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    Cdna Presentation

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    Rystiza Baro
    This document discusses complementary DNA (cDNA). It defines cDNA as a DNA copy of messenger RNA (mRNA) made using the enzyme reverse transcriptase. cDNA lacks introns and regulatory elements like promoters found in genomic DNA. It represents only expressed genes as mRNA templates. Reverse transcriptase was discovered in retroviruses and is also found in eukaryotic genomes. cDNA libraries allow study of gene expression and alternative splicing and facilitate expressing eukaryotic genes in prokaryotes.

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    Cdna Presentation

    Uploaded by

    Rystiza Baro
    32 views10 pages
    This document discusses complementary DNA (cDNA). It defines cDNA as a DNA copy of messenger RNA (mRNA) made using the enzyme reverse transcriptase. cDNA lacks introns and regulatory elements like promoters found in genomic DNA. It represents only expressed genes as mRNA templates. Reverse transcriptase was discovered in retroviruses and is also found in eukaryotic genomes. cDNA libraries allow study of gene expression and alternative splicing and facilitate expressing eukaryotic genes in prokaryotes.

    Original Description:

    The practical apllicatiinsof cDNA are explained in a simple way

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    This document discusses complementary DNA (cDNA). It defines cDNA as a DNA copy of messenger RNA (mRNA) made using the enzyme reverse transcriptase. cDNA lacks introns and regulatory elements like promoters found in genomic DNA. It represents only expressed genes as mRNA templates. Reverse transcriptase was discovered in retroviruses and is also found in eukaryotic genomes. cDNA libraries allow study of gene expression and alternative splicing and facilitate expressing eukaryotic genes in prokaryotes.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    32 views10 pages

    Cdna Presentation

    Uploaded by

    Rystiza Baro
    This document discusses complementary DNA (cDNA). It defines cDNA as a DNA copy of messenger RNA (mRNA) made using the enzyme reverse transcriptase. cDNA lacks introns and regulatory elements like promoters found in genomic DNA. It represents only expressed genes as mRNA templates. Reverse transcriptase was discovered in retroviruses and is also found in eukaryotic genomes. cDNA libraries allow study of gene expression and alternative splicing and facilitate expressing eukaryotic genes in prokaryotes.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 10

    COMPLEMENTARY

    DNA(CDNA)
    PRESENTED BY …………………………
    RUNUMI B
    REG NO.—20140
    DEPARTMENT OF MEDICAL BIOTECHNOL
    SIKKIM MANIPAL INSTITUTE OF MED
    SCIE
    DEFINITION:-
     DNA COPY OF MESSENGER RNA
     REVERSE TRANSCRIPTASE(RNA DEPENDENT DNA POLYMERASE)

     NOT THE GENOMIC DNA BECAUSE THE TRANSCRIPTS OF GENOMIC RNA HAS
    BEEN PROCESSED
     LACKS PROMOTERS AND INTRONS
     SELECTIVE EXPRESSION OF THAT PARTICULAR GENE- ABUNDANCE OF THAT
    MRANA

     EITHER IN VITRO OR NATURALLY( RETROVIRUSES)-PROVIRUS


    REVERSE TRANSCRIPTASE:-
     Discovered by Howard Temin in Rous Sarcoma Virus
    1975 Nobel Prize in Physiology or
    Medicine(Nature)
     Independently isolated by David Baltimore in 1970
    Location:-
    Eukaryotic genomes utilised by
    retrotransposons
    Telomerase
    retroviruses
    Structure:—
     HIV-1 RT(HIV TYP 1)
     M-MLV RT— 75kDa ,
    monomer
     AMV RT-
    63kDa+95kDa
     TELOMERASE RT
    Working of cDNA synthesis:-
     CDNA SYNTHESIS
    APPLICATION:
    Construction of cDNA library- study expression of
    mRNA, alternative splicing in diff. Cells or tissues
    Facilitate generation of antibodies and monoclonal
    antibodies

    Express eukaryotic genes in prokaryotes


    In REVERSE GENETICS- understand the function of a gene
    Disadvantages:-
     No introns and any other sequences that are altered after
    transcription – promoters and enhancers
     Non- expressed genes are not isolated.
     Frequency of a particular DNA Sequence in the library will
    depend on the abundance of the mRNA
     Advantage :
     Creation of a provirus by the proviruses

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