LiFlus GX

Autoclavable Fermentor

OPERATING MANUAL
 1. INTRODUCTION

1. INTRODUCTION
This manual contains a description of the equipments, installation, operation and maintenance instruction for
the LiFlus GX system manufactured by the BIOTRON, INC

2. DESCRIPTION OF EQUIPMENT
The new developed bioreactor is well designed to provide a stable, reliable bio environment for various cell
culture applications. This system can be employed for batch culture fed-batch culture or continuous culture with
microprocessor control and PC control of pH, DO, agitation, temperature, antifoam, pump feed, vessel level
accurately.

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 1. INTRODUCTION

3. DESCRIPTION OF VESSEL
The vessels are normally decided according to the working volumes of real media and provide interchangeable
vessel and easy to scale up with various different sizes of vessels from 2.5L, 5L, 7L and 10L. The glass vessel
connected with stainless steel head plate is assembled by simple clamp. It is a convenient device to assemble
into the vessel or to disassemble from one.
There are optional choices between the single wall vessel and double (jacket) vessel.

Single wall vessel Double (jacket) vessel

The stainless steel head plate is composed of 10 location free ports.

Ports are provided in the head plate for the fixed temperature sensor,
Spager, sampling, pH electrodes, DO electrodes, a foam probe, exhaust condenser. 4 different tubes of
peristaltic pump would be connected to only one line, which could be space saving in the head plate.

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1. INTRODUCTION

<PARTS AND
STRUCTURE>

N1 Inoculum Port V1 Heater Plate

N2 Sampling Port V2 Bottom Plate

N3 Sparger Port V3 Packing

N4 DO Sensor Port V4 Support

N5 Thermowell Port V5 Glass Vessel

N6 ColdFinger Port-1 V6 Middle Plate

N7 4P Feeding V7 Top Plate

N8 ColdFinger Port-2 V8 Handle

N9 Condenser V9 DC Motor

N10 PH Sensor Port V10 Bearing Housing

N11 Transfer Line V11 Shaft

N12 Foam Sensor Port V12 Foam Breaker

V13 6th Turbine Impeller

V14 U-type ColdFinger

V15 Hexagon Nut

V16 Top Plate Bolt

V17 Wrench Bolt

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 1. INTRODUCTION

Single vesel
Double vesel Bowl vessel

4. AGITATION SYSTEM
A removable agitation DC motor located on the top of bearing housing is connected to the agitation shaft. DC
motor should be removed before the sterilization and then placed in the bearing housing after the sterilization.
The motor will provide agitation speed rage of 50 to 1200 rpm.
In case of the motor mode, it is controlled by the speed that users set and in case of the DO model, it is
controlled by the measured DO value through the DO sensor to keep the DO value that users set.

5. DO CONTROL
DO value is controlled in the range of 0 - 100%. DO value is sensed by Mettler Toledo (Ingold) electrode and
also is controlled and kept by the agitation speed. In case DO value can not be maintained by the agitation
speed, DO should be increased by the pure oxygen supplies by the use of high-pressure oxygen or oxygen
cylinder.

6. pH CONTROL
pH is controlled in the range of 2.00-12.00. The pH is measured by a glass electrode ( Mettler Toledo
electrode). Control is maintained by Aci and Base peristaltic pump by PID

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 1. INTRODUCTION

7. TEMPERATURE CONTROL
Temperature automatically controls itself in the range of 8-80. This Fermentor is able to control the temperature
by the use of circular water-bath and by the heat plate and cooling water of the bottom part in the single vessel
and also in case of bigger vessel than the volume of 10L, cold finger is used for it.
As the water for controlling the temperature does not pass the body case, it prevent the aging and corrosion of
the controlling plate and also in case f the single vessel, it is possible to control the temperature by the air
cooling. RTD (Resisteance Temperature Detector) is used for temperature measure.

8. FOAM CONTROL
Firstly, foam is removed through the foam breaking impeller and then in case of the remaining foam, it is sensed
by foam/level sensor and anti-foam is injected and removes the foam by antifoam pump.

9. AERATION
Air and oxygen can be supplied to the medium through the ring sparger. The flow rate is controlled through the
control valve, which is located on a flow meter. In case quantities of DO that users want to get by the agitation
is not provided, DO should be increased by the pure oxygen supplies by the use of high-pressure oxygen or
oxygen cylinder.
9.1. AIR SUPPLYING CONTROL
The air flowing amount is controlled in the range of 0-23L/min by the control of a flow meter and the flow meter
suitable for the using vessel is installed. If it requires more air flowing amount, the flow meter should be
replaced.

10. EXHAUST SYSTEM

Gas moisture going out to the outside is condensed and then comes back to the vessel. As the gas goes out
through the 0.2 air filter, there is no contamination without the air flowing.

11. SAMPLING SYSTEM

Sample is collected through the installed sampling line before the fermentation for the sampling of components
during the fermentation. You can use a convenient one of 2 methods following below.
- Close the gas out line, open the sampling line and then do the Sampling by the pressure.
- Connect the silicon tube linked to the sampling line to the peristaltic pump and then do the Sampling by the
pump working.

12. FED-BATCH SYSTEM

Add a small quantity of the substrate continuously after measuring DO by the added substrate of low
concentration in the early process of fermentation as the indirect post. There are four type of Fed Batch mode
in this system. User can select one of the mode to feed the medium or a special substrate to the fermenter.

