Guidelines For Protocol and Dissertation Writing
Guidelines For Protocol and Dissertation Writing
Dissertation Writing
Introductions consists of
1.1 Background information & statement of the problem
1.2 Justification
1.1 Background information & statement of the problem
Background information
- It should be brief.
- State clearly the questions that you try to answer in the study.
Contents
decided to study.
What is the problem
included.
expectation.
or test.
Why do you study the research problem?
What are the contributions that will be made by the present
study?
Hypothesis
Type I Error
-Reject the null hypothesis(Ho) when it(Ho) is actually true.
Type II Error
- Fail to reject the null hypothesis when it is actually false.
and contrasted
theoretical issues
A. Informative
B. Evaluative
C. integrative
D. Organizing
Writing style
evaluate past work in your own words, don’t just copy and paste.
literature review
in
general terms and should be closely related to the statement of the problem.
General objectives
general, the more specifically the objectives are stated, the easier it will be to
Specific
Measurable
Achievable
Relevant
Time bound
Specific
Objectives have to state clearly, concisely and specifically what is to be
achieved at the end of the study
Measurable
Target & indicators have to be spelled out from the beginning so that it
is measurable at the end
Achievable
Your research must be achievable / doable within the allotted resources
& time frame.
Relevant
The study & its activities have to be relevant to the main goal (title), and
research method or study design.
Time bound
Mention the time frame within which the research has to be completed.
Objectives:
- Should be clear, precise and unambiguous statements, listed numerically
- Should be beginning with “To” and followed by action verbs or action-
oriented words
- Followed by specific and measurable variables and time, place and
person phrase
To
show, understand
Answer, carry out, define. Draw, establish, label, list, name, outline,
recognize, state
study, summarize
No value verbs and low value verbs must be avoided. If possible, try to
use high value verbs. Medium value verbs can be used if no better verb
can be identified.
Selected Verb should be relevant to research design.
• “explore”, “fine out” and “determine” used in descriptive study.
• “to compare or correlate or find out” used in analytic study.
• “to prove the hypothesis” in clinical trial or experimental study.
Examples of objectives
• To find out (action verb) the maximal acid output level (measure
variable) after vagotomy in chronic duodenal ulcer cases (person phrase)
admitted to the Surgical ward of Magway Teaching Hospital (place
phrase)
• To determine (action verb) the cause of death (measurable variable) in
closed abdominal injury cases (person phrase) admitted to the Surgical
ward of MRGH (place phrase)
5. RESEARCH METHODOLOGY
5.1 Study Design
Investigator assigns exposure of the study
Yes No
Yes No Yes No
Case Series:
Case series and case reports are potential ways to suggest an
association. They report the findings on a case by case basis.
(b) Analytical Study( compare the effect of an exposure)
Cross-sectional comparative studies:
Focus on comparing as well as describing groups.
E.g. a survey on Rheumatoid arthritis patients may wish to establish:
- The percentage of bone erosion/ deformity in RA patients of
certain duration
- Socio-economic, physical, treatment, functional variables that
influence the deformity
The researcher will not only describe these variables, but by comparing
deformed and non-deformed patients, he will try to determine
which socio-economic, physical or other independent variables
have contributed to erosion.
At the same time he has to watch out for confounding or intervening
variables. (analyzed by variable tests followed by multivariate tests
for selected significant variables)
Case-control studies
with another group, called a control group, where the problem is absent, to
RCT
to end of the year to compare raised ALT levels to determine the causal
A cohort study assembles a group of subjects and follows them over
relationship.
time. One follows these subjects to the development of an outcome.
the outcome.
Diagram of a cohort study
PRESENT FUTURE
(Prospective study, looking forward)
Problem present
exposed to
risk factor
Problem not present
compare
Problem present
Not exposed to
risk factors
Problem not present
Diagram of a case-control study
PAST PRESENT
(Retrospective study, looking backward)
compare
Sampling involves the selection of a number of study units from a defined population.
Study population – a group from which we want to draw a sample
eg. According to age, sex and residence
. Pretesting questionnaire
. Standardization of instrument
. Training of trainers
Methodology
Start from the very beginning. Describe all procedures in detail – how to
recruit objects, taking history, doing physical examination, how to collect
samples, detailed procedures of interventions or testing procedures of
samples, follow-up findings, interpretation of the data and data analysis. Use
flow-chart if necessary.
The Material and Methods section is very specific and very descriptive
and the methodology must be reproducible.
Variable
values.
(i) Continuous Scale – scaled continuously in range eg: weight in kg, height in cm
(ii) Ordinal Scale – rank the categories eg: income-high, middle, low
color (red, blue, green, etc), outcome of disease (recovery, chronic illness, death)
Other factors
(confounding variables)
Eg: Mother’s education => Malnutrition
Family income(CV)
Working definitation
Working definitions are very important for a study and you must state
Clearly before starting the real work.
