An optical microscopy approach with an ultra-large field of view but retained subcellular resolution allows simultaneous imaging of neural activity in widely dispersed brain regions.

A new type of microscope reveals detail of organelles inside every cell in the entirety of an intact embryo or tissue region over 100 cubic millimeters in volume.

Infrared high-pulse-energy low-pulse-repetition-rate excitation advances deep intravital microscopy in strongly scattering tissues such as skin tumors and thick bone, thereby bringing previously inaccessible tumor areas in reach with subcellular resolution.

Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.

An all-optical 3D two-photon imaging and photostimulation platform was demonstrated, with the capability to precisely stimulate a large group of cells in mice cortex in vivo with low laser power.

Calcium imaging reveals spatiotemporal properties of correlated spontaneous activity in the embryonic retina and implicates a role for electrical and chemical synapses in generating the activity.

Using long-term in vivo imaging with miniature two-photon microscopy, microglia surveillance is sleep state-dependent and stress-induced in freely behaving mice, regulated by the locus coeruleus-norepinephrine system and β₂-adrenergic receptors signaling.

In vivo imaging reveals that gradually increased amount of glucose mediates the heterogeneous functional development of individual β-cells by activating its major downstream calcineurin/NFAT signaling pathway.

Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.

A novel single-objective light-sheet microscope with unparalleled spatiotemporal resolution enables imaging and optical manipulation of diverse biological specimens and processes.