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 2. INSTALLATION PROCEDURE

1. INSTALLATION POSITION
1. Locate this unit where the air circulation is easy.
2. Install this unit on the place, which has no incline and is stable.
3. Use 220V main power.
4. Install this unit on the place, which is able to supply the cooling water or temperature controlling
water, or be ready the cooling-water bath.
5. Install the unit on the place providing stable temperature & moisture level.

2. FERMENTOR INSTALLATION
1. Vessel:
① Install baffle inside of a vessel.
② Mount the foam breaker onto the shaft of suitable place for the using things. (Fix it a little bit
over the water surface for removing the foam)
③ Install impeller with even distances on the shaft below the foam breaker.
④ Install various sensors (Temperature, pH, DO, Foam sensors) with sealing rubber inspect
whether it is suitable for the orifice on the surface of top plate.
⑤ Connect condenser with rubber holder and connect silicon tube the end of condenser.
⑥ Use vessel clamp to seal between the top plate of vessel and flange.(★ use screwing bolt to
tighten the clamp very slowly and firmly.)

2. Heating plate
① Locate on the place, which is not too far from the main control case
② Heating line (3 pin) and cooling line (6 pin) connect between port of main case and heating
port.

3. Main control case:

① Connect the main power supplying line (220V).
② Connect each motor cable (6 pin), temperature cable (3 pin) and foam sensor cable (2 pin) to
the ports of main case.
③ Install water-supplying line for cooling water and temperature controls.
★ Refers to water supplying line Be careful of safety accidents.
④ Connect the rube of the air pump to the Air in of the main case.
⑤ Check the airflow by operating Air pump and check airflow meter.

4. Motor installation:
① Check the connection of coupling between the inside bottom cap of motor and topside of
housing of vessel top plate.
② If the motor is installed on the housing of vessel top plate, there is 5mm of differences.
(Refer to the picture following below)
③ If you turn the motor, the differences are disappeared.
④ Tighten the nut located in the bottom side of motor to make sure that motor does not move.

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 2. INSTALLATION PROCEDURE

②→③

Coupling
Coupling disk herb
Coupling
5mm

5. Reagent bottle
Put each reagent (acid, base, anti-foam, other supply solution) into each reagent bottle.
Amongst the pipes (2 units) connected to the reagent bottle cap, one is connected to the air tube
by the silicon tube and the other one is connected to the both parts (the length for the outside
of the cap to the inside of the cap for sinking to the reagent) with the silicon tube. Insert the
both-direct fitting into the end of the silicon tube to make it easy to connect with the connecting
tube of the vessel peristaltic pump after the sterilization.
Sterilize on condition that the cap is not closed too tightly but a little bit opened.
Close the silicon tube connecting the air filter by the use of clamp for a tube before the
sterilization and then wrap all the air filter with the aluminum foil not to get wet the inside of the
filter during the sterilization.
Close the silicon tube that both-directs fitting is connected with clamp for a tube and then wrap
the end part with the aluminum foil.

6. Connection between peristaltic pump and tube

① Open transparent acrylic cover.
② Insert a tube to the bottom part of the 2 white nippers fixing the tube. (The end of the tube
outside the peristaltic pump is connected with the reagent bottle tube.)
③ Attach the tube to the inside of the surface of pump and turn on the pump switch.
④ The tube is held and fixed when the pump is turning.
⑤ Fix the held tube to the white nipper (the to part).

Before After

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 3. STERILIZATION METHOD

1. STERILIZATION
1. Clean each part of vessel.
2. After extending the baffle, then install it not to follow the shaft turning.
3. Install impeller on the shaft with consideration of the using volume.
4. Install the parts on the top plate of vessel with sealing caution.
5. Change the installation according to the users’ needs. (pH sensor, DO sensor, foam sensor,
sampling line, peristaltic pump connecting line, sparger, air filter and condenser…)
6. In case of the foam sensor, use the port cap (has a small hole on the top of the cap) possible to
insert the folder.
7. In case you use the pH sensor, install it after the calibration.
8. After the calibration, keep the power of controller turning on until you finish to use it. Sterilize
by covering the electrode of probe with a cap not to get wet.
9. In case of using the DO sensor, do the calibration after the sterilization.
10. Sterilize by covering the electrode of probe with a cap not to get wet.
11. Install the clamp to seal the vessel and top plate.
12. Be careful of the glass part of vessel not to install by force.
13. Connect by using the silicon tube for the part (sparger, reagent bottle, condenser) that needs
the air filter and also close perfectly the front and back of the air filter with clamp for a tube.
14. In case of using the peristaltic pump, connect the acid, base, anti-foam and peristaltic pump 3
ports (4 ports) with the silicon tube and close each tube with clamp for a tube.
15. Sterilize only each bottle and then it can be used by the connection with each vessel. But after
sterilizing the end of each valve, fix the connector in it for the convenient connection and then
close it with aluminum foil.
16. When the condenser is connected, disassemble the fitting itself that silicon tube is connected
to and sterilize.
17. If there is nothing connected to the top plate, close the rest port with rubber cap for sealing.
18. In case of the double jacket type, filling half of the jacket with the distilled water is good for
the heat transmission but actually it does not matter that the distilled water is perfectly
eliminated for easy transmission.
19. After the sterilization, in case of closing the opened port and separately sterilizing the medium
after getting cold, add the medium within the bench. When the inoculation inside bench, it does
not need much of caution but when the inoculation in the outside, be careful not to be
contaminated by sterilization. Connect the air line and cooling-water line.
20. Connect the pH, DO, antifoam sensor with the cable.
21. In case of the foam sensor, the one for a folder of the sensor cables should be accurately fixed
in the hole of the port cap.
22. Set the temperature, agitation and aeration.

