MIA’S Academy

PSC LIFE Academy

168/2016: Questions and

Answers
Lab Technician Grade II
Complete Question and Answer Guide with
Explanations
Q 1. Which of the following has a laterally compressed
body and no wings?

▪ Answer: (C) Fleas

▪
Explanation: Fleas have laterally compressed bodies
that help them move easily through the fur of their
hosts. They are wingless insects adapted for jumping.
2. Cercariae appear at which stage in
the following?

▪ Answer: (B) Schistosoma

▪
Explanation: Cercariae are the larval stage of
Schistosoma, a parasitic flatworm responsible for
schistosomiasis.
3. Nematodes are best fixed in:

▪ Answer: (A) 10% formalin

▪
Explanation: Formalin (10%) is commonly used for
preserving nematodes because it maintains their
structural integrity for microscopic examination.
4. The Clonorchis test is carried out
on:

▪ Answer: (C) Blood

▪
Explanation: Clonorchis is a liver fluke, and serological
tests using blood samples help in detecting its
presence.
5. Commercially available concentrated
formaldehyde is:

▪ Answer: (A) 37–41% weight/volume (w/v)

▪
Explanation: Formaldehyde solutions are typically sold
at this concentration for laboratory use.
Man, fish, snail, and metacercaria are
seen in the life cycle of:

▪ Answer: (B) Trematodes

▪
Explanation: Trematodes (flukes) have a complex life
cycle involving multiple hosts, including fish, snails, and
humans.
7. The eggs of which parasite have a
napoleonic hat shape?

▪ Answer: (D) Schistosoma spindale

▪
Explanation: Schistosoma spindale eggs have a
characteristic lateral spine, giving them a hat-like
appearance.
8. For routine staining of blood smears by
Giemsa’s method, which sample is used?

▪ Answer: (C) Blood

▪
Explanation: Giemsa stain is commonly used to stain
blood smears to detect parasites like Plasmodium.
9. The etiological agent of Anthrax is:

▪ Answer: (A) Bacillus anthracis

▪
Explanation: Bacillus anthracis is a gram-positive,
spore-forming bacterium responsible for anthrax.
10. In Dal’s samples, nits refer to:

▪ Answer: (C) Lice eggs

▪
Explanation: Nits are the eggs of lice, often found
attached to hair shafts.
11. Nit is the first stage in the life
cycle of:

▪ Answer: (C) Lice

▪
Explanation: Nits are lice eggs, which represent the
first stage of their life cycle.
12. The egg with an operculum and flat
shape belongs to:

▪ Answer: (A) Fasciola hepatica

▪
Explanation: Fasciola hepatica eggs are operculated
and oval, typical of liver flukes.
13. The solution used to tolerate
nematodes for histopathology is:

▪ Answer: (C) Neutral buffered formaldehyde

▪
Explanation: This solution preserves nematodes while
maintaining tissue structure for microscopic
examination.
14. The parasite that adheres to
regulate light and enzyme production is:

▪ Answer: (B) Giardia

▪
Explanation: Giardia is a flagellated protozoan that
regulates enzyme production to survive in the host.
15. A fixative containing picric acid,
formaldehyde, and glacial acetic acid is:

▪ Answer: (A) Bouin’s solution

▪
Explanation: Bouin’s solution is used in histology to
preserve tissue morphology
16. For fixation, the recommended
quantity of fixative should be

▪ AnsAnswer: (A) 20 times the tissue volume

▪
Explanation: This ensures proper tissue penetration
and preservation.
17. The unit for expressing Erythrocyte
Sedimentation Rate (ESR) is:

▪ Answer: (B) mm/hour

▪
Explanation: ESR is measured in millimeters per hour,
indicating the rate at which red blood cells settle in a
test tube
18. The old name for Aspartate
Aminotransferase (AST) is:

▪ Answer: (B) Serum glutamic-oxaloacetic transaminase

(SGOT)
▪
Explanation: AST was historically called SGOT in
clinical practice.
19. Which is not a liver function test?

▪ Answer: (B) Test for serum creatinine

▪
Explanation: Serum creatinine is a kidney function
test, not related to liver function.
20. An example of a special stain is:

▪ Answer: (A) Periodic Acid-Schiff (PAS) stain

▪
Explanation: PAS stain is used to highlight glycogen
and other polysaccharides in tissues.
Q# Question Answer Explanation

Prussian blue is used to detect iron

The staining technique for (A) Prussian blue
21 deposits such as hemosiderin in
intracellular hemosiderin is: stain
tissues.

