PROJECT

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TO DETERMINE THE

QUALITY OF MILK IN JUJA


SUBCOUNTY,KIAMBU.

DENNIS MBITHI AGN221-0030/2020


CATHERINE WANYINGI AGN2210032/2020
ALVIN MWANIKI AGN221-0011/2020
RESEARCH METHODOLOGY
ESTIMATION OF THE TOTAL BACTERIAL
COUNT(TBC) IN THE COLLECTED MILK SAMPLES
- OBJECTIVE 1

 Spread plate technique is used to determine


the bacterial colony forming unit (Nisha,2022).

 CFM/ml= (number of colonies x dilution factor)


/ volume of culture plated)
MILK ADULTERANT TESTS ON STARCH
AND SPECIFIC GRAVITY - OBJECTIVE 2
 Laboratory test for starch in milk is carried out using
iodine test.
 Though the adulteration of milk with water can be
checked by lactometer reading, other adulterations
too affect the lactometer reading. Hence freezing point
depression, recognized by AOAC, is usually adopted.
 Percentage of water added = Normal freezing point –
Observed freezing point X 100
Normal
freezing point

 Normal freezing point of milk is taken as –0.55°C. A


tolerance level of 3% is given which is equivalent to
specifying a minimum freezing point depression for
authentic milk of –0.55°C.
TEST FOR THE PRESENCE OF
ANTIBIOTICS IN MILK
 The test will be carried using antibiotic milk
test kit (Tirlan ,2023).

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