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Extinction Coefficient

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P Saranya
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30 views8 pages

Extinction Coefficient

Uploaded by

P Saranya
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Molar extinction coefficient

Molar extinction coefficient


• The term molar extinction coefficient (ε) is a measure of how strongly
a chemical species or substance absorbs light at a
particular wavelength.
• It is an intrinsic property of chemical species that is dependent upon
their chemical composition and structure.
• The SI units of ε are m2/mol, but in practice they are usually taken as
M-1cm-1.
• The molar extinction coefficient is frequently used in spectroscopy to
measure the concentration of a chemical in solution.
• You can use the Beer-Lambert Law to calculate a chemical species’ ε:
• A = εLc
• Where:
• A is the amount of light absorbed by the sample for a particular wavelength
• ε is the molar extinction coefficient
• L is the distance that the light travels through the solution
• c is the concentration of the absorbing species per unit volume
• Rearrange the Beer-Lambert equation in order to solve for the molar
extinction coefficient:
• ε = A/Lc
Enzyme Activity

• The number of molecules of substrate transformed to products by


enzymes per unit of time is defined by enzyme activity.
• As a result, enzyme activity quantifies the number of lively enzyme
molecules in the process.
• This assessment, however, is affected by a variety of parameters,
including
• temperature, acid levels, substrate concentrations, enzyme intensity,
the inclusion of inhibitors as well as stimulators
• The frequency of overall enzyme reaction would be high whenever
the parameters regulating enzyme activity are appropriate.
• As a result, it is critical to maintaining ideal circumstances for enzyme
function to remain at its peak.
• Increased quantities of substrates plus enzyme may improve enzyme
activity under ordinary settings. In addition, the inclusion of inhibitors
may decrease enzyme activity.
• In comparison, the inclusion of activators, such as divalent ions, can
trigger the process while enhancing the enzyme’s function.
• Katal is the SI measure of such enzyme activity.
• 1 katal(kat) =1 mol/s
• However, because of practical limits, the most widely used
measurement has been called Enzyme Unit (U), that equals1 μmol
min-1.
specific activity

• specific activity of any enzyme tells its purity within any protein
composition.
• It quantifies the activities of a particular enzyme per milligram of
whole protein
• Specific activity=enzyme activity/total protein
• particularly significant in the separation of enzymes only from
specific proteins in an attempt to measure enzyme purity.
• The SI measure of such specific activity has been katal kg-1
• Common unit - μmol mg-1 min-1
• The proportion of substrate transformed by this enzyme into moles
per unit time has been referred to as enzyme activity.
• This determines the number of activated enzymes within a solution at
a particular moment.
• Specific activity, from the other side, would be the process of this
enzyme per milligram of the whole enzyme. It determines the
pureness of an enzyme within a combination

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