. doi:10.1073/pnas.1911921116. PMC 6900516. PMID 31754024.

Further reading

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