CLSPN
Klaspin jest protein koji je kod ljudi kodiran genom CLSPN sa hromosoma 1.[5][6][7]
Amiokiselininska sekvenca
urediDužina polipeptidnog lanca je 1.339 aminokiselina, a molekulska težina 151.094 Da.[8]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MTGEVGSEVH | LEINDPNVIS | QEEADSPSDS | GQGSYETIGP | LSEGDSDEEI | ||||
| FVSKKLKNRK | VLQDSDSETE | DTNASPEKTT | YDSAEEENKE | NLYAGKNTKI | ||||
| KRIYKTVADS | DESYMEKSLY | QENLEAQVKP | CLELSLQSGN | STDFTTDRKS | ||||
| SKKHIHDKEG | TAGKAKVKSK | RRLEKEERKM | EKIRQLKKKE | TKNQEDDVEQ | ||||
| PFNDSGCLLV | DKDLFETGLE | DENNSPLEDE | ESLESIRAAV | KNKVKKHKKK | ||||
| EPSLESGVHS | FEEGSELSKG | TTRKERKAAR | LSKEALKQLH | SETQRLIRES | ||||
| ALNLPYHMPE | NKTIHDFFKR | KPRPTCHGNA | MALLKSSKYQ | SSHHKEIIDT | ||||
| ANTTEMNSDH | HSKGSEQTTG | AENEVETNAL | PVVSKETQII | TGSDESCRKD | ||||
| LVKNEELEIQ | EKQKQSDIRP | SPGDSSVLQQ | ESNFLGNNHS | EECQVGGLVA | ||||
| FEPHALEGEG | PQNPEETDEK | VEEPEQQNKS | SAVGPPEKVR | RFTLDRLKQL | ||||
| GVDVSIKPRL | GADEDSFVIL | EPETNRELEA | LKQRFWKHAN | PAAKPRAGQT | ||||
| VNVNVIVKDM | GTDGKEELKA | DVVPVTLAPK | KLDGASHTKP | GEKLQVLKAK | ||||
| LQEAMKLRRF | EERQKRQALF | KLDNEDGFEE | EEEEEEEMTD | ESEEDGEEKV | ||||
| EKEEKEEELE | EEEEKEEEEE | EEGNQETAEF | LLSSEEIETK | DEKEMDKENN | ||||
| DGSSEIGKAV | GFLSVPKSLS | SDSTLLLFKD | SSSKMGYFPT | EEKSETDENS | ||||
| GKQPSKLDED | DSCSLLTKES | SHNSSFELIG | STIPSYQPCN | RQTGRGTSFF | ||||
| PTAGGFRSPS | PGLFRASLVS | SASKSSGKLS | EPSLPIEDSQ | DLYNASPEPK | ||||
| TLFLGAGDFQ | FCLEDDTQSQ | LLDADGFLNV | RNHRNQYQAL | KPRLPLASMD | ||||
| ENAMDANMDE | LLDLCTGKFT | SQAEKHLPRK | SDKKENMEEL | LNLCSGKFTS | ||||
| QDASTPASSE | LNKQEKESSM | GDPMEEALAL | CSGSFPTDKE | EEDEEEEFGD | ||||
| FRLVSNDNEF | DSDEDEHSDS | GNDLALEDHE | DDDEEELLKR | SEKLKRQMRL | ||||
| RKYLEDEAEV | SGSDVGSEDE | YDGEEIDEYE | EDVIDEVLPS | DEELQSQIKK | ||||
| IHMKTMLDDD | KRQLRLYQER | YLADGDLHSD | GPGRMRKFRW | KNIDDASQMD | ||||
| LFHRDSDDDQ | TEEQLDESEA | RWRKERIERE | QWLRDMAQQG | KITAEEEEEI | ||||
| GEDSQFMILA | KKVTAKALQK | NASRPMVIQE | SKSLLRNPFE | AIRPGSAQQV | ||||
| KTGSLLNQPK | AVLQKLAALS | DHNPSAPRNS | RNFVFHTLSP | VKAEAAKESS | ||||
| KSQVKKRGPS | FMTSPSPKHL | KTDDSTSGLT | RSIFKYLES |
Funkcija
urediKlaspin u žaba roda Xenopus je esencijalni uzvodni regulator kinazne kontrolne tačke 1 i pokreće zaustavljanje kontrolne tačke ćelijskog ciklusa u prisustvu DNK šablona u ekstraktima ksenopusovih jaja. Čini se da je ljudski gen homolog njegovom klaspinu, a njegova funkcija još nije utvrđena.[7]
Interakcije
urediPokazalo se da CLSPN ima interakcije sa:
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000092853 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000042489 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Kumagai A, Dunphy WG (Nov 2000). "Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts". Mol Cell. 6 (4): 839–49. doi:10.1016/S1097-2765(05)00092-4. PMID 11090622.
