Taq DNA Polymerase: The Enzyme of Choice for Robust and Reliable PCR Amplification  

Introducing Recombinant Taq DNA Polymerase: A Highly Thermostable Enzyme for Robust PCR Amplification

Taq DNA Polymerase is a thermostable (heat-resistant) DNA polymerase enzyme derived from the thermophilic bacterium Thermus aquaticus, known for its ability to thrive in high-temperature environments. This enzyme is widely employed in polymerase chain reaction (PCR), a fundamental technique in molecular biology used to amplify specific DNA fragments. Taq DNA Polymerase exhibits exceptional processivity, fidelity, and thermostability, making it a cornerstone of PCR applications.

Key Features:

• Superior Thermostability: Taq DNA Polymerase retains its enzymatic activity at temperatures up to 95°C, ensuring stability throughout the PCR cycling process.
• High Processivity: Taq DNA Polymerase maintains a strong association with the DNA template, enabling the synthesis of long DNA strands with minimal dissociation.
• Exceptional Fidelity: Taq DNA Polymerase incorporates nucleotides with high accuracy, minimizing errors during PCR amplification and producing high-quality DNA products.

Diverse Applications:

• Amplification of DNA fragments for various molecular biology applications
• DNA sequencing for gene analysis and mutation detection
• Gene mapping to identify the chromosomal location of specific genes
• Forensic analysis for DNA profiling and evidence identification

Unparalleled Benefits:

• Efficient and reliable PCR amplification, even with complex or challenging templates
• Generation of high-quality, error-free DNA products suitable for downstream applications
• Reduced risk of PCR artifacts and misamplifications
• Versatility in a wide range of molecular biology and research applications

Recombinant Taq DNA Polymerase: The Enzyme of Choice for High-Performance PCR

Recombinant Taq DNA Polymerase, produced through advanced recombinant DNA technology, embodies the same exceptional properties as its natural counterpart. This enzyme undergoes rigorous purification steps to ensure the highest quality and consistency, making it the preferred choice for researchers demanding robust and reliable PCR amplification.

Product Composition:

• Taq DNA Polymerase (5 U/μL)
• 10× PCR Buffer (Mg2+-free)
• 25 mM MgCl2

Features and Applications:

• Possesses both 5'-3' polymerase and 5'-3' exonuclease activity, but lacks 3'-5' exonuclease activity (proofreading function)
• Exhibits high extension speed (1-2 kb/min) at optimal temperatures (70-75°C)
• Produces PCR products with A overhangs at the 3' end
• Suitable for a variety of PCR applications, including:
  • General PCR amplification
  • RT-PCR qualitative and quantitative detection
  • Target gene amplification directly linked to T-Vector
  • Fluorescence quantitative PCR detection
  • DNA sequencing

Recombinant Taq DNA Polymerase stands as an indispensable tool for researchers in molecular biology, genetics, and various scientific disciplines. Its exceptional performance and versatility make it the gold standard enzyme for PCR amplification, empowering scientists to unravel the complexities of DNA and advance scientific discoveries.

Taq DNA Polymerase: A Thermostable Enzyme Revolutionizing Molecular Biology

Taq DNA Polymerase, an enzyme derived from the thermophilic bacterium Thermus aquaticus, has revolutionized the field of molecular biology. Its remarkable thermostability, allowing it to function at high temperatures, has made it an indispensable tool for polymerase chain reaction (PCR), a fundamental technique used to amplify DNA fragments.

Key Characteristics:

• Thermostability: Taq DNA Polymerase retains its enzymatic activity at temperatures up to 95°C, enabling it to withstand the repeated cycles of denaturation (95°C) and annealing (50-60°C) employed in PCR.
• Processivity: Taq DNA Polymerase maintains a strong association with the DNA template, enabling the continuous synthesis of long DNA strands without frequent dissociation.
• Fidelity: Taq DNA Polymerase exhibits high accuracy in nucleotide incorporation, minimizing errors during PCR amplification and producing high-quality DNA products.

Diverse Applications:

• PCR Amplification: Taq DNA Polymerase is the cornerstone of PCR, enabling the amplification of specific DNA fragments of interest from various sources.
• DNA Sequencing: Taq DNA Polymerase plays a crucial role in DNA sequencing methods, facilitating the synthesis of complementary DNA strands for Sanger sequencing.
• Mutation Detection: Taq DNA Polymerase is employed in techniques like PCR-based mutagenesis to introduce specific mutations into DNA sequences.
• Gene Mapping: Taq DNA Polymerase is utilized in gene mapping strategies to determine the chromosomal location of specific genes.
• Forensic Analysis: Taq DNA Polymerase is instrumental in forensic DNA profiling, enabling the identification and comparison of DNA samples from crime scenes or individuals.

Unparalleled Benefits:

• Efficient and Reliable PCR Amplification: Taq DNA Polymerase ensures efficient and reliable PCR amplification, even with complex or challenging templates.
• Generation of High-Quality DNA Products: Taq DNA Polymerase produces high-quality, error-free DNA products suitable for downstream applications like cloning, sequencing, and analysis.
• Reduced Risk of PCR Artifacts and Misamplifications: Taq DNA Polymerase's high fidelity minimizes the risk of PCR artifacts and misamplifications, ensuring the integrity and accuracy of amplified DNA.
• Versatility in a Wide Range of Molecular Biology and Research Applications: Taq DNA Polymerase's versatility makes it an indispensable tool in a wide range of molecular biology and research applications.