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Sulfate Analysis Spectrophotometry Method - English Report Text

The document is a lab report analyzing sulfate levels in tap water using spectrophotometry. [1] High levels of sulfate can harm human health, so the report analyzes a water sample to determine its sulfate concentration. [2] Using a spectrophotometer and the Lambert-Beer law, a standard curve is generated to quantify sulfate levels. [3] The results found the water sample's sulfate concentration to be 20.4758 mg/L, below the maximum level of 400 mg/L set by regulations.

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40 views11 pages

Sulfate Analysis Spectrophotometry Method - English Report Text

The document is a lab report analyzing sulfate levels in tap water using spectrophotometry. [1] High levels of sulfate can harm human health, so the report analyzes a water sample to determine its sulfate concentration. [2] Using a spectrophotometer and the Lambert-Beer law, a standard curve is generated to quantify sulfate levels. [3] The results found the water sample's sulfate concentration to be 20.4758 mg/L, below the maximum level of 400 mg/L set by regulations.

Uploaded by

nadazalfa.fitria
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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SULFATE

ANALYSIS IN TAP
Lab Report
WATER
Prepared to fulfill an English assignment from Mrs. Entin Sutinah, M.Pd

Nada Zalfa Fitria - XII APL 4


January 21, 2023
INTRODUCTION
Water is one of the most important thing for every living creatures on
earth. Water contains a lot of minerals and any other substances such as
sulfate.

Sulfate can be found on our environment and usually on its liquid form.
Chemical industrial and mine drainage wastes may discharge large
amounts of sulfate into watercourses, increasing its natural level to
several thousands of milligrams per liter.

High level of sulfate can cause damage to human’s health. In order to


know the quality of water, a check of the water is needed. One of the
most common used method to analyze sulfate level is spectrophotometry
method. According to Government Regulations No. 82 Year 2008, the
maximum amount of sulfate in water for hygiene needs is 400 mg/L.
LITERATURE REVIEW
Water is a chemical compound formed by a bound of two element of hydrogen and one
element of oxygen. Water is a useful important compound. Not all of water is good to be
used because water may contain a lot of substances without any change of physique.

Sulfate is a polyatomic ion. Sulfate is widely distributed in various natural environments


and plays an important role in biogeochemical cycles. Dissolved sulfate in water
environment is primarily derived from natural and anthropogenic sources. High sulfate
concentration can cause diseases and also places some constraints on industrial water
and irrigation water.

Spectrophotometry is a method to measure the concentration of a


substance by how it absorbs light by measuring the intensity of light
that passes through sample solution using an instrument called
spectrophotometer. Spectrophotometry work by the Lambert-Beer
Law.
MATERIALS AND APPARATUS
Visible SO42- Standard
Cuvette Vortex Mixer BaCl2
Spectrophotometer Solution

Demineralized Water Buffer Solution Stopwatch Spatula Test Tube Rack

Volumetric Flask Volumetric Pipette Measuring Pipette Test Tube


SPECTROPHOTOMETRY
1 METHOD
2 3
Pipette 25 ml of standard solution,
Make the standard curve of sulfate Add 1,25 ml of buffer solution
blank, and filtered sample into test
at 420 nm
tube

4 5 6
Wait for 5 minutes then check
Add one spatula of BaCl2, mix it the sample with Take a note of the absorbance and
with vortex mixer for one minute spectrophotometer at 420 nm concentration
immediately
RESULTS

STANDARD CURVE

Conc 1 Conc 2
Samples Df Abs 1 Abs 2
(mg/L) (mg/L)
CHECK
Standard - 0,4486 0,4486 53,6858 53,6858
RESULTS
Blank - 0,0035 0,0035 1,1823 1,1823

142/1 1 0,1771 0,1771 21,6596 21,6590


RESULTS
FINAL RESULTS
Mg SO42- = ( ((C1 - Cblk) x Df) + ((C2 - Cblk) x Df) )
2

Concentration
Samples
(mg SO42- /L)

Standard -

Blank -

142/1 (sample) 20,4758


DISCUSSION
Reaction: SO42- + BaCl2 BaSO4

White, crystalline
Filtered samples
solid compound

To get the better accuracy, check is


done in duplicate with maximum Samples Conc 1 (mg/L) Conc 2 (mg/L) RPD (%)
RPD at 5%
Standard 53,6858 53,6858 0,00

Blank 1,1823 1,1823 0,00

142/1 (sample) 21,6596 21,6590 0,00


CONCLUSION

Sulfate concentration in the tap water sample is


20,4758 mg/L.

The water sample meets the requirement of water for


hygiene needs in Government Regulations No. 82
Year 2008.
BIBLIOGRAPHY
Morais and António. 2001. Determination of Sulfate in Natural and Residual Water by Turbidimetric Flow-
Injection Analysis. Journal of AOAC INTERNATIONAL, 84(1): 59.

Wang, Huiwei and Qianqian Zhang. 2019. Research Advances in Identifying Sulfate Contamination
Sources of Water Environment by Using Stable Isotopes. Int J Environ Res Public Health, 16(11):
1914.

Ball, David W.. 2006. Field Guide to Spectroscopy. Bellingham: SPIE Press.
THANK
YOU!

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