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 3. STERILIZATION METHOD

* In the situation that the distilled water fills it, open the top out line not to destroy the vessel by
tension.
Do the sterilization
The condition of sterilization is operated at the time of 121, 20 minute. It can be sterilized for a long
time by depending on the users.
When the sterilization is working, open one of the ports no to destroy the vessel by tension.
When the sterilization is working, it depends on the users’ habits to sterilize the medium in the
vessel together. In case of sterilizing after separating the medium from the vessel by a specific
factor, sterilize the vessel, which is filled with the vessel factors and water except the specific
factors and sensor is closed.

2. OPERATING STATUS IN THE CALIBRATION

- It can be calibrated right after the main power on.
- In case the operating status of control panel is ‘RUN’, calibration does not work so after the
calibration of the stop key, press the ‘RUN’ and then it operates.

3. TEMPERATURE CALIBRATION METHOD

1.Turn off the main power switch when the unit stops. Turn on the main power switch, pressing the
temperature setting key. In case the temperature value is different from the real standard
temperature value, input the temperature value of standard thermometer by the up-down control
key (do not press the enter key) and then turn off and on again the power switch. Check the
temperature value on the panel and value of the standard thermometer.

4. pH METER CALIBRATION METHOD

1. Connect pH sensor to cable.
2. Prepare pH buffer solution (7.0, 4.0, 10.0).
3. Stop to operate vessel. (Stop stage)
4. Press pH calibration key.
5. Signal of calibration pH 7 is lightened and also pH in the agitation window and cal signal in the
pH window flash.
6. Put pH and temperature sensors into 7.0 pH buffer solution and wait until the value is stable.
7. During the calibration of pH 7, the measure value of pH 7 buffer solution shows up on the pH
window.
8. If the pH 7 is calibrated, pH 7 is flickering on the pH window and automatically the lamp of
calibration pH 4 is turned on.
9. If the lamp of 4.0 turns automatically on, put the pH sensor and temperature sensor into the
buffer solution 4.0 and wait until the value is stable.
10. During the calibration of pH 4, the measure value of pH 4 buffer solution shows up on the pH
window
11. If the pH 4 is calibrated, pH 4 flickers on the pH window and automatically the lamp of
calibration pH 10 turns on.
12. If the lamp of 10.0 turns automatically on, put the pH sensor and temperature sensor into the
buffer solution 10.0 and wait until the value is stable.
13. If the calibration of the pH 10 is finished, the indicating lamp is turned off, the ‘END’ flickers
on the pH window and then pH calibration is finished.
14. Until the fermentation is finished, keep the controller of the fermentor main case turning on.

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 3. STERILIZATION METHOD

☞ On condition of showing the stable values of each pH 4, 7, 10 buffer, if the time converting into the
next step takes long, user can press the calibration set key to keep going to the next step.
Keep your pH electrode with electrode solution or other solutions to prevent the electrode from drying
(solution is provided by Mettler Toledo).

5. DO METER CALIBRATION
1. Connect DO sensor to cable.
2. Stop the operation of vessel. (Stop status)
3. Set the 500rpm (inputting the motor speed value depending on users) of motor speed. (During
the calibration, the set value of motor speed can be changed at any time)
4. Press the DO Calibration key.
5. 0% of calibration DO lamp turns on, the operating motor speed value shows up on the agitation
window and also the dcal indication flickers on the pH window.
6. During the calibration of 0%, the ‘0’ value flickers on the DO window.
7. If the 0% is automatically calibrated, the 0% lamp turns and also automatically 100% lamp is
turned on.
8. During the calibration of 100%, relative DO value, which is read as DO sensor, shows up on the
DO window.
9. If the aeration and agitation are supplied most highly, 100% calibration is possible by pressing
the set key once more.
10. If the calibration of 100% is finished, ‘END’ indication flickers on the pH window and the DO
calibration is finished.
This Fermentor fixes ‘0’ degree of saltiness inside.

6. FOAM CONTROL METHOD

Turn the foam tunings controller on the left side of panel to the opposite direction of clockwise
rotation and the sensitivity of water and foam will increase when it turns to the clockwise rotation.
With the exception of a special case, as the antifoam does not dwindle, it does not have to be
continuously put into.
A small quantity of foam is removed through the foam breaker of a physical method.

Caution: As the sensitivity reacting according to the type of foam and depth of sensor touching needs
to be controlled, the control for the sensitivity key also needs. If the foam tunning key turns to the
clockwise rotation, the sensitivity increases.

7.ADDING NUTRIENT METHOD

Supplying nutrient method is following below.
- Automatic feeding method by the DO value (optional specification of peristaltic pump for feed)
- Automatic feeding method by the pH value (use of pH peristaltic pump)
Feeding by computer control. (It requires additional devices.)

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 4. Control System Operation – 1. Introduction

1.1 Function introduction

1.2 The touch screen

This system is developed touch screen for display all of data and for the operation. The user can
select the function menus and setup parameters by touching the corresponding keys on the screen.

1.3 Window composition

All of the windows are composed of the title bar, working area and the function keys. The name of
window is displayed in the title bar. In working area all of the operation data can be displayed and
the operator can select and set parameters.

1.4 Numerical input

You can use the numerical input Key Pad to setup parameters.

Clicking it, number of the Set Value is deleted

from right to left one by one.

