The Slow Death of Spontaneous Generation

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The Slow Death of Spontaneous

Generation
Spontaneous Generation
• What do you think this means?
+ =

Horse Water
Hair Snake
Francesco Redi (1626 – 1697)
“Founder of Experimental Biology” and first to
challenge the theory of Spontaneous Generation
• At the time of Francesco
Redi it was believed
that rotting meat gave
rise to maggots.
• What kind of experiment
do you think Redi
designed to prove that
maggots come from flies
that visit the rotting
meat?
• If you were Redi, what
procedure would your
procedure be?
(remember: He lived in
the 17th century!)
• What was the
independent variable in
Redi’s experiment?

• Type of lid on the


container
Lazzaro Spallanzani (1729 – 1799)
• One hundred years after Redi another
Italian scientist took on spontaneous
generation.
• Spallanzani experimented with
microorganisms in gravy.

• Where did the microorganisms in the


flask on the left come from?
• What is a limitation of this experiment
in disproving spontaneous
generation?
Louis Pasteur (1822 – 1895)
• This French scientist
contributed much of
what we know about
microorganisms and
disease.
• His experiments, among
many other important
things, disproved the
idea of spontaneous
generation.
Designing Pasteur’s Experiment
• Using beef broth
and glass flasks,
what scientific
design do you think
Pasteur used that
was different from
Spallanzani?
Remember: This is
what Spallanzani’s
experiment looked
like:
Designing Pasteur’s Experiment
• What was the purpose
of the “S” shaped tube
on the top of the flask?
• This shape allowed air
to continue to enter the
flask but prevented air-
borne microorganisms
from coming into
contact with the broth.
Parts of a controlled experiment:
• How many independent variables should be
tested for in an experiment at one time?
– Only one
• What part of an experiment has the
independent variable?
– The experimental group
• What part of an experiment does not have the
independent variable?
– The control group
Parts of a controlled experiment:
• What is the purpose of the control group?
– To use as a comparison, so you know what would
normally happen in the experiment if the
independent variable was not introduced
• In Pasteur’s experiment what was the control
group?
– The flask with the unbroken
tube
Parts of a controlled experiment:
• In Redi’s experiment what was the control
group?
– The jar open to the air
Parts of a controlled experiment:
• Pick one of the three experiments and list as
many controlled variables (constants) as you
can think of.
One Last Note:
• Louis Pasteur developed the process by which
food is heated to a high temperature for a
short amount of time and then cooled. What
is this process called?
• Pasteurization

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