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Practical 1

Biosafety and bioethics

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17 views31 pages

Practical 1

Biosafety and bioethics

Uploaded by

iramashfaq741
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Basic laboratory safety measures

Introduction
• Laboratory safety rules are a major aspect of every microbiology
lab.
• Each student in microbiology laboratory must follow specific
safety rules and procedures.
Laboratory specific safety rules may be required for specific
processes, equipment, and materials, which should be
addressed by laboratory specific SOPs.
• Arrive on time for lab

If a student arrives late for lab and misses a substantial portion of the
introductory discussion and safety precautions, the student may be
a threat to themselves and others in the lab. The instructor may
deny the student the privilege of completing the assigned
experiment.
• Lab Coat
• The primary purpose of a lab coat is to protect against splashes
and spills. A lab coat should be nonflammable and should be
easily removed.
• We should not wear lab coats, gloves, or other personal
protective clothing outside of lab areas. This clothing may
become contaminated and could spread the contamination.
• Footwear

Leather shoes which completely cover the toes, heel and top of foot
provide the best general protection. The shoes must be made of
water proof materials. The shoe must have a nonslip sole firmly
attached to the foot.
• Gloves

When handling chemical, physical, or biological hazards that can


enter the body through the skin, it is important to wear the proper
protective gloves.
• Eyewear
• Safety goggles provide the best protection against chemical
splashes, vapors, and dusts. The event of an accident, chemicals
can get behind contact lenses and cause serious damage before the
lenses can be removed. If using contact lenses instead of glasses,
then we should wear eye-cup safety goggles in the lab.
• We should wear ultraviolet absorbing protective safety glasses
while working with ultraviolet light.
• Face Shields
• A face shield should be worn whenever there the entire face needs
protection (e.g., high pressure work, welding, soldering,
machining, fire, explosion, etc.).
• Respirators
• Respirators filter contaminants, either small airborne particles or
chemicals including gases.
• Respirators must be regularly cleaned, sanitized and maintained.
• Never eat or drink in the lab
• Food may pick up toxic chemicals.
• Never taste any chemical
• Some chemicals are very corrosive and poisonous in very small
quantities.
• Before you start an experiment, make sure you are fully aware of
the hazards of the materials you'll be using.
• Never perform an unauthorized experiment and never work in the
lab without an instructor in charge. An accident may happen when
mixing simple chemicals.
• Label all containers to identify their contents.
• Never put anything back into a reagent bottle Once a reagent has
passed the mouth of its container, it has passed the point of no
return. Always take as little of a chemical as possible. Use only
clean, dry spatulas for removing chemicals from bottles. Properly
dispose of excess chemicals.
• Leave chemicals in their proper place. Do not carry original

containers of chemicals to your benchtop.

• Clean up broken glassware immediately. Place it in the labeled

crock at the front of the lab. Obtain replacement glassware

from the instructor.


• Avoid touching hot objects
• Burns are a common accident in the chemistry lab. Be careful
when using hot plates and objects which have been heated on
them. Use beaker tongs to remove hot containers from the hot
plate.
• Clean up broken glassware immediately. Place it in the labeled
crock at the front of the lab. Obtain replacement glassware from
the instructor.
• Properly dispose of waste chemicals
• Certain liquids can be poured into the sink and flushed with water
while others are poured into designated waste containers. Most
solid wastes are placed in designated crocks. Your instructor will
provide disposal instructions each lab.
• Learn to locate and operate (if applicable), the safety shower, fire
extinguisher, eye-wash fountain, fire blanket, and fire exit.
• Dress properly for lab
• Wear clothing that covers as much skin as possible. Sandals are
not permitted. If possible, wear older clothes on lab day. All loose
clothing and long hair must be confined.
• Read the entire experiment before coming to lab. The instructor
will briefly discuss the experiment at the beginning of each lab.
• Keep the benchtop uncluttered

• Only those personal items pertinent to the lab work (lab manual,
etc.) are to be on the benchtop at a students work station. Book
bags, coats, etc. are not to be placed on the benchtop or on the
floor close to the lab benches. Place all such items in the
designated areas near the entrance to the lab.
• Keep drawers closed. Drawers and cabinets are to be kept closed
except when items are being taken from or returned of these
drawers.
• Take only planned breaks
• If the need arises to take a short break, you may do so at any time
during the experiment with these points in mind; try to plan the
break during a less critical time in the experiment (e.g. while
something is cooling); make sure that your hot plate is turned off;
inform a neighbor and the instructor.
• Do not pipette by mouth
• If you notice any unsafe conditions in the lab, let your supervisor
know as soon as possible.
• Always tie back hair that is chin-length or longer.
• Make sure that loose clothing or dangling jewelry is secured, or
avoid wearing it in the first place.
• Never wear shorts or skirts in the lab
• When working with Bunsen burners, lighted splints, matches, etc.,
acrylic nails are not allowed.
• After performing an experiment, you should always wash your
hands with soap and water.
• When using lab equipment and chemicals, be sure to keep your
hands away from your body, mouth, eyes, and face.
• Do not allow any solvent to come into contact with your skin.
• Chemicals should never be mixed, measured, or heated in front of
your face.
• Clean up at the end of lab. At the end of all lab sessions return
clean glassware to your drawer, clean your benchtop and finally
wash your hands thoroughly. Be sure all electrical devices and
water are turned off.
• If you are the last person to leave the lab, make sure to lock all the
doors and turn off all ignition sources.

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