After Clicking it, Message Box is disappeared

and it comes back to SET Menu.

After Set Value input, Clicking Enter key,

Message Box is disappeared and it comes back
to SET Menu. You can confirm the value changed
in Set Menu screen.

▲ Numerical input

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 4. Control System Operation – 2. Main

Turn on the main power, the main screen below is displayed.

◀ Main Screen

Title Bar:
BioTron: The company name, click it you can find the product information.
Main: The name of current screen.
Date & Time: Display the current time, click it you can set the date and time.
Working Area:
Mode: The name of main parameters of fermentation, click it you can enter to the calibration process.
S/V: Displays the present set value.
P/V: Displays the present measured value and operating value.
Foam sensitive: It indicate the foam sensor sensitivity, the bigger the number, the more sensitive the
sensor.
Start time: Click it, system starts to record the fermentation time.
Cumulative time: Click it, system stops recording fermentation time.
Menu Bar:
Calibration: Select this key, it moves to the Calibration Menu screen.
Setup: Select this key, it moves to the Setup Menu screen.
Control: Select this key, it moves to the Control Menu screen.
Data Log: Select this key, it moves to the Data Log Menu screen.
System: Select this key, it moves to the System screen.
Run and Stop:
Run/Stop: [Run] shows the status that fermenter system is stopped and [Stop] shows the status that
fermenter system is in operation.

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 4. Control System Operation – 2. Main

2.1 Product information

Touch the [BioTron] in the Main Screen, the Product Information
box as below will pop up and show
the product name, version of the current program, the company
name and website which developed this system.

product information ▶

2.2 Date and Time

Touch the [Date & Time] in the Main Screen,
the Date & Time Set window as below will
pop up. 현재시간이 표시된다.
User can set the date and time by input number
using Numerical Input Key Pad.
년도 월 일

시간 분 초

Date & Time Set ▶

2.3 Set Value

Touch the [S/V] of each parameter in the Main Screen, the Numerical Input Key Pad will pop up. The
user can input the set value use the Key Pad.
To select the [Foam sensitive] button user can setup the sensitivity of the foam sensor.

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 4. Control System Operation – 2. Main

2.4 Calibration
Select the [mode] of each parameter, then enter to the corresponding parameter’s calibration screen.
In this system you can calibrate pH sensor, DO sensor, Temperature sensor and four peristaltic
pumps by this way. While the pH or DO is selected, you can also look over the recent calibration
information on the Cal Status screen. Here the DO is shown as one example.

Click the [DO]key in the Main Screen, then the above selection window will pop up.
If the [DO calibration] selected, the DO Calibration Screen will be displayed and you can calibrate
the DO sensor (refer to 3.2 DO Calibration).
If the [Cal status] selected, the DO Cal Status below will pop up.

▲ DO calibration Menu ▲ DO calibration status

2.5 Fermentation time

If the fermentation started, you must click the [Start time] on the Main Screen. Then the system
record fermentation time automatically, and the time shows in the [End time] button. When the
fermentation is finished, you can click the [End time] manually to stop the time record.

2.6 Trend Graph

Click any place of working area, the trend graph window pops up to show the real time recorded data.

2.7 Run
Click the [Run] on the Main Screen, the fermenter system is operated. During the fermenter running,
the [Run] key changed to [Stop]. Click this key, the fermenter is stopped.

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 4. Control System Operation – 3. Calibration

Click the [Calibration] on the Main Menu of the Main Screen, the below selection window pops up
and you can calibrate the sensors and pumps etc.

Select one of the items, then you can enter the calibration
process of the parameter.

Calibration Menu ▶
3.1 pH Calibration
Get into the pH Calibration process, there are three
options, such as the normal pH Calibration,
pH ReCalibration and Cal Status. The normal calibration
can be done using two or above pH buffers before
the fermenter sterilization. In this fermenter system,
user can choose the discretional two or three
buffers between pH7, pH4, pH10 buffer to do
calibration. The pH ReCalibration is done during
fermentation to correct the sensor if any uncertainty
measurement observed.

3.1.1 The normal pH Calibration ▲ pH Calibration Menu

Click the [pH Calibration] on the pH Calibration option
window to enter the above screen. Then you can do the
pH calibration following the below procedure.
• Put the pH sensor into the discretional pH buffer (eg. pH7),
then the present measured values display in the pH, Temp,
mV cells at the top left corner of the screen. To start
calibration, click the corresponding beaker image key
(eg. [pH7]).
• The calibration is done automatically and the progress
bar indicates how far the calibration has progressed.
• After confirming that currently measured value of buffer
is stable, click the [Nex cal] to finish calibration
manually.
• Pull out the sensor from the buffer and cleanse sensor ▲ pH Calibration
glass part with clean water (sprayer).Get rid of moisture
with dry soft paper or cloth.
• Put pH sensor into another buffer solution. The operating method is same as above.
• If the calibration finished, click the [OK] to exit.
• Click [Cancel] to cancel it during buffer calibration and exit the pH Calibration screen.

