The Seidel test uses fluorescein dye to identify leaks from the eye following injury or surgery. Aqueous fluid from inside the eye normally cannot be seen when it leaks due to the tear film covering the eye. The Seidel test applies fluorescein dye to the tear film so that any leaking aqueous fluid will dilute the dye and make the leak visible under cobalt blue light on a slit lamp examination. A positive test shows bright green dye concentrated around the leak site, indicating a leak that requires further management.
The Seidel test uses fluorescein dye to identify leaks from the eye following injury or surgery. Aqueous fluid from inside the eye normally cannot be seen when it leaks due to the tear film covering the eye. The Seidel test applies fluorescein dye to the tear film so that any leaking aqueous fluid will dilute the dye and make the leak visible under cobalt blue light on a slit lamp examination. A positive test shows bright green dye concentrated around the leak site, indicating a leak that requires further management.
The Seidel test uses fluorescein dye to identify leaks from the eye following injury or surgery. Aqueous fluid from inside the eye normally cannot be seen when it leaks due to the tear film covering the eye. The Seidel test applies fluorescein dye to the tear film so that any leaking aqueous fluid will dilute the dye and make the leak visible under cobalt blue light on a slit lamp examination. A positive test shows bright green dye concentrated around the leak site, indicating a leak that requires further management.
The Seidel test uses fluorescein dye to identify leaks from the eye following injury or surgery. Aqueous fluid from inside the eye normally cannot be seen when it leaks due to the tear film covering the eye. The Seidel test applies fluorescein dye to the tear film so that any leaking aqueous fluid will dilute the dye and make the leak visible under cobalt blue light on a slit lamp examination. A positive test shows bright green dye concentrated around the leak site, indicating a leak that requires further management.
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SEIDELS TEST
D R . FA H R E Z A DEFINITION
The Seidel test is named after the German
ophthalmologist Erich Seidel The test used to reveal ocular leaks from the cornea, sclera or conjunctiva following injury or surgery and sometimes disease is called Seidel test. PRINCIPLE
Aqueous in the anterior chamber is a clear fluid. Tear film
covering the conjunctiva and cornea is also a transparent layer. Following ocular injury or surgery or any disease, if ocular leak is present then this fluid will leak out of the eye. However due to tear film, this aqueous leak disappears and site of the leak cannot be identified. Thus, if the dye were injected into aqueous then the site of leak can be identified by the dye leaking out of the wound. However this is an invasive procedure, so non-invasive alternative would obviously be preferable and an alternative approach is the seidel test dye the tear film. Any aqueous leak will dilute the dyed tears in the site of leak and the leak would be much more obvious by color change. DYE USED IN SEIDEL TEST
Fluorescein 10 % (Resorcinolphthalein) dye is used in
the seidel test. Fluorescein is a synthetic organic compound available as a dark orange or red in color. It can be prepared from phthalic anhydride and resorcinol in the presence of zinc chloride via the Friedel-Crafts reaction. As concentrated fluorescein does not actually fluoresce, it is used in relatively dilute concentrations in most circumstances. Concentrated fluorescein will be diluted by the leak which will flow downward due to gravity and on slit lamp examination by using cobalt blue light this leak will appear bright green. TECHNIQUE
Prepare the slit lamp
Explain the procedure to the patient Gently apply the topical anesthetic eye drop Carefully apply moistened Fluorescein dye strip to the superior conjunctiva. (In corneal tear, the dye can usually be applied to the superior conjunctiva, allowing the dye to flow down over the cornea. Another advantage of applying fluorescein to the superior conjunctiva above the suspected lesion is that Bells phenomenon whereby the eyes rotate upward and outward.) Ask patient to blink once Visualize the injured site under cobalt blue light source Estimate the rate and volume of fluid exiting the wound Apply topical antibiotic eye drop INDICATIONS
(1) To identify the site of ocular leak after globe injury
(2) To identify whether the corneal tear is sealed or not (3) To evaluate different sutureless cataract incision configurations (4) Post-operatively to ensure appropriate wound closure after filtering bleb CONTRAINDICATIONS
(1) Obvious Globe rupture
(2) Corneal perforation (3) Full thickness eye laceration (4) Hypersensitivity to Fluorescein INTERPRETATION (POSITIVE SEIDEL TEST) A. Fluorescein dye diluted by aqueous fluid B. Bright green concentrated dye surrounds leak site (above and to side PRECAUTIONS
Do not apply pressure to eye during test (risk of eye
tissue extrusion) REQUIREMENTS
A. Slit lamp with cobalt blue light source
B. Fluorescein strip C. Topical Anesthetic eye-drops MANAGEMENT
A full thickness penetration (leaking wound) should be
treated as an emergency and require prompt surgical repair. Care must be taken to protect the eye. A sealed corneal tear (small wound) is treated by applying bandage contact lens or pressure patching. Any inadequate wound closure or incision closure needs to be reviewed. Cyanoacrylate glue and a bandage contact lens may be applied to close the wound. Broad-spectrum antibiotics are indicated to decrease any chance of microbial keratitis or endophthalmitis. Tetanus prophylaxis is given. PROGNOSIS
Depends on the injuries sustained and the extent of