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    Real World: Determination of Calcium in Milk: by Delaney Caudill, Ethan Nichols, and Katie Vautier

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    kasun1237459
    This document details an experiment to determine the calcium content of different types of milk using complexometric titration. Two indicators, Patton-Reeder and Eriochrome Black T, were used to detect the endpoint of the titration. Calcium standards were prepared and titrated against EDTA to obtain calibration curves. Milk samples were then titrated and calcium content calculated. The calcium values obtained were compared to listed values and to each other. The Patton-Reeder method yielded lower percentage differences than Eriochrome Black T, likely due to pH effects on the indicator. Future work could optimize the method using Eriochrome Black T.

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    Real World: Determination of Calcium in Milk: by Delaney Caudill, Ethan Nichols, and Katie Vautier

    Uploaded by

    kasun1237459
    72 views23 pages
    This document details an experiment to determine the calcium content of different types of milk using complexometric titration. Two indicators, Patton-Reeder and Eriochrome Black T, were used to detect the endpoint of the titration. Calcium standards were prepared and titrated against EDTA to obtain calibration curves. Milk samples were then titrated and calcium content calculated. The calcium values obtained were compared to listed values and to each other. The Patton-Reeder method yielded lower percentage differences than Eriochrome Black T, likely due to pH effects on the indicator. Future work could optimize the method using Eriochrome Black T.

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    about calculating ca concentration of milk

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    Real World Powerpoint

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    This document details an experiment to determine the calcium content of different types of milk using complexometric titration. Two indicators, Patton-Reeder and Eriochrome Black T, were used to detect the endpoint of the titration. Calcium standards were prepared and titrated against EDTA to obtain calibration curves. Milk samples were then titrated and calcium content calculated. The calcium values obtained were compared to listed values and to each other. The Patton-Reeder method yielded lower percentage differences than Eriochrome Black T, likely due to pH effects on the indicator. Future work could optimize the method using Eriochrome Black T.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    72 views23 pages

    Real World: Determination of Calcium in Milk: by Delaney Caudill, Ethan Nichols, and Katie Vautier

    Uploaded by

    kasun1237459
    This document details an experiment to determine the calcium content of different types of milk using complexometric titration. Two indicators, Patton-Reeder and Eriochrome Black T, were used to detect the endpoint of the titration. Calcium standards were prepared and titrated against EDTA to obtain calibration curves. Milk samples were then titrated and calcium content calculated. The calcium values obtained were compared to listed values and to each other. The Patton-Reeder method yielded lower percentage differences than Eriochrome Black T, likely due to pH effects on the indicator. Future work could optimize the method using Eriochrome Black T.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 23

    Real World:

    Determination of
    Calcium in Milk

    By Delaney Caudill, Ethan Nichols,


    and Katie Vautier

    Background
    Methods Considered
    Ion Selective Electrode Method
    Trichloroacetic Acid Filtrate Method
    Use of this acid as a protein precipitator
    to determine calcium content

    Complexometric Titrations
    A volumetric analysis in which a
    formation of a colored complex is used to
    indicate the end point of a titration

    Purpose
    Determine and compare the calcium
    content via a complexometric
    titration to listed values of different
    types of milk
    Compare two different indicators

    Procedure #1: PattonReeder


    Dry EDTA at 80C overnight
    Make 750mL of 0.03408M EDTA
    Combine 10mL of sample, 40mL
    distilled water, and 4mL of 8M
    sodium hydroxide solution into an
    Erlenmeyer flask
    Add 0.1g of Patton-Reeder indicator
    Titrate w/ EDTA solution

    Data
    Whole Milk
    Trial

    mL EDTA added

    Grams Ca2+

    9.72

    0.013276

    9.47

    0.012935

    9.21

    0.012580

    9.39

    0.012825

    Avg.

    ----------

    0.012904

    % Difference

    Fat free milk: 5.1%


    1% milk: 2.5%
    2% milk: 2.0%
    Whole milk: 6.2%
    Calcium Fortified: 9.0%

    Before Titration

    After Titration

    Color Change?

    Procedure #2: Eriochrome


    Black T
    Add 3mL of milk and 5mL ammonia
    buffer in a 250mL Erlenmeyer flask
    Calibrate a pH meter and measure
    pH of each sample (needs to be 10)
    Add 0.03g of indicator
    Titrate with EDTA

    Data
    Whole Milk
    Trial

    mL EDTA added

    Grams Ca2+

    5.10

    0.0069659

    4.48

    0.0061190

    4.41

    0.0060234

    4.50

    0.0061464

    Avg.

    ----------

    0.0063137

    Results
    Whole milk: 54.1%
    Makes sense due to different
    indicator (EBT)
    pH of sample was 10
    pH had effect on sample?

    Procedure #3: Eriochrome


    Black T

    Same as procedure #1 except used


    different indicator: Eriochrome Black
    T

    Data & Results


    No quantitative data
    No color change due to the solution
    having a pH between 12 and 14
    Eriochrome Black T indicates a color
    change at a pH of 10

    No Color Change

    Theoretical Grams of Ca per Serving


    Based on a 2,000 calorie diet, daily
    calcium intake should be 1,100mg
    Each milk carton claims it contains
    30% of daily calcium intake

    Example Calculations
    Grams Ca2+ in a serving of milk

    Ex. Whole Milk

    Percent Difference

    Ex. Whole Milk

    Sources of Error
    INDICATORS!
    Subjective color change

    Different people titrated samples


    Reading the buret
    Wait for precipitate to come out of
    solution to remove magnesium
    oxide

    Future Work
    Investigate different indicators
    Change pH of samples to use
    Eriochrome Black T indicator by
    changing molarity of NaOH
    Try other calcium supplements such
    as vitamins or orange juice

    Conclusion
    Successful experiment
    Efficient method: complexometric
    titration
    Low percent differences

    Necessary to order Patton Reeder


    since Eriochrome Black T yielded
    higher percent difference

    References
    Harris, Daniel C. Quantitative
    Chemical Analysis, 8th ed.; W.H.
    Freeman and Company: New York,
    2010, page 240.
    http://www.chemteach.ac.nz/invesiga
    tions/documents/calcium.pdf
    http://www.jbc.org/content/90/3/747.f
    ull.pdf

    Special Thanks
    Dr. Peterman
    Melissa, Kate, Pat, and
    Kiersten
    Professor Mowery

    Question
    s?

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