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    Teknik Pemeriksaan Molekuler TBC

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    Hani Hibatullah Konversi Semester 1 SKM
    This webinar discusses molecular tuberculosis testing using the GeneXpert platform. The speaker provides their credentials and an outline of the webinar topics, which include the global epidemiology of TB, molecular diagnostic policies and guidelines, Mycobacterium tuberculosis characteristics, and the GeneXpert technology and testing procedure. Sample requirements, preparation techniques, and result interpretation for the GeneXpert MTB/RIF assay are explained in detail.

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    Teknik Pemeriksaan Molekuler TBC

    Uploaded by

    Hani Hibatullah Konversi Semester 1 SKM
    64 views28 pages
    This webinar discusses molecular tuberculosis testing using the GeneXpert platform. The speaker provides their credentials and an outline of the webinar topics, which include the global epidemiology of TB, molecular diagnostic policies and guidelines, Mycobacterium tuberculosis characteristics, and the GeneXpert technology and testing procedure. Sample requirements, preparation techniques, and result interpretation for the GeneXpert MTB/RIF assay are explained in detail.

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    This webinar discusses molecular tuberculosis testing using the GeneXpert platform. The speaker provides their credentials and an outline of the webinar topics, which include the global epidemiology of TB, molecular diagnostic policies and guidelines, Mycobacterium tuberculosis characteristics, and the GeneXpert technology and testing procedure. Sample requirements, preparation techniques, and result interpretation for the GeneXpert MTB/RIF assay are explained in detail.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    64 views28 pages

    Teknik Pemeriksaan Molekuler TBC

    Uploaded by

    Hani Hibatullah Konversi Semester 1 SKM
    This webinar discusses molecular tuberculosis testing using the GeneXpert platform. The speaker provides their credentials and an outline of the webinar topics, which include the global epidemiology of TB, molecular diagnostic policies and guidelines, Mycobacterium tuberculosis characteristics, and the GeneXpert technology and testing procedure. Sample requirements, preparation techniques, and result interpretation for the GeneXpert MTB/RIF assay are explained in detail.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 28

    WEBINAR HIMAJU TLM POLTEKKES KEMENKES MANADO

    Teknik Pemeriksaan
    Molekuler Tuberkulosis

    Steldy Runtuwene Lantaka,Amd.AK, S.Si


    CURRICULUM VITAE

    Nama : STELDY RUNTUWENE LANTAKA


    TTL : KARATUNG, 11 NOVEMBER 1992
    Agama : Kristen Protestan
    Pekerjaan : ASN
    Riwayat Pekerjaan : Staf Laboratorium Klinik RSUP Prof. DR. R. D. Kandou Manado (2016 –
    sekarang)
    Tenaga pengajar Prodi D3 Teknologi Laboratorium Medik Poltekkes Kemenkes
    Manado (2019 – sekarang)
    Pendidikan : S1 Analis Medis (Sekolah Tinggi Analis Bakti Asih Bandung-2016)
    DIII Analis Kesehatan (Poltekkes Kemenkes Manado-2014)
    Riwayat Organisasi : Sekretaris DPC PATELKI Manado (2017 – 2019)
      Sekretaris DPW PATELKI Sulut (2018 – Sekarang)
    Verifikator P2KB Profesi ATLM Wilayah Sulawesi Utara (2019 – sekarang)
    Outline
    01 Epidemiologi Global TB

