Introduction To Biostatistics

1
Chapter 1

Introduction To
Biostatistics
2

Key words :

Statistics , data , Biostatistics,
Variable ,Population ,Sample

3

Introduction
Some Basic concepts

Statistics is a field of study concerned
with
1- collection, organization, summarization
and analysis of data.
2- drawing of inferences about a body of
data when only a part of the data is
observed.
Statisticians try to interpret and
communicate the results to others.

4

* Biostatistics:

The tools of statistics are employed in
many fields:
business, education, psychology,
agriculture, economics, etc.
When the data analyzed are derived from
the biological science and medicine,
we use the term biostatistics to
distinguish this particular application of
statistical tools and concepts.
5
Data:
The raw material of Statistics is data.
We may define data as figures. Figures
result from the process of counting or
from taking a measurement.
For example:
- When a hospital administrator counts
the number of patients (counting).
- When a nurse weighs a patient
(measurement)
6

We search for suitable data to serve as
the raw material for our investigation.
Such data are available from one or more
of the following sources:
1- Routinely kept records.
For example:
- Hospital medical records contain
immense amounts of information on
patients.
- Hospital accounting records contain a
wealth of data on the facilitys business
- activities.

* Sources of Data:
7
2- External sources.
The data needed to answer a question may
already exist in the form of
published reports, commercially available
data banks, or the research literature,
i.e. someone else has already asked the
same question.

8
3- Surveys:
The source may be a survey, if the data
needed is about answering certain
questions.
For example:
If the administrator of a clinic wishes to
obtain information regarding the mode of
transportation used by patients to visit
the clinic,
then a survey may be conducted among
patients to obtain this information.
9
4- Experiments.
Frequently the data needed to answer
a question are available only as the
result of an experiment.
For example:
If a nurse wishes to know which of several
strategies is best for maximizing patient
compliance,
she might conduct an experiment in which the
different strategies of motivating compliance
are tried with different patients.
A Taxonomy of
Statistics
Two areas of statistics:

Descriptive Statistics: collection,
presentation, and description of sample
data.
Inferential Statistics: making
decisions and drawing conclusions about
populations.

11
Example: A recent study examined the math and verbal
SAT scores of high school seniors across the
country. Which of the following statements are
descriptive in nature and which are inferential.
1. The mean math SAT score was 492.
2. The mean verbal SAT score was 475.
3. Students in the Northeast scored higher in math but lower
in verbal.
4. 80% of all students taking the exam were headed for
college.
5. 32% of the students scored above 610 on the verbal SAT.
6. The math SAT scores are higher than they were 10 years
ago.
13

* A variable:
It is a characteristic that takes on
different values in different persons,
places, or things.
For example:
- heart rate,
- the heights of adult males,
- the weights of preschool children,
- the ages of patients seen in a dental
clinic.

Variable
- any characteristic of an individual
or entity. A variable can take
different values for different
individuals. Variables can be
categorical or quantitative.
14
Two kinds of variables:
Qualitative, or Attribute, or Categorical, Variable:
A variable that categorizes or describes an element of
a population.
Note: Arithmetic operations, such as addition and
averaging, are not meaningful for data resulting from a
qualitative variable.
Quantitative, or Numerical, Variable: A variable that
quantifies an element of a population.
Note: Arithmetic operations such as addition and
averaging, are meaningful for data resulting from a
quantitative variable.
16
Quantitative Variables
It can be measured
in the usual sense.
For example:
- the heights of
adult males,
- the weights of
preschool children,
- the ages of
patients seen in a
- dental clinic.
Qualitative Variables
Many characteristics are
not capable of being
measured. Some of them
can be ordered or
ranked.
For example:
- classification of people into
socio-economic groups,
- social classes based on
income, education, etc.
Types of variables
Quantitative

Qualitative

Example: Identify each of the following examples as attribute
(qualitative) or numerical (quantitative) variables.

1. The residence hall for each student in a statistics class.
(Attribute)
2. The amount of gasoline pumped by the next 10 customers at the
local Unimart. (Numerical)
3. The amount of radon in the basement of each of 25 homes in a
new development. (Numerical)
4. The color of the baseball cap worn by each of 20 students.
(Attribute)
5. The length of time to complete a mathematics homework
assignment. (Numerical)
6. The state in which each truck is registered when stopped and
inspected at a weigh station. (Attribute)
18
A discrete variable
is characterized by
gaps or interruptions
in the values that it
can assume.
For example:
- The number of daily
admissions to a
general hospital,
- The number of
decayed, missing or
filled teeth per child
- in an
- elementary
- school.
A continuous variable
can assume any value within a
specified relevant interval of
values assumed by the variable.
For example:
- Height,
- weight,
- skull circumference.
No matter how close together the
observed heights of two people,
we can find another person
whose height falls somewhere
in between.
Types of quantitative variables
Discrete

Continuous

In many cases, a discrete and continuous variable may
be distinguished by determining whether the variables
are related to a count or a measurement.
1. Discrete variables are
usually associated with
counting. If the
variable cannot be
further subdivided, it
is a clue that you are
probably dealing with a
discrete variable.

2. Continuous variables are
usually associated with
measurements. The
values of discrete
variables are only
limited by your ability
to measure them.

19
20
Measuring Variables
To establish relationships between
variables, researchers must observe the
variables and record their observations.
This requires that the variables be
measured.
The process of measuring a variable
requires a set of categories called a
scale of measurement and a process
that classifies each individual into one
category.
21
Nominal scale
- is an unordered set of categories
identified only by name. Nominal
measurements only permit you to determine
whether two individuals are the same or
different.
- Categorical variables with no inherent
order or ranking sequence such as names or
classes (e.g., gender). Value may be a
numerical, but without numerical value (e.g.,
I, II, III). The only operation that can be
applied to Nominal variables is enumeration.
Ordinal scale
- is an ordered set of categories.
Ordinal measurements tell you the
direction of difference between
two individuals.
- Variables with an inherent rank or
order, e.g. mild, moderate, severe.
Can be compared for equality, or
greater or less, but not how much
greater or less.
22
23
Interval scale
- is an ordered series of equal-sized categories.
Interval measurements identify the direction and
magnitude of a difference. The zero point is
located arbitrarily on an interval scale.
- Values of the variable are ordered as in Ordinal,
and additionally, differences between values are
meaningful, however, the scale is not absolutely
anchored. Calendar dates and temperatures on the
Fahrenheit scale are examples. Addition and
subtraction, but not multiplication and division are
meaningful operations.

Ratio scale
- is an interval scale where a value of zero
indicates none of the variable. Ratio
measurements identify the direction and
magnitude of differences and allow ratio
comparisons of measurements.
- Variables with all properties of Interval plus
an absolute, non-arbitrary zero point, e.g. age,
weight, temperature (Kelvin). Addition,
subtraction, multiplication, and division are all
meaningful operations.
24
Example: Identify each of the following as examples of
qualitative or numerical variables:

1. The temperature in Barrow, Alaska at 12:00 pm on any
given day.
2. The make of automobile driven by each faculty
member.
3. Whether or not a 6 volt lantern battery is defective.
4. The weight of a lead pencil.
5. The length of time billed for a long distance telephone call.
6. The brand of cereal children eat for breakfast.
7. The type of book taken out of the library by an adult.

Example: Identify each of the following as examples of
(1) nominal, (2) ordinal, (3) discrete, or (4) continuous
variables:
1. The length of time until a pain reliever begins to work.
2. The number of chocolate chips in a cookie.
3. The number of colors used in a statistics textbook.
4. The brand of refrigerator in a home.
5. The overall satisfaction rating of a new car.
6. The number of files on a computers hard disk.
7. The pH level of the water in a swimming pool.
8. The number of staples in a stapler.
27

* A population:
It is the largest collection of values of a
random variable for which we have an
interest at a particular time.
For example:
The weights of all the children enrolled in
a certain elementary school.
Populations may be finite or infinite.
28
* A sample:
It is a part of a population.
The weights of only a fraction of
these children.

Variable: A characteristic about each individual
element of a population or sample.
Data (singular): The value of the variable associated
with one element of a population or sample. This
value may be a number, a word, or a symbol.
Data (plural): The set of values collected for the
variable from each of the elements belonging to the
sample.
Experiment: A planned activity whose results yield a
set of data.
Parameter: A numerical value summarizing all the
data of an entire population.
Statistic: A numerical value summarizing the sample
data.

Example: A college dean is interested in learning about the
average age of faculty. Identify the basic terms in this
situation.

The population is the age of all faculty members at the college.
A sample is any subset of that population. For example, we
might select 10 faculty members and determine their age.
The variable is the age of each faculty member.
One data would be the age of a specific faculty member.
The data would be the set of values in the sample.
The experiment would be the method used to select the ages
forming the sample and determining the actual age of each
faculty member in the sample.
The parameter of interest is the average age of all faculty at
the college.
The statistic is the average age for all faculty in the sample.

31

ROOM
NO.
Gender Ages Job
Description
Civil
Status
Types of
Illness
101 M 25 Eng S HF
102 F 32 Clerk T M HF
103 F 38 HW M TF
104 M 46 Bank M M Cough
105 M 55 Taxi D M Pneu
106 F 32 Sec. W Pneu
107 M 60 Teacher W Diabetes
108 F 24 SalesClerk S Cancer
109 M 40 Bank T M HD
110 58 Postman Separated Hep.A
Chapter ( 2 )
Pages( 19 27)
Text Book : Basic Concepts and
Methodology for the Health
Sciences 33
Key words

frequency table, bar chart ,range
width of interval , mid-interval
Histogram , Polygon
Frequency Distribution
for Discrete Random Variables
Example:
Suppose that we take a
sample of size 16 from
children in a primary school
and get the following data
about the number of their
decayed teeth,
3,5,2,4,0,1,3,5,2,3,2,3,3,2,4,1
To construct a frequency
table:
1- Order the values from the
smallest to the largest.
0,1,1,2,2,2,2,3,3,3,3,3,4,4,5,5
2- Count how many
numbers are the same.
Relative
Frequency
Frequency No. of
decayed
teeth
0.0625
0.125
0.25
0.3125
0.125
0.125
1
2
4
5
2
2
0
1
2
3
4
5
1 16 Total
Sciences 35

Number of decayed teeth
5.00 4.00 3.00 2.00 1.00 .00
F
r
e
q
u
e
n
c
y
6
5
4
3
2
1
0
2 2
5
4
2
1
We can represent
the above simple
frequency table
using the bar
chart.
Sciences 36
2.3 Frequency Distribution
for Continuous Random Variables
For large samples, we cant use the simple frequency table to
represent the data.
We need to divide the data into groups or intervals or
classes.
So, we need to determine:
1- The number of intervals (k).
Too few intervals are not good because information will be
lost.
Too many intervals are not helpful to summarize the data.
A commonly followed rule is that 6 k 15,
or the following formula may be used,
k = 1 + 3.322 (log n)

Sciences 37
2- The range (R).
It is the difference between the
largest and the smallest observation
in the data set.
3- The Width of the interval (w).
Class intervals generally should be of
the same width. Thus, if we want k
intervals, then w is chosen such that
w R / k.

Sciences 38
Example:
Assume that the number of observations
equal 100, then
k = 1+3.322(log 100)
= 1 + 3.3222 (2) = 7.6 ~ 8.
Assume that the smallest value = 5 and the
largest one of the data = 61, then
R = 61 5 = 56 and
w = 56 / 8 = 7.
To make the summarization more
comprehensible, the class width may be 5
or 10 or the multiples of 10.

Sciences 39
2.3.1 Example
We wish to know how many class interval to have
in the frequency distribution of the data in Table
1.4.1 Page 9-10 of ages of 189 subjects who
Participated in a study on smoking cessation
Solution :
Since the number of observations
equal 189, then
k = 1+3.322(log 169)
= 1 + 3.3222 (2.276) ~ 9,
R = 82 30 = 52 and
w = 52 / 9 = 5.778

It is better to let w = 10, then the intervals
will be in the form:

Sciences 40
Frequency Class interval
11 30 39
46 40 49
70 50 59
45 60 69
16

70 79
1 80 89
189 Total
Sum of frequency
=sample size=n
41

The Cumulative Frequency:
It can be computed by adding successive
frequencies.