Even for hypertension, there are different cut-off points WHO definition.
American definition (CDC), European difinition differs from each other
in some points. So you have to mention different approaches in literature
review. However, if the hypertension is one of the key variables in your study,
you have to state which definition you will use throughout your study in
Materials & Methods chapter.
Definitions for main variables expressed in aim and objectives must be
clearly spelled out. The end points to measure the fulfillment of objectives
must be clearly defined. Working definitions for inclusion criteria and
exclusion criteria are essential.
For protocol, in explanation of materials and methods, it should be
in future tense. E.g. the patients attending the rheumatology OPD, YGH will
be included in the study.
For the dissertations/thesis, materials and methods should be
explained in past tense because you had already completed the study and you
are now presenting your findings. Don’t just copy and paste the protocol to
\your dissertations/thesis. E.g. the patients who attended the rheumatology
OPD, YGH were included in the study.
Statistical Analysis
managed with means, standard deviation or standard errors and t-test. Once
the experiment goes into three or four groups, analysis of variance is used.
certainly in the materials and methods section and in the tabular or text results.
the tests being used, then consultation with statistician should be obtained.
CHOICE OF APPROPRIATE SIGNIFICANT TEST
No
Normal distribution Non-parametric Test
Yes
Parametric Test
Count Measure
Proportions Means
Z Chi2
Pop Sd Unpaired Paired ANOVA
Unpaired Paired
Z t Pop Sd Paired t
Z or X2 McNemar X2
Z t
Choice of basic statistical methods
Condition Non-parameteric test Patametric test
(Equivalent)
Before and after a single Wilcoxin Signed Rank One sample t-test
treatment in the same Test
idividuals
• Which variables will help you answer your research question and which is the
dependent variable?
Feasible (F)
-Adequate number of subjects
- Adequate technical expertise
- Affordable in time and money
- Manageable in scope
Novel (N)
-Confirms or refutes previous findings
- Extends previous findings
- Provides new findings
Ethical (E)
Relevant (R )
-To scientific knowledge
- To clinical and health policy
- To future research directions
Statistical Method
Mode – The number that occurs most often within a set of numbers.
Range – Difference between the highest and lowest values within a set of numbers
Normal Distribution (Gaussian Distribution)
The normal distribution is a symmetric distribution with no skew. The tails are exactly
the same.
- Mean = Median = Mode
- Centered
- Symmetrical
- Continuous
- Fixed Score Distribution
- Skewness = 0
- Unimodal => which means only one peak in distribution
- 50% lies above the mean and 50% lies below the mean
The "Bell Curve" is a Normal Distribution and the yellow histogram shows some data
that follows it closely, but not perfectly.
Eg: heights of people, errors in measurement, blood pressure
Standard Deviation
Standard deviation measure how far the data is spread from the mean.
A left-skewed distribution has a long left tail. Left-skewed distributions are also
called negatively-skewed distributions. That’s because there is a long tail in the
negative direction on the number line. The mean is also to the left of the peak.
Mean < Median < Mode
A right-skewed distribution has a long right tail. Right-skewed distributions are also
called positively-skewed distributions. That’s because there is a long tail in the
positive direction on the number line. The mean is also to the right of the peak.
Mode < Median < Mean
T-test (Student T-test)
T-test checks if two means(average) are reliably different from each other.
This test is used for comparing the means of two samples (or treatments), even if they
have different numbers of replicates.
P value is the probability that data in the sample could be produce by random.
Parametric test
Assumption are met
- Random independent samples
-Normal distribution
- Independence (one group did not influence the other)
- Homogeneity (the variances within each group are similar)
Parametric tests are more powerful, more likely to detect a difference that truly exists
and less likely than non-parametric test to make a type II error.
Non-parametric test
Assumption are met
-Random independent samples
- Non-normal distribution (skewed distribution)
Non-parametric tests are more conservative, less statistical power, less able to detect
a differences is truly there and more likely than parametric test to produce type II
error.
Comparing: Dependent variable Independent variable Parametric test Non-parametric test
(Dependent variable is
normally distributed)
The means of two Continuous/ scale Categorical/ nominal Independent t-test Mann-Whitney test
Independent groups
The means of 2 paired Continuous/ scale Time variable Paired t-test Wilcoxon signed rank
(matched) samples e.g: ( time 1= before, test
weight before and after time 2= after)
of a diet for one group of
subjects
The means of 3+ Continuous/ scale Categorical/ nominal One way ANOVA Kruskal-Wallis test
Independent groups
The 3+ measurements on Continuous/ scale Time variable Repeated measures Friedman test
the same subject ANOVA
Relationship between 2 Continuous/ scale Continuous/ scale Pearson’s Correlation Spearman’s Correlation
continuous variables Coefficient Coefficient (also use for
ordinal data)
Note: The table only shows the most common tests for simple analysis of data.
