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Biosafety

Sterilization methods include heat sterilization using autoclaving, chemical sterilization with agents like ethylene oxide, radiation sterilization using ionizing radiation, filtration through small pore filters to trap microorganisms, and plasma sterilization using low-temperature plasma to create lethal conditions. Each method has advantages for different materials, with heat and chemical sterilization commonly used for equipment and supplies, filtration for liquids and air, and radiation and plasma suitable for heat-sensitive items. Proper sterilization is important in medical and laboratory settings to eliminate microorganisms.

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8 views3 pages

Biosafety

Sterilization methods include heat sterilization using autoclaving, chemical sterilization with agents like ethylene oxide, radiation sterilization using ionizing radiation, filtration through small pore filters to trap microorganisms, and plasma sterilization using low-temperature plasma to create lethal conditions. Each method has advantages for different materials, with heat and chemical sterilization commonly used for equipment and supplies, filtration for liquids and air, and radiation and plasma suitable for heat-sensitive items. Proper sterilization is important in medical and laboratory settings to eliminate microorganisms.

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freakwrox
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BSL-1 (Basic Biosafety Level 1): This level BSL-2: Laboratories at this level handle

involves working with well-characterized agents moderate-risk agents that could cause diseases if
that pose minimal risk to humans and the exposed. Additional safety measures are taken,
including enhanced training, controlled access,
environment. Basic precautions are taken, such as
and appropriate waste management.
wearing lab coats and gloves.

BSL-3: These labs work with dangerous or exotic BSL-4: The highest level, BSL-4 labs, handle
agents that can cause serious diseases through extremely hazardous and potentially lethal agents.
inhalation. Strict containment measures are These facilities are equipped with stringent
employed, such as specialized equipment and containment systems to prevent any escape of
ventilation systems to prevent the release of agents, and personnel wear full-body protective
airborne agents. suits with independent air supplies.
In laminar air flow systems, air is typically filtered to remove particles and then directed in a consistent
direction over a work area or a specific environment. This helps prevent cross-contamination and
maintains a controlled space for tasks such as scientific experiments, medical procedures, and
manufacturing processes.

• Air Filtration
• Airflow Direction
• Unidirectional Flow
• Protective Barrier
• Particle Control

A Biological Safety Cabinet (BSC) is a protective enclosure used in laboratories to handle


hazardous materials, such as infectious agents or chemicals.

• Class I BSC: These cabinets provide personnel protection by directing air away from the user's
face, capturing and filtering airborne particles and gases.

• Class II BSC: The most common type, Class II BSCs provide both personnel and
environmental protection. They have a filtered exhaust to prevent contamination release and a
downflow of HEPA-filtered air that protects the samples being worked on.

• Class III BSC: Also known as glove boxes, these cabinets provide the highest level of
protection. They are completely enclosed and are used to handle extremely hazardous materials,
such as highly infectious agents.
1. Heat Sterilization: This method involves using high
temperatures to kill microorganisms. Autoclaving is an example.

2. Chemical Sterilization: Chemical agents such as ethylene


oxide or hydrogen peroxide vapor can be used to sterilize items
that are sensitive to heat.

3. Radiation Sterilization: Ionizing radiation can penetrate


materials to kill microorganisms. This method is often used for
medical supplies and certain foods.

4. Filtration: This method involves passing liquids or gases


through a filter with pores small enough to trap microorganisms.
It's commonly used for liquids and air.

5. Plasma Sterilization: Low-temperature plasma can be used


to sterilize heat-sensitive items by creating an environment that
is lethal to microorganisms.

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