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    Applications of Molecular Probe

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    Zille Huma
    This document discusses molecular probes and their applications. Probes can be obtained from purified mRNA, cDNA, cloned DNA segments, asymmetric PCR, or synthetic oligonucleotides. Probes have various applications, including studying evolutionary relationships through restriction fragment length polymorphisms, identifying genetic disorders through DNA polymorphism analysis, DNA fingerprinting using variable number tandem repeats, isolating genes using homologous or heterologous probes, and identifying chromosomes through in situ hybridization.

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    Applications of Molecular Probe

    Uploaded by

    Zille Huma
    18 views16 pages
    This document discusses molecular probes and their applications. Probes can be obtained from purified mRNA, cDNA, cloned DNA segments, asymmetric PCR, or synthetic oligonucleotides. Probes have various applications, including studying evolutionary relationships through restriction fragment length polymorphisms, identifying genetic disorders through DNA polymorphism analysis, DNA fingerprinting using variable number tandem repeats, isolating genes using homologous or heterologous probes, and identifying chromosomes through in situ hybridization.

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    This document discusses molecular probes and their applications. Probes can be obtained from purified mRNA, cDNA, cloned DNA segments, asymmetric PCR, or synthetic oligonucleotides. Probes have various applications, including studying evolutionary relationships through restriction fragment length polymorphisms, identifying genetic disorders through DNA polymorphism analysis, DNA fingerprinting using variable number tandem repeats, isolating genes using homologous or heterologous probes, and identifying chromosomes through in situ hybridization.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    18 views16 pages

    Applications of Molecular Probe

    Uploaded by

    Zille Huma
    This document discusses molecular probes and their applications. Probes can be obtained from purified mRNA, cDNA, cloned DNA segments, asymmetric PCR, or synthetic oligonucleotides. Probes have various applications, including studying evolutionary relationships through restriction fragment length polymorphisms, identifying genetic disorders through DNA polymorphism analysis, DNA fingerprinting using variable number tandem repeats, isolating genes using homologous or heterologous probes, and identifying chromosomes through in situ hybridization.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 16

    Molecular probe

    Applications of molecular probe


    Molecular probe

    • What are probes?


    • Labelling of probs
    • How probes
    obtained?
    • Applications of
    molecular probes
    Probes can be obtained by-
    • 1.Highly purified mRNA can be used as a probe mRNA
    are naturally single stranded.
    • 2. Single Stranded cDNA probes can be prepared by
    limiting the copy of mRNA by reverse transcriptase of
    only one strand.
    • 3. Single Stranded DNA probes can be prepared by
    cloning the concerned DNA segment in a vector like M-
    13 vector of E.coli .
    Probes can be obtained by-
    • 4. By assymetric-PCR.PCR can be used to generate
    single stranded copies of a DNA sequence.
    • 5. Synthetic oligonucleotides Can be prepared for use
    as a probe.
    Applications of molecular probes
    RFLPs for Evolutionary Studies
    • The restriction fragment length polymorphisms
    (RFLPs) can be studied in a set of related species
    using a random or a specific DNA probe.
    • The similarities and differences can be used to infer
    phylogenetic relationships.
    RFLP Markers to Map the Genes in Diseased
    Persons and Identification of Disease

    DNA polymorphisms (many forms) are differences


    in DNA sequence that result from point mutations,
    random deletions or insertions.
    It is now possible to detect polymorphisms in
    unexpressed regions of DNA by this analysis.
    Genetic disorders like sickle cell anemia,
    Thalassemia’s can be identified by probes.
    USE OF MOLECULAR PROBES IN DNA
    FINGER PRINTING
    • DNA fingerprinting uses a specific type of DNA
    sequence known as Variable Number Tandem
    Repeats (VNTRs) can contain anywhere from
    twenty to one hundred base pairs.

    • Every human being has some VNTRs. To


    determine if a person has a particular VNTR, a
    southern Blot is performed and then the
    southern blot is probed through a hybridization
    reaction.
    Isolation of Genes Using DNA or RNA
    Probes
    .

    Homologous Probes

    Heterologous probes
    In situ Hybridization
    The method is based on the formation of
    double stranded hybrid molecules which form
    between a DNA or RNA target sequence and
    the complementary single standard labeled
    probe.
    In a number of cases rye chromosomes in
    wheat background have been identified using
    this technique.
    Thanks

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