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    Recovery and Purification of Products

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    Ratih Margita Sari
    This document discusses the recovery and purification processes for fermentation products. Recovery and purification can account for over 50% of manufacturing costs, especially for intracellular products like citric acid. The processes typically involve four primary functions: 1) separating insoluble product and solids, 2) primary isolation or concentration of the product, 3) purification by removing contaminants, and 4) product preparation such as drying. Common separation methods include filtration, centrifugation, and coagulation/flocculation to remove biomass, cells, and insoluble particles from the fermentation broth in the initial recovery stages.

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    Recovery and Purification of Products

    Uploaded by

    Ratih Margita Sari
    102 views15 pages
    This document discusses the recovery and purification processes for fermentation products. Recovery and purification can account for over 50% of manufacturing costs, especially for intracellular products like citric acid. The processes typically involve four primary functions: 1) separating insoluble product and solids, 2) primary isolation or concentration of the product, 3) purification by removing contaminants, and 4) product preparation such as drying. Common separation methods include filtration, centrifugation, and coagulation/flocculation to remove biomass, cells, and insoluble particles from the fermentation broth in the initial recovery stages.

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    Recovery and Purification of Products

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    This document discusses the recovery and purification processes for fermentation products. Recovery and purification can account for over 50% of manufacturing costs, especially for intracellular products like citric acid. The processes typically involve four primary functions: 1) separating insoluble product and solids, 2) primary isolation or concentration of the product, 3) purification by removing contaminants, and 4) product preparation such as drying. Common separation methods include filtration, centrifugation, and coagulation/flocculation to remove biomass, cells, and insoluble particles from the fermentation broth in the initial recovery stages.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    102 views15 pages

    Recovery and Purification of Products

    Uploaded by

    Ratih Margita Sari
    This document discusses the recovery and purification processes for fermentation products. Recovery and purification can account for over 50% of manufacturing costs, especially for intracellular products like citric acid. The processes typically involve four primary functions: 1) separating insoluble product and solids, 2) primary isolation or concentration of the product, 3) purification by removing contaminants, and 4) product preparation such as drying. Common separation methods include filtration, centrifugation, and coagulation/flocculation to remove biomass, cells, and insoluble particles from the fermentation broth in the initial recovery stages.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 15

    Is essential to any commercial process .

    Products may be biomass it self, an


    extracellular component, or an intracellular
    component.
    Recovery and purification are major expensess
    in production of most fermentation product.
    Comprise more than 50% of the total
    manufacturing cost,especially for an
    intracellular products. For example: citric
    acid.
    The efficiency of the product process and the
    dificulty and cost of the recoveryand
    purification processes are tigthly coupled.
    1. Fermentation
    2. Cell removal and concentration
    3. Cell disruptiuon
    4. Removal of cell debris
    5. Protein precipitation or aqueoust wo-phase
    extraction
    6. Ultrafiltration
    7. Cromatographic purification
    8. Solvent precipitation
    9. Dialysis
    10. Lyophilization
    In the step above, can be generalized to involve
    four primary function:
    1. Separation of insoluble product and other
    solids.
    2. Primary isolation or concentration of product
    and removal of most of the water.
    3. Purification or removal contaminating
    chemical.
    4. Product preparation such as drying.
    Separation of Insoluble Products
    The separation of solid such as biomass
    insoluble particles and macromolecules from
    the fermentation broth is usually the first step
    in product recovery. For examples of
    pretreatment are heat treatment, pH and
    ionic strength adjustment, and the adition of
    coagulans and flocculants.
    The major methods
    1. Filtration (both rotary vacuum filtration and
    micro- or ultrafiltration)
    2. Sentrifugation
    3. Coagulation and flocculation.
    Filtration
    Is probably the most cost-efective method for
    the separationoflarge solid particles and cells
    from fermentation broth.
    Continous rotary filters or rotary vacuum
    precoat filters are the most widly used types
    in the fermentation industry

    Filtration is commonly used for separating my
    celium from fermentation broth in anti biotic
    fermentations. It is also commonly used in
    waste-water treatment facilities.


    Centrifugation
    Is used to separate particles of size between
    100 and 0,1 m from liquid by centrifugal
    process. The major forces acting on a solid
    particle settling in a liquid by gravitational
    forces are gravitational force ( Fg), drag force (
    Fd), and buoyant force ( Fb)
    Value
    Fg = Fd + Fb
    Where

    Coagulation And Flocculation
    Are usually used to form cell aggregates
    before centrifugation, grafity settling, or
    filtration to improve deperformance of these
    separation processes.

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