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    Tissue Culture Technology in Arecanut

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    Sasira Deivasigamani
    This document presents information on tissue culture technology for arecanut (betel nut). It discusses the need for tissue culture to mass multiply dwarf arecanut varieties and hybrids that are resistant to the devastating yellow leaf disease. It describes the standardized tissue culture protocol developed by CPCRI in Kasaragod, India using inflorescence explants. This protocol has been used to propagate over 60 arecanut palms that have similar growth and reproduction as seed-borne plants. Future areas of research discussed include strengthening germplasm collections, developing molecular markers, regeneration protocols, and abiotic stress tolerance screening.

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    Tissue Culture Technology in Arecanut

    Uploaded by

    Sasira Deivasigamani
    58 views14 pages
    This document presents information on tissue culture technology for arecanut (betel nut). It discusses the need for tissue culture to mass multiply dwarf arecanut varieties and hybrids that are resistant to the devastating yellow leaf disease. It describes the standardized tissue culture protocol developed by CPCRI in Kasaragod, India using inflorescence explants. This protocol has been used to propagate over 60 arecanut palms that have similar growth and reproduction as seed-borne plants. Future areas of research discussed include strengthening germplasm collections, developing molecular markers, regeneration protocols, and abiotic stress tolerance screening.

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    Tissue culture technology in arecanut

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    This document presents information on tissue culture technology for arecanut (betel nut). It discusses the need for tissue culture to mass multiply dwarf arecanut varieties and hybrids that are resistant to the devastating yellow leaf disease. It describes the standardized tissue culture protocol developed by CPCRI in Kasaragod, India using inflorescence explants. This protocol has been used to propagate over 60 arecanut palms that have similar growth and reproduction as seed-borne plants. Future areas of research discussed include strengthening germplasm collections, developing molecular markers, regeneration protocols, and abiotic stress tolerance screening.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    58 views14 pages

    Tissue Culture Technology in Arecanut

    Uploaded by

    Sasira Deivasigamani
    This document presents information on tissue culture technology for arecanut (betel nut). It discusses the need for tissue culture to mass multiply dwarf arecanut varieties and hybrids that are resistant to the devastating yellow leaf disease. It describes the standardized tissue culture protocol developed by CPCRI in Kasaragod, India using inflorescence explants. This protocol has been used to propagate over 60 arecanut palms that have similar growth and reproduction as seed-borne plants. Future areas of research discussed include strengthening germplasm collections, developing molecular markers, regeneration protocols, and abiotic stress tolerance screening.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 14

    TISSUE CULTURE TECHNOLOGY

    IN ARECANUT
    PRESENTED BY
    . SASIRA.D, 2018006039
    NEED FOR TISSUE CULTURE
    TECHNOLOGY IN ARECANUT
    • Mass multiplication of dwarf arecanut.
    ( variety – Hirehalli) and it’s hybrids

    • One of the major production constraints of


    arecanut – devastating yellow leaf disease
    ( YCD).
    YELLOW LEAF DISEASE. DWARF
    HYBRIDS
    Tissue culture seems to be the
    only vegetative propagation
    tool applicable to areca palm.
    TISSUE CULTURE PROTOCOL AT CPCRI:

    • Arecanut tissue culture protocol – standardized by


    CPCRI, Kasaragod.
    • Through Inflorescence explant.
    • For rapid multiplication of genotypes such as
    * Yellow Leaf Disease ( YCD) resistant palms.
    * Dwarf hybrids development.
    ESTABLISHMENT OF PROTOCOL

    • More than 60 arecanut palms propagated


    using this protocol, planted in different
    locations.
    • Their growth and reproduction are similar
    to seed – borne plants.
    FUTURE THRUST AREAS OF PALM:

    • Strengthening of germplasm collection and characterization of


    different traits for utilization in breeding programmes
    • Cryopreservation of germplasm and development of molecular
    markers for marker assisted selection (MAS) in
    breedingprogrammes.
    • Development of regeneration protocol.
    FUTURE THRUST AREAS OF PALM:

    • Development of theoretical ideotype forfocused crop


    improvement
    • Understanding the abiotic stress tolerance and screening
    germplasm for tolerance to different stresses.
    • Development of integrated eco friendly crop protection
    PROTOCOL FOR IN VITRO
    PROPAGATION IN
    ARECANUT
    MEDIA REQUIREMENTS FOR GROWTH:

    • M.S media, White media, Branton and Blake’s (BB) media were used.
    • GROWTH REGULATORS ; Auxin + Giberellins, 2,4-D were used.
    • Darkening effect of activated charcoal – induced
    rooting in shoot cultures.
    • Synergistic action of abscissic acid and auxin –
    enhanced the frequency of rooting.
    OBSERVATIONS AND RESULTS:

    Within 4 weeks,
    • Greenish soft callus formed.
    • 90% of shoots formed after 4 weeks.
    After 7-8 weeks
    • Darkness wounded regions of explants
    . Formed callus with yellow soft, glutinous structures
    OBSERVATIONS AND RESULTS:

    • The embryos developed into plantlets after 10weeks


    of culture on basal medium free of plantgrowth
    regulators.
    • After subculturing everymonth for three months the
    plantlets weretransferred to containers for
    acclimatization inthe green house.
    • The survival rate was 24 %.
    REFERENCE WEBSITES :

    1. agritech.tnau.ac.in
    2. https://icar.org.in
    3. www.researchgate.net
    THANK YOU
    FOR
    YOUR PATIENCE !

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