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    Lab Exericise 3 For Microscope

    Uploaded by

    Khin Aung Shwe
    This document provides instructions for several microscope exercises in Anatomy and Physiology I. It details how to properly care for and use a compound microscope, including holding it upright and cleaning lenses with grit-free paper. It describes focusing on the lowest power objective first and using wet mount preparations with a coverslip. The document explains microscope parts, how magnification is calculated from the ocular and objective lenses, and estimating field diameter. It provides steps for perceiving depth of field and preparing a wet mount specimen from a cheek scraping under the microscope.

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    © All Rights Reserved
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    Lab Exericise 3 For Microscope

    Uploaded by

    Khin Aung Shwe
    242 views9 pages
    This document provides instructions for several microscope exercises in Anatomy and Physiology I. It details how to properly care for and use a compound microscope, including holding it upright and cleaning lenses with grit-free paper. It describes focusing on the lowest power objective first and using wet mount preparations with a coverslip. The document explains microscope parts, how magnification is calculated from the ocular and objective lenses, and estimating field diameter. It provides steps for perceiving depth of field and preparing a wet mount specimen from a cheek scraping under the microscope.

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    Microscope

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    Lab Exericise 3 for Microscope

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    This document provides instructions for several microscope exercises in Anatomy and Physiology I. It details how to properly care for and use a compound microscope, including holding it upright and cleaning lenses with grit-free paper. It describes focusing on the lowest power objective first and using wet mount preparations with a coverslip. The document explains microscope parts, how magnification is calculated from the ocular and objective lenses, and estimating field diameter. It provides steps for perceiving depth of field and preparing a wet mount specimen from a cheek scraping under the microscope.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    242 views9 pages

    Lab Exericise 3 For Microscope

    Uploaded by

    Khin Aung Shwe
    This document provides instructions for several microscope exercises in Anatomy and Physiology I. It details how to properly care for and use a compound microscope, including holding it upright and cleaning lenses with grit-free paper. It describes focusing on the lowest power objective first and using wet mount preparations with a coverslip. The document explains microscope parts, how magnification is calculated from the ocular and objective lenses, and estimating field diameter. It provides steps for perceiving depth of field and preparing a wet mount specimen from a cheek scraping under the microscope.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 9

    Anatomy and Physiology I (BIO130)

    Spring 2010

    Prof. AnnMarie Armenti, MS

    Exercise 3:
    The Microscope
    Care of the Compound Microscope
    Hold in an upright position with one hand on the arm
    and the other supporting the base
    Use grit-free lens paper to clean the lenses. Clean all
    lenses before and after use
    Begin focusing on the lowest power objective
    Use the coarse adjustment knob only with the lowest
    power lens
    Always use a coverslip with wet mount preparation
    Before returning the microscope:
    Remove the slide from the stage
    Rotate the lowest-power objective lens into position
    Replace the dust cover or return it to the storage area

    Exercise 3:
    The Microscope
    Activity 1: Identifying the Parts of the Microscope
    Activity 2: Viewing Objects through the Microscope
    Activity 3: Estimating the Diameter of the
    Microscope Field

    Activity 4: Perceiving Depth


    Activity 5: Preparing and Observing a Wet Mount

    (10x)

    Head (support)

    Rotating Nosepiece

    Iris Diaphragm Lever


    (regulates the light passing
    through the condenser contrast)

    (support)

    Condenser
    (Small lens that concentrates
    The light on the specimen)

    (specimen)

    (precise focusing)

    Exercise 3:
    The Microscope
    Magnification
    Interplay of 2 lenses
    Ocular Lens (10x) Virtual image that we see
    Objective Lens (4x, 10x, and 40x) Real image

    Total Magnification:
    Power of Ocular Lens x the power of Objective Lens
    10 x 4 = 40x

    Field: the area seen through the microscope

    Exercise 3:
    The Microscope
    Microscope Field
    Size of the microscope field decreases with
    increasing magnification
    Determining the diameter of the field
    Field diameter = 4mm
    Object extends across half the field
    4 = 2 mm

    Remember: Start lowest-power objective


    to bring the grid lines into focus

    Exercise 3:
    The Microscope
    Perceiving Depth
    Depth of the Field (depth of the specimen clearly in focus)
    is greater at lower magnification

    1. Use Iris diaphragm lever to reduce the light


    Increase contrast

    2. Focus with the coarse adjustment until threads


    are out of focus
    3. Slowly focus them again, note which thread
    comes first

    Exercise 3:
    The Microscope
    Preparing a Wet Mount
    1.
    2.
    3.
    4.
    5.
    6.

    Place a drop of saline in the center of slide


    Rub the inside lining of your cheek
    Transfer cheek scrapings into saline on slide
    Add a tiny drop of iodine or methylene blue
    Stir
    Coverslip: prevents soiling of the lens if you misfocus
    Bottom edge should touch one side of the fluid drop
    Then carefully lower the coverslip.

    *** Probably want to dim the light with the iris diaphragm
    Increase Contrast

    How do you Prepare for Next Week?


    Lecture:
    Read Chapter 1 and 2 in the Text
    Read over handouts

    Laboratory:
    Finish Lab. Exercise 1 and 3
    Read Lab. Exercises 4 and 5A

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