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    Adsorption Chromatography:: Presented By: Aarohi Thakur ADM. NO. H-2019-05-001

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    Akshit Kapila
    This document discusses adsorption chromatography, which separates mixtures based on how compounds adsorb to a stationary phase. It works by having a mobile phase carry compounds through an adsorbent stationary phase at different rates based on their bonding strengths. There are several types including column chromatography using a packed column, thin-layer chromatography on coated plates, and gas-solid chromatography using an inert gas as the mobile phase. Applications include separating organic compound mixtures and identifying carbohydrates and lipids.

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    Adsorption Chromatography:: Presented By: Aarohi Thakur ADM. NO. H-2019-05-001

    Uploaded by

    Akshit Kapila
    12 views15 pages
    This document discusses adsorption chromatography, which separates mixtures based on how compounds adsorb to a stationary phase. It works by having a mobile phase carry compounds through an adsorbent stationary phase at different rates based on their bonding strengths. There are several types including column chromatography using a packed column, thin-layer chromatography on coated plates, and gas-solid chromatography using an inert gas as the mobile phase. Applications include separating organic compound mixtures and identifying carbohydrates and lipids.

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    H-2019-05-001

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    This document discusses adsorption chromatography, which separates mixtures based on how compounds adsorb to a stationary phase. It works by having a mobile phase carry compounds through an adsorbent stationary phase at different rates based on their bonding strengths. There are several types including column chromatography using a packed column, thin-layer chromatography on coated plates, and gas-solid chromatography using an inert gas as the mobile phase. Applications include separating organic compound mixtures and identifying carbohydrates and lipids.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    12 views15 pages

    Adsorption Chromatography:: Presented By: Aarohi Thakur ADM. NO. H-2019-05-001

    Uploaded by

    Akshit Kapila
    This document discusses adsorption chromatography, which separates mixtures based on how compounds adsorb to a stationary phase. It works by having a mobile phase carry compounds through an adsorbent stationary phase at different rates based on their bonding strengths. There are several types including column chromatography using a packed column, thin-layer chromatography on coated plates, and gas-solid chromatography using an inert gas as the mobile phase. Applications include separating organic compound mixtures and identifying carbohydrates and lipids.

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    © All Rights Reserved
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    of 15

    Adsorption

    Chromatography:
    PRESENTED BY: AAROHI THAKUR
    ADM. NO. H-2019-05-001
    Chromatography:

     Chromatography is a physico-chemical method for


    separation of compound mixtures, based on the
    distribution of components between two phases, one of
    which is stationary (sorbent), and the other, mobile,
    flowing through a layer of the stationary phase.
    Principle:

     Chromatography is based on the principle where


    molecules in mixture applied onto the surface or into the
    solid, and fluid stationary phase (stable phase) is
    separating from each other while moving with the aid of a
    mobile phase.
    Adsorption Chromatography:

     Itis defined as a technique for the seperation of the


    component in a mixture by adsorption from a mobile
    phase into the stationary solid surface. 
    Principle:
     Adsorption chromatography is based on the principle that
    some solid substances, called adsorbents, have the power
    to hold molecules at their surface. This bonding force is
    due to weak, non- ionic attractive forces which only occur
    at specefic adsorption beds. 
     
    Adsorption Chromatography Experiment
    (TLC):
     Take a clean and dry chromatographic jar.
     To make sure that the environment in the jar is saturated with solvent vapours, a paper soaked
    in the mobile phase is applied to the walls.
     Add the mobile phase to the jar and close it.
     Maintain equilibrium.
     Mark the baseline on the adsorbent.
     Apply sample to TLC plate with the help of a capillary tube and allow it to dry.
     Put the plates in the jar and close it.
     Wait until the solvent moves from the baseline.
     Take out the TLC plate and dry it.
    Adsorption Chromatography Experiment
    (TLC):
    Process of Adsorption Chromatography:

    Completed in two phases:


    1.Stationary Phase:
    •Initial stage
    •Forces are employed to remove solutes from adsorbents.
    2. Mobile Phase:
    •Liquid or Gas is used as a simulator.
    •Internal forces remove solutes from the adsorbents. 
    Types of Adsorption Chromatography:

    1.Column Chromatography

    2.Thin-Layer Chromatography

    3.Gas-Solid Chromatography 
    1. Column Chromatography:

     Column is packed with solid and is served for the stationary phase.
     Mobile phase(liquid) is run through the column.
     The component with the absorption of stronger will appear at the
    top of the column.
     Simultaneously, the compounds moving fast will be absorbed
    weakly and will end up concentrating at the bottom of the beaker.
    2. Thin-Layer Chromatography:

     Process of studying the solutions or mixtures by understanding the


    absorption capabilities of liquids in them.
     Carried out in three steps:
    o Spotting the difference between the mixture.
    o Studying the development undergone in phases.
    o Visualize the separation of absorption levels.
    3. Gas-Solid Chromatography:

     Process of separating a mixture with the use of gases.


     Inert
    gases like helium or nitrogen are charged as the
    mobile phase, the solid is furnished by a stationary phase.
    Applications of Adsorption
    Chromatography:

     Seperatingunsaturated hydrocarbons from a mixture of


    organic compounds.
     Identification of carbohydrates.
     Seperating diastereomers.
     Seperating & isolating lipids, steroids and fats. 
    REFERENCES:

     https://byjus.com/chemistry/adsorption-chromatography/ (BYJU)
     https://collegedunia.com/exams/adsorption-chromatography-types-principle-proc
    edure-application-chemistry-articleid-1677
     https://www.vedantu.com/chemistry/adsorption-chromatography
     Separation techniques: Chromatography (nih.gov)
     Chromatography - an overview | ScienceDirect Topics

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