Assessment Mass Production and Field Release

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Mass Production

Mass Production
Efficient Mass rearing
The basic Requirements for
successful insect colonization and
rearing were listed by Needham, et
al. (1937).
• Food
• Protection from enemies
• A suitable physical environment
• Fit conditions for reproduction
The components necessary in an
efficient mass-rearing operation
include:
1. The physical Facility
2. Diet(s)
3. Rearing containers
4. Rearing procedures
5. Qualified trained personnel
Rearing facilities
• Simple
• Practical
• functional
Diet
• Walker et al. list criteria for diet
suitability
1. High survival
2. Vigorous adults with high
reproductive capacity
3. Normal rate of development
4. Low-cost ingredients
5. Easy preparation for readily
available ingredients of uniform
Rearing Containers
Containers suitable for rearing
• Individual glass
• Plastic vials
• Cell webs
• Hexcel units
Rearing procedures and colony
• Adult Stageshandling
Ensure higher incidence of mating
by using large cages containing
host plants, with controlled
temperature and humidity, and 10%
honey solution for adult nutrition.
Ex. For H. sea. Used oviposition
cages.
Rearing procedures and colony
• Egg Stages handling
• Larvae – Depending on the
temperature the larvae mature
and begin pupating. The
development can be easily
observe for Plexiglas box.
• Pupal Stage Pupae are placed one
layer deep in boxes or dishes of various
Assessment of Parasitism

• Quantitative review showed that


biocontrol agents significantly
reduced pest abundance by 130%
compared to control groups,
increased parasitism by 139% and
overall pest mortality by 159%
compared to targets not exposed
to biocontrol agents.
Assessment of Parasitism

• Quantitative review showed that


most biocontrol studies were
focused on lepidopteran pests,
and that parasitoids were the
most common biocontrol agents
used..
Weed/Insect biocontrol agents
• Agent related types of
measurements can be made to quantify
damage caused by agents at different
densities on individual plants.
• Laboratory and glasshouse studies
simulate possible agent damage on
the target
weed can be performed ahead, to identify
the
• Field studies
Several approaches have been used
to assess the effectiveness of prospective
agents in the field.
• Demographic modelling
Models of plant population dynamics
can assist with the selection of effective
agents by identifying the most vulnerable
stages of the pest lifecycle.
• Benefit-cost analyses
To measure the expected or actual
return on investment from biological control
program which is expressed as benefit-cost
ratio or as net present value.
• Long-term evaluation
To check if the agents are performing
as expected and to understand the factors
that influence their effectiveness.
Augmentat
ion
and
Conservati
on
Augmentation
The release of natural enemies (predators,
parasites and pathogens) to control pests is a
type of biological control called augmentation.

2 Types:
• Inoculative
• Inundative
Conservation
Reduce the factors which interfere with natural
enemies or provide the resources that natural
enemies need in their environment

Ex. A study revealing the biological control of California


red scale, Aonidiella aurantii natural (Maskell), suggests
that the control may be achieved through periodic
washing of citrus tree foliage that increases the
parasitoids efficiency
Ex. Some host plant effects such as chemical defenses
which are harmful to natural enemies but the pest on
the host plant is best adapted to it, also reduces the
effectiveness of biological control. There are some pests
that are able to sequester toxic components of their
host, and use them as defense against their own
enemies. In such cases also the effectiveness of
biological control is reduced.
Importation
Importation of natural enemies, sometimes
referred to as classical biological control, is used
when a pest of exotic origin is the target of the
biocontrol program. Pests are constantly being
imported into countries where they are not native,
either accidentally, or in some cases,
intentionally. Many of these introductions do not
result in establishment or if they do, the organism
may not become pests.
IPM
1. Prevention: Organisms are kept from becoming problems
by planning and managing ecosystems.
2. Identification: Pests and beneficial organisms are
identified.
3. Monitoring: Pest and beneficial organism’s populations are
watched, as well as pest damage, and the
environment.
4. Injury and Action Decision: Injury and action thresholds
are used to know when to treat pests.
5. Treatments: Treatments (or a combination) are used,
including cultural, biological, physical,
mechanical, behavioural, or chemical
methods. The goal is to control pests with little impact
Advantages of IPM include:

ƒ Long-term answers to pest problems


ƒ Protecting environmental and human health by
reducing pesticide use
ƒ Reducing harm to beneficial organisms
ƒ Preventing creation of pesticide resistant
pests
ƒ Providing a way to manage pests when
pesticides cannot be used
Monitori
ng
Treatments
All available information is used in an IPM
program to select the best treatments. Two or more
treatments are often used together.
Most treatments fall into one of the following
groups:
• Cultural
• Mechanical/Physical
• Biological
• Behavioural
• Chemical

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