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BSL and Protocols in Experimenting

This document discusses biosafety levels and protocols for scientific experiments. It defines biosafety levels as classifications used to identify protective measures needed in laboratories to protect workers, the environment and the public. The four biosafety levels are described from BSL-1 posing low risk to BSL-4 posing very high risks. Example agents are provided for each level along with descriptions of the typical containment facilities and practices required.

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Mayleen Buenavista
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254 views40 pages

BSL and Protocols in Experimenting

This document discusses biosafety levels and protocols for scientific experiments. It defines biosafety levels as classifications used to identify protective measures needed in laboratories to protect workers, the environment and the public. The four biosafety levels are described from BSL-1 posing low risk to BSL-4 posing very high risks. Example agents are provided for each level along with descriptions of the typical containment facilities and practices required.

Uploaded by

Mayleen Buenavista
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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RESEARCH I

Biosafety Level
and Protocols in
Experimenting
MELC
The learner should be able to
perform scientific experiment; SSP
_RS&-IPS-III-m-n-15
OBJECTIVES
• Define protocols and biosafety level.
• Enumerate and explain the purposes of the rules for conducting
science investigatory projects.
• Explain the classifications of Biosafety Levels (BSL).
PURPOSES OF
RULES
• It protects the right and welfare of the student
researcher.
• It protects the rights and welfare of human
participants.
CONTINUATI
ON

• To protect the health and welfare of vertebrate


animal subjects.
• Protect and promote good stewardship of the
environment.
CONT.

• Ensure adherence to federal regulations.


• Ensure the use of safe laboratory practices.
• Determine eligibility for competition in the Intel ISEF.
PROTOCOL
• is a system of rules that explain the correct
conduct and procedures to be followed in
formal situations.
PROTOCOL
• it is a plan for a scientific experiment or
for medical treatment.
PROTOCOL
• is a document that describe the details of a
treaty or formal agreement between
countries.
BIOLOGICAL
SAFETY LEVELS
• are used to identify the protective
measures needed in a laboratory setting to
protect workers, the environment, and the
public.
CLASSIFICATION OF
BIOLOGICAL AGENTS RISK
GROUPS
BSL - 1 RISK
GROUP
• BSL - 1 risk group contains biological
agents that pose low risk to personnel and
the environment.
EXAMPLES

AGROBACTERIUM
TUMEFACIENS
EXAMPLES

MICROCOCCUS
LUTEUS
EXAMPLES

NEUROSPORA CRASSA
EXAMPLES
BACILLUS SUBTILIS
Bacillus subtilis, known as the hay
bacillus or grass bacillus is a Gram-
positive, catalase-positive bacterium,
found in soil and the gastrointestinal tract
of ruminants and humans.
BSL-1 LAB
BSL-1 labs are used to study infectious
agents or toxins not known to
consistently cause disease in healthy
adults.
BSL-1 LAB
BSL - 2 RISK
GROUP
• BSL - 2 risk group contains biological
agents that pose moderate risk to
personnel and the environment.
EXAMPLES
Mycobacterium are the causative
organisms for diseases such as
tuberculosis (TB), leprosy, Buruli ulcer,
and pulmonary nontuberculous
mycobacterial disease, to name the most
important ones.

MYCOBACTERIUM
EXAMPLES
• Streptococcus pneumoniae are lancet-
shaped, gram-positive, facultative
anaerobic bacteria with known 100
serotypes.
• can cause disease, but only a minority
of serotypes produce the majority of
pneumococcal infections.
STREPTOCOCCUS
PNEUMONIAE
EXAMPLES
• S. choleraesuis causes blood
poisoning in some hogs but in latent
in others, which act as carriers.
Apparently healthy pets-e.g. dogs,
cats, and turtles and other reptiles can
through close contact, transmit
salmonellosis to humans.
SALMONELLA CHOLERAESUIS
BSL - 3 RISK
GROUP
• BSL - 3 risk group contains biological that usually
cause serious disease (human, animal or plant) or
that can result in serious economic consequences.
Projects in the BSL-3 group are prohibited.
BSL-3 LAB
• BSL-3 laboratories are used to study
infectious agents or toxins that may be
transmitted through the air and cause
potentially lethat infection through inhalation
exposure.
• Researchers perform all experiments in
biosafety cabinets that use carefully
controlled air flow or sealed enclosures to
prevent infection.
BSL-3 LAB
• BSL-3 laboratories are designed to be easily
decontaminated.
• These laboratories must use controlled or
"directional", air flow to ensure that air flows
from non-laboratory areas (such as hallway)
into laboratory areas as an additional safety
measure.
BSL - 4 RISK
GROUP
• BSL - 4 risk group contains biological that usually
produce very serious disease (human, animal or
plant) that is often untreatable. Projects in the BSL-4
group are prohibited.
BSL-4 LAB
• BSL-4 laboratories are used to study
infectious agents or toxins that pose a high
risk aerosol-transmitted laboratory infections
and life-threatening disease for which no
vaccine or therapy is available.
• Access to BSL-4 laboratories is carefully
controlled and requires significant training.
2 TYPES OF BSL-
4 LAB

• Cabinet laboratory - all work with infectious agents or toxins is done in a Class III Biosafety Cabinet
with very carefully designed procedures to contain any potential contamination. In addition, the
laboratory space is designed to also prevent contamination of other places.
2 TYPES OF BSL-
4 LAB

• Suit laboratory - laboratory personnel are required to wear full-body


air-supplied suits, which are the most sophisticated type of personal
protective equipment.
2 TYPES OF BSL-
4 LAB
• all personnel shower
before exiting the
laboratory and go
athrough a series of
procedures designed to
fully decontaminate them
before leaving.
LEVELS OF BIOLOGICAL
CONTAINMENT
BSL - 1
CONTAINMENT
• BSL - 1 containemnt is normally found in
water-testing laboratories, in high schools, and
in colleges teaching introductory microbiology
classes.
BSL - 1
CONTAINMENT
• BSL - 1 containment is normally found in water-testing
laboratories in high schools, and in colleges teaching
introductory microbiology classes.
• Work is done on an open bench or in an appropriate
biosafety hood. Standard microbiological practices are used
when working in the laboratory.
BSL - 2
CONTAINMENT
• BSL - 2 containment is designed to maximize safety when
working with agents of moderate risk to humans and the
environemnt. Access to the laboratory is restricted.
• Decontamination can be achieved by treating with chemical
disinfectants or by steam autoclaving.
BSL - 3
CONTAINMENT
• BSL - 3 containment is required for infectious agents that
may cause serious or potentially lethal diseases as a result
of exposure by inhalation.
BSL - 4
CONTAINMENT
• BSL - 4 containment is required for dangerous/exotic
agents taht pose high risk of life-threatening disease.
Projects in the BSL-4 group are prohibited.
SUMMARY
SUMMARY

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