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 4. Control System Operation – 3. Calibration

3.1.2 pH ReCalibration
Click the [pH ReCalibration] on the pH Calibration option
window to enter the above screen.
Then you can do the pH Recalibration following
the below procedure.
• The present measured value displays in the <process value>.
• Take out some ferment medium from the fermenter.
• Measure the pH value of the medium sample using other
standard pH measure device.
• Input the measured value from the exterior device into
the <ReCal Set Value> cell. Then click [Cal Start] to start
calibration.
• If the calibrating finished, the input value shows in the
<process value>cell. Then click [close] to exit the pH
Recalibration procedure.
▲pH ReCalibration

3.1.3 pH Calibration Status

Click the [Cal Status] on the pH Calibration option window to enter the
above screen. You can look over the recent pH calibration information
from it.

pH Calibration Status ▶

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 4. Control System Operation – 3. Calibration

3.2 DO Calibration
Click [DO Calibration] on the Calibration Menu screen to enter the DO Calibration window as above.
Then you can do the DO calibration following the below procedure.
• Before start the DO calibration you must open the air
supply, and set the fermentation temperature and the
agitation speed in the Setup screen.
The setup method will discuss in Chapter 4.
• To start Zero calibration, Click the beaker image key
[Zero]. In this system the zero calibration do
automatically.
• If the present measured value in the top left corner
shows 0%, that indicates the zero calibration finished.
• Click the beaker image key [Sat], to start span
calibration. Then the agitation speed and the
temperature reach to fermentation value.
• If the present measured value in the top left corner
shows 100%, that indicates the span calibration finished.
• If the calibration finished, click the [OK] to exit.
• Click [Cancel] to cancel it during buffer calibration
and exit the pH Calibration screen.
▲DO Calibration

3.3 Temperature Calibration

Click [Temperature Calibration] on the Calibration Menu screen to enter the Temperature
Calibration window as above. Then you can do the Temperature calibration following the below
procedure.
• The measured temperature value displays in the
<P/V> cell.
• Put other standard temperature sensor to the same
solution(or also set the two sensor in air), get a
temperature value.
• Click the [Set temperature input], and input the
measured temperature value from the exterior sensor.
That value displays in the <P/V> cell.
• Click the [OK] to finish the temperature calibration
and exit.
• Click [Cancel] to cancel calibration and exit the
Temperature Calibration screen.

▲Temperature Calibration

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 4. Control System Operation – 3. Calibration

3.4 Pump Calibration Menu

Click [Pump Calibration] on the Calibration Menu screen to enter
the Pump Calibration window as
above. Then you should select one of the four pumps. But the
calibration procedures of four pumps are the same.
And there are two options in pump’s type.
One is the constant speed pump, and another is the variable
speed pump.

▲ Pump Calibration Menu

If the pump is constant speed type, the calibration screen is as below.
The calibration procedure is:
• Place one end of the tubing into a beaker filled with water
and the other end into a measuring beaker.
Complete fill the tubing with water. For this you can
manually activate the pump.
• Input the active time to the <active time input>.
• Start the calibration of the pump via [pump on start].
• The pump will automatically stop after the inputted time
passed.
• Measure the volume of liquid transferred, and input this
value to the <Volume>.
▲ Constant speed pump calibration
• The system automatically calculates the flowrate
considering the inputted active time and volume and displays the result in the screen.
• Click the [OK] to finish calibration and exit the window.
• Click [Cancel] to cancel calibration and exit the Pump Calibration screen.

If the pump is variable speed type, the calibration screen is as below.

◀ Variable speed pump calibration

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 4. Control System Operation – 3. Calibration

3.5 Gas calibration

(It is an optional function.)

3.6 Pressure calibration

(It is an optional function.)

◀ Pressure Calibration

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 4. Control System Operation – 4. Setup

Click the [Setup] on the Main Menu of the Main

Screen, the below Setup Menu window pops up and
you can do setup for various parameters.

Setup menu ▶

4.1 pH Setup
The pH of fermentation is controlled using the acid and base pump.

Click [pH Setup] on the Setup Menu screen to enter the

pH Setup window as above. Click each of the cells to
set the pH Set Value, acid and base pump’s on/off time.

pH Setup ▶

4.2 DO Setup
If you choose the [DO Setup] on the Setup Menu screen, it moves to the DO Setup window.
Then you can input the DO set value by Numerical Input Key Pad.

4.3 Temperature Setup

If you choose the [Temperature Setup] on the Setup Menu screen, it moves to the Temperature
Setup window. Then you can input the temperature set value by Numerical Input Key Pad.

4.4 Agitation speed setup

If you choose the [Agitation Setup] on the Setup Menu screen, it moves to the Agitation Setup
window. Then you can input the agitation set value by Numerical Input Key Pad.

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 4. Control System Operation – 4. Setup

4.5 Foam setup

Click [Foam Setup] on the Setup Menu screen to enter the
Foam Setup window as above. Click each of the cells to set
the foam Set Value, foam pump’s on and off time.

Foam Setup ▶
4.6 Feed pump setup
Click [Feed Setup] on the Setup Menu screen to enter the
Feed Setup window as above. Click each of the cells to set
the feed pump’s on and off time.

Feed Setup ▶
4.7 Pressure setup
If you choose the [Pressure Setup] on the Setup Menu screen, it moves to the Pressure Setup
window. Then you can input the pressure set value by Numerical Input Key Pad.

4.8 Alarm Setup

Click [Alarm Setup] on the Setup Menu screen to
enter the Alarm Setup window as above.
In this system you can set the alarms for 9
parameters, pH, DO, agitation speed, acid pump,
base pump, foam pump, feed pump and pressure.
These parameters can be selected optionally and
the condition, such as low and high level, should
be inputted by the operator.

Alarm Setup ▶

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 4. Control System Operation – 5. Control Mode

Click the [Control] on the Main Menu of the Main Screen, the below Control Menu window pops up.

