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    Dna Extraction From Cheek Cells

    Uploaded by

    John Lhoyd Tuazon
    This document provides instructions for extracting DNA from cheek cells at home. It involves collecting cheek cells by swishing a saline solution in the mouth, then lysing the cells using dish soap. DNA is precipitated by adding cold ethanol, which should cause white strands to form at the interface between the ethanol and cell solution. Notes and photos should be taken to document the experiment.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Dna Extraction From Cheek Cells

    Uploaded by

    John Lhoyd Tuazon
    141 views9 pages
    This document provides instructions for extracting DNA from cheek cells at home. It involves collecting cheek cells by swishing a saline solution in the mouth, then lysing the cells using dish soap. DNA is precipitated by adding cold ethanol, which should cause white strands to form at the interface between the ethanol and cell solution. Notes and photos should be taken to document the experiment.

    Original Description:

    From my biology teacher that will really helps you By Mrs.Rolda

    Original Title

    DNA-EXTRACTION-FROM-CHEEK-CELL

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    This document provides instructions for extracting DNA from cheek cells at home. It involves collecting cheek cells by swishing a saline solution in the mouth, then lysing the cells using dish soap. DNA is precipitated by adding cold ethanol, which should cause white strands to form at the interface between the ethanol and cell solution. Notes and photos should be taken to document the experiment.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    141 views9 pages

    Dna Extraction From Cheek Cells

    Uploaded by

    John Lhoyd Tuazon
    This document provides instructions for extracting DNA from cheek cells at home. It involves collecting cheek cells by swishing a saline solution in the mouth, then lysing the cells using dish soap. DNA is precipitated by adding cold ethanol, which should cause white strands to form at the interface between the ethanol and cell solution. Notes and photos should be taken to document the experiment.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 9

    DNA EXTRACTION

    FROM CHEEK
    CELLS
    MATERIALS
    Clean Plastic cups (clear)

    Table salt

    Distilled water

    Dishwashing liquid

    Ethyl Alcohol, Ice cold

    Test tube /vial / any small clear  container


    PREPARE 1. SALINE SOLUTION- Add half pinch salt to
    a glass full of distilled water
    THE
    2. DISHWASHING LIQUID SOLUTION- 1 par t

    FOLLOWING dishwashing liquid and 3 parts water  

    3. Ice cold ethanol / alcohol- refrigerate

    SOLUTION
    Part 1: CELL HARVESTING

    Do not eat or drink 15 minutes before sample collection.


    • Pour saline solution into a clean plastic drinking cup until half full.
    • Pour the saline solution into your mouth and “swish” the water around  between
    your cheeks and gums for 60 seconds.
    • You may “scrape” the inside of your cheeks with your teeth to help slough  off
    cheek cells to increase DNA yield.
    • Spit all of  the water back into the empty plastic cup. You may transf er the
    solution into a test tube or vial if you have one.

    • Your plastic cup now contains cells from your cheeks, called buccal
    cells.  These cells still contain an intact nucleus, where the DNA is
    found.
    Part 2: CELL LYSIS

    • Add 40 drops dishwashing liquid solution


    to the collected saline solution 
    • Stopper the test tube , when using
    different container mix by inverting it 
    gently
    • Do not shake the test tube.  Excess
    shaking can break the long strands
    of DNA, making it harder to see and
    collect when precipitated.​
    Part 3: PRECIPITATION

    • Holding the test tube at a slight angle,  carefully  add


    25mL of the ice-cold ethyl alcohol down the side of the
    test tube so it forms a layer over the  cheek cell
    mixture.  Do not mix the water and ethanol layers.
    • Hold the test tube /vial upright for 60 seconds and
    obser ve what happens at the  inter face between the
    ethyl alcohol and the cheek cell solution.
    Successfully
     extracted
    DNA
    DOCUMENT YOUR
    EXPERIMENT BY TAKING
    PHOTOGRAPHS AND
    NOTES OF YOUR
    OBSERVATIONS
    • Buckingham, L. (2012). DNA, DNA
    extraction methods.
    In MolecularDiagnostics: Fundamentals,
    Methods, and Clinical Applications  (2 nd
    ed., pp. 2-5, 70-77). Philadelphia, PA: F.A.
    Davis Company. 
    • Flinn Scientifi c DNA Isolation Kit, Catalog
    No. FB2065, 2012. 
    references • Pearson Education. (n.d.). The
    Biology Place. In Pearson
    Education.Retrieved September  13 2016,
    from http://www.phschool.com /
    science /biology_place /biocoach /cells /com
    mon.html
    • Some photographs from LIZ BRANDON

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