The Cumulative Relative Frequency:
It can be computed by adding successive relative
frequencies.

interval: - Mid The
It can be computed by adding the lower bound of
the interval plus the upper bound of it and then
divide over 2.
Sciences 42
For the above example, the following table represents the
cumulative frequency, the relative frequency, the cumulative
relative frequency and the mid-interval.
Cumulative
Relative
Frequency

Relative
Frequency
R.f
Cumulative
Frequency
Frequency
Freq (f)
Mid
interval
Class
interval
0.0582 0.0582 11
11
34.5
30 39
- 0.2434 57
46
44.5
40 49
0.6720 - 127
-
54.5
50 59
0.9101 0.2381 -
45
-
60 69
0.9948 0.0847 188
16

74.5
70 79
1 0.0053 189
1
84.5
80 89
1 189 Total
R.f= freq/n
Sciences 43
Example :
From the above frequency table, complete the
table then answer the following questions:
1-The number of objects with age less than 50
years ?
2-The number of objects with age between 40-69
years ?
3-Relative frequency of objects with age between
70-79 years ?
4-Relative frequency of objects with age more
than 69 years ?
5-The percentage of objects with age between
40-49 years ?

Sciences 44
6- The percentage of objects with age less than
60 years ?
7-The Range (R) ?
8- Number of intervals (K)?
9- The width of the interval ( W) ?

Sciences 45

Representing the grouped frequency table using
the histogram
To draw the histogram, the true classes limits should be used.
They can be computed by subtracting 0.5 from the lower
limit and adding 0.5 to the upper limit for each interval.

Frequency True class limits
11 29.5 <39.5
46 39.5 < 49.5
70 49.5 < 59.5
45 59.5 < 69.5
16

69.5 < 79.5
1 79.5 < 89.5
189
Total
0
10
20
30
40
50
60
70
80
34.5 44.5 54.5 64.5 74.5 84.5
Sciences 46
Representing the grouped frequency table
using the Polygon
0
10
20
30
40
50
60
70
80
34.5 44.5 54.5 64.5 74.5 84.5
Sciences 47
Exercises
Pages : 31 34
Questions: 2.3.2(a) , 2.3.5 (a)
H.W. : 2.3.6 , 2.3.7(a)
) : 2.4 Section (
Descriptive Statistics
Measures of Central
Tendency
41 - 38 Page
Methodology for the Health Sciences 49

key words:
Descriptive Statistic, measure of
central tendency ,statistic, parameter,
mean () ,median, mode.
50

A Statistic:
It is a descriptive measure computed from the
data of a sample.
A Parameter:
It is a a descriptive measure computed from
the data of a population.
Since it is difficult to measure a parameter from the
population, a sample is drawn of size n, whose
values are _
1
, _
2
, ,

_
n
. From this data, we
measure the statistic.

A measure of central tendency is a measure which
indicates where the middle of the data is.
The three most commonly used measures of central
tendency are:
The Mean, the Median, and the Mode.
The Mean:
It is the average of the data.
The Population Mean:

= which is usually unknown, then we use the

sample mean to estimate or approximate it.
The Sample Mean:
=

Example:
Here is a random sample of size 10 of ages, where
_
1
= 42, _
2
= 28, _
3
= 28, _
4
= 61, _
5
= 31,
_
6
= 23, _
7
= 50, _
8
= 34, _
9
= 32, _
10
= 37.

= (42 + 28 + + 37) / 10 = 36.6

x
1
N
i
i
N
X
=
x
1
n
i
i
n
x
=

Properties of the Mean:
Uniqueness. For a given set of data there is
one and only one mean.
Simplicity. It is easy to understand and to
compute.
Affected by extreme values. Since all
values enter into the computation.
Example: Assume the values are 115, 110, 119, 117, 121 and
126. The mean = 118.
But assume that the values are 75, 75, 80, 80 and 280. The
mean = 118, a value that is not representative of the set of
data as a whole.

The Median:
When ordering the data, it is the observation that divide the
set of observations into two equal parts such that half of
the data are before it and the other are after it.
* If n is odd, the median will be the middle of observations. It
will be the (n+1)/2
th
ordered observation.
When n = 11, then the median is the 6
th
observation.
* If n is even, there are two middle observations. The median
will be the mean of these two middle observations. It will
be the (n+1)/2
th
ordered observation.
When n = 12, then the median is the 6.5
th
observation, which
is an observation halfway between the 6
th
and 7
th
ordered
observation.
Example:
For the same random sample, the ordered
observations will be as:
23, 28, 28, 31, 32, 34, 37, 42, 50, 61.
Since n = 10, then the median is the 5.5
th

observation, i.e. = (32+34)/2 = 33.
Properties of the Median:
Uniqueness. For a given set of data there is
one and only one median.
Simplicity. It is easy to calculate.
It is not affected by extreme values as
is the mean.
It is the value which occurs most frequently.
If all values are different there is no mode.
Sometimes, there are more than one mode.
Example:
For the same random sample, the value 28 is
repeated two times, so it is the mode.
Sometimes, it is not unique.
It may be used for describing qualitative
data.

) : 2.5 Section (
Descriptive Statistics
Measures of Dispersion
46 - 43 Page

key words:
Descriptive Statistic, measure of
dispersion , range ,variance, coefficient of
variation.
2.5. Descriptive Statistics
Measures of Dispersion:
A measure of dispersion conveys information
regarding the amount of variability present in a set of
data.
Note:
1. If all the values are the same
There is no dispersion .
2. If all the values are different
There is a dispersion:
3.If the values close to each other
The amount of Dispersion small.
b) If the values are widely scattered
The Dispersion is greater.
43 Page 2.5.1 Ex. Figure
** Measures of Dispersion are :
1.Range (R).
2. Variance.
3. Standard deviation.
4.Coefficient of variation (C.V).
1.The Range (R):
Range =Largest value- Smallest value =

Note:
Range concern only onto two values
Example 2.5.1 Page 40:
Refer to Ex 2.4.2.Page 37
Data:
43,66,61,64,65,38,59,57,57,50.
Find Range?
Range=66-38=28
S L
x x
2.The Variance:
It measure dispersion relative to the scatter of the values
a bout there mean.
a) Sample Variance ( ) :
,where is sample mean

Example 2.5.2 Page 40:
Refer to Ex 2.4.2.Page 37
Find Sample Variance of ages , = 56
Solution:
S
2
= [(43-56)
2
+(66-43)
2
+..+(50-56)
2
]/ 10
= 900/10 = 90
x
2
S
1
) (
1
2
2
=
n
x x
S
n
i
i
x
b)Population Variance ( ) :
where , is Population mean
3.The Standard Deviation:
is the square root of variance=
a) Sample Standard Deviation = S =
b) Population Standard Deviation = =

2
o
N
x
N
i
i
=

=
1
2
2
) (
o
Varince
2
S
2
o
4.The Coefficient of Variation
(C.V):
Is a measure use to compare the
dispersion in two sets of data which is
independent of the unit of the
measurement .
where S: Sample standard
deviation.
: Sample mean.
) 100 ( .
X
S
V C =

X

: 46 Page 2.5.3 Example
Suppose two samples of human males yield the
following data:
Sampe1 Sample2
Age 25-year-olds 11year-olds
Mean weight 145 pound 80 pound
Standard deviation 10 pound 10 pound

We wish to know which is more variable.
Solution:
c.v (Sample1)= (10/145)*100= 6.9

c.v (Sample2)= (10/80)*100= 12.5

Then age of 11-years old(sample2) is more
variation

Exercises
Pages : 52 53
Questions: 2.5.1 , 2.5.2 ,2.5.3
H.W. : 2.5.4 , 2.5.5, 2.5.6, 2.5.14
* Also you can solve in the review
questions page 57:
Q: 12,13,14,15,16, 19

3 Chapter
Probability
The Basis of the
Statistical inference
Methodology for the Health Sciences
69

Key words:

Probability, objective Probability,
subjective Probability, equally likely
Mutually exclusive, multiplicative rule
Conditional Probability, independent events,
Bayes theorem
70
Introduction 3.1
The concept of probability is frequently encountered in
everyday communication. For example, a physician may
say that a patient has a 50-50 chance of surviving a certain
operation.
Another physician may say that she is 95 percent certain
that a patient has a particular disease.
Most people express probabilities in terms of percentages.
But, it is more convenient to express probabilities as
fractions. Thus, we may measure the probability of the
occurrence of some event by a number between 0 and 1.
The more likely the event, the closer the number is to one.
An event that can't occur has a probability of zero, and an
event that is certain to occur has a probability of one.
71
Two views of Probability 3.2
objective and subjective:
*** Objective Probability
** Classical and Relative
Some definitions:
1.Equally likely outcomes:
Are the outcomes that have the same
chance of occurring.
2.Mutually exclusive:
Two events are said to be mutually
exclusive if they cannot occur
simultaneously such that A B = .

72
The universal Set (S): The set all
possible outcomes.
The empty set : Contain no elements.
The event ,E : is a set of outcomes in S
which has a certain characteristic.
Classical Probability : If an event can
occur in N mutually exclusive and equally
likely ways, and if m of these possess a
triat, E, the probability of the occurrence
of event E is equal to m/ N .
For Example: in the rolling of the die ,
each of the six sides is equally likely to be
observed . So, the probability that a 4 will
be observed is equal to 1/6.
73
Relative Frequency Probability:
Def: If some posses is repeated a large
number of times, n, and if some resulting
event E occurs m times , the relative
frequency of occurrence of E , m/n will be
approximately equal to probability of E .
P(E) = m/n .
*** Subjective Probability :
Probability measures the confidence that a
particular individual has in the truth of a
particular proposition.
For Example : the probability that a cure
for cancer will be discovered within the
next 10 years.
74
Elementary Properties of 3.3
: Probability
Given some process (or experiment )
with n mutually exclusive events E
1
,
E
2
, E
3
,, E
n
, then
1-P(E
i
) 0, i= 1,2,3,n
2- P(E
1
)+ P(E
2
) ++P(E
n
)=1
3- P(E
i
+E
J
)= P(E
i
)+ P(E
J
),
E
i
,E
J
are mutually exclusive

75
Rules of Probability
1-Addition Rule
P(A U B)= P(A) + P(B) P (AB )

2- If A and B are mutually exclusive
(disjoint) ,then
P (AB ) = 0
Then , addition rule is
P(A B)= P(A) + P(B) .
3- Complementary Rule
P(A' )= 1 P(A)
where, A' = = complement event
Consider example 3.4.1 Page 63
76
Table 3.4.1 in Example 3.4.1
Total Later >18
(L)
Early = 18
(E)
Family history of
Mood Disorders
63 35 28
Negative(A)
57 38 19
Bipolar
Disorder(B)
85 44 41
Unipolar (C)
113 60 53
Unipolar and
Bipolar(D)
318 177 141 Total
77
**Answer the following questions:
Suppose we pick a person at random from this
sample.
1-The probability that this person will be 18-years old
or younger?
2-The probability that this person has family history of
mood orders Unipolar(C)?
3-The probability that this person has no family history
of mood orders Unipolar( )?
4-The probability that this person is 18-years old or
younger or has no family history of mood orders
Negative (A)?
5-The probability that this person is more than18-
years old and has family history of mood orders
Unipolar and Bipolar(D)?

C
78
Conditional Probability:

P(A\B) is the probability of A assuming
that B has happened.

P(A\B)= , P(B) 0

P(B\A)= , P(A) 0
) (
) (
B P
B A P
) (
) (
A P
B A P
79
64 Page 3.4.2 Example
From previous example 3.4.1 Page 63 ,
answer
suppose we pick a person at random and
find he is 18 years or younger (E),what is
the probability that this person will be one
who has no family history of mood
disorders (A)?
suppose we pick a person at random and
find he has family history of mood (D) what
is the probability that this person will be 18
years or younger (E)?
80
: Calculating a joint Probability
Example 3.4.3.Page 64
Suppose we pick a person at random
from the 318 subjects. Find the
probability that he will early (E) and
has no family history of mood
disorders (A).
81
Multiplicative Rule:
P(AB)= P(A\B)P(B)
P(AB)= P(B\A)P(A)
Where,
P(A): marginal probability of A.
P(B): marginal probability of B.
P(B\A):The conditional probability.
82
From previous example 3.4.1 Page
63 , we wish to compute the joint
probability of Early age at onset(E)
and a negative family history of
mood disorders(A) from a knowledge
of an appropriate marginal
probability and an appropriate
conditional probability.
Exercise: Example 3.4.5.Page 66
83
Independent Events:
If A has no effect on B, we said that
A,B are independent events.
Then,
1- P(AB)= P(B)P(A)
2- P(A\B)=P(A)
3- P(B\A)=P(B)
84
In a certain high school class consisting of
60 girls and 40 boys, it is observed that
24 girls and 16 boys wear eyeglasses . If a
student is picked at random from this
class ,the probability that the student
wears eyeglasses , P(E), is 40/100 or 0.4 .
What is the probability that a student
picked at random wears eyeglasses given
that the student is a boy?
What is the probability of the joint
occurrence of the events of wearing eye
glasses and being a boy?
85
Suppose that of 1200 admission to a
general hospital during a certain period of
time,750 are private admissions. If we
designate these as a set A, then compute
P(A) , P( ).

