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BBL741 Protein Science and Engineering: by Prof. S. Mishra Dept. of Biochemical Engineering and Biotechnology

The document provides an outline for the course BBL741 Protein Science and Engineering. It includes 4 modules that cover topics such as structural families of proteins, protein folding and engineering, protein-ligand interaction, and protein design. There will be 42 total lecture hours over 14 weeks. Students will be evaluated based on minor tests, quizzes, a term paper, seminar, class participation, and a major test. The course will take a multidisciplinary approach and use computational techniques and molecular modeling to study protein structure, function, and engineering.

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Sparsh Negi
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49 views10 pages

BBL741 Protein Science and Engineering: by Prof. S. Mishra Dept. of Biochemical Engineering and Biotechnology

The document provides an outline for the course BBL741 Protein Science and Engineering. It includes 4 modules that cover topics such as structural families of proteins, protein folding and engineering, protein-ligand interaction, and protein design. There will be 42 total lecture hours over 14 weeks. Students will be evaluated based on minor tests, quizzes, a term paper, seminar, class participation, and a major test. The course will take a multidisciplinary approach and use computational techniques and molecular modeling to study protein structure, function, and engineering.

Uploaded by

Sparsh Negi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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BBL741 Protein Science and

Engineering
1st semester 2020-2021
By
Prof. S. Mishra
Dept. of Biochemical Engineering and Biotechnology
Lecture 1-Introduction
Course outline
Module 1:Structural families Module 3: Protein engineering
Amino acids, their chemistry and their Strategies for protein engineering-random and site-
conformational accessibility, Ramachandran plot directed
Motifs of protein structures and their packing Role of low-fidelity enzymes
Schematic and topology diagrams Gene shuffling and directed evolution of proteins
Families of protein structures-alpha, alpha/beta, Protein backbone changes
beta, small etc. and protein databases All topics will deal with case studies

Module 2:Protein folding and assembly Module 4: Modelling protein-ligand interaction


Protein folding pathways in prokaryotes and eukaryotes and Protein design
Folding of single and multidomain proteins Homology modelling and modelling of protein-ligand
interaction
Inclusion bodies and recovery of active proteins
Structure based drug design and case studies
Osmolyte assisted protein folding
Rational protein design
Structure of chaperones and their role in protein folding
Distribution of Lectures
Lecture Outline(with topics and number of lectures)  
Module Topic No. of
no. hours
 1 Introduction, Structure of amino acids and their conformations 4
 1 Motifs of protein structure and their packing 3
 1 Structural families of proteins (alpha, beta, alpha/beta etc.) 5
 2 Protein folding and assembly theories 4
 2 Protein folding pathways-single and multidomain proteins, Recovery of proteins from 5
inclusion bodies
 2 Chaperone assisted protein folding 2
 3 Strategies for protein engineering (including SCHEMA) 3
 3 Case-studies (enzymes, antibodies, back-bone changes with relevance to pharma 5
sector)
 3 Drug-protein interaction and design 4
 4 Rational protein design 4
 4 Directed evolution of proteins 3
42
References
Introduction to Protein structure, 2nd Ed. Branden and Tooze, Garland,
N.Y.
Biochemistry, 4th Ed. (2011) D.Voet and J.G. Voet, Wiley N.Y.
Papers from Journals
E-resources
Evaluation policy
BBL741 - Protein Science and Engineering (SM)
(Total number of credits = 3) (LTP 3-0-0)

Evaluation Component Marks distribution


Minor Test 1 25
Minor Test 2 0
Quiz 1 10
Quiz 2 0
Term Paper 15
Seminar 10
Participation in class discussion-feedback and your
posting regular questions 10
PMT 70
Major Test 30
GRAND TOTAL 100
Lecture-1 outline
• Multiple disciplines
• Diversity of protein functions
• Meaning of structural families
• Basis for structure formation-nature and attributes of individual
amino acids
• Properties of the peptide bond and significance of Ramachandran Plot
Protein engineering-a multidisciplinary area
Computational
Protein Molecular techniques
crystallographyModeling Predictions
New Model
3D Structure
Properties
Crystals
Cell Protein Mutagenesis

Purification
Sequence Expression

Oligonucleotide
Cloning
Synthesis Molecular
Biochemistry
Biology
Protein-encoding gene functions in Caenorhabditis elegans
(common worm)
Proteins as receptors
Proteins as structural
components
1.Structural change,
so physical functions
are lost (Alzeimer’s).
2.Change in shape-
Creutzfeld-disease and
encephalopathies.
3. Conversion to long
chains-amyloidosis

Enzymes and protein receptors are usual


targets of drugs either to restore function or to
destroy infectious agents or cancers.

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