Topic 2: DNA and Proteins
Lesson 2.2 DNA and Protein Synthesis
This lesson covers:
From Crierie, Greig, Ruthven (2019), this lesson explores the following Science
Understanding components as they appear on p. 17:
A gene consists of a unique sequence of nucleotides that code for a functional protein or an RNA molecule.
• Distinguish between exons and introns as coding and non-coding segments of DNA found in genes
• Describe how both exons and introns are transcribed but only the information contained in exons will be
translated to form a polypeptide
Protein synthesis involves transcription of a gene into messenger RNA (mRNA) and translation of mRNA into an
amino acid sequence at the ribosomes. In eukaryotic cells, transcription occurs in the nucleus.
• Describe and illustrate the role of DNA, mRNA, transfer RNA (tRNA) and ribosomal RNA (rRNA) in transcription
and translation
• Describe the relationship between codons, anticodons and amino acids
Genes & Chromosomes
Chromosome
Segment of DNA that contains genes
Gene
Section of a chromosome that codes for a
protein
Exons and Introns
Not all DNA codes for proteins.
DNA
Exon Intron
Ribosomal RNA
Intron Non-coding DNA INTRON RNA Transfer RNA
Micro RNA
Exon Coding DNA
EXON mRNA PROTEIN
Protein synthesis Production of proteins through use of DNA
Amino
3 important molecules Acid
Messenger RNA (mRNA)
Produced when DNA is read in
transcription
Single-stranded
Thymine is replaced with uracil
Sets of bases grouped by three Anti-codon
(codon)
Transfer RNA (tRNA)
DNA Places amino acids in translation to
form proteins
Two complementary strands Consists of an anticodon
Contains genes as sequences of complementary in bases to an mRNA
nitrogen bases codon
Ribosomes
Site of DNA translation.
Made up of ribosomal RNA (rRNA).
Amino Acids
20 amino acids
Amino Acids
mRNA codon that codes for a specific
amino acid.
mRNA codons match with tRNA
complementary anticodons
tRNA with anticodon that is complementary
to mRNA codon will place its amino acid
into a polypeptide chain
This polypeptide chain becomes a protein
Protein Synthesis
Two steps:
Transcription Transfer of DNA information into mRNA
Translation mRNA read by ribosomes. Protein formed from aa chain.
Transcription
DNA unravelled by RNA polymerase
enzyme
Coding strand
Template strand
Free mRNA nucleotides align to complementary nucleotides on template strand of
DNA to form single-stranded ‘pre-mRNA’ molecule
Transcription – pre-mRNA to
mRNA
RNA Splicing used to remove introns from
mRNA molecule
Translation
mRNA passes into the cytoplasm and
threads through a ribosome
A start codon initiates polypeptide chain
construction
Each subsequent codon matches with
specific tRNA anticodon, which adds amino
acid to polypeptide chain connected
through bonds (chain elongation)
Stop codon terminates the chain
Further resources
Transcription & Translation
https://www.youtube.com/watch?v=bKIpDtJdK8Q
Types of RNA
https://www.news-medical.net/life-sciences/-Types-of-
RNA-mRNA-rRNA-and-tRNA.aspx