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    Chap 6 Microbial Nutrition and Growth Fall 2012

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    The document discusses microbial nutrition, growth, and methods of measuring growth. It addresses the requirements for microbial growth including necessary nutrients, sources of nutrients, and different types of associations between microbes. It also describes the typical growth curve of microbes including lag phase, log/exponential phase, stationary phase, and death phase. Finally, it outlines several direct and indirect methods for measuring microbial reproduction and growth, such as plate counts, turbidity, and genetic techniques.

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    Chap 6 Microbial Nutrition and Growth Fall 2012

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    joiesupremo
    62 views21 pages
    The document discusses microbial nutrition, growth, and methods of measuring growth. It addresses the requirements for microbial growth including necessary nutrients, sources of nutrients, and different types of associations between microbes. It also describes the typical growth curve of microbes including lag phase, log/exponential phase, stationary phase, and death phase. Finally, it outlines several direct and indirect methods for measuring microbial reproduction and growth, such as plate counts, turbidity, and genetic techniques.

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    The document discusses microbial nutrition, growth, and methods of measuring growth. It addresses the requirements for microbial growth including necessary nutrients, sources of nutrients, and different types of associations between microbes. It also describes the typical growth curve of microbes including lag phase, log/exponential phase, stationary phase, and death phase. Finally, it outlines several direct and indirect methods for measuring microbial reproduction and growth, such as plate counts, turbidity, and genetic techniques.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    62 views21 pages

    Chap 6 Microbial Nutrition and Growth Fall 2012

    Uploaded by

    joiesupremo
    The document discusses microbial nutrition, growth, and methods of measuring growth. It addresses the requirements for microbial growth including necessary nutrients, sources of nutrients, and different types of associations between microbes. It also describes the typical growth curve of microbes including lag phase, log/exponential phase, stationary phase, and death phase. Finally, it outlines several direct and indirect methods for measuring microbial reproduction and growth, such as plate counts, turbidity, and genetic techniques.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 21

    Chapter 6

    Microbial
    Nutrition and
    Growth
    Growth Requirements

    Microbial growth
    Increase in a population of microbes
    Result of microbial growth is discrete colony
    An aggregation of cells arising from single parent
    cell
    Reproduction results in growth

    7/6/11 MDufilho 2
    Growth Requirements

    Organisms use a variety of nutrients for


    their energy needs and to build organic
    molecules and cellular structures
    Most common nutrients contain
    necessary elements such as carbon,
    oxygen, nitrogen, and hydrogen
    Microbes obtain nutrients from variety of
    sources

    7/6/11 MDufilho 3
    Growth Requirements

    Associations
    Organisms live in association with different species
    Antagonistic relationships
    Synergistic relationships
    Symbiotic relationships

    7/6/11 MDufilho 4
    Growth Requirements

    Biofilms
    Complex relationships among numerous
    microorganisms
    Develop an extracellular matrix
    Adheres cells to one another
    Allows attachment to a substrate
    Sequesters nutrients
    May protect individuals in the biofilm
    Form on surfaces often as a result of quorum
    sensing
    Many microorganisms more harmful as part of a
    biofilm
    7/6/11 MDufilho 5
    Figure 6.17 Binary fission events-overview

    7/6/11 MDufilho 6
    Growth of Microbial Populations

    ANIMATION Binary Fission

    7/6/11 MDufilho 7
    Figure 6.18 Comparison of arithmetic and logarithmic growth-overview

    7/6/11 MDufilho 8
    Growth of Microbial Populations

    Generation Time
    Time required for a bacterial cell to grow
    and divide
    Dependent on chemical and physical
    conditions

    7/6/11 MDufilho 9
    Figure 6.19 Two growth curves of logarithmic growth-overview

    7/6/11 MDufilho 10
    Figure 6.20 Typical microbial growth curve

    Stationary phase
    Number of live cells (log)

    Death
    (decline)
    Log phase
    (exponential)
    phase

    Lag phase

    Time

    7/6/11 MDufilho 11
    Figure 6.21 Schematic of chemostat
    Fresh medium with
    a limiting amount Flow-rate
    of a nutrient regulator

    Sterile air
    of other
    gas
    Culture
    vessel

    Culture Overflow
    tube

    7/6/11 MDufilho 12
    Growth of Microbial Populations

    Measuring Microbial Reproduction


    Direct methods
    Serial dilution and viable plate counts
    Membrane filtration
    Most probable number
    Microscopic counts
    Electronic counters

    7/6/11 MDufilho 13
    Figure 6.22 Estimating microbial population size-overview

    7/6/11 MDufilho 14
    Figure 6.23 Use of membrane filtration to estimate microbial population-overview

    7/6/11 MDufilho 15
    Figure 6.24 The most probable number (MPN) method for estimating microbial numbers

    1.0 ml 1.0 ml

    Undiluted 1:10 1:100

    Inoculate 1.0 ml into


    each of 5 tubes

    Phenol red, pH
    color indicator,
    added

    Incubate

    Results

    7/6/11
    4 tubes positiveMDufilho
    2 tubes positive 1 tube positive 16
    Figure 6.25 The use of a cell counter for estimating microbial numbers-overview

    7/6/11 MDufilho 17
    Growth of Microbial Populations

    Measuring Microbial Growth


    Indirect methods
    Metabolic activity
    Dry weight
    Turbidity

    7/6/11 MDufilho 18
    Figure 6.26 Spectrophotometry-overview

    7/6/11 MDufilho 19
    Growth of Microbial Populations

    Measuring Microbial Reproduction


    Genetic methods
    Isolate DNA sequences of unculturable
    prokaryotes
    Used to estimate the number of these
    microbes

    7/6/11 MDufilho 20
    Case StudyCan a trip to the dentist be life threatening?

    Details of the case


    Why do we get our teeth cleaned?
    Why does Betty feel so bad?
    What has happened?
    Will the biofilm return?
    How did they figure out Betty had septicemia?

    7/6/11 MDufilho 21

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