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    Laboratory Diagnosis of Fungi 2021

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    Mr.Fantasthicc
    This document discusses the laboratory diagnosis of fungal infections. It covers general properties of fungi and differences between fungi and bacteria. It describes morphological forms of fungi including yeasts and molds. It also discusses dermatophytes, reproduction, and classification of fungi. The document outlines methods for laboratory diagnosis of fungal infections including microscopy, cultivation, biochemical characteristics, and genomic and proteomic tests. It provides information on growth characteristics, identification, and rapid tests used to diagnose fungal infections in the laboratory.

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    Laboratory Diagnosis of Fungi 2021

    Uploaded by

    Mr.Fantasthicc
    29 views68 pages
    This document discusses the laboratory diagnosis of fungal infections. It covers general properties of fungi and differences between fungi and bacteria. It describes morphological forms of fungi including yeasts and molds. It also discusses dermatophytes, reproduction, and classification of fungi. The document outlines methods for laboratory diagnosis of fungal infections including microscopy, cultivation, biochemical characteristics, and genomic and proteomic tests. It provides information on growth characteristics, identification, and rapid tests used to diagnose fungal infections in the laboratory.

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    Laboratory diagnosis of fungi 2021

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    This document discusses the laboratory diagnosis of fungal infections. It covers general properties of fungi and differences between fungi and bacteria. It describes morphological forms of fungi including yeasts and molds. It also discusses dermatophytes, reproduction, and classification of fungi. The document outlines methods for laboratory diagnosis of fungal infections including microscopy, cultivation, biochemical characteristics, and genomic and proteomic tests. It provides information on growth characteristics, identification, and rapid tests used to diagnose fungal infections in the laboratory.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    29 views68 pages

    Laboratory Diagnosis of Fungi 2021

    Uploaded by

    Mr.Fantasthicc
    This document discusses the laboratory diagnosis of fungal infections. It covers general properties of fungi and differences between fungi and bacteria. It describes morphological forms of fungi including yeasts and molds. It also discusses dermatophytes, reproduction, and classification of fungi. The document outlines methods for laboratory diagnosis of fungal infections including microscopy, cultivation, biochemical characteristics, and genomic and proteomic tests. It provides information on growth characteristics, identification, and rapid tests used to diagnose fungal infections in the laboratory.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 68

    Introduction to lab diagnosis of fungi

    General Properties of Fungi


    Are fungi closer to Plants or Animals
    Differences between fungi and
    bacteria
    Yeast vs. Molds
    Nutritional Adaptations of Fungi
    Two morphological forms
    Yeast
    Yeast
    Yeast
    Mould
    Mould
    Mould
    Mould
    Mould
    Chlamydospores
    Chlamydospores
    Arthrospores

    Dermatophytes are a common label for a group of fungus of


     Arthrodermataceae that commonly causes skin disease in animals and
    humans.Traditionally, these anamorphic (asexual or imperfect fungi) mold
    genera are: Microsporum, Epidermophyton and Trichophyton.
    Reproduction
    Sexual spore

    ZYGOSPORES
    Asexual spores
    Morphological classification
    Morphological classification
    Morphological classification
    Phaeoid fungi
    Systemic classification
    Zygomecetes

    Rhizopus
    Ascomycetes
    Basidiomycetes
    Pathogenic classification
    Fungal diseases
    Classification of Mycoses
    Transmission
    Lab. dg of fungal infection
    • Microscopy
    • Cultivation
    • Biochemical characteristics
    • Genomic tests
    • Proteomic analysis

    https://youtu.be/YHY7hob8DMI
    Laboratory diagnosis of fungal infections, 17 min.
    Lab diagnosis of fungal infections
    Lab diagnosis of fungal infections
    Lab diagnosis of fungal infections
    Lab diagnosis of fungal infections
    Lab diagnosis of fungal infections
    Growth characteristics useful for
    identification of fungi
    Identification
    https://youtu.be/ot-4CB1y4W4

    https://youtu.be/OMIF1Elr9i4

    https://youtu.be/duNktChMxWw

    https://youtu.be/vNqioD6oN_U
    Rapid tests
    AUXACOLOR
    PRINCIPLE OF THE TEST

    The AUXACOLORTM 2 kit is an identification system based on the principle of sugar


    assimilation. The growth of yeasts is visualised by the colour change of a pH indicator.
    The kit also includes 3 enzyme tests, including a test for detection of the
    phenoloxidase activity of Cryptococcus neoformans
    The Auxacolor kit comprises
    • a negative control to facilitate interpretation of the sugar assimilation
    results (blue well).
    • 13 sugar assimilation results, corresponding to the following sugars:
    - glucose (GLU.) : positive control
    - maltose (MAL.) - cellobiose (CEL.)
    - sucrose (SAC.) - trehalose (TRE.)
    - galactose (GAL.) - adonitol (ADO.)
    - lactose (LAC.) - melezitose (MEL.)
    - raffinose (RAF.) - xylose (XYL.)
    - inositol (INO.) - arabinose (ARA.

    Reading of the results


    The definitive reading should be taken after 48 hours. Even though a first reading after 24 hours may give a correct score and
    allow certain yeasts to be identified, definitive interpretation should be made after 48 hours.
    If a Cryptococcus infection is suspected, the definitive reading should be made after 72 hours given the slow growth of these
    microorganisms.
    Interpretation of results
    Guide for interpretation of results
    Interpretation of results
    Scoring methodology
    Final identification
    • based on a combination of the biochemical tests and complementary
    criteria (morphological and metabolic)
    Introduction
    Clinical features
    Oropharyngeal candidiasis
    2. Vulvovaginal (genital) candidiasis
    3. Invasive candidiasis (candidemia)
    Laboratory diagnosis
    Laboratory diagnosis
    Laboratory diagnosis
    Laboratory diagnosis
    Treatment

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