Select one of the buttons to enter each control mode setup

process. To exit the window, click the [close].

Control Menu ▶

5.1 Fed Batch Setup

Click [Fed Batch Setup] on the Control Menu screen to
enter the Fed Batch Setup window as above.
There are four options in the Fed Batch Setup screen,
pH stat, DO stat, Continuous culture, User Feeding,
Glucose and Not use. Select one of these modes, and
click the [setup] to setup that mode.

FedBatch Setup ▶
5.1.1 pH stat
Select [pH stat] on the Fed Batch Setup screen and
click the [setup] to enter to the below window.

Description of Fed Batch Setup-pH stat screen:

- Set Point: pH set value
- Active Level: If the [HI] selected, the feed pump
runs when the current measured pH value is higher
than the Set Point; and if the [LO] selected, the feed
pump runs when the current measured pH value is
less than the Set Point.
- Delay Time: The time for the feed pump switch
over.
- Feed Pump: For set the pump’s on and off time.
- pH Control Use: Control the pH of the fermentation
by acid and base pump while do the fed-batch culture. ▲ FedBatch Setup pH Stat

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 4. Control System Operation – 5. Control Mode

5.1.2 DO stat
Select [DO stat] on the Fed Batch Setup screen and click the [setup] to enter to the below window.

Description of Fed Batch Setup-DO stat screen:

- Set Point: DO set value
- Active Level: If the [HI] selected, the feed pump
runs when the current measured DO value is higher
than the Set Point; and if the [LO] selected, the
feed pump runs when the current measured DO value
is less than the Set Point.
- Delay Time: The time for the feed pump switch
over.
- Feed Pump: For set the pump’s on and off time.
- DO Control Use: Control the DO of the
fermentation by acid and base pump while doing the
fed-batch culture.

▲ FedBatch Setup DO Stat

5.1.3 User feeding
Select [User feeding] on the Fed Batch Setup screen and click the [setup] to enter to the below
window.

Screen description:
- [Current Step(state)]: It displays the number of
current step that is in progress.
- [STEP]: It is the number of feeding sequence.
- [TIME(min)]: It is the feeding time, every step’s time
can be set differently.
- [S/V(ml)]: It is the total volume of each step.
- [Start]: Click this button, start the feeding step from
STEP 1.
- [Pause]: Click this button during the steps progress,
stop the progress.
- [Stop]: Click this button, stop the step progress, and ▲ User Feeding Pump Control
you can restart from STEP 1.
- [Close]: If the setup is finished, click this button to exit this screen.

Operation: 　
• Input the each step’s time and total volume.
• Click the [Start] button to start user feeding pump control mode.
• The system automatically implements these steps according to the step’s sequence number.
• Click the [Pause] button during the steps progress, stop the progress. And to continue the step,
you can click the [Start] button.
• Click [Stop] button, stop the step progress, and you can restart from STEP 1.

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 4. Control System Operation – 5. Control Mode

5.1.4 Continuous culture

If the [Continuous culture] on the Fed Batch Setup screen is selected, the feed pump will
continuously feeds the substrate or medium according to the on/off time which set in the Feed Setup
screen (refer to the 4.6 Feed Pump Setup).

5.1.5 Glucose
(It is an optional function.)

5.1.6 Not use

If the [Not use] in the Fed Batch Setup screen is selected, the feed pump not automatically feeds the
substrate or medium.

5.2 DO Control Mode

Click [DO Control Setup] on the Control Menu
screen to enter the DO Control Mode window as
above. There are four cascade control mode in
the DO Control Mode screen, RPM Control,
Gas Control, Air Flow Control and the Press
Control modes. You can click the [Setup] key to
setup each control mode and in the right part of
the above screen can set the control sequence.

5.2.1 DO Control mode Setup ▲ DO Control Mode(Cascade Select)

5.2.1.1 RPM Setup
Screen description:
- [Action Delay Time]: Delay time for the motor
switched over.
- [Maximum RPM]: The maximum value that the
agitation speed can reach.
- [Increase Step]: Steps in which the agitation speed
increases.
- [Increase Time]: The time of speed increasing.
- [Stable RPM Return]: If the RPM increasing can
not fulfill dissolved oxygen requirement, then the
RPM return to the Set Value and the Gas mode is
switched. ▲ DO Control-RPM Setup
- [RPM After Gas Insert]: If the RPM increasing
can not fulfill dissolved oxygen requirement, then the RPM maintain the maximum value and the Gas
mode is switched.
Operation: 　
• Set the each parameter using Numerical Input Key Pad.
• Click the [Close] button, the setup box disappeared and it returns to the DO Control Mode window.

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 4. Control System Operation – 5. Control Mode

5.2.1.2 Gas Setup

If the [Gas control] on the DO Control Mode screen is selected
and click the [Setup] key, the above screen pops up.
You can select the kind of gas and input the valve’s on/off time
for control the dissolved oxygen level.

5.2.1.3 Air flow Setup

(It is an optional function.)

5.2.1.4 Pressure Setup

(It is an optional function.)

▲ DO Control-Gas Setup
5.2.2 Control Sequence Select
If you click the [Control Sequence] from 1 to 4, then
the above selection box pops up. You can choose one of
them, then click the [Close] key to return to the
DO Control Mode screen.

▲ Control Sequence Select

5.3 Interface Setup
Click [Interface Setup] on the Control Menu screen to enter
the Interface Menu window as above. There are 9 items in the
Interface Menu screen, pH, DO, Temp, Level, Balance, Turbidity,
YSI2700 Glucose Analyzer, Gas Mixer, Aux3.