The sterilization condition in a (A) 121°C, 15 psi This condition is standard for
22
steam jacketed autoclave is: for 15 min sterilization in autoclaves.

The microscope used for Dark-field microscopy is used for

(A) Dark-field
23 detecting leptospires in blood visualizing spirochetes like
microscope
samples is: Leptospira.

This concentration provides the right

The agar incorporation consistency for solidifying the
24 (B) 1.5–2%
percentage for bacterial media is: medium while allowing bacterial
growth.

(A) Fluid
An example of an anaerobic Fluid thioglycollate supports the
25 thioglycollate
medium is: growth of anaerobic organisms.
medium
 Question Answer Explanation
Weigert’s modification is not
Which of the following is not a
part of Gram staining; it is
26 modification of Gram’s (C) Weigert’s modification
used for connective tissue
staining?
staining.
In Lactophenol Cotton Blue Cotton blue in lactophenol
27 stain, the function of cotton (B) Stains fungal structures solution stains fungal elements
blue is: for microscopy.

Heat fixing kills bacteria and

The best method for fixing a adheres them to the slide
28 (B) Heat fixing
bacterial smear is: without altering the cell
structure.

Innate immunity is the natural,

Natural immunity present in an
29 (B) Innate immunity non-specific defense
individual is:
mechanism present from birth.

Nosocomial infections, also

Nosocomial infections are called hospital-acquired
30 (B) Hospital environments
acquired from: infections, are contracted in
healthcare settings.
 Question Answer Explanation

Rabies diagnosis is often

The specimen taken for confirmed through
31 (A) Brain tissue
diagnosing rabies is: examination of brain tissue
postmortem.
Serum is obtained by
For serum separation, blood (D) Plain tubes without allowing blood to clot in
32
is collected in: anticoagulants plain tubes before
centrifugation.
Bacterial colonies with a Umbonate colonies have a
33 raised or convex center are (A) Umbonate colonies raised center, characteristic
called: of certain bacterial species.
It indicates strong acid
The Methyl Red test is used (A) Acid production from
34 production by glucose
to detect: glucose fermentation
fermentation.
Taq polymerase is a heat-
Q# Question Answer Explanation

BIS is the modern name for

Initial Standards Institution is now (D) BIS (Bureau of
36 India’s standards
known as: Indian Standards)
organization.

The maximum permissible level Iodine levels in drinking water

37 of iodine in drinking water in India (C) 134 mcg/L are regulated to prevent
is: deficiencies and toxicities.

The Food and Agriculture

The headquarters of FAO is
38 (B) Rome, Italy Organization (FAO) is based in
located in:
Rome.

Mueller-Hinton agar is the

The medium used for antibiotic (D) Mueller-Hinton standard medium for
39
sensitivity testing is: agar antibiotic susceptibility
testing.
Clot on boiling is a
Which of the following is not a (D) Clot on boiling
40 confirmatory test, not a
platform test for milk? test
platform test.
Q# Question Answer Explanation
Triple phosphate crystals appear
Urine crystals with a coffin-lid (B) Triple
41 like coffin lids and are commonly
appearance are: phosphate
found in alkaline urine.

(B) Serum
glutamic- AST was historically called SGOT,
The old name for Aspartate
42 oxaloacetic commonly used in liver function
Aminotransferase (AST) is:
transaminase tests.
(SGOT) Tellurite blood agar is used for
The selective medium for (A) Tellurite
43 isolating Corynebacterium
Corynebacterium diphtheriae is: blood agar
diphtheriae.
(B) Lactophenol cotton blue highlights
The stain for detecting fungal
44 Lactophenol fungal structures for microscopic
elements is:
cotton blue examination.

(C)
Lowenstein-Jensen medium is the
The recommended medium for Lowenstein-
45 standard medium for growing
Mycobacterium tuberculosis is: Jensen
Mycobacterium species.
medium
Q# Question Answer Explanation

In obstructive jaundice, bile flow is

Urobilinogen is completely (A) Obstructive blocked, preventing urobilinogen from
46
absent in: jaundice reaching the intestines and
bloodstream.

Disseminated intravascular (A) Acute DIC can occur in conditions like

47 coagulation (DIC) is a promyelocytic leukemia, causing excessive clotting
complication of: leukemia and bleeding.

Black urine in phenylketonuria results

Black-colored urine is a (A)
48 from the buildup of phenylalanine and
feature of: Phenylketonuria
its metabolites.

