Blood Collection
Blood Collection
Blood Collection
Org
Blood Collection
2
Definition
Venipuncture is
the most
common
method of
obtaining a
diagnostic blood
specimen.
3
Introduction
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Safety Measures
• Gloves are to be worn during all phlebotomies, and
changed between patient collections.
• Palpation of phlebotomy site may be performed
without gloves providing the skin is not broken.
• A lab coat or gown must be worn during blood
collection procedures.
• Needles and hubs are single use and are disposed of
in an appropriate ‘sharps’ container as one unit.
• Needles are never recapped, removed, broken, or
bent after phlebotomy procedure.
●●●
8
Safety Measures
• Gloves are to be discarded in the appropriate
container immediately after the phlebotomy
procedure.
• All other items used for the procedure must be
disposed of according to proper biohazardous
waste disposal policy.
• Contaminated surfaces must be cleaned with
freshly prepared 10% bleach solution. All surfaces
are cleaned daily with bleach.
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Procedure of Blood Collection
• Identify the patient, two forms of active
identification are required.
Ask the patient to state their name and date of
birth.
This information must match the requisition.
• Reassure the patient that the minimum
amount of blood required for testing will be
drawn.
• Verify that any diet or time restrictions have
been met.
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10
Procedure of Blood Collection
Order of Draw
• The following order of draw is the approved
order as established by CLSI.
• This order of draw should be followed
whenever multiple tubes are drawn during a
single venipuncture.
• This is to prevent cross contamination by the
tube additives that could lead to erroneous
results.
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11
Blood Culture
• Light Blue Top (plasma): 3.2% sodium citrate.
These tubes are used for coagulation tests and
need to be completely filled to ensure the
proper ratio of blood to anticoagulant.
• Red Top (serum): Plain and gel. Used for
chemistry and reference tests.
• Green Top (plasma): With and without gel,
contains lithium heparin. These tubes are used
primarily for chemistry tests.
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12
Blood Culture
• Lavender or Pink Top (plasma): Contains EDTA.
Used primarily for hematology and blood bank
testing.
• Gray Top (plasma): Contains sodium
fluoride/potassium oxalate. Used by chemistry
for glucose testing.
• Yellow Top (plasma and cells): Contains ACD
solution A or B. Used for Genetics testing.
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14
Conclusion
15
References
• Google.com
• Wikipedia.org
• Studymafia.org
• Slidespanda.com
Thanks
To
StudyMafia.org