A
86
Marginal Probability:
Definition:
Given some variable that can be broken
down into m categories designated
by and another jointly occurring
variable that is broken down into n
categories designated by
, the marginal probability of with all the
categories of B . That is,
for all value of j
Example 3.4.9.Page 76
Use data of Table 3.4.1, and rule of
marginal Probabilities to calculate P(E).
= ), ( ) (
j i i
B A P A P
m i
A A A A ,......., ,......., ,
2 1
n j
B B B B ,......., ,......., ,
2 1
i
A
87
Exercise:
Page 76-77
Questions :
3.4.1, 3.4.3,3.4.4
H.W.
3.4.5 , 3.4.7
88
Baye's Theorem
Pages 79-83

89
Definition.1

The sensitivity of the symptom

This is the probability of a positive result given that the
subject has the disease. It is denoted by P(T|D)

Definition.2

The specificity of the symptom

This is the probability of negative result given that the
subject does not have the disease. It is denoted by

90
) ( ) | ( ) ( ) | (
) ( ) | (
) | (
D P D T P D P D T P
D P D T P
T D P
+
=
) | ( 1 ) | (
) ( 1 ) (
D T P D T p
D P D P
=
=
91
Definition.4

The predictive value negative of the symptom

This is the probability that a subject does not have the disease given that
the subject has a negative screening test result
It is calculated using Bayes Theorem through the following formula

where,

) ( ) | ( ) ( ) | (
) ( ) | (
) | (
D P D T P D P D T P
D P D T P
T D P
+
=
) | ( 1 ) | ( D T P D T p =
92
Example 3.5.1 page 82

A medical research team wished to evaluate a proposed screening test for
Alzheimers disease. The test was given to a random sample of 450
patients with Alzheimers disease and an independent random sample of
500 patients without symptoms of the disease. The two samples were
drawn from populations of subjects who were 65 years or older. The
results are as follows.
Test Result Yes (D) No ( ) Total
Positive(T) 436 5 441
Negativ( ) 14 495 509
Total 450 500 950
T
D
93
In the context of this example
a)What is a false positive?
A false positive is when the test indicates a positive result (T)
when the person does not have the disease

b) What is the false negative?
A false negative is when a test indicates a negative result ( )
when the person has the disease (D).

c) Compute the sensitivity of the symptom.

d) Compute the specificity of the symptom.

D
T
9689 . 0
450
436
) | ( = = D T P
99 . 0
500
495
) | ( = = D T P
94
e) Suppose it is known that the rate of the disease in the general
population is 11.3%. What is the predictive value positive of the symptom
and the predictive value negative of the symptom
The predictive value positive of the symptom is calculated as

The predictive value negative of the symptom is calculated
as

996 . 0
.113) (0.0311)(0 87) (0.99)(0.8
87) (0.99)(0.8

) ( ) | ( ) ( ) | (
) ( ) | (
) | (
=
+
=
+
=
D P D T P D P D T P
D P D T P
T D P
925 . 0
0.113) - (.01)(1 .113) (0.9689)(0
.113) (0.9689)(0

) ( ) | ( ) ( ) | (
) ( ) | (
) | (
=
+
=
+
=
D P D T P D P D T P
D P D T P
T D P
95
Exercise:
Page 83
Questions :
3.5.1, 3.5.2
H.W.:
Page 87 : Q4,Q5,Q7,Q9,Q21

Chapter 4:
Probabilistic features of
certain data Distributions
Pages 93- 111
Text Book : Basic Concepts and Methodology for the
Health Sciences
97
Key words

Probability distribution , random variable ,
Bernolli distribution, Binomail distribution,
Poisson distribution
Health Sciences
98
The Random Variable (X):

When the values of a variable (height,
weight, or age) cant be predicted in
advance, the variable is called a random
variable.

An example is the adult height.

When a child is born, we cant predict
exactly his or her height at maturity.
Health Sciences
99
4.2 Probability Distributions for
Discrete Random Variables
Definition:
The probability distribution of a
discrete random variable is a table,
graph, formula, or other device used
to specify all possible values of a
discrete random variable along with
their respective probabilities.

Health Sciences
100
The Cumulative Probability
Distribution of X, F(x):

It shows the probability that the
variable X is less than or equal to a
certain value, P(X s x).
Sciences 101
Example 4.2.1 page 94:
F(x)=
P(X x)
P(X=x) frequenc
y
Number of
Programs
0.2088 0.2088 62 1
0.3670 0.1582 47 2
0.4983 0.1313 39 3
0.6296 0.1313 39 4
0.8249 0.1953 58 5
0.9495 0.1246 37 6
0.9630 0.0135 4 7
1.0000 0.0370 11 8
1.0000 297 Total
Health Sciences
102
See figure 4.2.1 page 96
See figure 4.2.2 page 97

Properties of probability distribution
of discrete random variable.
1.
2.
3. P(a s X s b) = P(X s b) P(X s a-1)
4. P(X < b) = P(X s b-1)
0 ( ) 1 P X x s = s
( ) 1 P X x = =

Health Sciences
103
Example 4.2.2 page 96: (use table
in example 4.2.1)
What is the probability that a randomly
selected family will be one who used
three assistance programs?
in example 4.2.1)
selected family used either one or two
programs?
Health Sciences
104
Example 4.2.4 page 98: (use table in
example 4.2.1)
What is the probability that a family picked
at random will be one who used two or
fewer assistance programs?
example 4.2.1)
selected family will be one who used fewer
than four programs?
example 4.2.1)
selected family used five or more
programs?
Health Sciences
105
in example 4.2.1)
selected family is one who used
between three and five programs,
inclusive?
Health Sciences
106
The Binomial Distribution: 4.3
The binomial distribution is one of the most
widely encountered probability distributions
in applied statistics. It is derived from a
process known as a Bernoulli trial.
Bernoulli trial is :
When a random process or experiment
called a trial can result in only one of two
mutually exclusive outcomes, such as dead
or alive, sick or well, the trial is called a
Bernoulli trial.

Health Sciences
107
The Bernoulli Process
A sequence of Bernoulli trials forms a Bernoulli
process under the following conditions
1- Each trial results in one of two possible,
mutually exclusive, outcomes. One of the
possible outcomes is denoted (arbitrarily) as a
success, and the other is denoted a failure.
2- The probability of a success, denoted by p,
remains constant from trial to trial. The
probability of a failure, 1-p, is denoted by q.
3- The trials are independent, that is the outcome
of any particular trial is not affected by the
outcome of any other trial
Health Sciences
108
The probability distribution of the binomial
random variable X, the number of
successes in n independent trials is:

Where is the number of combinations
of n distinct objects taken x of them at a
time.

* Note: 0! =1

( ) ( ) , 0,1, 2,....,
X n X
n
f x P X x p q x n
x

| |
= = = =
|
|
\ .
n
x
| |
|
|
\ .
!
!( )!
n
n
x n x
x
| |
=
|
|

\ .
! ( 1)( 2)....(1) x x x x =
Health Sciences
109
Properties of the binomial
distribution
1.
2.
3.The parameters of the binomial
distribution are n and p
4.
5.

( ) 0 f x >
( ) 1 f x =
( ) E X np = =
2
var( ) (1 ) X np p o = =
Health Sciences
110
If we examine all birth records from the North
Carolina State Center for Health statistics for
year 2001, we find that 85.8 percent of the
pregnancies had delivery in week 37 or later
(full- term birth).
If we randomly selected five birth records from
this population what is the probability that
exactly three of the records will be for full-term
births?

Exercise: example 4.3.2 page 104
Health Sciences
111
Suppose it is known that in a certain
population 10 percent of the population is
color blind. If a random sample of 25
people is drawn from this population, find
the probability that
a) Five or fewer will be color blind.
b) Six or more will be color blind
c) Between six and nine inclusive will be color
blind.
d) Two, three, or four will be color blind.
Exercise: example 4.3.4 page 106
Health Sciences
112
The Poisson Distribution 4.4
If the random variable X is the number of
occurrences of some random event in a certain
period of time or space (or some volume of
matter).
The probability distribution of X is given by:
f (x) =P(X=x) = ,x = 0,1,..

The symbol e is the constant equal to 2.7183.
(Lambda) is called the parameter of the
distribution and is the average number of
occurrences of the random event in the interval
(or volume)
!
x
x
e


Health Sciences
113
Properties of the Poisson
distribution

1.
2.
3.
4.

( ) 0 f x >
( ) 1 f x =
( ) E X = =
2
var( ) X o = =
Health Sciences
114
In a study of a drug -induced anaphylaxis
among patients taking rocuronium bromide
as part of their anesthesia, Laake and
Rottingen found that the occurrence of
anaphylaxis followed a Poisson model with
=12 incidents per year in Norway .Find
1- The probability that in the next year,
among patients receiving rocuronium,
exactly three will experience anaphylaxis?

Health Sciences
115
2- The probability that less than two patients
receiving rocuronium, in the next year will
experience anaphylaxis?
3- The probability that more than two patients
receiving rocuronium, in the next year will
experience anaphylaxis?
4- The expected value of patients receiving
rocuronium, in the next year who will
experience anaphylaxis.
5- The variance of patients receiving
experience anaphylaxis
6- The standard deviation of patients receiving
experience anaphylaxis
Health Sciences
116
Example 4.4.2 page 111: Refer to
example 4.4.1
1-What is the probability that at least three
patients in the next year will experience
anaphylaxis if rocuronium is administered
with anesthesia?
2-What is the probability that exactly one
patient in the next year will experience
with anesthesia?
3-What is the probability that none of the
patients in the next year will experience
with anesthesia?
Health Sciences
117
4-What is the probability that at most
two patients in the next year will
experience anaphylaxis if rocuronium
is administered with anesthesia?

Exercises: examples 4.4.3, 4.4.4
and 4.4.5 pages111-113
Exercises: Questions 4.3.4 ,4.3.5,
4.3.7 ,4.4.1,4.4.5

4.5 Continuous
Probability Distribution
Pages 114 127

Text Book : Basic Concepts
and Methodology for the Health
Sciences
119
Key words:

Continuous random variable, normal
distribution , standard normal
distribution , T-distribution
Sciences
120
Now consider distributions of
continuous random variables.

Sciences
121
1- Area under the curve = 1.
2- P(X = a) = 0 , where a is a constant.
3- Area between two points a , b =
P(a<x<b) .

Properties of continuous
probability Distributions:

Sciences
122
4.6 The normal distribution:

It is one of the most important probability
distributions in statistics.
The normal density is given by
, - < x < , - < < , > 0

, e : constants
: population mean.
: Population standard deviation.

2
2
2
) (
2
1
) (
o
o t

=
x
e x f
Sciences
123
Characteristics of the normal
distribution: Page 111
The following are some important
characteristics of the normal distribution:
1- It is symmetrical about its mean, .
2- The mean, the median, and the mode are all
equal.
3- The total area under the curve above the
x-axis is one.
4-The normal distribution is completely
determined by the parameters and .
Sciences
124
5- The normal distribution
depends on the two
parameters and o.
determines the
location of
the curve.
(As seen in figure 4.6.3) ,

But, o determines
the scale of the curve, i.e.
the degree of flatness or
peaked ness of the curve.
(as seen in figure 4.6.4)
1
<
2
<
3
o
1
o
2
o
3
o
1
< o
2
< o
3
Sciences
125
Note that : (As seen in Figure
4.6.2)

1. P( - < x < + ) = 0.68
2. P( - 2< x < + 2)= 0.95
3. P( -3 < x < + 3) = 0.997
Sciences
126
The Standard normal
distribution:
Is a special case of normal distribution
with mean equal 0 and a standard deviation
of 1.
The equation for the standard normal
distribution is written as
, - < z <

2
2
2
1
) (
z
e z f

=
t
Sciences
127
Characteristics of the
standard normal distribution

1- It is symmetrical about 0.
2- The total area under the curve
above the x-axis is one.
3- We can use table (D) to find the
probabilities and areas.