▲ Interface Menu

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 4. Control System Operation – 6. Data Acquisition and Storage

Click the [Data Log] on the Main Menu of the Main Screen, the
below Data Log Menu window pops up.

DataLog Menu ▶

6.1 Data Log Setup

Click the [Data Log Setup] on the Data
Log Menu window, the Data Log Setup
window pops up as below. User can
select the kind of data logging devices
and the data items. The data log interval
time and the user name can be setup.

DataLog Setup ▶

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 4. Control System Operation – 7. System

Click [System] button on the menu to advance to

[Lock Warning] window where you put ID and PASSWORD.
As default ID : BIOTRON / PASS : 1945
※ Caution - System menu involves the setting and operation
of the whole system in GX, it is prohibited for user to enter into
the menu.

Description:
-. Click [Diagnostic] to move on to the module test window.
-. Click [System Configuration] to move on to system configuration window
-. Click [Advanced User Configuration] to move on to the advanced user
configuration window
-. Click [Close] to exit and return to the main window ▲ System

7.1 Diagnostics Menu

Touch [Diagnostic] button on the <System> to go to the [Diagnostics Menu]

Description:
-. Click [Protocol Test] to screen protocol status of each data module and report.
-. Click [Temp] to display first setup data in graph and temp related values,P,I,D,Heater and Cooler, are able to
be set-up.
-. Click [Variable/Constant] to configure time interval of output data.
-. Click [H/W Port test] to test hardware of Pump,
Protocol and means of protocol.
-. Click [Motor Test] to examine the user configured
inverting rate and frequency.
-. Click [Flash Test] to examine Flash memory.
-. Click [Close] to exit to the main window.

▲ Diagnostics Menu

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 4. Control System Operation – 7. System

7.1.1 Protocol Test

Touch [Protocol Test] on the <Diagnostic Menu> screen to go advance to the screen for testing state of protocol
between CPU and Sensor Board.

Description:
[GX/Double/Multi/PC]: select one among the radio button. Here you choose the GX.
-. Click [send 1 packit/receive 1 packit] to send and receive packit in 1 unit.
-. Click [ACK Code/ASCK Code] to receive a text message of ACK/NACK Code after sending packit.
-. Click [Auto send/receive] to make the protocol among modules correspond automatically.
The result data from Sensor B/D is shown in mV units and if you click this button again, it makes the auto
send/receive stop. When you can see variable mV value, nothing is failure, this function is for checking error in
sensing.
-. Click [Remote On] to help correspond data wirelessly.
-. Click [ID] to change protocol channel for multi fermenter.
-. Click [Close] to exit this window.

▲ Protocol Test Screen

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 4. Control System Operation – 7. System

7.1.2 Temp Test

Click [Temp Test] button on the <Diagnostic Menu> screen to go advance to the window as below. Input
Proportional(P), Integration(I) and Derivative(D) constant applied optical constant. You might not need to
change these digit value later. This screen shows PID constants as graph.

Description:
-. Kp const: this is for P to increase temperature control rate.
-. Ki const: this is for I to increase temperature control rate.
-. Kd const: this is for D to stabilize temperature into safety range.
-. As per above constants, you can confirm P,I,D and also temperature in Temp P/V cell.
-. Input temperature value onto Sampling Time cell for configuring temp.
-. Set temp value which you want to reach finally in Temp S/V cell.
-. You can see calculated digit value on Heater, Cooler cells as ON or OFF sign.
-. Click [Start] button to see starting time, cumulating time and sampling time, this button also shows you the
maximum and minimum value for confirmation and graph of test status.
-. Close pop screen.

▲ Temperature Test

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 4. Control System Operation – 7. System

7.1.3 H/W Test

Click [H/W Port Test] button on the <Diagnostic Menu> screen to go advance to the window as below. this is for
checking if CPU controls all of the functions as programmed, generally it is used for quality control under
manufacturing.

Description:
[Valve]- test all of the 16 valves.
[Pump]- test each Pump’s condition.
[Temp]-test the signal condition of Heater and Cooler.
[Motor]-check Motor’s condition. It figures out if there is any variable error.

▲ H/W Port Test

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 4. Control System Operation – 7. System

7.2 System Configuration

Consisting menus in
[System Configuration Menu] are under development.

Click [System Configuration] button on

the <System> to go advance to the window as right side.

Description:
This screen which sets system properties
is composed of menus for each sensor and
H/W system. This is optional menu and specialized
for connecting other programs for example
when we connect other manufacturer’s brand products.

System Configuration ▲

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 4. Control System Operation – 7. System

7.3 Advanced User Configuration

Touch [Advanced User Configuration] button
on the <System> screen to go advance to the window as right side.

Description:
This menu is for meeting advanced user’s needs and
it moves on to each cascade menus by selecting
on the menu screen.

7.3.1 pH Configuration

Click [pH Configuration] to set pH value from Advanced User Configuration ▲

the <Advanced User Configuration> screen.
This is pH advanced configuration window.
[Offset]- input an aberrational pH value which is out of control by setting pH.
[Calibration Automatic Range]- it calibrates pH range automatically in buffer solution then the result is saved.
[Calibration Temp Compensation]- “YES” to select temp compensation option and “NO” not to use.
[Buffer 1] ~ [Buffer 3] – input pH value of reagent to use when calibrating pH.
(ex. “4.0”, “7.0”. “11.0”)

pH Configuration ▶

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 4. Control System Operation – 7. System

7.3.2 DO Configuration
Click [DO Configuration] button on
the <Advanced User Configuration> screen
to go advance to the window as right side.

