Pure Line Selection
Pure Line Selection
Pure Line:
It is the progeny of single self- fertilized
homozygous plant.
Pure Line Selection:
In pure line selection, large numbers of plants
are selected from a self-pollinated crop and is
harvested individually, individual plant
progenies from them are evaluated separately
and the best one is released as pure line
variety. Therefore it is also known as
individual plant selection.
Characteristics of Pure Line
1. All plant within a pure line has same genotype
as the plants from which the pure lines are
derived.
2. The phenotypic differences (variation) within a
pure line is environmental and therefore non
heritable.
3. The pure line becomes genetically variable with
time, due to mechanical mixture, mutation, etc.
Uses of Pure Line
1. Superior line is used as variety.
2. It is used as parent in development
of new variety by hybridization.
3. Pure lines are used for studying
mutations and other biological
investigations such as medicine,
immunology, physiology, and
biochemistry.
Applications of Pure Line Selection
It is used for improvement of local varieties
It is used for improvement of old pure line
varieties
It provides an opportunity for selection of
new characteristics, such as disease resistance,
grain type , plant type, etc
It provides an opportunity for selection in the
segregating generation from crosses.
Procedure of Pure Line Selection
The pure line selection has three steps.
1. Selection of individual plants from a
local variety or from mixed population.
2. Visual evaluation of individual plant
progenies.
3. Yield Trials.
First Year
Select large number of plants (200-3000) from
local variety or some other mixed population
and their seeds are harvested separately. In
case of individual plants can’t be identified
individual heads may be selected on the basis
of easily observable characters, such as
flowering, maturity duration, disease,
resistance, presence of awns , plant height etc.
It is advisable to select plants for easily
observable characteristics
Second Year
Selected individual plants progenies are grown
with proper spacing weak along with standard
variety row. Progenies are evaluated visually
and poor weak and defective segregating
progenies are rejected on the basis of visual
characteristics. The member of progenies
selected should be less to facilitate replicated
yield trials if necessary this process may be
repeated for one or more years.
Third Year
Grow the selected progenies in a replicated trails
for critical evaluation. The best variety is used as
a check for comparison and planted after every
20-25 progenies. If sufficient seeds are available,
preliminary yield trial may be conducted.
Selection is made for easily observable,
preliminary yield trial may be conducted.
Selection is made for easily observable characters
including disease resistance and numbers of
progenies are reduced.
Fourth to Seventh Year
Replicated main yield trails are conduced using
best variety as a check quality test is also
conducted and used as a basis of selection.
Each progeny is an experimental stain as it is
pure line. The promising strains are evaluated
at several locations along with other strains in
coordinated yield trials. The most promising
strains are identified.
Eight Year
The best progeny is released as a new
variety and its seed is multiplied for
distribution to farmers.
Merits of Pure Line Selection Method