Ethical considerations
For a research project that should be considered ethical, the following
conditions should be fulfilled-
• The individual is invited to participate in the research, and that
participation is voluntary.
• The individual is free to refuse to participate and will be free to withdraw
from the research at any time without penalty or loss of benefits to which
he or she would otherwise be entitled.
• For controlled trials, the individual has been explained about the features
of the research design (e.g. Randomization, double-binding).
• The potential subject has been explained and understands following:
- the expected duration of participation
- any foreseeable risks, pain or discomfort, or inconvenience
- expected benefit to the subjects from participating of research
- confidentiality of records in which subjects are identified
Results
The result and discussion are very important major component of a research.
The result section answers the questions “What was found?”
It reports the results of the investigations described in the method sections.
It usually does not contain interpretation of data or statements that require
referencing.
It is composed of words (they tell the story), table (that summarize the
evidence), illustrations (that highlight the main findings), and statistics (that
support the statements).
Pay special attention to two pieces of general advice.
Firstly, keep the results section as brief and uncluttered as possible. The
reader must be able to see the wood for the tree.
Reports only the results that are relevant to the question and hypothesis.
Secondly, organize the presentation of results.
Lead the reader through the story by using a mixture of text, tables, and
illustrations.
The discussion section should contain an analysis of the result together with a
review of the relevant literature.
The important element is the interpretation of the result. It should also show
how previous studies relate to the present study and whether the hypothesis has been
proven or rejected.
In Discussion, don’t just copy the results, but also compare with previous local
and international studies which may agree or disagree with your own.
Data being reported should always be compared with landmark studies and if it
differs markedly from earlier ones, a plausible explanation should be provided.
If you find differing points from others or unexpected endings, discuss possible
explanation for non-uniformities.
Criticize the weakness/limitations and strength of your work and other studies in
terms of design, technique and results to find out the reason for the differences or
recommend further research on this topic.
For controversial findings, it is worthwhile to provide extra evidence as support.
It is not always possible or necessary to resolve conflicts.
Where no adequate or tangible explanation is possible, one can just say “there is
currently no explanation for the disparate results as found in this study until further
research can clarify the issue”.
Discuss all results including negative ones. Negative results need discussion and
not omission because they can raise specific research questions which can be used for
further research. Avoid being biased, speculative or unrealistic.
Never falsify(conceal the truth) your results. This is a breach of research as well
as professional ethics and do not think you will not be found out.
In the last paragraph of the Discussion section should outline the conclusions that
have been reached, and possibly indicate where the author intends to go from there.
Some authors put this paragraph in a separate section – Conclusions.
Conclusion
Link the conclusion with the goals of the study but avoid unqualified statement
and conclusions not adequately supported by the data.
State new hypothesis when warranted, but label them clearly such as:
• What is the strongest and most important statement that you can make from your
observations.
Citations in the body of your writing should give the author’s surname
with the year of publication.
References list: is a list of all the sources that you have cited within
your work
Do not include degrees like MB, Phd,- Do not use et al., but write all the
author’s names
Journal articles
Author surname, initials. (Year) Title of article, Journal Title.
Volume number (issue or part number), pp. first and last page numbers.
Gilbert, D. A and Hayes, E. (2009) Communication and outcomes of
visits between older patients and nurse practitioners. Nursing Research.
58(4), pp. 283-293
Conference papers
Author surname, initials. (Year) Title of conference paper. In:
conference proceedings title, including date. Place of publication:
Publisher.
Webb, N. L. (2004) Mathematics education reform in California. In:
Science and mathematics education in the United States: eight
innovations: proceedings of a conference, Paris, 2000. Paris: OECD.
Thesis of dissertation
citation method; Jones, J.B. (1974) The piano and chamber works of
Newspapers
Journalist surname, initials. (Year) Title of news item. Name of
newspaper. Day, Month, pp, first and last page numbers.
Note: that ‘in’ is used to link the chapter to the book and the use of
page numbers. The year of publication is only given once.
Book volume
Author/editor surname, initials. (Years) Title. Edition. Volume Place
of publication: Publisher.
Bowling, A. (2009) Research methods in health: investigating health
and health services. Volume. Maidenhead: Open University Press.
E-book
Author/editor surname, initials. (Years) Title [online]. Place of
publication: Publisher. Available from: URL. [Accessed date].