Sciences
128
How to use tables of Z
Note that
The cumulative probabilities P(Z s z) are given in
tables for -3.49 < z < 3.49. Thus,
P (-3.49 < Z < 3.49) ~ 1.
For standard normal distribution,
P (Z > 0) = P (Z < 0) = 0.5
Example 4.6.1:
If Z is a standard normal distribution, then
1) P( Z < 2) = 0.9772
is the area to the left to 2
and it equals 0.9772.

2
Sciences
129
Example 4.6.2:
P(-2.55 < Z < 2.55) is the area between
-2.55 and 2.55, Then it equals
P(-2.55 < Z < 2.55) =0.9946 0.0054
= 0.9892.
Example 4.6.2:
P(-2.74 < Z < 1.53) is the area between
-2.74 and 1.53.
P(-2.74 < Z < 1.53) =0.9370 0.0031
= 0.9339.

-2.74 1.53
-2.55 2.55
0
Sciences
130
Example 4.6.3:
P(Z > 2.71) is the area to the right to 2.71.
So,
P(Z > 2.71) =1 0.9966 = 0.0034.

Example :
P(Z = 0.84) is the area at z = 2.71.
So,
P(Z = 0.84) =1 0.9966 = 0.0034
0.84
2.71
Sciences
131
How to transform normal
distribution (X) to standard
normal distribution (Z)?
This is done by the following formula:

Example:
If X is normal with = 3, = 2. Find the
value of standard normal Z, If X= 6?
Answer:

o

=
x
z
5 . 1
2
3 6
=
=
o
x
z

Sciences
132
The normal distribution can be used to model the distribution of
many variables that are of interest. This allow us to answer
probability questions about these random variables.
Example 4.7.1:
The Uptime is a custom-made light weight battery-operated
activity monitor that records the amount of time an individual
spend the upright position. In a study of children ages 8 to 15
years. The researchers found that the amount of time children
spend in the upright position followed a normal distribution with
Mean of 5.4 hours and standard deviation of 1.3.Find

Sciences
133
If a child selected at random ,then
1-The probability that the child spend less than 3
hours in the upright position 24-hour period

P( X < 3) = P( < ) = P(Z < -1.85) = 0.0322

-------------------------------------------------------------------------
2-The probability that the child spend more than 5

P( X > 5) = P( > ) = P(Z > -0.31)

= 1- P(Z < - 0.31) = 1- 0.3520= 0.648
-----------------------------------------------------------------------
3-The probability that the child spend exactly 6.2

P( X = 6.2) = 0

o
X
3 . 1
4 . 5 3
o
X
3 . 1
4 . 5 5
Sciences
134
4-The probability that the child spend from 4.5 to
7.3 hours in the upright position 24-hour period

P( 4.5 < X < 7.3) = P( < < )
= P( -0.69 < Z < 1.46 ) = P(Z<1.46) P(Z< -0.69)
= 0.9279 0.2451 = 0.6828

HwEX. 4.7.2 4.7.3
o
X
3 . 1
4 . 5 5 . 4
3 . 1
4 . 5 3 . 7
Sciences
135
1- It has mean of zero.
2- It is symmetric about the
mean.
3- It ranges from - to .

0
Sciences
136

4- compared to the normal distribution,
the t distribution is less peaked in the
center and has higher tails.
5- It depends on the degrees of freedom
(n-1).
6- The t distribution approaches the
standard normal distribution as (n-1)
approaches .

Sciences
137
Examples
t (7, 0.975) = 2.3646

------------------------------
t (24, 0.995) = 2.7696

--------------------------
If P (T
(18)
> t) = 0.975,
then t = -2.1009
-------------------------
If P (T
(22)
< t) = 0.99,
then t = 2.508
0.005
t
(24, 0.995)
0.995
t
(7, 0.975)
0.025
0.975
t

0.975
0.025
0.99
0.01
t

Sciences
138
Exercise:

Questions : 4.7.1, 4.7.2
H.W : 4.7.3, 4.7.4, 4.7.6
6 Chapter
Using sample data to make
estimates about population
) 172 - 162 parameters (P
Sciences 140
Key words:

Point estimate, interval estimate, estimator,
Confident level , , Confident interval for
mean , Confident interval for two means,
Confident interval for population proportion P,
Confident interval for two proportions

Sciences 141
6.1 Introduction:
Statistical inference is the procedure by which we
reach to a conclusion about a population on the basis
of the information contained in a sample drawn from
that population.
Suppose that:
an administrator of a large hospital is interested in
the mean age of patients admitted to his hospital
during a given year.
1. It will be too expensive to go through the records of
all patients admitted during that particular year.
2. He consequently elects to examine a sample of the
records from which he can compute an estimate of
the mean age of patients admitted to his that year.
Sciences 142
To any parameter, we can compute two types of
estimate: a point estimate and an interval estimate.
A point estimate is a single numerical value used to
estimate the corresponding population parameter.
An interval estimate consists of two numerical values
defining a range of values that, with a specified degree
of confidence, we feel includes the parameter being
estimated.
The Estimate and The Estimator:
The estimate is a single computed value, but the
estimator is the rule that tell us how to compute this
value, or estimate.
For example,

is an estimator of the population mean,. The
single numerical value that results from
evaluating this formula is called an estimate of
the parameter .
=
i
i
x x
Sciences 143
Confidence Interval for 6.2
a Population Mean: (C.I)
Suppose researchers wish to estimate the mean
of some normally distributed population.
They draw a random sample of size n from the
population and compute , which they use as a
point estimate of .
Because random sampling involves chance, then
cant be expected to be equal to .
The value of may be greater than or less
than .
It would be much more meaningful to estimate
by an interval.
x
x
Sciences 144
percent confidence o - 1 The
: interval (C.I.) for

We want to find two values L and U between which
lies with high probability, i.e.

P( L U ) = 1-o
Sciences 145
For example:
When,
o = 0.01,
then 1- o =
o = 0.05,
then 1- o =
o = 0.05,
then 1- o =
Sciences 146

We have the following cases
a) When the population is normal
1) When the variance is known and the sample size is large
or small, the C.I. has the form:
P( - Z
(1- o/2)
o/\n < < + Z
(1- o/2)
o/\n) = 1- o

2) When variance is unknown, and the sample size is small,
the C.I. has the form:

P( - t
(1- o/2),n-1
s/\n < < + t
(1- o/2),n-1
s/\n) = 1- o
x x
x x
Sciences 147
b) When the population is not
) 30 normal and n large (n>
1) When the variance is known the C.I. has
the form:
P( - Z
(1- o/2)
o/\n < < + Z
(1- o/2)
o/\n) = 1- o

2) When variance is unknown, the C.I. has
the form:
P( - Z
(1- o/2)
s/\n < < + Z
(1- o/2)
s/\n) = 1- o
x
x
x x
Sciences 148
Suppose a researcher , interested in obtaining an
estimate of the average level of some enzyme in a
certain human population, takes a sample of 10
individuals, determines the level of the enzyme in
each, and computes a sample mean of approximately
Suppose further it is known that the variable
of interest is approximately normally distributed with
a variance of 45. We wish to estimate . (o=0.05)
22 = x
Sciences 149
Solution:
1- o=0.95 o=0.05 o/2=0.025,
variance =
2
= 45 =\ 45,n=10
95%confidence interval for is given by:
P( - Z
(1- o/2)
o/\n < < + Z
(1- o/2)
o/\n) = 1- o
Z
(1- o/2)
= Z
0.975
= 1.96 (refer to table D)
Z
0.975
(o/\n) =1.96 (\ 45 / \10)=4.1578
22 1.96 (\ 45 / \10)
(22-4.1578, 22+4.1578) (17.84, 26.16)
Exercise example 6.2.2 page 169
22 = x
x x
Sciences 150
Example
The activity values of a certain enzyme measured in
normal gastric tissue of 35 patients with gastric
carcinoma has a mean of 0.718 and a standard
deviation of 0.511.We want to construct a 90 %
confidence interval for the population mean.
Solution:

Note that the population is not normal,
n=35 (n>30) n is large and o is unknown ,s=0.511
1- o=0.90 o=0.1
o/2=0.05 1-o/2=0.95,

Sciences 151
Then 90% confident interval for is given
by :
P( - Z
(1- o/2)
s/\n < < + Z
(1- o/2)
s/\n) = 1- o

Z
(1- o/2)
= Z
0.95
= 1.645 (refer to table D)
Z
0.95
(s/\n) =1.645 (0.511/ \35)=0.1421
0.718 1.645 (0.511) / \35
(0.718-0.1421, 0.718+0.1421)
(0.576,0.860).
Exercise example 6.2.3 page 164:
x
x
Sciences 152
Suppose a researcher , studied the effectiveness of
early weight bearing and ankle therapies following
acute repair of a ruptured Achilles tendon. One of the
variables they measured following treatment the
muscle strength. In 19 subjects, the mean of the
strength was 250.8 with standard deviation of 130.9
we assume that the sample was taken from is
approximately normally distributed population.
Calculate 95% confident interval for the mean of the
strength ?
Sciences 153
Solution:
1- o=0.95 o=0.05 o/2=0.025,
Standard deviation= S = 130.9 ,n=19
95%confidence interval for is given by:
P( - t
(1- o/2),n-1
s/\n < < + t
(1- o/2),n-1
s/\n) = 1- o
t
(1- o/2),n-1
= t
0.975,18
= 2.1009 (refer to table E)
t
0.975,18
(s/\n) =2.1009 (130.9 / \19)=63.1
250.8 2.1009 (130.9 / \19)
(250.8- 63.1 , 22+63.1) (187.7, 313.9)
Exercise 6.2.1 ,6.2.2
6.3.2 page 171

8 . 250 = x
x
x
Sciences 154
Confidence Interval for 6.3
the difference between two
Population Means: (C.I)
If we draw two samples from two independent population
and we want to get the confident interval for the
difference between two population means , then we have
the following cases :
1) When the variance is known and the sample sizes
is large or small, the C.I. has the form:

2
2
2
1
2
1
2
1
2 1 2 1
2
2
2
1
2
1
2
1
2 1
) ( ) (
n n
Z x x
n n
Z x x
o o

o o
o o
+ + < < +

Sciences 155
2) When variances are unknown but equal, and the
sample size is small, the C.I. has the form:

2
) 1 ( ) 1 (
1 1
) (
1 1
) (
2 1
2
2 2
2
1 1
2
2 1
) 2 ( ,
2
1
2 1 2 1
2 1
) 2 ( ,
2
1
2 1
2 1 2 1
+
+
=
+ + < < +
+ +
n n
S n S n
S
where
n n
S t x x
n n
S t x x
p
p
n n
p
n n
o o

Sciences 156
1) When the variance is known and the sample sizes is
large or small, the C.I. has the form:

2
2
2
1
2
1
2
1
2 1 2 1
2
2
2
1
2
1
2
1
2 1
) ( ) (
n
S
n
S
Z x x
n
S
n
S
Z x x + + < < +

o o

Sciences 157
Example 6.4.1 P174:
The researcher team interested in the difference between serum uric
and acid level in a patient with and without Downs syndrome .In a
large hospital for the treatment of the mentally retarded, a sample of
12 individual with Downs Syndrome yielded a mean of
mg/100 ml. In a general hospital a sample of 15 normal individual of
the same age and sex were found to have a mean value of
If it is reasonable to assume that the two population of values are
normally distributed with variances equal to 1 and 1.5,find the 95%
C.I for
1
-
2
Solution:
1- o=0.95 o=0.05 o/2=0.025 Z
(1- o/2)
= Z
0.975
= 1.96

