Instrument Processing
Instrument Processing
PROCESSING
Doc Dyann Pena
• A process intended to kill all microorganisms
• The highest level of microbial kill that can be achieved
Sterilization • The bacterial endospore is selected as the standard challenge
for sterilization because of its high resistance to killing by
heat and chemicals.
3 Types of
Sterilization
• Heat Sterilization
• Steam / dry heat / unsaturated chemical vapor
• Most common type in dental offices
• Operates at 121-190.6 deg Celsius
• Gas Sterilization
• Not commonly used due to high cost and long
exposure time required
• Remove bioburden
• Reduce the direct handling of
instruments
• Can clean loose instruments in
baskets or instruments in cassettes.
Precleaning
(Manual Scrubbing of Instruments)
• Dangerous; even though the procedure is an
effective method of removing debris, if
performed properly.
• Packaging involves
wrapping cleaned
instrument cassettes and
wrapping them or placing
them in sterilization
pouches, bags, trays, or
cassettes.
• The heat, not the pressure, inside a steam sterilizer is what actually kills
the microorganisms.
• A typical dental office steam sterilizer usually operates through four steps:
• heat-up cycle
• sterilizing cycle
• 121° C (250° F) for 30 minutes
• 121° C (250° F) for 15 minutes
• 134° C (273° F) for 10 minutes
• 134° C (273° F) for 3 minutes
• depressurization cycle
• drying cycle
• Do not stack packages, pouches, or cassettes flat in layers.
• Keep packs, pouches, or cassettes separated from each
other so that steam has access to all package surfaces.
• Drying packages inside the steam sterilizer is important to
maintain the sterility of the instruments.
Dry Heat Sterilization
• Heating air with transfer of heat energy from the air to the
instruments.