Extraction and estimation of Alginate For extraction of
exopolysaccharide, 1 mL of (0.5M) EDTA sodium salt solution and 0.5 mL of (5.0M) NaCl solution was added to the fermentation media.
Treatment of biofilm-associated persistent infections is an emerging issue for clinicians as bacterial cells adhere with human epithelial cells or indwelling medical devices such as implants and catheters used in urinary tract and respiratory infections.1 Biofilms are complex surface-associated communities where bacterial cells are enclosed by self-produced extra cellular polymeric substances (EPS), which mainly consist of
exopolysaccharides, proteins and extracellular deoxyribonucleic acid (DNA).2-4 Pseudomonas (P.) aeruginosa is an important human pathogen which causes many infections ranging from wound infections to cystic fibrosis.
The
exopolysaccharide is produced by microalgae of the genus Parachlorella.
A cellulosic
exopolysaccharide produced from sugarcane molasses by a Zoogloea sp.
It is the matrix that forms biofilms, which components include bacterial cells,
exopolysaccharides, proteins, nucleic acids, glycoproteins, phospholipids, debris, and inorganic matter (SUTHERLAND, 2001).
Tian et al., "Molecular characteristics of an
exopolysaccharide from Lactobacillus rhamnosus KF5 in solution," International Journal of Biological Macromolecules, vol.
Several studies have suggested the organism's higher affinity for endocardium due to the production of a significant amount of
exopolysaccharide and propensity to bind with fibronectin in the extracellular matrix, which further expands their virulence [4, 6].
aureus were established and then exposed to different bacteriophage-derived proteins that catalyze degradation of the key biofilm extracellular polymers peptidoglycan or
exopolysaccharide.
These bacteria encase themselves in a proteinaceous matrix called
exopolysaccharide (EPS) material.