Description:
DO advanced configuration menu.
[Display Mode] – select one among
[DO], [O2], [pO2], [Air]
[Calibration Temp Compensation]-
“YES” to use temp compensation option
and “NO” not to use.
[Offset]- input DO aberration value
range from + to –
[Altitude]- input necessary digit value
for calculating in accordance with altitude of DO sensor.
[Salinity]- input necessary digit value
for calculating in accordance with level of salinity.
▲ DO Configuration

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 4. Control System Operation – 7. System

7.3.3 Temp Configuration

Click [Temp Configuration] button on
the <Advanced User Configuration> screen
to change and re-input the temp value on it.

<Description>
[Temp Control] – choose the temperature control
method
LiFlus GX-PID : control the temperature by PID
LiFlus GX-OnOff : control the temperature by the
OnOff table set by the user.

WaterBath : connects with Water Bath by RS232

Communication and control by the Water Bath.

[Proportional Const]: to configure proportional ▲ Temp Control & Sensor Configuration

constant for controlling temp, the higher constant
the more efficient in controlling temp, however
it can not reduce an aberration. Though it reduces an aberration in normal condition, it may cause the over
shoot effect for much response. (Range: 0.00~100.00)

[Derivative Const]: it shows derivative constant for temp controlling. It enhanced stability so that
it reduces over shoot effect and much response. (Range: 0.00~100.00)

[Integrate Const]: set the integral value for the temp. control.
It has the advantage of removing normal status error, but it may also cause the functional degrade by much
response. It causes the over shoot effect by much response. (Range: 0.00~100.00)

[Error] : input the Error(difference) between the temperature setting value and actual one.
(A unit: ℃, Range: 0.00~100.00)
[OnTime] : input the OnTime in one cycle at the Heating and Cooling when it is above the
relevant error value. (A unit: sec., Range: 0.00~100.00)
[OffTime] : input the OffTime in one cycle at the Heating and Cooling when it is above the
relevant error value. (A unit: sec, Range: 0.00~100.00)

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 4. Control System Operation – 7. System

7.4 Other
Click [Other] button on
the <Advanced User Configuration> screen
to move on the window as right side.

Description:
[Variable-Default]: Default all configured values.
[ID&Password] for configuring Screen Lock and
System access
Agitation Setup for configuring a type of motor
and hardware control.
User Login for unlocking Screen Lock.
Vorvertrag Work for scheduled operation.
Remote Control for PC, Double, Multi channel.

7.4.1 ID & Password ▲ Advanced User Configuration Other

Click [ID & Password] button on
the <Advanced User Configuration Other> screen
to move on the window as right side.

Description:
Input digit among 1 to 9 value as ID and Pass word,
press SAVE to save vice versa.
Click [Check ID] to avoid using
the same ID with other users.

ID & Password ▲

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 4. Control System Operation – 7. System

7.4.2 Agitation Setup

Click [Agitation Setup] button on
the <Advanced User Configuration Other> screen
to move on the window as right side.

Description:
[Maximum RPM]- input the maximum RPM
[Minimum RPM] – input the minimum RPM.
[Increase Setup]- input increasing steps
as digit value.
[Step Increase Time]- input the interval
time of increasing step.
[Motor Select] – Geared DC low speed motor
is used for controlling low RPM rate with gear
motor.
[Gear Rate]- identify gear rate.
[Start PWM]- configure initial motor frequency.
It help avoid becoming motor rate slow.

▲ Advanced User Configuration Agitation

7.4.3 User Login
Click [User Login] button on
the <Advanced User Configuration Other> screen
to move on the window as right side.

Description:
Choose Screen Lock option if
“USER” to use or “NOT USER” not to use.
7.4.4 Vorvertrag Work
Click [Vorvertrag Work] button on t
he <Advanced User Configuration Other> to go advance
▲ Screen Lock Display
to the window as right side.

Description:
It asks you whether you use reservation work,
if it is set, Vorvertrag work button is displayed
on main screen.

▲ Vorvertrag Work Display

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 4. Control System Operation – 7. System

7.4.5 Remote Control

Click [Remote Control] button on
the <Advanced User Configuration Other> screen
to move on the window as right side.

Description:
-. Click [ID NO] to input ID
-. Click [Control] to decide to use
[Remote Control] function.

▲ Remote Control Display

7.5 Analog data input/output
Click the <System> button at the bottom of main menu to go advance to the <Advanced User Configuration>
screen then touch the <Other> button to move on to the <Advanced User Configuration Other> window. There
are many consists of the menu, select <Analog Input Setup> button among others to go to the window as
below.

Window Description
-. Click [A1~A8] to initialize as hardware
of 3~4 variable pumps for AirFlow(MFC), O2 and CO2
-. Click [Analog] to input analogue’s
max and min value.
-. Click [PV Value Setup] to display
analogue hardware’s max and min value (%).
For example, electronic analogue’s
sign is input in [Analog] cell and
it results analogue data from primary equation.

Operation Description:
 .Select a hardware you want to set up
 . Click [Analogue] cell to input your max and min value.
 . Click [PV Value Setup] to input max and min value. ▲ Analog Input(Output)
 . Click [Close] to complete.

[Analogue Input] is also able to configure from touching C2 or CO2 button on the left side of the main
screen.

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