The document provides information about cells and how to use a microscope:
1. It defines what a cell is and where cells are found, such as in plants, animals, and microorganisms but not in non-living things.
2. It describes the proper procedure for using a microscope, including adjusting the light, placing the slide, focusing with the coarse and fine knobs, and returning the objective lens when finished.
3. It explains how to prepare slides by placing specimens and cover slips, as well as techniques for coloring specimens, and provides tips for sketching cells observed under the microscope.
Copyright:
Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPT, PDF, TXT or read online from Scribd
The document provides information about cells and how to use a microscope:
1. It defines what a cell is and where cells are found, such as in plants, animals, and microorganisms but not in non-living things.
2. It describes the proper procedure for using a microscope, including adjusting the light, placing the slide, focusing with the coarse and fine knobs, and returning the objective lens when finished.
3. It explains how to prepare slides by placing specimens and cover slips, as well as techniques for coloring specimens, and provides tips for sketching cells observed under the microscope.
The document provides information about cells and how to use a microscope:
1. It defines what a cell is and where cells are found, such as in plants, animals, and microorganisms but not in non-living things.
2. It describes the proper procedure for using a microscope, including adjusting the light, placing the slide, focusing with the coarse and fine knobs, and returning the objective lens when finished.
3. It explains how to prepare slides by placing specimens and cover slips, as well as techniques for coloring specimens, and provides tips for sketching cells observed under the microscope.
Copyright:
Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPT, PDF, TXT or read online from Scribd
The document provides information about cells and how to use a microscope:
1. It defines what a cell is and where cells are found, such as in plants, animals, and microorganisms but not in non-living things.
2. It describes the proper procedure for using a microscope, including adjusting the light, placing the slide, focusing with the coarse and fine knobs, and returning the objective lens when finished.
3. It explains how to prepare slides by placing specimens and cover slips, as well as techniques for coloring specimens, and provides tips for sketching cells observed under the microscope.
Copyright:
Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPT, PDF, TXT or read online from Scribd
Download as ppt, pdf, or txt
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Chapter 2: Cell
2.1 What is a cell?
1. Cell is the basic unit of life 2. Where does the cell is find? Cell is find in living thing such as human, plant e.g.hibiscus,…………………………….., animal e.g. fish, ……………………………….. microorganism e.g.bacteria,…………………………. 3. Cell is not find in non living thing such as ……………………………….. 4. Cell can be seen under ………….. Part of microscope Parts & function of microscope part function 1.
3. How to use microscope 1. Adjust mirror so the appropriate amount of light passes through the hole in the stage. 2. Place the glass slide onto the stage.
3. While watching from the side, use the
coarse focus knob to lower the objective lenses until coarse knob are just above the stage. Pressing focus knob down too far may shatter the slide. 4. While looking through the eyepiece lens, carefully turn the coarse focus knob until the specimen is seen clearly.
5. Carefully use the fine focus knob so that
the details of specimen can be seen as clearly as possible. Important reminders when using a microscope 1. When lifting the microscope, put one hand on the body/arm of the microscope and one hand under its base. 2. The microscope should be used on a flat surface and not too close to the edge. 3. Take care that the light intensity is not too high, or it might damage the eye. 4. Return the shortest/smallest objective lens into position when finished.
5. Remove the slide, and ensure that
the stage is clean. 6. Make sure that when microscope is not in use, it is always clean and carefully put away. Preparing slide 1. Put a drop of water on clean slide and stain (e.g. iodine) if the specimen is dry specimen
2. Place the specimen on the slide by using
i….. ii…… iii……. 3. Cover specimen with cover slip by using ……….. gently Incorrect placing of the cover slip can result in air bubbles. 4. Another technique to color the specimen is draining technique. • Draining technique 1. Put iodine drops at one end of cover slip 2. Place a filter paper at the opposite end of cover slip Return to • An example of a sketch from a microscope specimen step 5 how to sketch Sketching cell under the microscope 1. Use a sharp lead pencil. 2. Draw only the lines that can be seen (no shading or colouring). 3. Diagrams should take up about a third to half a page each. 4.Record the magnification next to each diagram. 5. State the name of the specimen and the date of observation. 6. Written description/label is also often of considerable value. 7. If there are many viewing cells at one time, draw only two or three representative cells for each observation. magnification • Multiplying the eyepiece number by the objective lens number will give the magnification of the microscope. For example: • eyepiece: x 10 • objective: x 40 • magnification = x 400 Return to how to sketch Field of view Use the ‘Field of view’ diagrams to answer the following questions. (1000µm = 1 mm) field of view 1.6 mm (a) Estimate the length of magnification x 100 the specimen shown in the diagram at x100 and x400 magnification. (b) If a specimen is 1 mm in length, how big will it appear if it is magnified field of view 400µm x100? magnification x 400 • Return to how to sketch step 5