1.11.96(0.4282) = 1.1 0.84 = ( 0.26 , 1.94 )
5 . 4
1
= x
4 . 3
2
= x
2
2
2
1
2
1
2
1
2 1
) (
n n
Z x x
o o
o
+
15
5 . 1
12
1
96 . 1 ) 4 . 3 5 . 4 ( + =
Sciences 158
Example 6.4.1 P178:
The purpose of the study was to determine the effectiveness of an
integrated outpatient dual-diagnosis treatment program for
mentally ill subject. The authors were addressing the problem of substance abuse
issues among people with sever mental disorder. A retrospective chart review was
carried out on 50 patient ,the recherch was interested in the number of inpatient
treatment days for physics disorder during a year following the end of the program.
Among 18 patient with schizophrenia, The mean number of treatment days was 4.7
with standard deviation of 9.3. For 10 subject with bipolar disorder, the mean
number of treatment days was 8.8 with standard deviation of 11.5. We wish to
construct 99% C.I for the difference between the means of the populations
Represented by the two samples

Sciences 159
Solution :
1- =0.99 = 0.01 /2 =0.005 1- /2 = 0.995
n
2
2 = 18 + 10 -2 = 26 + n
1
t
(1- o/2),(n1+n
2-2)
= t
0.995,26
= 2.7787, then 99% C.I for
1

2

where

then
(4.7-8.8) 2.7787 102.33 (1/18)+(1/10)
- 4.1 11.086 =( - 15.186 , 6.986)
Exercises: 6.4.2 , 6.4.6, 6.4.7, 6.4.8 Page 180
2 1
) 2 ( ,
2
1
2 1
1 1
) (
2 1 n n
S t x x
p
n n
+
+
o
33 . 102
2 10 18
) 5 . 11 9 ( ) 3 . 9 17 (
2
) 1 ( ) 1 (
2 2
2 1
2
2 2
2
1 1
2
=
+
+
=
+
+
=
x x
n n
S n S n
S
p
Sciences 160
Confidence Interval for a 6.5
Population proportion (P):
A sample is drawn from the population of interest ,then
compute the sample proportion such as

This sample proportion is used as the point estimator of
the population proportion . A confident interval is
obtained by the following formula

P
n
a
p = =
sample in the element of no. Total
istic charachtar some with sample in the element of no.
n
P P
Z P
)
1 (
2
1

o
Sciences 161
Example 6.5.1
The Pew internet life project reported in 2003 that 18%
of internet users have used the internet to search for
information regarding experimental treatments or
medicine . The sample consist of 1220 adult internet
users, and information was collected from telephone
interview. We wish to construct 98% C.I for the
proportion of internet users who have search for
information about experimental treatments or medicine
Sciences 162
Solution :
1- =0.98 = 0.02 /2 =0.01 1- /2 = 0.99
Z
1- /2
= Z
0.99
=2.33 , n=1220,
The 98% C. I is

0.18 0.0256 = ( 0.1544 , 0.2056 )

Exercises: 6.5.1 , 6.5.3 Page 187

18 . 0
100
18
= = p
1220
) 18 . 0 1 ( 18 . 0
33 . 2 18 . 0
)
1 (
2
1

n
P P
Z P
o
Sciences 163

Confidence Interval for the 6.6
difference between two Population
proportions :
Two samples is drawn from two independent population
of interest ,then compute the sample proportion for each
sample for the characteristic of interest. An unbiased
point estimator for the difference between two population
proportions
A 100(1-)% confident interval for P
1
- P
2
is given by

2 1

P P
2
2 2
1
1 1
2
1
2 1
)
1 (
1 (
)

(
n
P P
n
P P
Z P P

+

o
Sciences 164
Example 6.6.1
Connor investigated gender differences in proactive and
reactive aggression in a sample of 323 adults (68 female
and 255 males ). In the sample ,31 of the female and 53
of the males were using internet in the internet caf. We
wish to construct 99 % confident interval for the
difference between the proportions of adults go to
internet caf in the two sampled population .

Sciences 165
Solution :
1- =0.99 = 0.01 /2 =0.005 1- /2 = 0.995
Z
1- /2
= Z
0.995
=2.58 , n
F
=68, n
M
=255,

The 99% C. I is

0.2481 2.58(0.0655) = ( 0.07914 , 0.4171 )

2078 . 0
255
53
, 4559 . 0
68
31
= = = = = =
M
M
M
F
F
F
n
a
p
n
a
p
255
) 2078 . 0 1 ( 2078 . 0
68
) 4559 . 0 1 ( 4559 . 0
58 . 2 ) 2078 . 0 4559 . 0 (

+

M
M M
F
F F
M F
n
P P
n
P P
Z P P
)
1 (
1 (
)

(
2
1

o
Sciences 166
Exercises:
Questions :
6.2.1, 6.2.2,6.2.5 ,6.3.2,6.3.5, 6.4.2
6.5.3 ,6.5.4,6.6.1

Chapter 7
Using sample statistics to
Test Hypotheses
about population
parameters
Pages 215-233
168
Key words :

Null hypothesis H
0,
Alternative hypothesis H
A
,
testing hypothesis , test statistic , P-value

169
Hypothesis Testing

One type of statistical inference, estimation,
was discussed in Chapter 6 .

The other type ,hypothesis testing ,is discussed
in this chapter.
170
Definition of a hypothesis

It is a statement about one or more populations .
It is usually concerned with the parameters of
the population. e.g. the hospital administrator
may want to test the hypothesis that the average
length of stay of patients admitted to the
hospital is 5 days
171
Definition of Statistical hypotheses
They are hypotheses that are stated in such a way that
they may be evaluated by appropriate statistical
techniques.
There are two hypotheses involved in hypothesis
testing
Null hypothesis H
0
: It is the hypothesis to be tested .
Alternative hypothesis H
A
: It is a statement of what
we believe is true if our sample data cause us to reject
the null hypothesis
172
Testing a hypothesis about the 7.2
: mean of a population
We have the following steps:
1.Data: determine variable, sample size (n), sample
mean( ) , population standard deviation or sample
standard deviation (s) if is unknown
2. Assumptions : We have two cases:
Case1: Population is normally or approximately
normally distributed with known or unknown
variance (sample size n may be small or large),
Case 2: Population is not normal with known or
unknown variance (n is large i.e. n30).
x
173
3.Hypotheses:
we have three cases
Case I : H
0
: =
0
H
A
:
0

e.g. we want to test that the population mean is
different than 50
Case II : H
0
: =
0

H
A
: >
0

e.g. we want to test that the population mean is greater
than 50
Case III : H
0:
=
0

H
A
: <
0

e.g. we want to test that the population mean is less
than 50

==
174
4.Test Statistic:
Case 1: population is normal or approximately
normal

2
is known
2
is unknown
( n large or small)
n large n small

Case2: If population is not normally distributed and n is
large
i)If
2
is known ii) If
2
is unknown

n
X
Z
o

o
-
=

n
s
X
Z
o
-
=

n
s
X
T
o
-
=
n
s
X
Z
o
-
=
n
X
Z
o

o
-
=
175
5.Decision Rule:
i) If H
A
:
0

Reject H
0
if Z >Z
1-/2
or Z< - Z
1-/2
(when use Z - test)
Or Reject H
0
if T >t
1-/2,n-1
or T< - t
1-/2,n-1
(when use T- test)
__________________________
ii) If H
A
: >
0

Reject H
0
if Z>Z
1-
(when use Z - test)
Or Reject H
0
if T>t
1-,n-1
(when use T - test)
=
176
iii) If H
A
: <
0

Reject H
0
if Z< - Z
1-

(when use Z - test)
Or

Reject H
0
if T<- t
1-
,n-1
(when use T - test)
Note:
Z
1-/2
, Z
1-
, Z
are tabulated values obtained

from table D
t
1-/2
, t
1-
, t
are tabulated values obtained from

table E with (n-1) degree of freedom (df)
177

6.Decision :
If we reject H
0
, we can conclude that H
A
is
true.
If ,however ,we do not reject H
0
, we may
conclude that H
0
is true.

178
An Alternative Decision Rule using the
p - value Definition
The p-value is defined as the smallest value of
for which the null hypothesis can be rejected.
If the p-value is less than or equal to ,we
reject the null hypothesis (p )
If the p-value is greater than ,we do not
reject the null hypothesis (p > )

179
Example 7.2.1 Page 223
Researchers are interested in the mean age of a
certain population.
A random sample of 10 individuals drawn from the
population of interest has a mean of 27.
Assuming that the population is approximately
normally distributed with variance 20,can we
conclude that the mean is different from 30 years ?
(=0.05) .
If the p - value is 0.0340 how can we use it in making
a decision?
180
Solution
1-Data: variable is age, n=10, =27 ,
2
=20,=0.05
2-Assumptions: the population is approximately
normally distributed with variance 20
3-Hypotheses:
H
0
: =30
H
A
: 30
x
=
181
4-Test Statistic:
Z = -2.12
5.Decision Rule
The alternative hypothesis is
H
A
: > 30
Hence we reject H0 if Z >Z
1-0.025/2
= Z
0.975

or Z< - Z
1-0.025/2
= - Z
0.975
Z
0.975
=1.96(from table D)
182
6.Decision:

We reject H
0
,since -2.12 is in the rejection
region .

We can conclude that is not equal to 30

Using the p value ,we note that p-value
=0.0340< 0.05,therefore we reject H0
183
Example7.2.2 page227
Referring to example 7.2.1.Suppose that the
researchers have asked: Can we conclude
that <30.
1.Data.see previous example
2. Assumptions .see previous example
3.Hypotheses:
H
0
=30
H

A
: < 30

184
4.Test Statistic :

= = -2.12

5. Decision Rule: Reject H
0
if Z< Z
, where

Z
= -1.645. (from table D)

6. Decision: Reject H
0
,thus we can conclude that the
population mean is smaller than 30.
n
X
Z
o
o
-
=
10
20
30 27
185
Among 157 African-American men ,the mean
systolic blood pressure was 146 mm Hg with a
standard deviation of 27. We wish to know if
on the basis of these data, we may conclude
that the mean systolic blood pressure for a
population of African-American is greater than
140. Use =0.01.
186
Solution
1. Data: Variable is systolic blood pressure,
n=157 , =146, s=27, =0.01.
2. Assumption: population is not normal,
2
is
unknown
3. Hypotheses: H
0
:=140
H
A
: >140
4.Test Statistic:
= = = 2.78
n
s
X
Z
o
-
=
157
27
140 146
1548 . 2
6
187

5. Desicion Rule:
we reject H
0
if Z>Z
1-

= Z
0.99
= 2.33
(from table D)

6. Desicion: We reject H
0
.
Hence we may conclude that the mean systolic
blood pressure for a population of African-
American is greater than 140.
188
Hypothesis Testing :The Difference 7.3
: between two population mean
1.Data: determine variable, sample size (n), sample means,
population standard deviation or samples standard deviation
(s) if is unknown for two population.
2. Assumptions : We have two cases:
Case1: Population is normally or approximately normally
distributed with known or unknown variance (sample size
n may be small or large),
Case 2: Population is not normal with known variances (n
is large i.e. n30).
189
3.Hypotheses:
we have three cases
Case I : H
0
: 1 = 2
1
-
2
= 0

H
A
:
1

2

1
-

2
0

e.g. we want to test that the mean for first population is
different from second population mean.
Case II : H
0
: 1 = 2
1
-
2
= 0

H
A
:
1
>

2

1
-

2
>

0

e.g. we want to test that the mean for first population is
greater than second population mean.
Case III : H
0
: 1 = 2
1
-
2
= 0

H
A
:
1
<

2

1
-

2
< 0

e.g. we want to test that the mean for first population
is greater than second population mean.

