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Low and High Voltage Electrophoresis: Movement of Charged Particles Under The Influence of The Electric Current

This document discusses low and high voltage electrophoresis. [1] Low voltage electrophoresis uses a basic apparatus with a power pack and electrophoresis unit to separate ions using either a horizontal or vertical strip. It is used to examine biological samples for proteins and amino acids. [2] High voltage electrophoresis also uses a basic apparatus but requires cooling. It can use either an enclosed or immersed strip method. It is used to separate low molecular weight substances like amino acids.

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Poonam Katyal
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1K views18 pages

Low and High Voltage Electrophoresis: Movement of Charged Particles Under The Influence of The Electric Current

This document discusses low and high voltage electrophoresis. [1] Low voltage electrophoresis uses a basic apparatus with a power pack and electrophoresis unit to separate ions using either a horizontal or vertical strip. It is used to examine biological samples for proteins and amino acids. [2] High voltage electrophoresis also uses a basic apparatus but requires cooling. It can use either an enclosed or immersed strip method. It is used to separate low molecular weight substances like amino acids.

Uploaded by

Poonam Katyal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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LOW AND HIGH VOLTAGE

ELECTROPHORESIS

Movement of charged particles under the influence of the electric


current
CONTENT
 INTRODUCTION

 LOW VOLTAGE ELECTROPHORESIS


• INSTRUMENTAION
• APPLICATION

 HIGH VOLTAGE ELECTROPHORESIS


• INSTRUMENTATION
• APPLICATION
INTRODUCTION
• Separations technique that is based on the
mobility of ions in an electric field.
• Types of electrophoresis:

GEL LOW AND HIGH


ELECTROPHORESIS VOLTAGE
ELECTROPHORESIS

CAPILLARY CONTINOUS
ELECTROPHORESIS ELECTROPHORESIS
LOW VOLTAGE
ELECTROPHORESIS
INSTRUMENTATION [GENERAL
APPARATUS]
• It consists basically of two items a power pack
and an electrophoresis unit.
POWER PACK
ELECTROPHORESIS UNIT
 Electrodes
 Buffer reservoir
 Support for electrolysis medium
 A transparent insulating cover.
LOW VOLTAGE
ELECTROPHORESIS
The apparatus commonly used for low voltage
paper electrophoresis is of two types:

  HORIZONTAL STRIP

VERTICAL STRIP
HORIZONTAL STRIP

Principle: To separate any ionic substances


VERTICAL STRIP
APPLICATION OF LOW VOLTAGE
ELECTROPHORESIS

• In examination of biological and clinical


specimens for amino acids and proteins.

• Use for separation of proteins and other high


molecular weight substance.
HIGH VOLTAGE
ELECTOPHORESIS
INSTRUMENTATION(GENERAL
APPARATUS)
• The equipment consists basically of two items a
power pack and an electrophoresis unit.
POWER PACK
ELECTROPHORESIS UNIT

 Electrodes
 Buffer reservoir
 Support for electrolysis medium
 A transparent insulating cover.
 Cooling Plate.
HIGH VOLTAGE ELECTROPHORESIS
• The apparatus commonly used for high voltage
paper electrophoresis is of two types:

ENCLOSED STRIP

IMMERSED STRIP
ENCLOSED STRIP
• In principle, paper electrophoresis is simpler
than paper chromatography. 

•  There are two features of an ion which are


most pertinent to its electrophoretic
behavior; the size of the ion and the size of
the charge it carries.  

• These features oppose each other; the bigger


the size the slower the ion will travel; the
bigger the charge the faster it will go.
IMMERSED STRIP METHOD
WORKING:
• In this method the sample is applied to a strip
of filter paper maintained with buffer solution
and the strip is immersed in a large volume of
buffer- immiscible, electrically insulating and
volatile cooling liquid.

• Apparatus can also be designed to accumulate


cooling liquids which have a higher density than
that of buffer solution.
APPLICATION OF HIGH VOLTAGE
ELECTROPHORESIS
• High voltage electrophoresis has been
successfully used in the separation of low
molecular weight substance such as amino acids,
peptide, Indoles, sugars, Purines, Pyrimidines,
amines and inorganic ions.

• The amino acid composition of two unknowns can


be determined.
THANK YOU

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