MCM5 faktor licenciranja replikacije DNK jest protein koji je kod ljudi kodiran genom MCM5 sa hromosoma 22.[5][6][7]

MCM5
Identifikatori
AliasiMCM5
Vanjski ID-jeviOMIM: 602696 MGI: 103197 HomoloGene: 4904 GeneCards: MCM5
Lokacija gena (čovjek)
Hromosom 22 (čovjek)
Hrom.Hromosom 22 (čovjek)[1]
Hromosom 22 (čovjek)
Genomska lokacija za MCM5
Genomska lokacija za MCM5
Bend22q12.3Početak35,400,134 bp[1]
Kraj35,425,431 bp[1]
Lokacija gena (miš)
Hromosom 8 (miš)
Hrom.Hromosom 8 (miš)[2]
Hromosom 8 (miš)
Genomska lokacija za MCM5
Genomska lokacija za MCM5
Bend8|8 C1Početak75,836,197 bp[2]
Kraj75,855,067 bp[2]
Obrazac RNK ekspresije


Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija nucleotide binding
vezivanje sa DNK
GO:0004003 DNA helicase activity
DNA replication origin binding
chromatin binding
GO:0001948, GO:0016582 vezivanje za proteine
hydrolase activity
ATP binding
GO:0008026 helicase activity
single-stranded DNA binding
GO:0043142 single-stranded DNA helicase activity
GO:0043140 3'-5' DNA helicase activity
Ćelijska komponenta MCM complex
membrana
jedro
nukleoplazma
citoplazma
citosol
Biološki proces G1 faza
Replikacija DNK
ćelijski ciklus
DNA duplex unwinding
DNA replication initiation
G1/S transition of mitotic cell cycle
double-strand break repair via break-induced replication
pre-replicative complex assembly involved in nuclear cell cycle DNA replication
Izvori:Amigo / QuickGO
Ortolozi
VrsteČovjekMiš
Entrez
Ensembl
UniProt
RefSeq (mRNK)

NM_006739

NM_008566
NM_001302540

RefSeq (bjelančevina)

NP_006730

NP_001289469
NP_032592

Lokacija (UCSC)Chr 22: 35.4 – 35.43 MbChr 8: 75.84 – 75.86 Mb
PubMed pretraga[3][4]
Wikipodaci
Pogledaj/uredi – čovjekPogledaj/uredi – miš

Aminokiselinska sekvenca

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Dužina polipeptidnog lanca je 734 aminokiseline, a molekulska težina 82.286 Da.[7]

1020304050
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTG
FTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLE
EAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVK
IPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTD
QAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCD
RYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMK
KAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPI
GVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKM
REDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKG
EDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAV
EGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDS
DRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLD
AALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTK
QKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

Funkcija

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Protein kodiran ovim genom je strukturno vrlo sličan proteinu CDC46 iz S. cerevisiae, uključenom u inicijaciju replikacije DNK. Kodirani protein je član porodice proteina MCM koji se vezuju za hromatin i može komunicirati sa najmanje dva druga člana ove porodice. Kodirani protein se pojačava u tranziciji iz G0 u G1/S fazu ćelijskog ciklusa i može aktivno učestvovati u regulaciji ćelijskog ciklusa.[7]

Interakcije

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Pokazano je da MCM5 ima interakcije sa:

Reference

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000100297 - Ensembl, maj 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000005410 - Ensembl, maj 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Paul R, Hu B, Musahl C, Hameister H, Knippers R (Sep 1996). "Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5". Cytogenet Cell Genet. 73 (4): 317–21. doi:10.1159/000134366. PMID 8751386.
  6. ^ Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (Dec 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. Bibcode:1999Natur.402..489D. doi:10.1038/990031. PMID 10591208.
  7. ^ a b c "Entrez Gene: MCM5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)".
  8. ^ a b c d e f Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
  9. ^ a b c d DaFonseca CJ, Shu F, Zhang JJ (Mar 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. Bibcode:2001PNAS...98.3034D. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
  10. ^ Fujita M, Kiyono T, Hayashi Y, Ishibashi M (Apr 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
  11. ^ Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE (Dec 1998). "Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation". EMBO J. 17 (23): 6963–71. doi:10.1093/emboj/17.23.6963. PMC 1171044. PMID 9843502.

Dopunska literatura

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