Scribd
Upload
90 views35 pages

Laboratory Safety: Course Instructor: Manam Walait

The document outlines various laboratory safety rules and procedures that students must follow in a microbiology lab, including wearing protective clothing and equipment, proper handling of chemicals and biological materials, safety protocols for equipment use, and procedures for disposal of waste and reporting accidents. It also describes different biological safety levels based on the pathogenic risk of organisms being studied and the corresponding safety equipment and facility requirements.

Uploaded by

Jawad
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
90 views35 pages

Laboratory Safety: Course Instructor: Manam Walait

The document outlines various laboratory safety rules and procedures that students must follow in a microbiology lab, including wearing protective clothing and equipment, proper handling of chemicals and biological materials, safety protocols for equipment use, and procedures for disposal of waste and reporting accidents. It also describes different biological safety levels based on the pathogenic risk of organisms being studied and the corresponding safety equipment and facility requirements.

Uploaded by

Jawad
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 35

Lab.

Laboratory safety

Course Instructor: Manam Walait


Introduction
• Laboratory safety rules are a major aspect
of every microbiology lab.

• Each student in microbiology laboratory


must follow specific safety rules and
procedures.
Why is Lab Safety Important?

• Lab safety rules and


symbols are needed so
that students do not injure
themselves or their
classmates.
Lab Safety Rules
Lab Safety Rules
1-Wear protective clothing .
Protective clothes
o Gloves are essential.

o Lab coats are


required.

o Safety
glasses
( goggles) may be
required to avoid
splashes.
Lab Safety Rules
2-Laboratory personnel should not wear sandals
Lab Safety Rules
Do NOT Wear:

• Jewelry

• Loose or Baggy clothing


Lab Safety Rules
3-Avoid touching objects (e.g., pencils, cell phones,
door handles) while wearing gloves.
4-Pencils, labels, or any other materials should never be
placed in your mouth.
5-Caution must be taken when using gas burners. Be
sure gas burners are turned off when finished.
Lab Safety Rules
6-Long hair must be tied back or covered to minimize
fire hazard or contamination of experiments.
Lab Safety Rules
7- Do not eat food or drink water in the lab.
do not use lab glassware as food or water containers.
Lab Safety Rules
8- Protect your hands safety:
- wash hands after every lab.
- Handle glassware, sharp tools and heated containers carefully.
Lab Safety Rules
9 electrical safety:
- unplug electrical equipment after use.
- keep all electrical cords and wires away from water .
Lab Safety Rules
10- Chemical safety:
-never touch, taste or smell a chemical unless instructedto do
so.
- never mix chemicals unless instructed to do so.
-keep lids on chemical containers when not in use.
Lab Safety Rules
11 Do not take any cultures out of the
lab for any reason.
• All cultures should be handled as
potentially pathogenic.
• Liquid cultures must always be
kept in a test tube rack.
Lab Safety Rules
12- Do not engage in practical jokes or horseplay in the
lab.
Lab Safety Rules
13-Keep nonessential books and clothing far away from
your work area.
Lab Safety Rules
14-Wipe the bench tops down with disinfectant both before you
begin your work and after you have completed your work.
Lab Safety Rules
15- Dispose of waste products according to instructions.
Lab Safety Rules
16-Report all accidents, no matter how minor, to
your supervisor!!
Lab Safety Equipment
Lab Safety Equipment
• Safety shower

• Eye wash
Lab Safety Equipment

• Fire Blanket

• Fire Extinguisher
Lab Safety Symbols
Lab Safety Symbols
• They alert about the possible dangers in the lab.
Biological Safety Levels
Biological Safety Levels

1. BSL1
2. BSL2
3. BSL3
4. BSL4
BSL-1

– Not known to cause disease in healthy


adult humans
• Safety equipment
– Minimal requirements
• Facilities
– Open bench top
BSL-2

– Associated with mild to moderate


disease in humans
• Safety equipment
– Biological Safety Cabinet and
personal protective equipment as
needed.
• Facilities
– BSL-1 plus the availability of a
mechanism for decontamination.
BSL-3

– Associated with serious or


potentially lethal disease in
humans
• Safety equipment
– Biological Safety Cabinet and
personal protective equipment
required.
• Facilities
– BSL-2 with self-closing
double door access and
single-pass negative
directional airflow.
BSL-4

– Associated with high risk of


life-threatening disease in
humans and/or animals
• Safety equipment
– Biological Safety Cabinet
– Full-body air-supplied,
positive pressure personnel
suit
• Facilities
– BSL-3 plus dedicated air and
exhaust, decontamination
procedures for exit, separate
building, etc.

You might also like