(A) Glucose-6-
phosphate
Heinz bodies are associated Heinz bodies are denatured hemoglobin
49 dehydrogenase
with: seen in G6PD deficiency.
(G6PD)
deficiency
The main apoprotein present Apo B-100 is essential for LDL
50 (C) Apo B-100
Q# Question Answer Explanation

Leptocytes (target cells) are seen (A) Liver Target cells are commonly found in liver
51
in: disease disorders and hemoglobinopathies.

(A) Fibers in
Reticulin stains highlight reticular fibers in
52 Reticulin stain is used to detect: connective
tissues.
tissue

Tellurite blood agar selectively grows

The selective medium for (A) Tellurite
53 Corynebacterium diphtheriae, inhibiting
Corynebacterium diphtheriae is: blood agar
other bacteria.

(A) Acid
It confirms the presence of strong acid-
54 The methyl red test detects: production
producing bacteria like E. coli.
from glucose

The main enzyme involved in (B) Hexokinase phosphorylates glucose in the

55
glycolysis is: Hexokinase first step of glycolysis.
Q# Question Answer Explanation

Western blot is a technique used

Western blot is used to (A)
56 to detect specific proteins in a
detect: Proteins
sample using antibodies.

The coenzyme (C)

PLP, the active form of vitamin
involved in Pyridoxal
57 B6, plays a key role in amino
transamination phosphate
acid metabolism.
reactions is: (PLP)
Taq polymerase is a heat-stable
The enzyme commonly
(B) Taq enzyme that synthesizes DNA
58 used in PCR reactions
polymerase during the polymerase chain
is:
reaction (PCR).

In obstructive jaundice, bile

(A)
Urobilinogen is flow is blocked, preventing
59 Obstructive
completely absent in: urobilinogen from entering the
jaundice
intestines and bloodstream.

Apo B-100 is essential for LDL

The main apoprotein
60 (C) Apo B-100 metabolism and receptor
present in LDL is:
binding.
Q# Question Answer Explanation

The Western blot test is used to Western blotting is a technique used for
61 (A) Proteins
detect: identifying specific proteins in a sample.

Lowenstein-Jensen medium is the

The recommended medium for (C) Lowenstein-
62 standard medium for growing
Mycobacterium tuberculosis is: Jensen medium
Mycobacterium species.

The Gram-negative bacterium Mycoplasma lacks a cell wall, making it

63 (A) Mycoplasma
without a cell wall is: resistant to beta-lactam antibiotics.

The stain for detecting fungal (B) Lactophenol This stain highlights fungal structures for
64
elements is: cotton blue microscopic examination.

The coenzyme involved in (C) Pyridoxal PLP is the active form of vitamin B6,
65
transamination reactions is: phosphate (PLP) essential for amino acid metabolism.
Q# Question Answer Explanation

Xylose Lysine Deoxycholate

The selective medium for
(XLD) agar selectively isolates
66 Salmonella and Shigella (B) XLD agar
Salmonella and Shigella from
is:
other enteric bacteria.
(A) Acid The methyl red test identifies
67 Methyl red test detects: production strong acid production during
from glucose glucose fermentation.
Mycoplasma lacks a cell wall,
Gram-negative bacteria (A)
68 making it resistant to beta-
lacking a cell wall are: Mycoplasma
lactam antibiotics.
Catalase converts hydrogen
The enzyme responsible
peroxide into water and oxygen,
69 for breaking down (B) Catalase
protecting cells from oxidative
hydrogen peroxide is:
damage.
Ciprofloxacin inhibits DNA
Antibiotic acting on DNA (A)
70 gyrase, preventing bacterial
gyrase is: Ciprofloxacin
DNA replication.
Q# Question Answer Explanation

Ciprofloxacin inhibits DNA gyrase,

71 Antibiotic acting on DNA gyrase is: (A) Ciprofloxacin
preventing bacterial replication.

This E. coli strain is the standard

The control strain for antibiotic (B) ATCC 25922
72 control for antibiotic susceptibility
sensitivity testing is: (E. coli)
testing.

(C) Below
Periodic acid is sensitive to light and
73 Periodic acid should be stored: ambient
heat, requiring cool storage.
temperature

Tellurite blood agar is used for

The selective medium for (A) Tellurite
74 isolating Corynebacterium
Corynebacterium diphtheriae is: blood agar
diphtheriae.