190
4.Test Statistic:
Case 1: Two population is normal or approximately
normal

2
is known
2
is unknown if
( n
1
,n
2
large or small) ( n
1
,n
2
small)

population population Variances
Variances equal not equal

where
2
2
2
1
2
1
2 1 2 1
) ( - ) X - X (
n n
Z
o o

+
=

2 1
2 1 2 1
1 1
) ( - ) X - X (
n n
S
T
p
+
=

2
2
2
1
2
1
2 1 2 1
) ( - ) X - X (
n
S
n
S
T
+
=

2
) 1 (n ) 1 (n
2 1
2
2 2
2
1 1
2
+
+
=
n n
S S
S
p
191
Case2: If population is not normally distributed
and n
1,
n
2
is large(n
1
0 ,n
2
0)
and population variances is known,

2
2
2
1
2
1
2 1 2 1
) ( - ) X - X (
n n
Z
o o

+
=
192
5.Decision Rule:
i) If H
A
:
1

2

1
-

2
0

Reject H
0
if Z >Z
1-/2
or Z< - Z
1-/2
(when use Z - test)
Or Reject H
0
if T >t
1-/2 ,(n
1
+n
2
-2)
or T< - t
1-/2,,(n
1
+n
2
-2)

(when use T- test)
__________________________
ii) H
A
:
1
>

2

1
-

2
>

0

Reject H
0
if Z>Z
1-
(when use Z - test)
Or Reject H
0
if T>t
1-,(n
1
+n
2
-2)
(when use T - test)
193
iii) If H
A
:
1
<

2

1
-

2
< 0

Reject H
0
if Z< - Z
1-

(when use Z - test)
Or

Reject H
0
if T<- t
1-
, ,(n
1
+n
2
-2)
(when use T - test)
Note:
Z
1-/2
, Z
1-
, Z
are tabulated values obtained

from table D
t
1-/2
, t
1-
, t

table E with (n
1
+n
2
-2)

degree of freedom (df)
6. Conclusion: reject or fail to reject H
0
194
Researchers wish to know if the data have collected provide
sufficient evidence to indicate a difference in mean serum
uric acid levels between normal individuals and individual
with Downs syndrome. The data consist of serum uric
reading on 12 individuals with Downs syndrome from
normal distribution with variance 1 and 15 normal individuals
from normal distribution with variance 1.5 . The mean are
and =0.05.
Solution:
1. Data: Variable is serum uric acid levels, n
1
=12 , n
2
=15,
2
1
=1,
2
2
=1.5 ,=0.05.

100 / 5 . 4
1
mg X =
100 / 4 . 3
2
mg X =
195
2. Assumption: Two population are normal,
2
1
,
2
2

are known
3. Hypotheses: H
0
: 1 = 2
1
-
2
= 0

H
A
:
1

2

1
-

2
0

4.Test Statistic:
= = 2.57

5. Desicion Rule:
Reject H
0
if Z >Z
1-/2
or Z< - Z
1-/2

Z
1-/2=
Z
1-0.05/2=
Z
0.975=
1.96 (from table D)
6-Conclusion: Reject H
0
since 2.57 > 1.96
Or if p-value =0.102 reject H
0
if p < then reject H
0
2
2
2
1
2
1
2 1 2 1
) ( - ) X - X (
n n
Z
o o

+
=
15
5 . 1
12
1
) 0 ( - 3.4) - (4.5
+
=
196
Example7.3.2 page 240
The purpose of a study by Tam, was to investigate wheelchair
Maneuvering in individuals with over-level spinal cord injury (SCI)
And healthy control (C). Subjects used a modified a wheelchair to
incorporate a rigid seat surface to facilitate the specified
experimental measurements. The data for measurements of the
left ischial tuerosity ) ( for SCI and
control C are shown below
169 150 114 88 117 122 131 124 115 131 C
143 130 119 121 130 163 180 130 150 60 SCI
197

We wish to know if we can conclude, on the
basis of the above data that the mean of
left ischial tuberosity for control C lower
than mean of left ischial tuerosity for SCI,
equal Assume normal populations
1.33 - value = - , p 0.05 = . variances
198
Solution:
1. Data:, n
C
=10 , n
SCI
=10, S
C
=21.8, S
SCI
=133.1 ,=0.05.
, (calculated from data)
2.Assumption: Two population are normal,
2
1
,
2
2
are
unknown but equal
3. Hypotheses: H
0
:
C
=
SCI

C
-
SCI
= 0

H
A
:
C
<

SCI

C
-
SCI
< 0
4.Test Statistic:

Where,

1 . 126 =
C
X
1 . 133 =
SCI
X
569 . 0
10
1
10
1
04 . 756
0 ) 1 . 133 1 . 126 (
1 1
) ( - ) X - X (
2 1
2 1 2 1
=
+

=
+
=
n n
S
T
p

04 . 756
2 10 10
) 3 . 32 ( 9 ) 8 . 21 ( 9
2
) 1 (n ) 1 (n
2 2
2 1
2
2 2
2
1 1
2
=
+
+
=
+
+
=
n n
S S
S
p
199

5. Decision Rule:
Reject H
0
if T< - T
1-,(n
1
+n
2
-2)

T
1-,(n
1
+n
2
-2) =
T
0.95,18 =
1.7341 (from table E)

6-Conclusion: Fail to reject H
0
since -0.569 < - 1.7341
Or
Fail to reject H
0
since p = -1.33 > =0.05

200
Dernellis and Panaretou examined subjects with hypertension
and healthy control subjects .One of the variables of interest was
the aortic stiffness index. Measures of this variable were
calculated From the aortic diameter evaluated by M-mode and
blood pressure measured by a sphygmomanometer. Physics wish
to reduce aortic stiffness. In the 15 patients with hypertension
(Group 1),the mean aortic stiffness index was 19.16 with a
standard deviation of 5.29. In the30 control subjects (Group 2),the
mean aortic stiffness index was 9.53 with a standard deviation of
2.69. We wish to determine if the two populations represented by
these samples differ with respect to mean stiffness index .we wish
to know if we can conclude that in general a person with
thrombosis have on the average higher IgG levels than persons
without thrombosis at =0.01, p-value = 0.0559

201

Solution:
1. Data:, n
1
=53 , n
2
=54, S
1
= 44.89, S
2
= 34.85 =0.01.
2.Assumption: Two population are not normal,
2
1
,
2
2

are unknown and sample size large
3. Hypotheses: H
0
:
1
=
2

1
-
2
= 0

H
A
:
1
>

2

1
-
2
> 0
4.Test Statistic:

standard
deviation
Sample
Size
Mean LgG level Group
44.89 53 59.01 Thrombosis
34.85 54 46.61 No
Thrombosis
59 . 1
54
85 . 34
53
89 . 44
0 ) 61 . 46 01 . 59 ( ) ( - ) X - X (
2 2
2
2
2
1
2
1
2 1 2 1
=
+

=
+
=
n
S
n
S
Z

202

5. Decision Rule:
Reject H
0
if Z > Z
1-

Z
1- =
Z
0.99 =
2.33 (from table D)

6-Conclusion: Fail to reject H
0
since 1.59 > 2.33
Or
Fail to reject H
0
since p = 0.0559 > =0.01

203
Hypothesis Testing A single 7.5
: population proportion
Testing hypothesis about population proportion (P) is carried out
in much the same way as for mean when condition is necessary for
using normal curve are met
1.Data: sample size (n), sample proportion( ) , P
0

2. Assumptions :normal distribution ,
p
n
a
p = =
sample in the element of no. Total
istic charachtar some with sample in the element of no.

204
3.Hypotheses:
we have three cases
Case I : H
0
: P = P
0
H
A
: P P
0

Case II : H
0
: P = P
0

H
A
: P > P
0

Case III : H
0
: P = P
0

H
A
: P < P
0

4.Test Statistic:

Where H
0
is true ,is distributed approximately as the standard
normal

n
q p
p p
Z
0 0
0

=
205
5.Decision Rule:
i) If H
A
: P P
0

Reject H
0
if Z >Z
1-/2
or Z< - Z
1-/2

_______________________
ii) If H
A
: P> P
0

Reject H
0
if Z>Z
1-

_____________________________
iii) If H
A
: P< P
0

Reject H
0
if Z< - Z
1-

Note: Z
1-/2
, Z
1-
, Z

table D
0

206
2. Assumptions : is approximately normaly distributed
3.Hypotheses:
we have three cases
H
0
: P = 0.063
H
A
: P > 0.063
4.Test Statistic :

5.Decision Rule: Reject H
0
if Z>Z
1-
Where

Z
1-
= Z
1-0.05
=Z
0.95
= 1.645

21 . 1
301
) 0.937 ( 063 . 0
063 . 0 08 . 0
0 0
0
=
=
n
q p
p p
Z
p
207
6. Conclusion: Fail to reject H
0
Since
Z =1.21 > Z
1-=
1.645
Or ,
If P-value = 0.1131,
fail to reject H
0
P >

208
Wagen collected data on a sample of 301 Hispanic women
Living in Texas .One variable of interest was the percentage
of subjects with impaired fasting glucose (IFG). In the
study,24 women were classified in the (IFG) stage .The article
cites population estimates for (IFG) among Hispanic women
in Texas as 6.3 percent .Is there sufficient evidence to
indicate that the population Hispanic women in Texas has a
prevalence of IFG higher than 6.3 percent ,let =0.05
Solution:
1.Data: n = 301, p
0
= 6.3/100=0.063 ,a=24,
q
0
=1- p
0
= 1- 0.063 =0.937, =0.05
08 . 0
301
24
= = =
n
a
p
209
Hypothesis Testing :The 7.6
Difference between two
: population proportion
Testing hypothesis about two population proportion (P
1,,
P
2
) is
carried out in much the same way as for difference between two
means when condition is necessary for using normal curve are
met
1.Data: sample size (n
1
n
2
), sample proportions( ),
Characteristic in two samples (x
1
, x
2
),

2- Assumption : Two populations are independent .

2 1
P P
2 1
2 1
n n
x x
p
+
+
=
210
3.Hypotheses:
we have three cases
Case I : H
0
: P
1
= P
2
P
1
- P
2
= 0
H
A
: P
1
P
2
P
1
- P
2
0
Case II : H
0
: P
1
= P
2
P
1
- P
2
= 0
H
A
: P
1
> P
2
P
1
- P
2
> 0

Case III : H
0
: P
1
= P
2
P
1
- P
2
= 0
H
A
: P
1
< P
2
P
1
- P
2
< 0
4.Test Statistic:

Where H
0
is true ,is distributed approximately as the standard
normal

2 1
2 1 2 1
) 1 ( ) 1 (
) ( ) (
n
p p
n
p p
p p p p
Z

=
211
5.Decision Rule:
i) If H
A
: P
1
P
2

Reject H
0
if Z >Z
1-/2
or Z< - Z
1-/2

_______________________
ii) If H
A
: P
1
> P
2

Reject H
0
if Z >Z
1-

_____________________________
iii) If H
A
: P
1
< P
2
Reject H
0
if Z< - Z
1-

Note: Z
1-/2
, Z
1-
, Z

table D
0

212
Noonan is a genetic condition that can affect the heart growth,
blood clotting and mental and physical development. Noonan examined
the stature of men and women with Noonan. The study contained 29
Male and 44 female adults. One of the cut-off values used to assess
stature was the third percentile of adult height .Eleven of the males fell
below the third percentile of adult male height ,while 24 of the female
fell below the third percentile of female adult height .Does this study
provide sufficient evidence for us to conclude that among subjects with
Noonan ,females are more likely than males to fall below the respective
of adult height? Let =0.05
Solution:
1.Data: n
M
= 29, n
F
= 44 , x
M
= 11 , x
F
= 24, =0.05
479 . 0
44 29
24 11
=
+
+
=
+
+
=
F M
F M
n n
x x
p
545 . 0
44
24
, 379 . 0
29
11
= = = = = =
F
F
F
M
m
M
n
x
p
n
x
p
213
2- Assumption : Two populations are independent .
3.Hypotheses:
Case II : H
0
: P
F
= P
M
P
F
- P
M
= 0
H
A
: P
F
> P
M
P
F
- P
M
> 0

4.Test Statistic:

5.Decision Rule:
Reject H
0
if Z >Z
1-
, Where Z
1-
= Z
1-0.05
=Z
0.95
= 1.645
6. Conclusion: Fail to reject H
0
Since Z =1.39 > Z
1-=
1.645
Or , If P-value = 0.0823 fail to reject H
0
P >
39 . 1
29
) 521 . 0 )( 479 . 0 (
44
) 521 . 0 )( 479 . 0 (
0 ) 379 . 0 545 . 0 (
) 1 ( ) 1 (
) ( ) (
2 1
2 1 2 1
=
+

=

=
n
p p
n
p p
p p p p
Z
214
Exercises:
Questions : Page 234 -237
7.2.1,7.8.2 ,7.3.1,7.3.6 ,7.5.2 ,,7.6.1

H.W:
7.2.8,7.2.9, 7.2.11, 7.2.15,7.3.7,7.3.8,7.3.10
7.5.3,7.6.4


Chapter 9
Statistical Inference and The
Relationship between two variables

Prepared By : Dr. Shuhrat Khan
REGRESSION
CORRELATION
ANALYSIS OF VARIANCE

Regression, Correlation and Analysis of
Covariance are all statistical techniques that
use the idea that one variable say, may be
related to one or more variables through an
equation. Here we consider the relationship of
two variables only in a linear form, which is
called linear regression and linear correlation;
or simple regression and correlation. The
relationships between more than two
variables, called multiple regression and
correlation will be considered later.
Simple regression uses the relationship
between the two variables to obtain
information about one variable by knowing
the values of the other. The equation showing
this type of relationship is called simple linear
regression equation. The related method of
correlation is used to measure how strong the
relationship is between the two variables is.
216

EQUATION OF REGRESSION
Line of Regression
Simple Linear Regression:
Suppose that we are interested in a variable Y, but we want to
know about its relationship to another variable X or we want
to use X to predict (or estimate) the value of Y that might be
obtained without actually measuring it, provided the
relationship between the two can be expressed by a line. X is
usually called the independent variable and Y is called the
dependent variable.
We assume that the values of variable X are either fixed or

random. By fixed, we mean that the values are chosen by
researcher--- either an experimental unit (patient) is given this
value of X (such as the dosage of drug or a unit (patient) is
chosen which is known to have this value of X.
By random, we mean that units (patients) are chosen at
random from all the possible units,, and both variables X and
Y are measured.
We also assume that for each value of x of X, there is a whole
range or population of possible Y values and that the mean of
the Y population at X = x, denoted by
y/x
, is a linear
function of x. That is,

y/x
= +x

DEPENDENT VARIABLE
INDEPENDENT VARIABLE

TWO RANDOM VARIABLE
OR
BIVARIATE
RANDOM
VARIABLE
ESTIMATION
Estimate and .
Predict the value of Y at a
given value x of X.
Make tests to draw
conclusions about the model
and its usefulness.

