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PCR 2009

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13 views11 pages

PCR 2009

Uploaded by

vd.tanna101
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPT, PDF, TXT or read online on Scribd
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Polymerase Chain

Reaction
 The polymerase chain reaction (PCR)
is a technique to amplify a piece of
DNA very rapidly outside of a cell.
Process

- Buffer, polymerase Enzyme, oligonucleotide primers,


nucleotides,

 Denaturation temperature:
94°C

 Annealing temperature
54°C

 Extension temperature
72°C
Process
 25-30 cycles

2 minute cycles

 DNA thermal cycler


Collecting DNA evidence
 Blood  Old samples

 Semen  Small samples

 Tissue  Degenerated
samples
 Saliva
– Envelope, stamps,
cigarette butts
Advantages
 Quick results

 Small amount

 More stable
PCR Uses
 Diagnose genetic
diseases

 Find bacteria and


viruses

 Study human evolution

 Clone DNA of Egyptian


mummies

 Do DNA fingerprinting
Forensic Uses
 Can apply any biological technique

 Identify victim/suspect

 Paternity/maternity tests
Future Uses
 DNA as “id card”

 More studies
References
 Criminalistics: An Introduction to Forensic Science
 Biology 6th Ed.
 DNA fingerprinting:
www.bergen.org/EST/YEAR5/DNA_finger.htm
 Practical Applications of DNA Fingerprinting:
protist.biology.washington.edu/fingerprint/apps.html
 PCR: www.medicinenet.com
 Polymerase Chain Reaction:
www.faseb.org/opar/bloodsupply/pcr.html
 PCR: www.answers.com
 Wrongly convicted man...:
www.injusticebusters.com/05/bloodsworth_kirk.shtml
 Innocence Project:
www.innocenceproject.org/case/display_profile.php
 Polymerase Chain Reaction:
www.accessexcellance.org/RC/CT/polymearase_chain_reactio
n.html

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