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    Crp-Dikonversi

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    Kurnia Fitriasari
    C-reactive protein (CRP) is a marker for inflammation measured in the blood. CRP levels rise in response to inflammation and an extremely elevated level suggests a possible bacterial infection. The CRP test involves mixing patient serum with latex particles coated with CRP antibodies. A positive test shows agglutination and indicates CRP is present, while a negative test shows no clumping. The test helps diagnose conditions and monitor treatment effects.

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    Crp-Dikonversi

    Uploaded by

    Kurnia Fitriasari
    271 views13 pages
    C-reactive protein (CRP) is a marker for inflammation measured in the blood. CRP levels rise in response to inflammation and an extremely elevated level suggests a possible bacterial infection. The CRP test involves mixing patient serum with latex particles coated with CRP antibodies. A positive test shows agglutination and indicates CRP is present, while a negative test shows no clumping. The test helps diagnose conditions and monitor treatment effects.

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    C-reactive protein (CRP) is a marker for inflammation measured in the blood. CRP levels rise in response to inflammation and an extremely elevated level suggests a possible bacterial infection. The CRP test involves mixing patient serum with latex particles coated with CRP antibodies. A positive test shows agglutination and indicates CRP is present, while a negative test shows no clumping. The test helps diagnose conditions and monitor treatment effects.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    271 views13 pages

    Crp-Dikonversi

    Uploaded by

    Kurnia Fitriasari
    C-reactive protein (CRP) is a marker for inflammation measured in the blood. CRP levels rise in response to inflammation and an extremely elevated level suggests a possible bacterial infection. The CRP test involves mixing patient serum with latex particles coated with CRP antibodies. A positive test shows agglutination and indicates CRP is present, while a negative test shows no clumping. The test helps diagnose conditions and monitor treatment effects.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 13

    C-reactive protein

    (CRP)
    C-reactive protein (CRP)

    is a blood test marker for inflammation in the


    body.
    CRPis in the liver and its level
    produced by testing
    measured is the blood.
    CRP is classified as an acute phase reactant,
    which means that its levels will rise in response
    to inflammation.
    • Measures of CRP add to the
    diagnostic in selected
    procedur cases
    differentiation (e-g.a in
    between the and a viral
    bacterial
    einfection).
    • An extremely elevated CRP is suggestive of a
    possible bacterial infection.
    • The CRP level may be useful also for
    monitoring the effect of treatment and for
    early detection of postoperative
    complications or intercurrent infections.
    Tests for CRP

    • Rapid latex agglutination test


    • Principle:
    – The test is based on the reaction between
    patient serum containing CRP as the
    antigen & theantibody coated
    corresponding to treate
    the surface of latex particle.The d
    enhance
    coated the detection of an agglutinate reaction
    particle
    when antigen is present in serum
    s
    the tested. being
    Tests for CRP

    • Specimen- Serum Quality control


    Include positive & negative
    control serum.
    • Reagent & materials
    required
    – CRP latex reagent
    – Glycine – saline buffer
    – Capillary pipette
    – Applicator sticks
    – Glass slide
    – Serologic pipettes & rubber
    bulb
    PROCEDURE
    A.Qualitative
    Test:
    • Bring and specimens to
    reagents
     temperature before use. room
     Place one drop (40 μl) of CRP Positive Control on field #1 of the
    reaction slide. Place one drop (40 μl) of the CRP Negative Control
    on field #2.

    • Using a serological pipette place (40μl)


    of undiluted test sample to field #3.
    PROCEDURE
    A.Qualitative Test:
    •Gently resuspend the CRP LatexReagent
    and add one drop to each test field.
    • Mix well with the provided stirring sticks.
    •Rotate the slide for 2 minutes rea
    and immediately under an oblique indirect d
    light.
    INTERPRETATION

    A.Qualitative Test:
    • A negative reaction is indicated by a uniform
    milky suspension with no agglutination as
    observed with the CRP Negative Control.
    • A positive reaction is indicated by any
    observable agglutination in the reaction
    mixture. The specimen reaction should be
    compared to the CRP Negative Control
    INTERFERENCES

    NON INTERFERING
    SUBSTANCES:
    • Hemoglobin (10g/dl)
    • Bilirubin(20mg/dl)
    • Lipemia(10g/dl)
    NOTE

    • High CRP concentration samples may give


    negative results .Re – test the sample again using
    a drop of 20μl .
    • The strength of agglutination is not indicative
    of the CRP concentration in the samples
    tested.
    • Clinical diagnosis should not be made on
    findings of a single test result, but should
    integrate both clinical an laboratory data.
    LIMITATIONS

    • Reaction time is critical. If reaction time


    exceeds two minutes, drying of the reaction
    mixture may cause false positive results.
    • Freezing the CRP Latex Reagent will result in
    spontaneous agglutination. phenomenon
    (antigen excess).
    • It is recommended, therefore, to check all
    negative sera by retesting at a 1:10 dilution
    with Saline Solution.

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