Assignment: Gollis University

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GOLLIS UNIVERSITY
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.1 Louis Pasteur………………………………………………………………………………….1
• 2 Contribution of Louis Pasteur …………………………………………………... 2

• 3bioterrorism…………………………………………………………………………………..3
• 4what does bioterrorism work…………………………………………………………4
• 5what couses bioterrorism………………………………………………………………5
Louis Pasteur

Louis Pasteur is well known for his contribution in


microbiology such as development of antrax vaccination,
microbial fermentation, germ theory of disease and
pasteurization.
Pasteur was a famous French biologist, microbiologist, and
chemist.
He was born on December 27, 1822, in Dole, located in the
Jura region of France.
Pasteur died at the age of 72 on September 28, 1895
Contribution of Louis Pasteur
• Spontaneous generation: At the earlier people were believed in
spontaneous generation theory, that living cells were generated from
nonliving cells. Pasteur believed in biogenesis theory means, living cells
were generated from living material rather than nonliving materials.
Pasteur disproved the spontaneous generation theory by his Swan Necked
Flask experiment.
• Crystallography: In earlier Pasteur was a chemist. During his doctoral
dissertation, he work as a laboratory assistant at the École Normale.
During this time he investigates that the ability of certain crystals or
solutions to rotate plane-polarized light clockwise or counterclockwise,
that is, to exhibit “optical activity.” Pasteur was able to exhibit that in some
cases this activity related to the shape of the crystals of a compound.
• Fermentation:  In the earliest time people believe that the fermentation
process is conducted by a series of chemical reactions. In 1854, while he was
working at Lille, Pasteur investigate fermentation processes and he noticed
that the fermentation processes are carried out by a living microorganism. 
• Pasteurization: To prevent the souring of wine or to fight against the
“diseases” of wine he discovered the Pasteurization Processes. He noticed
that the spoiling beverages, such as beer, wine, and milk is caused by some
unwanted microorganisms. So, he invented a process in which the
microorganisms could be destroyed by heating the wine between 60° and
100°C temperature.
• Germ Theory of Diseases: Pasteur and a minority of other scientists believed
that diseases were caused by microorganisms—germ theory. Later he
proved this theory by investigating the causes of silkworm disease.
• Anthrax vaccine:  In 1881, Louis Pasteurpulically announced that he
successfully discovered the vaccine of anthrax.
• Rabies: Louis Pasteur first produced the vaccine of rabies virus. He
produced it by growing it in rabbits, and then weakening it by drying the
affected nerve tissue.
• Chicken cholera: In 1879, Pasteur inoculate the culture of chicken cholera
with a healthy chicken to infect them, but the chickens were survived.
Later he inoculated this chicken with a virulent strain of the same culture,
this time he noticed that they were immune to it. From this experiment
he discovered that he could not infect them, even with fresh bacteria; the
weakened bacteria had caused the chickens to become immune to the
disease, though they had caused only mild symptoms.
The Swan Necked Flask experiment of  Louis Pasteur
• He designed several bottles with S-curved necks or also known as gooseneck flask, which
were oriented downward so gravity would prevent access by airborne foreign materials.
• Then he placed nutrient-enriched broth in one goose-neck bottle.
• Then he boiled the broth inside the bottle and kept it for one year in sealed condition.
• After one year he observed no life in the jar.
• Then he broke the top portion of the goose-neck bottle and directly exposed to the air.
• After a few days, heHe noticed that no life forms were observed in the broth until this
obstacle was removed from the bottle, because air born particles and dust were trapped
in the S-shaped neck of the bottle.
• He confirmed that the contamination came from life-forms in the air. From this
experiment, he disproves the spontaneous generation theory observed that the broth has
contains life.
BIOTERRORISM

• Bioterrorism is terrorism involving the intentional release or


dissemination of biological agents. These agents are bacteria, viruses,
insects, fungi, or toxins, and may be in a naturally occurring or a
human-modified form, in much the same way in biological warfare.
Further, modern agribusiness is vulnerable to anti-agricultural attacks
by terrorists, and such attacks can seriously damage economy as well
as consumer confidence. The later destructive activity is called
agrobioterrorism and is subtype of agro-terrorism.

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