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    Tugas Kimia Bahan Alam - Squalene

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    Claudia Natalica
    Grain amaranth is a natural source of squalene, an important component in skin care products and vaccines. Squalene was extracted from Amaranthus sp. seeds through a process involving hexane extraction, saponification using KOH, and vacuum evaporation. The resulting hexane fraction was purified using column chromatography. FTIR and HPLC analysis were used to analyze the squalene content and composition. Supercritical fluid extraction is an alternative green technology that can extract high purity squalene without toxic solvents, but is not economically viable for direct extraction from seeds.

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    Tugas Kimia Bahan Alam - Squalene

    Uploaded by

    Claudia Natalica
    50 views6 pages
    Grain amaranth is a natural source of squalene, an important component in skin care products and vaccines. Squalene was extracted from Amaranthus sp. seeds through a process involving hexane extraction, saponification using KOH, and vacuum evaporation. The resulting hexane fraction was purified using column chromatography. FTIR and HPLC analysis were used to analyze the squalene content and composition. Supercritical fluid extraction is an alternative green technology that can extract high purity squalene without toxic solvents, but is not economically viable for direct extraction from seeds.

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    Tugas Kimia Bahan Alam

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    Grain amaranth is a natural source of squalene, an important component in skin care products and vaccines. Squalene was extracted from Amaranthus sp. seeds through a process involving hexane extraction, saponification using KOH, and vacuum evaporation. The resulting hexane fraction was purified using column chromatography. FTIR and HPLC analysis were used to analyze the squalene content and composition. Supercritical fluid extraction is an alternative green technology that can extract high purity squalene without toxic solvents, but is not economically viable for direct extraction from seeds.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    50 views6 pages

    Tugas Kimia Bahan Alam - Squalene

    Uploaded by

    Claudia Natalica
    Grain amaranth is a natural source of squalene, an important component in skin care products and vaccines. Squalene was extracted from Amaranthus sp. seeds through a process involving hexane extraction, saponification using KOH, and vacuum evaporation. The resulting hexane fraction was purified using column chromatography. FTIR and HPLC analysis were used to analyze the squalene content and composition. Supercritical fluid extraction is an alternative green technology that can extract high purity squalene without toxic solvents, but is not economically viable for direct extraction from seeds.

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    © All Rights Reserved
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    Download as pptx, pdf, or txt
    of 6

    Kimia Bahan Alam

    SQUALENE
    Extraction and Purification from Amaranthus sp.
    Oleh : Claudia Natalica (1400810067)
    Latar Belakang
    Grain amaranth (plant) has been
    suggested as an alternative to marine
    animals as a natural source of squalene

    At the beginning of 1950s it was discovered that squalene is an important


    component of human sebum, a fact that justifies its role in the physiology of
    skin: its action in skin hydration, repairing of the damaged skin, and
    rejuvenating the ageing skin was demonstrated. The emollient and hydration
    properties of squalene and also its biocompatibility with skin make squalene an
    important component in cosmetics formulations (moisturizing creams, makeup,
    lipstick, and nail and hair products)

    Squalene was also used as an adjuvant in vaccines, stimulating the immune


    response and increasing the patient’s response to vaccine. It is added to lipid
    emulsions as drug carrier in vaccine applications. The oil in water emulsions
    containing squalene facilitates solubilization and modifies the release and cell
    uptake of drugs, adjuvants, and vaccines.

    Amaranthus cruentus
    Squalene
    Natural triterpene

    C30H50

    Mevalonic Acid
    Pathway
    Triterpenes are not formed by an
    extension of the now
    familiar process of adding IPP to the
    growing chain. Instead,
    two molecules of FPP are joined tail-to-
    tail to yield squalene
    Metode

    Ekstraksi dengan Vakum


    Saponifikasi Fraksinasi
    heksana Evaporasi
    Ekstraksi minyak dari Menggunakan larutan Fraksi heksana Fraksi yang tidak
    biji Amarathus lebih KOH 25% di dalam dikeringkan dengan tersabunkan kemudian
    sering dilakukan etanol 96%, pada suhu menggunakan dimurnikan dengan
    karena dimensinya 50ºC sampai dengan evaporasi vakum pada kromatografi kolom
    yang kecil (luas 55ºC selama 30 menit. suhu 50ºC dengan fase gerak
    permukaan besar) dan Campuran reaksi heksana dan fase diam
    kulit biji tidak tebal kemudian diekstrak silika gel 60 (size 60Å,
    sehingga biji cukup menggunakan heksana 70-230 mesh, 63-200 μm)
    dihancurkan/digiling dan dicuci dengan
    aquades.
    Metode Analisa Hasil green extraction technology

    Supercritical Fluid Extraction (SFE)  inertness,


    nontoxicity, high volatility, and low cost.

    FTIR HPLC + the high purity of the end product


    + short processing time due to the fact that
    extraction and concentration are carried out in
    Fourier Transform Infra Digunakan untuk one step.
    Red (FTIR) digunakan mengukur kandungan + yields high quality squalene without use of toxic
    untuk menganalisis squalene berdasarkan unallowable solvents.
    kandungan gugus standar yang sudah
    fungsi pada hasil ada. - not economically effective to use SFE for direct
    pemisahan extraction of squalene from seeds; it is suitable
    only for the extraction of squalene from the plant
    oil.
    - expensive cost of the equipment
    Referensi

    [1] Nuryanto et al. 2016. Isolasi Squalen dari Asam Lemak Sawit Distilat (ALSD). J.
    Kimia dan Kemasan. Vol 38. No 2. 55-60

    [2] He, H. et al. 2002. Extraction and Purification of Squalene from Amaranthus Gran. J.
    Agric. Food Chem. 50 (2). 368-372

    [3] Ovidiu Popa, Narcisa Elena Băbeanu, Ioana Popa, Sultana Niță, and Cristina Elena
    Dinu-Pârvu, “Methods for Obtaining and Determination of Squalene from Natural
    Sources,” BioMed Research International, vol. 2015, Article ID 367202, 16 pages,
    2015. doi:10.1155/2015/367202

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