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    Bromine Water Test

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    annespeaks017
    This document describes two tests to identify unsaturated lipids and the presence of glycerol. The bromine water test uses bromine solution, which will decolorize in the presence of double bonds, to test vegetable oil and lecithin, both of which were unsaturated. The acrolein test uses heat and potassium bisulfate to dehydrate glycerol into acrolein, producing a burnt odor, which was observed for glycerol, vegetable oil, and lecithin, identifying them as glycerides.

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    Bromine Water Test

    Uploaded by

    annespeaks017
    3K views22 pages
    This document describes two tests to identify unsaturated lipids and the presence of glycerol. The bromine water test uses bromine solution, which will decolorize in the presence of double bonds, to test vegetable oil and lecithin, both of which were unsaturated. The acrolein test uses heat and potassium bisulfate to dehydrate glycerol into acrolein, producing a burnt odor, which was observed for glycerol, vegetable oil, and lecithin, identifying them as glycerides.

    Original Description:

    Bromine Water Test (Lipids)

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    © © All Rights Reserved

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    This document describes two tests to identify unsaturated lipids and the presence of glycerol. The bromine water test uses bromine solution, which will decolorize in the presence of double bonds, to test vegetable oil and lecithin, both of which were unsaturated. The acrolein test uses heat and potassium bisulfate to dehydrate glycerol into acrolein, producing a burnt odor, which was observed for glycerol, vegetable oil, and lecithin, identifying them as glycerides.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    3K views22 pages

    Bromine Water Test

    Uploaded by

    annespeaks017
    This document describes two tests to identify unsaturated lipids and the presence of glycerol. The bromine water test uses bromine solution, which will decolorize in the presence of double bonds, to test vegetable oil and lecithin, both of which were unsaturated. The acrolein test uses heat and potassium bisulfate to dehydrate glycerol into acrolein, producing a burnt odor, which was observed for glycerol, vegetable oil, and lecithin, identifying them as glycerides.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 22

    BROMINE WATER TEST

    By: Anne Marielle D. Manalo

    Objective:
    To test for the presence
    of unsaturation in a fatty
    acid or a triglyceride.

    Results:
    Sample

    Result

    Vegetable Oil

    Colorless

    Lecithin

    Colorless

    Principle:
    The presence of unsaturation
    in a fatty acid or a triacylglycerol
    can be detected by reaction with
    bromine. If the orange color of a
    bromine solution added to a lipid
    fades
    quickly,
    an
    addition
    reaction has occurred and the oil
    or fat is unsaturated.

    Type Equation Used:

    Sample Equation Involved:


    Vegetable Oil:

    +
    (orange)
    Br
    2
    (colorless)

    Sample Equation Involved:


    Lecithin:

    +
    (orange)
    Br
    2

    (colorless)

    Explanation of Results:
    Why does vegetable oil showed
    positive results?
    Vegetable oil is a triglyceride extracted from a plant
    and is liquid in room temperature. It contains double
    carbon-carbon bonds making it unsaturated. Bromine
    readily add up at the double bonds, and a colored
    solution of bromine when absorbed and decolorized in
    the presence of a lipid, indicates the presence of
    double bond. The more double bonds there are, the

    Explanation of Results:
    Why does lecithin showed
    positive
    Lecithinis aresults?
    generic term to designate any group
    of yellow-brownish fatty substances occurring in
    animal and plant tissues. Bromine readily add up at
    the double bond of the unsaturated fatty acid, and a
    colored solution of bromine when absorbed and
    decolorized in the presence of a lipid, indicates the
    presence of double bond.

    ACROLEIN TEST

    Objective:
    To test for the
    presence of glycerol.

    Procedure:
    1. Prepare 3 clean and dry test
    tubes

    1st test
    tube: Add a
    drop of
    glycerol.

    2nd test
    tube: Add a
    drop of
    cooking oil

    3rd test tube:


    Add a drop of
    lecithin from
    the soft gel
    capsule.

    Procedure:
    2. Mix a pinch-sized amount of
    powdered potassium bisulfate
    to all the tubes.

    Procedure:
    3. Heat the mixture gently over a small
    flame. Note the odor of the vapor and
    record observations.

    Results:

    SAMPLE

    ODOR

    Glycerol

    Burnt smell

    Cooking oil

    Burnt smell

    Lecithin

    Burnt smell

    Principle:
    When a fat is heated strongly in
    the presence of a dehydrating agent
    such as potassium bisulfate (KHSO),
    the glycerol portion of the molecule
    is
    dehydrated
    to
    form
    the
    unsaturated aldehyde, acrolein
    (CH2=CHCHO), which has the
    peculiar odor of burnt grease.

    Sample Equation Involved:


    a. Glycerol and Potassium bisulfate

    Sample Equations Involved:


    b. Vegetable oil and Potassium bisulfate
    H+
    + 3H2O

    KHSO
    4

    glycerol

    Sample Equation Involved:


    c. Lecithin and Potassium bisulfate

    What is the compound


    responsible for your
    observation?
    The heat-induced
    dehydration

    of
    glycerol is the one responsible for the
    release of acrolein.
    Acrolein
    is
    the
    compound
    responsible for the burnt smell.

    What is the purpose of the


    potassium bisulfate used?
    When lipids are heated in the presence of a
    dehydrating

    agent

    such

    as

    potassium

    bisulfate (KHSO4), the glycerol portion of the


    molecule

    is

    dehydrated

    to

    unsaturated aldehyde, acrolein.

    form

    the

    What group of lipids was identified


    by this test?

    Glycerides was the group of


    lipids identified by this test.

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