We estimate the parameters
and by a and b
respectively by using sample
regression line:
= a+ bx
Where we calculate

We select a sample of
n observations (x
i
,y
i
)
from the population,
WITH
the goals


B =

ESTIMATION AND CALCULATION OF CONSTANTS , a AND b
EXAMPLE
investigators at a sports health centre are
interested in the relationship between oxygen
consumption and exercise time in athletes
recovering from injury. Appropriate mechanics
for exercising and measuring oxygen
consumption are set up, and the results are
presented below:
x variable

exercise
time
(min)

0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
y variable
oxygen consumption

620
630
800
840
840
870
1010
940
950
1130


calculations

o
r

Pearsons Correlation Coefficient

With the aid of Pearsons correlation coefficient (r),
we can determine the strength and the direction of
the relationship between X and Y variables,
both of which have been measured and they must
be quantitative.
For example, we might be interested in examining
the association between height and weight for the
following sample of eight children:
Height and weights of 8 children
Child Height(inches)X Weight(pounds)Y
A 49 81
B 50 88
C 53 87
D 55 99
E 60 91
F 55 89
G 60 95
H 50 90
Average ( = 54 inches) ( = 90 pounds)
Scatter plot for 8 babies
height weight
49 81
50 88
53 83
55 99
60 91
55 89
60 95
50 90
0
20
40
60
80
100
120
0 10 20 30 40 50 60 70
1
Table : The Strength of a Correlation

Value of r (positive or negative) Meaning
_______________________________________________________

0.00 to 0.19 A very weak correlation
0.20 to 0.39 A weak correlation
0.40 to 0.69 A modest correlation
0.70 to 0.89 A strong correlation
0.90 to 1.00 A very strong correlation
_______________________________________________________
_

FORMULA FOR CORRELATION
COEFFECIENT ( r )

With Pearsons r,
means that we add the products of the deviations to see if the positive
products or negative products are more abundant and sizable. Positive
products indicate cases in which the variables go in the same direction (that is,
both taller or heavier than average or both shorter and lighter than average);
negative products indicate cases in which the variables go in opposite
directions (that is, taller but lighter than average or shorter but heavier than
average).


Computational Formula for Pearsonss Correlation Coefficient r

Where SP (sum of the product), SSx (Sum of
the squares for x) and SSy (sum of the squares
for y) can be computed as follows:

Child X Y X
2
Y
2
XY

A 12 12 144 144 144
B 10 8 100 64 80
C 6 12 36 144 72
D 16 11 256 121 176
E 8 10 64 100 80
F 9 8 81 64 72
G 12 16 144 256 192
H 11 15 121 225 165

84 92 946 1118 981

Table 2 : Chest circumference and Birth
Weight of 10 babies

X(cm) y(kg) x
2
y
2
xy
___________________________________________________
22.4 2.00 501.76 4.00 44.8
27.5 2.25 756.25 5.06 61.88
28.5 2.10 812.25 4.41 59.85
28.5 2.35 812.25 5.52 66.98
29.4 2.45 864.36 6.00 72.03
29.4 2.50 864.36 6.25 73.5
30.5 2.80 930.25 7.84 85.4
32.0 2.80 1024.0 7.84 89.6
31.4 2.55 985.96 6.50 80.07
32.5 3.00 1056.25 9.00 97.5
TOTAL
292.1 24.8 8607.69 62.42 731.61

Checking for significance

There appears to be a strong between chest circumference and birth
weight in babies.
We need to check that such a correlation is unlikely to have arisen by
in a sample of ten babies.
Tables are available that gives the significant values of this correlation
ratio at two probability levels.
First we need to work out degrees of freedom. They are the number
of pair of observations less two, that is (n 2)= 8.
Looking at the table we find that our calculated value of 0.86 exceeds
the tabulated value at 8 df of 0.765 at p= 0.01. Our correlation is
therefore statistically highly significant.

Chapter 12
Analysis of Frequency Data
An Introduction to the Chi-Square
Distribution

TESTS OF INDEPENDENCE
To test whether two criteria of classification are
independent . For example socioeconomic status
and area of residence of people in a city are
independent.
We divide our sample according to status, low,
medium and high incomes etc. and the same
samples is categorized according to urban, rural or
suburban and slums etc.
Put the first criterion in columns equal in number
to classification of 1
st
criteria ( Socioeconomic
status) and the 2
nd
in rows, where the no. of rows
equal to the no. of categories of 2
nd
criteria (areas
of cities).
The Contingency Table
Table Two-Way Classification of sample
First Criterion of Classification

Second
Criterion

1

2

3

..

c

Total
1
2
3
.
.

r
N11
N21
N31
.
.

Nr1
N12
N22
N32
.
.

Nr2
N13
N 23
N33
.
.

Nr3

...
N1c
N2c
N3c
.
.

N rc
N1.
N2.
N3.
.
.

Nr.
Total N.1 N.2 N.3

N.c N
Observed versus Expected
Frequencies

Oi j : The frequencies in ith row and jth column given in
any contingency table are called observed frequencies
that result form the cross classification according to the
two classifications.
ei j :Expected frequencies on the assumption of
independence of two criterion are calculated by
multiplying the marginal totals of any cell and then
dividing by total frequency
Formula:

N
N N
e
j i
ij
) ( (
- -
=
Chi-square Test
After the calculations of expected frequency,
Prepare a table for expected frequencies and use Chi-
square

Where summation is for all values of r xc = k cells.
D.F.: the degrees of freedom for using the table are (r-
1)(c-1) for level of significance
Note that the test is always one-sided.

=
=
k
i
e
e o
i
i i
1
2
]
) (
[
2
_
Example 12.401(page 613)
The researcher are interested to determine that
preconception use of folic acid and race are
independent. The data is:
Observed Frequencies Table Expected
frequencies Table
Use of
Folic
Acid total

Yes
No
White
Black
Other
260
15
7
299
41
14
559
56
21
Total 282 354 636
Yes no Total
White

Black

Other
s
(282)(559)/636

=247.86

(282)(56)/636

=24.83
(282)((21)

=9.31
(354)(559)/63
6

=311.14

(354)(559)
=
31.17

21x354/636
=11.69
559

56

21
total 282 354 636
Calculations and Testing
091 . 9 69 . 11 / .....
14 . 311 / 86 . 247 /
) 69 . 11 14 (
) 14 . 311 299 ( ) 86 . 247 260 (
2
2 2 2
= + +
+ =
_
Data: See the given table
Assumption: Simple random sample
Hypothesis: H0: race and use of folic acid are independent
HA: the two variables are not independent. Let =
0.05
The test statistic is Chi Square given earlier
Distribution when H0 is true chi-square is valid with (r-1)(c-1)
= (3-1)(2-1)= 2 d.f.
Decision Rule: Reject H0 if value of is greater than

= 5.991

Calculations:
_
2
_
o
2
) 1 )( 1 ( , c r
Conclusion
Statistical decision. We reject H0 since 9.08960> 5.991

Conclusion: we conclude that H0 is false, and that there
is a relationship between race and preconception use of
folic acid.
P value. Since 7.378< 9.08960< 9.210, 0.01<p
<0.025
We also reject the hypothesis at 0.025 level of
significance but do not reject it at 0.01 level.
Solve Ex12.4.1 and 12.4.5 (p 620 & P 622)
ODDS RATIO
In a retrospective study, samples are selected from
those who have the disease called cases and those who
do not have the disease called controls . The
investigator looks back (have a retrospective look) at the
subjects and determines which one have (or had) and
which one do not have (or did not have ) the risk factor.
The data is classified into 2x2 table, for comparing cases
and controls for risk factor ODDS RATIO IS CALCULATED
ODDS are defined to be the ratio of probability of
success to the probability of failure.
The estimate of population odds ratio is
bc
ad
cld
b a
OR = =
/

ODDS RATIO
Where a, b, c and d are the numbers given in the
following table:

We may construct 100(1-)%CI for OR by formula:

Risk
Factor

Sample Total
Cases Control
Presen
t
a b a + b
Absent c d c + d
Total a + c b + d
R
X z
) / ( 1
2
2 / o

Example 12.7.2 for Odds Ratio
Example 12.5.7.2 page 640: Data relates
to the obesity status of children aged 5-6
and the smoking status of their mothers
during pregnancy
Hence OR for table
is :

Obesity status

Smoking
status(during
Pregnancy)
cases Non-
cases
Total
Smoked
throughout
64 342 406
Never smoked 68 3496 3564
Total 132 3838 3970
62 . 9
) 68 )( 342 (
) 3496 )( 64 (
= = OR
Confidence Interval for Odds
Ratio
The (1-) 100% Confidence Interval for Odds Ratio is:

Where

For Example 12.5.7.2 we have: a=64, b=342, c=68,
d=3496 , therefore:

Its 95% CI is:

or (7.12, 13.00)

) )( )( )( (
) (
2
2
d b c b d a c a
bc ad n
X
+ + + +

=
=
R O
X z
)
2
/ ( 1
o
68 . 217
) 3564 )( 406 )( 3833 )( 132 (
) 68 342 3496 64 (
2
3970
2
=

=
X
62 . 9
)
6831 . 217
/ 96 . 1 ( 1
R O
X z
)
2
/ ( 1
o

Interpretation of Example 12.7.2
Data
The 95% confidence interval (7.12, 13.00)
mean that we are 95% confident that the
population odds ratio is somewhere between
7.12 and 13.00
Since the interval does not contain 1, in fact
contains values larger than one, we conclude
that, in Pop. Obese children (cases) are more
likely than non-obese children ( non-cases) to
have had a mother who smoked throughout
the pregnancy.
Solve Ex 12.7.4 (page 646)

Interpretation of ODDS RATIO
The sample odds ratio provides an estimate
of the relative risk of population in the case
of a rare disease.
The odds ratio can assume values between 0
to .
A value of 1 indicate no association between
risk factor and disease status.
A value greater than one indicates increased
odds of having the disease among subjects in
whom the risk factor is present.
246
Chapter 13
Special Techniques for use
when population parameters
and/or population distributions
are unknoen
pages 683-689

247
NON-PARAMETRIC STATISTICS
The t-test, z-test etc. were all parametric
tests as they were based n the
assumptions of normality or known
variances.

When we make no assumptions about the
sample population or about the population
parameters the tests are called non-
parametric and distribution-free.
248
ADVANTAGES OF NON-PARAMETRIC
STATISTICS
Testing hypothesis about simple statements
(not involving parametric values) e.g.
The two criteria are independent (test for
independence)
The data fits well to a given distribution (goodness
of fit test)
Distribution Free: Non-parametric tests may
be used when the form of the sampled
population is unknown.
Computationally easy
Analysis possible for ranking or categorical
data (data which is not based on
measurement scale )

249
The Sign Test

This test is used as an alternative to t-
test, when normality assumption is not
met
The only assumption is that the
distribution of the underlying variable
(data) is continuous.
Test focuses on median rather than mean.
The test is based on signs, plus and
minuses
Test is used for one sample as well as for
two samples

250
Example
(One Sample Sign Test)
Score of 10
mentally retarded girls

We wish to know
if Median of population is
different from 5.
Solution:
Data: is about scores of 10
mentally retarded girls
Assumption: The measurements are continuous variable.

