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    Measurement Process Flow Chart (EXAMPLE: Nanotoxicity Assay)

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    yildirimonline
    The document outlines the measurement process flow chart for a nanotoxicity assay, which involves seeding cells, treating them with nanoparticles, incubating for 24 hours, removing the supernatant, treating with MTS reagents, and measuring absorbance at 490nm to determine cell toxicity response. It also provides templates for including measurement controls, constructing an Ishikawa diagram to identify variables that could affect results, specifying reference materials, and planning an inter-laboratory study to establish a standard test method.

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    Download as PPTX, PDF, TXT or read online from Scribd

    Measurement Process Flow Chart (EXAMPLE: Nanotoxicity Assay)

    Uploaded by

    yildirimonline
    19 views7 pages
    The document outlines the measurement process flow chart for a nanotoxicity assay, which involves seeding cells, treating them with nanoparticles, incubating for 24 hours, removing the supernatant, treating with MTS reagents, and measuring absorbance at 490nm to determine cell toxicity response. It also provides templates for including measurement controls, constructing an Ishikawa diagram to identify variables that could affect results, specifying reference materials, and planning an inter-laboratory study to establish a standard test method.

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    The document outlines the measurement process flow chart for a nanotoxicity assay, which involves seeding cells, treating them with nanoparticles, incubating for 24 hours, removing the supernatant, treating with MTS reagents, and measuring absorbance at 490nm to determine cell toxicity response. It also provides templates for including measurement controls, constructing an Ishikawa diagram to identify variables that could affect results, specifying reference materials, and planning an inter-laboratory study to establish a standard test method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    19 views7 pages

    Measurement Process Flow Chart (EXAMPLE: Nanotoxicity Assay)

    Uploaded by

    yildirimonline
    The document outlines the measurement process flow chart for a nanotoxicity assay, which involves seeding cells, treating them with nanoparticles, incubating for 24 hours, removing the supernatant, treating with MTS reagents, and measuring absorbance at 490nm to determine cell toxicity response. It also provides templates for including measurement controls, constructing an Ishikawa diagram to identify variables that could affect results, specifying reference materials, and planning an inter-laboratory study to establish a standard test method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 7

    Measurement Process Flow Chart

    (EXAMPLE: Nanotoxicity Assay)

    Treat with
    Add Remove
    Seed Cells Incubate 24 h MTS
    Nanoparticles Supernatant
    Reagents

    Measure
    Abs490
    Measurement Process Flow Chart (TEMPLATE)
    Measurement Controls (TEMPLATE)
    Control
    Variables/Factors Why an Issue? Experiment Reference Material?
    /Approach
    Cell response varies due to
    Add cadmium sulfate to cells to
    EXAMPLE: Nanotoxicity assay: many factors (passage,
    establish a reproducible “toxic” Cadmium sulfate
    Variable: Cell toxicity response incorrect seeding, variability in
    response in the assay
    medium, contamination, etc.)
    Ishikawa Diagram (EXAMPLE: Nanotoxicity Assay)

    Rösslein M, Elliott JT, Salit M, Petersen EJ, Hirsch C, Krug HF, Wick P. Use of Cause-and-Effect
    Analysis to Design a High-Quality Nanocytotoxicology Assay. Chem Res Toxicol. 2015, in press.
    Ishikawa Diagram (TEMPLATE)

    Cause Effect

    Problem
    Reference Materials (TEMPLATE)
    1
    What reference materials do you employ in your assay. How & why do you use them?

    2
    What needs do you see for new or better reference materials. How would you use them? Why?

    What properties must be specified for the proposed new reference materials?
    Inter-Laboratory Studies (TEMPLATE)
    1 What is the goal of the inter-laboratory study? Tech. 4
    What process controls should there be?
    transfer? Establishing a standard test method?
    1. 4.

    2. 5.
    2 Participating Labs
    1. 4. 3. 6.
    2. 5.
    3. 6.
    Others? Others?

    3 How will the How will Parameters that Materials to Materials provided What level of
    SOP be operators be must be tested for distribute to all by the agreement is
    established? trained? sensitivity? participating labs? participating labs? adequate? When
    are we done?

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