(A) Counterstain
It provides a contrasting color to acid-
75 Methylene blue is used as a: in acid-fast
fast organisms.
staining
Q# Question Answer Explanation

The H antigen forms the base

The main antigen in ABO blood (A) H
76 structure for A and B antigens
grouping is: antigen
in the ABO blood group system.

(A) Fibers
Reticulin stains highlight
in
77 Reticulin stain is used to detect: reticular fibers in connective
connective
tissues.
tissue
(A) Prussian blue stain is used to
The stain for intracellular hemosiderin
78 Prussian detect iron deposits such as
is:
blue hemosiderin in tissues.

Dark-field microscopy is
The microscope used for detecting (A) Dark-field
79 used for visualizing
leptospires in blood samples is: microscope
spirochetes like Leptospira.

Tellurite blood agar is used

The selective medium for (A) Tellurite for isolating
80
Corynebacterium diphtheriae is: blood agar Corynebacterium
Q# Question Answer Explanation

Rani Chennamma of Kittur led

Who led the anti-British struggle in (A) Rani
81 a rebellion against British rule
Kittur? Chennamma
in the early 19th century.

Mughal architecture combines

The Mughal architecture is a blending (B) Persian and
82 elements from Persian, Indian,
of: Indian styles
and Islamic styles.

Narayana Guru was a social

(A) Narayana
83 The author of Moksha Pradeepan is: reformer and philosopher from
Guru
Kerala.

Justice Fathima Beevi was the

The first woman judge in the (B) Justice
84 first woman judge in the
Supreme Court of India was: Fathima Beevi
Supreme Court of India.

The Food and Agriculture

The headquarters of FAO is located
85 (B) Rome, Italy Organization (FAO) is based in
in:
Rome.
Q# Question Answer Explanation

Bhadrabahu was a
The author of Jain monk and the
86 (A) Bhadrabahu
Kalpasutra author of the Jain
text Kalpasutra.
Purushapura
The capital of (present-day
(C) Purushapura
87 Kanishka’s empire Peshawar) was the
(Peshawar)
was: capital of the Kushan
emperor Kanishka.
Simhavishnu was the
The founder of the founder of the
88 (B) Simhavishnu
Pallava dynasty is: Pallava dynasty in
South India.
A. K. Gopalan was a
The Malayali political prominent
leader associated communist leader
89 (D) A. K. Gopalan
with the Indian from Kerala and a
Parliament was: member of the Indian
Parliament.
Justice Fathima Beevi
The first woman was the first woman
(B) Justice
90 judge of the Supreme judge to be appointed
Fathima Beevi
Court of India was: to the Supreme Court
Q# Question Answer Explanation
Afonso de
Albuquerque, the
Albuquerque captured Portuguese general,
91 (A) Bijapur
Goa from the ruler of: captured Goa from the
Sultan of Bijapur in
1510.
Rabindranath Tagore
Who described the Taj
(A) Rabindranath poetically described
92 Mahal as “a teardrop
Tagore the Taj Mahal with this
on the cheek of time”?
The Governor of Kerala phrase.
B. R. Bhagat was the
when it came under Governor of Kerala
93 (D) B. R. Bhagat
President’s Rule for the when it first came
first time was: under President’s Rule.
Sri Narayana Guru
Who coined the term used the title Kerala
(B) Sri Narayana
94 Kerala Simham (Lion of Simham for strong
Guru
Kerala)? leaders in Kerala’s
history.
The founder of Sree SNDP was founded by
Narayana Dharma (A) Sri Narayana Sri Narayana Guru to
95
Paripalana Yogam Guru promote social reform
(SNDP) was: and equality.
Q# Question Answer Explanation

The first general The first general

election after the election in Kerala
96 (C) February 1957
formation of Kerala was held in
state was held in: February 1957.
The Chola temples
The oldest example represent the
of the Dravidian earliest and most
97 (B) Chola temples
style of architecture prominent examples
is found in: of Dravidian
architecture.
Nitish Kumar is the
The Chief Minister of
98 (D) Nitish Kumar Chief Minister of
Bihar is:
Bihar.
Chandigarh serves
The headquarters of
as the headquarters
the Western
99 (C) Chandigarh for the Western
Command of the
Command of the
Indian Army is in:
Indian Army.
K. T. Jaleel was the
The Kerala Minister Kerala Minister for
100 for Welfare Affairs (A) K. T. Jaleel Welfare Affairs at
is: the time of this
examination.
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