    02 Kebijakan Pemeriksaan
    Molekuler TB-MDTB

    03 Mycobacterum tuberkulosis

    04 GeneXpert Technology &


    procedure
    WHO-Global Tuberculosis Report 2019

    Indonesia

    Content Here
    Get a modern PowerPoint Presentation that is beautifully designed.
    hope and I believe that this Template will your Time. I hope and I
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    can simply impress your audience and add a unique zing and appeal
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    WHO-Global Tuberculosis Report 2019
    WHO RECOMMENDED – LABORATORY DIAGNOSTIC
    Diagnostic Drugs Susceptibility
    Testing
    Direct Microscopic for AFB
    ZN staining, auramine-rhodamine stains PHENOTYPIC
    Imunoserology assay Culture TB
    Lipoarabinomannan urine test (LF-ELISA) for TB-HIV Liquid medium (MGIT, BACTEC, BacT ALERT, etc)
    Interferon Gamma Release (IGRA) for TB Laten Solid (Egg base/LJ, Agar &H10/7H11)
    Diagnostic
    Technology
    Culture TB
    Liquid medium (MGIT, BACTEC, BacT ALERT, etc)
    Solid (Egg base/LJ, Agar &H10/7H11)
    GENOTYPIC
    NAATs NAATs
    X-pert MTB/Rif X-pert MTB/Rif
    Line Probe Assay
    Line Probe Assay
    Loop-mediated isothermal amplification (TB-LAMP)s
    Loop-mediated isothermal amplification (TB-LAMP)s

    High specificity. Better sensitivity than microscopy. Fast results. Allows identification
    and investigation of genetic resistance patterns, biosafety precautions similar to
    those needed for direct smear microscopy (BSL 2)
    Your Picture Here

    You can simply impress your audience and add


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    Presentation that is beautifully designed. You
    can simply impress your audience and add a
    unique zing and appeal to your Presentations.
    Easy to change colors, photos and Text. Get a
    modern PowerPoint Presentation that is
    beautifully designed.

    Modern
    Portfolio
    Presentation
    Xpert MTB/RI

    Your Picture Here


    Your Picture Here

    Kebijakan
    Pemerintah
    Indonesia
    Mycobacterium tuberculosis
    • Penyebab penyakit infeksi
    Tuberkulosis
    • Berbentuk sel Y Resistensi terhadap rifampisin pada M. tuberculosis
    • Bentuk dan Ukuran bervariasi : sebagian besar (> 95%) disebabkan mutasi gen rpoß
    oval pendek 0,5 – 1 um, batang 2-4 yang menyandi RNA polymerase subunit ß pada
    um, filament panjang 6 -7 um 81 hot spot region
    • Dinding sel (outer; protein-lipid,
    inner; peptidoglycan (PG),
    arabinogalactan(AG), and mycolic
    acid (MA)

    Farha et.al, Genomic analysis identifies targets of convergent positive selection in drug-resistant Mycobacterium
    tuberculosis
    Nature genetics article
    GeneXpert Technology
    & Xpert MTB-RIF Procedure
    Gene Xpert
    Platform Design
    Teknologi Gene Xpert

    Teknik Real-Time PCR dengan pembacaan


    Fluoresensi.

    Tidak ada kontak antara sampel dengan instrumen

    Teknologi Ultrasonic Horn untuk melisiskan sel

    Sistem Internal Kontrol terintegrasi dalam cartridge

    Reduksi data dan interpretasi hasil otomatis


    Komponen Instrumen
    GeneXpert
    Modul GeneXpert
    Catridge
    GeneXpert
    Spesimen Pemeriksaan

    SPUTUM
    01 Jlh specimen yg digunakan sebanyak 2 (S-S, S-P).
    • Satu utk pemeriksaan
    40% 80% 60% 50% • Saru disimpan sementara jika specimen selumnya
    memberikan hasil invalid, Rif Intermediet, Rif resisten
    pd bukan tersangka RO

    SPESIMEN EXTRA PARU


    02 Cairan CSF, jaringan biopsi, bilasan lambung, aspirasi
    cairan lambung,feses.

    Tidak dapat digunakan


    03 Cairan pleura, urine, pus dan darah.
    Preparasi Sampel
    Teknik Gene Xpert MTB-RIF
    Teknik Pemeriksaan
    GeneXpert MTB-RIF
    MTB-RIF Assay Design
    Interpretasi Hasil
    HASIL
    PEMERIKS
    MTB Detected – Rif Resistance Detected
    AAN

    MTB Not Detected


    Thank you
    Semoga Bermanfaat

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