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Hanging Drop

The document outlines the procedure for demonstrating bacterial motility using the Hanging Drop method, detailing steps for preparation and observation under a microscope. It also describes different arrangements of flagella in bacteria, including peritrichous, polar, monotrichous, lophotrichous, and amphitrichous configurations. Additionally, it defines bacterial motility as a purposeful and directional movement from one location to another.

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microbiology
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13 views10 pages

Hanging Drop

The document outlines the procedure for demonstrating bacterial motility using the Hanging Drop method, detailing steps for preparation and observation under a microscope. It also describes different arrangements of flagella in bacteria, including peritrichous, polar, monotrichous, lophotrichous, and amphitrichous configurations. Additionally, it defines bacterial motility as a purposeful and directional movement from one location to another.

Uploaded by

microbiology
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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monstration of Motility by Hanging Drop Metho

Procedure for Hanging Drop


Preparation:

1.Take a clean cover slip apply a


minimal amount of Vaseline to the
four corners with a thin glass rod, or
an applicator stick.

2. Using a 2mm loop place a loopful of


the given suspension at the centre of
the cover slip.
3. Invert a cavity slide over the cover
slip so that the drop of suspension is
4. Quickly and carefully turn the slide
over so that the coverslip is
uppermost with the drop hanging
from the same into the cavity

5. Focus the edge of the drop under


low power of the microscope after
reducing the light using the
condenser.

6. Turn the high power objective ,


identify the edge and carefully focus
the edge with fine adjustment.
Describe the arrangement of Flagella found among Ba

lagella may be present. If present it may be as follo


a. Peritrichous Arranged all
flagella around the cell
eg Salmonella
b. Polar Arranged at one
or both ends of
the cell.
c. Monotrichous Single flagellum at
one end eg- Vibrio
cholera.
d. Lophotrichous Tuft of Flagella at
one end
e. Amphitrichous Flagella at both
ends
Define motility of Bacteria

Motility is a purposeful and directional movement


of bacteria from one place to another with respect
o one an other

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