Barfoed's Test: Specific Test For Carbohydrates
Barfoed's Test: Specific Test For Carbohydrates
Barfoed's Test: Specific Test For Carbohydrates
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Barfoed’s Test
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What is Barfoed’s Test?
That is why it is specify to heat the sugar solution for not more
than 3 minutes in order to be sure that only monosaccharide will
react because they react faster than disaccharide
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How does Barfoed’s Reagent work?
Barfoed’s test uses copper Benedict’s test uses copper Fehling’s Test uses Rochelle
acetate and acetic acid sulfate, sodium carbonate and salt and copper sulfate
sodium citrate
Uses acetic acid to make an Uses sodium carbonate to Uses sodium carbonate to
acidic environment for the make an alkaline environment make an alkaline environment
reaction to occur for the reaction to occur for the reaction to occur
It is only brick-red colored The precipitate may vary in Only red precipitate is obtained
precipitate which is obtained color depending on the
concentration of sugar it may
be green, yellow, orange or red
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What test is more accurate?
Glucose
But both of the test relies in the principle of dehydration with the
use of strong acid (in the case of seliwanoff’s test is the
resorcinol in the molisch test the sulfuric acid)