Girl Scor
e
Gi
rl
Score
1
2
3
4
5
4
5
8
8
9
6
7
8
9
10
6
10
7
6
6
251
Continued.
Hypotheses: H0: The population median is 5
HA: The population median is not 5
Let = 0.05
Test Statistic: The test statistic for the sign
test is either the observed number of plus signs
or the observed number of minus signs. The
nature of the alternative hypothesis determines
which of these test statistics is appropriate. In a
given test, any one of the following alternative
hypotheses is possible:
HA: P(+) > P(-) one-sided alternative
HA: P(+) < P(-) one-sided alternative
HA: P(+) P(-) two-sided alternative

252
Continued.

If the alternative hypothesis is HA: P(+) > P(-) a
sufficiently small number of minus signs causes
rejection of H0. The test statistic is the number of
minus signs.
If the alternative hypothesis is HA: P(+) < P(-) a
sufficiently small number of plus signs causes
rejection of H0. The test statistic is the number of
plus signs.
If the alternative hypothesis is HA: P(+) P(-)
either a sufficiently small number of plus signs or
a sufficiently small number of minus signs causes
rejection of the null hypothesis. We may take as
the test statistic the less frequently occurring
sign.
253
Continued.
Distribution of test statistic: If we assign
a plus sign to those scores that lie above the
hypothesized median and a minus to those
that fall below.

Decision Rule: Let k = minimum of pluses
or minuses. Here k = 1, the minus sign.
For HA: P(+) > P(-) reject H0 if, when H0 if
true, the probability of observing k or fewer
minus signs is less than or equal to .
Girl 1 2 3 4 5 6 7 8 9 1
0
Score relative
to median = 5

-

0

+

+

+

+

+

+

+

+
254
Continued.

For HA: P(+) > P(-) reject H0 if, when H0 if true,
the probability of observing k or fewer minus
signs is less than or equal to .
For HA: P(+) < P(-), reject H0 if the probability of
observing, when H0 is true, k or fewer plus signs
is equal to or less than .
For HA: P(+) P(-) , reject H0 if (given that H0 is
true) the probability of obtaining a value of k as
extreme as or more extreme than was actually
computed is equal to or less than /2.
Calculation of test statistic: The probability of
observing k or fewer minus signs when given a
sample of size n and parameter p by evaluating
the following expression:
P (X k | n, p) =

q p
C
x n x
k
x
n
x
0
255
Continued.

For our example we would compute

Statistical decision: In Appendix Table B we
find
P (k 1 | 9, 0.5) = 0.0195
Conclusion: Since 0.0195 is less than 0.025, we
reject the null hypothesis and conclude that the
median score is not 5.
p value: The p value for this test is 2(0.0195) =
0.0390, because it is two-sided test.

0195 . 0 01758 . 0 00195 . 0
) 5 . 0 ( ) 5 . 0 ( ) 5 . 0 ( ) 5 . 0 (
1 9 1
9
1
0 9 0
9
0
= + =
+

C C
256
SIGN TEST----Paired Data
This is used an alternative to t-test for paired
observations, when the underlying assumptions
of t test are not met.
Null Hypothesis to be tested the median
difference is zero.
OR
P (Xi > Yi ) = P (Yi > Xi )
Subtract Yi from Xi , if Yi is less than Xi , the
sign of the difference is (+), if Yi is greater
than Xi , the sign of the difference is ( - ), so
that
H
0
: P(+) = P(-) = 0.5
TEST STATISTIC: As before is k, the no of least
occurring of Plus or minus signs.

257
SIGN TEST----Example 13.3.2
A dental research team matched 12 pairs of 24 patients in age,
sex, intelligence. Six months later random evaluation showed
the following score (low score score is higher level of hygiene)

H0 : P(+) = P(-) = 0.5

1.Data. Scores of dental hygiene, one member instructed
how to brush and other remained uninstructed.
2. Assumption: the variable of dist is continues
3. Ho : The median of the difference is zero [P(+) =P(-)]
HA : The median of the difference is negative
[P(+) <P(-)]

pair no.

1 2 3 4 5 6 7 8 9 10 11 12
instructed 1.5 2.0 3.5 3.0 3.5 2.5 2.0 1.5 1.5 2.0 3.0 2.0
Not
instructed
2.0 2.0 4.0 2.5 4.0 3.0 3.5 3.0 2.5 2.5 2.5 2.5
Difference - 0 - + - - - - - - + -
258
Continued.
Let be 0.05
4. Test Statistic: The test statistic is the number of
plus signs which occurs less frequent. i.e. k = 2
5. Distribution of k is binomial with n= 11 (as one
observation is discarded) and p= 0.5
6. Decision Rule: Reject H0 if P(k2| 11,0.5) 0.05.
7. Calculations:
P(k2/11,0.5)=
Table B or calculations show the probability is
equal to 0.0327 which is less than 0.05, we
must reject H0 .
8. Conclusion: median difference is negative and
instructions are beneficial
9. p value: Since it is one sided test the p-value is
p= .0327

( )
) 5 . 0 ( ) 5 . 0
11
2
0
11
(
k k
k
k

=

259
NON-PARAMETRIC STATISTICS
The t-test, z-test etc. were all parametric
tests as they were based n the
assumptions of normality or known
variances.

When we make no assumptions about the
sample population or about the population
parameters the tests are called non-
parametric and distribution-free.
260
EXAMPLE 1
Cardiac output (liters/minute) was measured by
thermodilution in a simple random sample of 15
postcardiac surgical patients in the left lateral
position. The results were as follows:

We wish to know if we can conclude on the basis of
these data that the population mean is different
from 5.05.
Solution:
1. Data. As given above
2. Assumptions. We assume that the requirements
for the application of the Wilcoxon signed-ranks test
are met.
3. Hypothesis.
H0: = 5.05
HA: 5.05
Let = 0.05.

4.91 4.10 6.74 7.27 7.42 7.50 6.56 4.64
5.98 3.14 3.23 5.80 6.17 5.39 5.77
261
EXAMPLE 1
4. Test Statistic. The test statistic will be T + or T-
, whichever is smaller, called the test statistic T.
5. Distribution of test statistic. Critical values of
the test statistic are given in Table K of the
Appendix.
6. Decision rule. We will reject H0 if the computed
value of T is less than or equal to 25, the critical
value n = 15, and /2 = 0.0240, the closest value
to 0.0250 in Table K.
7. Calculation of test statistic. The calculation of
the test statistic is shown in Table.
8. Statistical decision. Since 34 is greater than
25, we are unable to reject H0.

262

Cardiac
output
di = xi
5.05
Rank of |di | Signed Rank of |di
|
4.91 -0.14 1 -1
4.10 -0.95 7 -7
6.74 +1.69 10 +10
7.27 +2.22 13 +13
7.42 +2.37 14 +14
7.50 +2.45 15 +15
6.56 +1.51 9 +9
4.64 -0.41 3 -3
5.98 +0.93 6 +6
3.14 -1.91 12 -12
3.23 -1.82 11 -11
5.80 +0.75 5 +5
6.17 +1.12 8 +8
5.39 +0.34 2 +2
5.77 +0.72 4 +4
T+ = 86, T- = 34, T = 34
263
EXAMPLE 1
8. Statistical decision. Since 34 is greater than
25, we are unable to reject H0.
9. Conclusion. We conclude that the population
mean may be 5.05
10. p value. From Table K we see that the p value
is p = 2(0.0757) = 0.1514

264
EXAMPLE 2
A researcher designed an experiment to assess the
effects of prolonged inhalation of cadmium oxide. Fifteen
laboratory animals served as experimental subjects,
while 10 similar animals served as controls. The variable
of interest was hemoglobin level following the
experiment. The results are shown in Table 2.
We wish to know if we can conclude that prolonged
inhalation of cadmium oxide reduces hemoglobin level.

265
EXAMPLE 2
TABLE 2. HEMOGLOBIN DETERMINATIONS (GRAMS) FOR 25
LABORATORY ANIMALS
EXPOSED ANIMALS (X) UNEXPOSED ANIMALS
(Y)
14.4 17.4
14.2 16.2
13.8 17.1
16.5 17.5
14.1 15.0
16.6 16.0
15.9 16.9
15.6 15.0
14.1 16.3
15.3 16.8
15.7
16.7
13.7
15.3
14.0
266
EXAMPLE 2
Solution:
1. Data. See table above
2. Assumptions. We presume that the
assumptions of the Mann-Whitney test are met.
3. Hypothesis.
H0: Mx My
HA: Mx < My

where Mx is the median of a population of animals
exposed to cadmium oxide and My is the median of
a population of animals not exposed to the
substance. Suppose we let = 0.05.

267
EXAMPLE 2

4. Test Statistic. The test statistic is

where n is the number of sample X observations
and S is the sum of the ranks assigned to the
sample observations from the population of X
values. The choice of which samples values we
label as X is arbitrary.

2
) 1 ( +
=
n n
S T
268

Sum of the Y ranks = S = 145
TABLE 2. ORIGINAL DATA AND RANKS
X 13.7 13.8 14.0 14.1 14.1 14.2 14.4 15.3 15.3 15.6
Rank 1 2 3 4.5 4.5 6 7 10.5 10.5 12
Y 15.0 15.0
Rank 8.5 8.5
X 15.7 15.9 16.
5
16.
6
16.
7
Ran
k
13 14 18. 19 20
Y 16.0 16.
2
16.
3
16.
8
16.
9
17.
1
17.
4
17.
5
Ran
k
15 16 17 21 22 23 24 25
269
EXAMPLE 2

5. Distribution of test statistic. The critical
values are given in Table K.
6. Decision Rule. Reject H0: Mx My, if the
computed T is less than w with n, the number of X
observations; m the number of Y observations and
, the chosen level of significance.
If the null hypothesis were of the types

H0: Mx My
HA: Mx > My

Reject H0: Mx My if the computed T is greater
than w1-, where W1- = nm - W .

270
EXAMPLE 2

For the two-sided test situation with

H0: Mx = My
HA: Mx My

Reject H0: Mx = My if the computed value of T is
either less than w/2 or greater than w1-/2 , where
w/2 is the critical value of T for n, m and /2 given
in Appendix II Table K and w1-/2 = nm - w/2.
For this example the decision rule of T is smaller
than 45, the critical value of the test statistic for n
= 15, m = 10, and = 0.05 found in Table K.

271
EXAMPLE 2

7. Calculation of test statistic. We have S = 145,
so that

8. Statistical Decision. When we enter Table K
with n = 15, m = 10, and = 0.05, we find the
critical value of w1- to be 45. Since 25 is less than
45, we reject H0.
9. Conclusion. We conclude that Mx is smaller than
MY. This leads us to the conclusion that prolonged
inhalation of cadmium oxide does reduce the
hemoglobin level.
Since 22< 25 < 30, we have for this test
0.005 > p >0.001.

25
2
) 1 15 ( 15
145 =
+
= T
272
EXAMPLE 2

When either n or m is greater than 20 we cannot
use Appendix Table K to obtain critical values for
the Mann-Whitney test. When this is the case we
may compute

And compare the result, for significance, with
critical values of the standard normal distribution.

12 / ) 1 (
2 /
+ +
=
m n nm
mn T
z

Introduction To Biostatistics

Uploaded by

Copyright:

Available Formats

Introduction To Biostatistics

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Introduction To Biostatistics

Uploaded by

Copyright:

Available Formats

1

Text Book : Basic Concepts and

Text Book : Basic Concepts and

Text Book : Basic Concepts and Methodology for the

Text Book : Basic Concepts and Methodology for the

Text Book : Basic Concepts and Methodology for the

are tabulated values obtained

are tabulated values obtained from

= -1.645. (from table D)

are tabulated values obtained

are tabulated values obtained from

Text Book : Basic Concepts and

are tabulated values obtained from

are tabulated values obtained from

We assume that the values of variable X are either fixed or

Text Book : Basic Concepts and

Text Book : Basic Concepts and

Text Book : Basic Concepts and

You might also like