You can use os.access(path, mode) to check the file permissions and existence with modes for reading, writing and execution permissions.
For example
>>> import os >>> os.access('my_file', os.F_OK) # Check for existence of file True >>> os.access('my_file', os.R_OK) # Check for read access True >>> os.access('my_file', os.W_OK) # Check for write access True >>> os.access('my_file', os.X_OK) # Check for execution access False