Myoblast city
| Myoblast city | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | mbc | ||||||
| UniProt | Q9VCH4 | ||||||
| |||||||
Myoblast city (Mbc[1]) is the Drosophila melanogaster ortholog of the mammalian protein Dock180.[2] Mutant mbc embryos exhibit defects in dorsal closure, cytoskeletal organization, myogenesis, and neural development.[2][3]
Discovery
[edit]The Myoblast city locus was identified by deletion mapping, using this technique researchers were able to isolate the location of the gene on the right arm of the third chromosome.[4] During the process four recessive alleles of Mbc were found; mbcc1, mbcc2, mbcc3, mbcs4, all of which are lethal and the D. melanogaster embryos fail to hatch.[4]
During the first 9-10 hours of development, embryos with the mutant Mbc alleles show the same myosin expression as wild-type embryos.[4] However, at about the 11th hour, most myoblasts fail to fuse. As development progresses, some myoblasts show signs of fusion, such as elongation and having multiple nuclei; but some remain round.[4] After 13-14 hours of development, most cells in mutant embryos that failed to fuse lose myosin expression.[4]
References
[edit]- ^ "FlyBase Gene Report:Dmel\mbc". FlyBase Consortium. Retrieved February 25, 2009.
- ^ a b Erickson MR, Galletta BJ, Abmayr SM (August 1997). "Drosophila myoblast city encodes a conserved protein that is essential for myoblast fusion, dorsal closure, and cytoskeletal organization". J. Cell Biol. 138 (3): 589–603. doi:10.1083/jcb.138.3.589. PMC 2141626. PMID 9245788.
- ^ Nolan KM, Barrett K, Lu Y, Hu KQ, Vincent S, Settleman J (November 1998). "Myoblast city, the Drosophila homolog of DOCK180/CED-5, is required in a Rac signaling pathway utilized for multiple developmental processes". Genes Dev. 12 (21): 3337–42. doi:10.1101/gad.12.21.3337. PMC 317223. PMID 9808621.
- ^ a b c d e Rushton, E.; Drysdale, R.; Abmayr, S.M.; Michelson, A.M.; Bate, M. (1995-07-01). "Mutations in a novel gene, myoblast city, provide evidence in support of the founder cell hypothesis for Drosophila muscle development". Development. 121 (7): 1979–1988. doi:10.1242/dev.121.7.1979. ISSN 1477-9129. PMID 7635046.
External links
[edit]- myoblast+city+protein,+Drosophila at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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