- ^ a b Chini CC, Chen J (Aug 2003). "Human claspin is required for replication checkpoint control". J Biol Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.
- ^ a b "Entrez Gene: CLSPN claspin homolog (Xenopus laevis)".
- ^ "UniProt, Q9HAW4" (jezik: engleski). Pristupljeno 23. 11. 2021.
- ^ Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi:10.1073/pnas.0401847101. PMC 404071. PMID 15096610.
- ^ a b c Serçin O, Kemp MG (maj 2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC 3127160. PMID 21478680.
- ^ Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N (2009). "USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin". J. Cell Biol. 184 (1): 13–9. doi:10.1083/jcb.200807137. PMC 2615094. PMID 19124652.
Dopunska literatura
uredi- Chini CC, Chen J (2005). "Claspin, a regulator of Chk1 in DNA replication stress pathway". DNA Repair (Amst.). 3 (8–9): 1033–7. doi:10.1016/j.dnarep.2004.03.001. PMID 15279790.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Lin SY, Li K, Stewart GS, Elledge SJ (2004). "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. Bibcode:2004PNAS..101.6484L. doi:10.1073/pnas.0401847101. PMC 404071. PMID 15096610.
- Sørensen CS, Syljuåsen RG, Lukas J, Bartek J (2006). "ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage". Cell Cycle. 3 (7): 941–5. doi:10.4161/cc.3.7.972. PMID 15190204.
- Sar F, Lindsey-Boltz LA, Subramanian D, et al. (2004). "Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures". J. Biol. Chem. 279 (38): 39289–95. doi:10.1074/jbc.M405793200. PMID 15226314.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Clarke CA, Clarke PR (2005). "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system". Biochem. J. 388 (Pt 2): 705–12. doi:10.1042/BJ20041966. PMC 1138979. PMID 15707391.
- Clarke CA, Bennett LN, Clarke PR (2005). "Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway". J. Biol. Chem. 280 (42): 35337–45. doi:10.1074/jbc.M506460200. PMID 16123041.
- Chini CC, Wood J, Chen J (2006). "Chk1 is required to maintain claspin stability". Oncogene. 25 (30): 4165–71. doi:10.1038/sj.onc.1209447. PMID 16501606.
- Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
- Bennett LN, Clarke PR (2006). "Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation". FEBS Lett. 580 (17): 4176–81. doi:10.1016/j.febslet.2006.06.071. PMID 16828751.
- Liu S, Bekker-Jensen S, Mailand N, et al. (2006). "Claspin operates downstream of TopBP1 to direct ATR signaling towards Chk1 activation". Mol. Cell. Biol. 26 (16): 6056–64. doi:10.1128/MCB.00492-06. PMC 1592810. PMID 16880517.
- Mailand N, Bekker-Jensen S, Bartek J, Lukas J (2006). "Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress". Mol. Cell. 23 (3): 307–18. doi:10.1016/j.molcel.2006.06.016. PMID 16885021.
- Peschiaroli A, Dorrello NV, Guardavaccaro D, et al. (2006). "SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response". Mol. Cell. 23 (3): 319–29. doi:10.1016/j.molcel.2006.06.013. PMID 16885022.
- Mamely I, van Vugt MA, Smits VA, et al. (2006). "Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery". Curr. Biol. 16 (19): 1950–5. doi:10.1016/j.cub.2006.08.026. PMID 16934469.
- Rodríguez-Bravo V, Guaita-Esteruelas S, Florensa R, et al. (2007). "Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells". Cancer Res. 66 (17): 8672–9. doi:10.1158/0008-5472.CAN-05-4443. PMID 16951182.
- Chini CC, Chen J (2006). "Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function". J. Biol. Chem. 281 (44): 33276–82. doi:10.1074/jbc.M604373200. PMID 16963448.
- Sercin O, Kemp MG (2011). "Characterization of functional domains in human Claspin". Cell Cycle. 10 (10): 1599–606. doi:10.4161/cc.10.10.15562. PMC 3127160. PMID 21478680.
Vanjski linkovi
uredi- Lokacija ljudskog genoma CLSPN i stranica sa detaljima o genu CLSPN u UCSC Genome Browseru.